CRYZL1 (NM_005111) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	4P11, QOH-1; 2210407J23Rik; 2410006O11Rik; crystallin, zeta (quinone reductase)-like 1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC116933 sequence for NM_005111 edited (data generated by NextGen Sequencing) ATGAAAGGCTTATATTTCCAACAGAGTTCCACAGATGAAGAAATAACATTTGTATTTCAA GAAAAGGAAGATCTTCCTGTTACAGAGGATAACTTTGTGAAACTTCAAGTTAAAGCTTGT GCTCTGAGCCAGATAAATACAAAGCTTCTGGCAGAAATGAAGATGAAAAAGGATTTATTT CCTGTTGGGAGAGAAATTGCTGGAATTGTATTAGATGTTGGAAGCAAGGTATCATTCTTT CAACCAGATGATGAAGTAGTTGGAATTTTGCCCCTGGACTCTGAAGACCCTGGACTTTGT GAAGTTGTTAGAGTACATGAGCATTACTTGGTTCATAAACCAGAAAAGGTCACATGGACG GAAGCAGCAGGAAGCATTCGGGATGGAGTGCGTGCCTATACAGCTCTGCATTATCTTTCT CATCTCTCTCCTGGAAAATCAGTGCTGATAATGGATGGAGCAAGTGCATTTGGTACAATA GCTATTCAGTTAGCACATCATAGAGGAGCCAAAGTGATTTCAACAGCATGCAGCCTTGAA GATAAGCAGTGCCTTGAAAGATTCAGACCTCCCATAGCCCGAGTGATTGATGTATCTAAT GGGAAAGTTCATGTTGCTGAAAGCTGTTTGGAAGAAACAGGTGGCCTGGGAGTAGATATT GTCCTAGATGCTGGAGTGAGATTATATAGTAAAGATGATGAACCAGCTGTAAAACTACAA CTACTACCACATAAACATGATATCATCACACTTCTTGGTGTTGGAGGCCACTGGGTAACA ACAGAAGAAAACCTTCAGTTGGATCCTCCAGATAGCCACTGCCTTTTCCTCAAGGGAGCA ACGTTAGCTTTCCTGAATGATGAAGTTTGGAATTTGTCAAATGTACAACAGGGAAAATAT CTTTGTATCTTAAAGGATGTGATGGAGAAGTTATCAACTGGTGTTTTCAGACCTCAGTTG GATGAACCCATTCCACTGTATGAGGCAAAAGTTTCCATGGAAGCTGTTCAGAAAAATCAA GGAAGAAAAAAGCAAGTTGTTCAATTTTAA Clone variation with respect to NM_005111.5 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_005111 unedited GATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGTGAAGGTGCA GGCCGTTGGGGCGGCTCAGAGGCAGGTGACTATGAAAGGCTTATATTTCCAACAGAGTTT CACAGATGAAGAAATAACATTTGTATTTCAAGAAAAGGAAGATCTTCCTGTTACAGAGGA TAACTTTGTGAAACTTCAAGTTAAAGCTTGTGCTCTGAGCCAGATAAATACAAAGCTTCT GGCAGAAATGAAGATGAAAAAGGATTTATTTCCTGTTGGGAGAGAAATTGCTGGAATTGT ATTAGATGTTGGAAGCAAGGTATCATTCTTTCAACCAGATGATGAAGTAGTTGGAATTTT GCCCCTGGACTCTGAAGACCCTGGACTTTGTGAAGTTGTTAGAGTACATGAGCATTACTT GGTTCATAAACCAGAAAAGGTCACATGGACGGAAGCAGCAGGAAGCATTCCGGATGGAGT GCGTGCCTATACAGCTCTGCATTATCTTTCTCATCTCTCTCCTGGAAAATCAGTGCTGAT AATGGATGGAGCAAGTGCATTTGGTACAATAGCTATTCAGTTAGCACATCATAGAGGAGC CAAAGTGATTTCAACAGCATGCAGCCTTGAAGATAAGCAGTGCCTTGAAAGATTCAGACC TCCCATAGCCCGAGTGATTGATGTATCTAATGGGAAAGTTCATGTTGCTGAAAGCTGTTT GGAAGAACAGGTGGCCTGGGAGTAGATATTGTCCTAGATGCTGGAGTGAGATTATATAGT AAAGATGATGAACCAGCTGTTAAACTACCACTACTACCCACCTAAACATGATATCATCAC ACTTCTTGGTTGTGGNAGGCCACTGGGTACAACAGAAGAAAACCTTCAGTTGGATCCTNC CGATAGCCACTGCCTTTTNCTTCAGGGAGCACGCTAGCTCTTCTGAATGATGAAGTTGGN AATTGTCCAAGTACACCAGGGACA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_005111 unedited CTTTCTATGNACCGCAGGCCGCTATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTAAAGGGCCTATGGGCTAAGTCTTTTAAAACTTCAAAATATA CTCAAATGCTTAAATTATGACAGCAAAAACTTAAGGAAGGGGTTGAAAACGGAGTTGATA CAAAATTAATACGTTACTTTGATAGCTTAACAAAGGCCTGCAACAGCTTTTATCAAAAGT AGGGGGACTTTTGCTGAAAGCAGCAGTTTTCTTCATGGAAGGAATTTTATAGCAGGGGCA AAATATATAGAAACAATGAAAAGGTTTTTAAAAAAATTCCCTTAAAATGTTAATTATAAA ATTATCTTGATCATTTGCACAAAAATTTTTCAAACACTTTTCAAATGTGTCAACTGAGCA TCTTGTCTTAACAAAAAAACGTGCTTAAAAATGCAACTTGTTTTATCTTCCTTGGTTTTT CTCAACAATTTCCAAAAAAAATTCTGGCTTCAAATACTGGAATATGTTCATCCGACTGAG GTCTGAAAAAAAAAAAAATTAAAATTGAACAACTTGCTTTTTTCTTCCTTGATTTTTCTG AACAGCTTCCATGGAAACTTTTGCCTCATACAGGGGAATGGGTTCATCCAACTGAGGTCT GAAAACACCAGTTGATAACTTCTCCATCACATCCTTTAAGAACAAAGATATTTTCCCTGT TGTACATTTGACAAATTCCAAACCTCATCATTCAAGAAAGCTAACGTTGCTCCCTTGAAG AAAAAGCAGTGGCTATCTGGAGGATCCACTGAAGTTTTCTTCTGTTGTACCCAGTGGCCT CCACACCAAAAAGGTGATGATATATGTTTATGTGGAGTAGTTGGAGTTCACAGCGGTTCA TATCTTTACTAATAATCCCCTCCACATCTAGGAAATTTCTACTCCAGCCCACTTGTTCTT CCAACAAT
Restriction Sites	NotI-NotI
ACCN	NM_005111
Insert Size	1680 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_005111.5, NP_005102.2
RefSeq Size	1639 bp
RefSeq ORF	1050 bp
Locus ID	9946
Domains	ADH_zinc_N
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein that has sequence similarity to zeta crystallin, also known as quinone oxidoreductase. This zeta crystallin-like protein also contains an NAD(P)H binding site. Alternatively spliced transcript variants have been observed but their full-length nature has not been completely determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the shortest transcript yet it encodes the longer isoform (a).

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