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HIV TAT specific factor 1 (HTATSF1) (NM_014500) Human Untagged Clone
CAT＃: SC114990
HTATSF1 (untagged)-Human HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | dJ196E23.2; TAT-SF1; TATSF1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114990 sequence for NM_014500 edited (data generated by NextGen Sequencing)
ATGAGCGGCACCAACTTGGATGGGAACGATGAGTTTGATGAGCAGTTGCGAATGCAAGAA TTGTACGGAGACGGCAAGGATGGTGACACCCAGACCGATGCCGGCGGAGAACCCGATTCT CTCGGGCAGCAGCCGACGGACACTCCCTACGAGTGGGACCTGGACAAAAAGGCTTGGTTC CCCAAGATTACTGAAGATTTCATTGCTACATATCAGGCCAATTATGGCTTCTCTAACGAT GGCGCATCTAGTTCTACCGCAAATGTTGAAGATGTCCATGCTAGGACTGCAGAGGAACCT CCACAAGAAAAAGCCCCGGAACCCACTGATGCCAGAAAGAAGGGAGAAAAAAGAAAGGCT GAGTCAGGATGGTTTCATGTTGAAGAAGACAGAAATACAAATGTATACGTGTCTGGTTTG CCTCCAGATATTACAGTGGATGAATTTATACAACTTATGTCCAAGTTTGGCATTATTATG AGAGATCCTCAGACAGAAGAATTTAAGGTCAAACTTTACAAAGATAATCAAGGAAATCTT AAAGGAGACGGTCTTTGCTGTTATTTGAAAAGAGAATCTGTGGAACTTGCATTAAAACTT TTGGATGAAGATGAAATTAGAGGCTACAAATTACATGTTGAGGTGGCAAAGTTTCAACTG AAGGGAGAATATGATGCCTCAAAGAAGAAGAAGAAGTGCAAAGACTATAAGAAGAAGCTG TCTATGCAACAAAAGCAGTTGGATTGGAGACCTGAGAGGCGAGCCGGACCATCCCGGATG CGCCATGAGCGAGTTGTCATCATCAAGAATATGTTTCATCCTATGGATTTTGAGGATGAT CCGTTGGTGCTGAATGAGATCAGAGAAGACCTTCGAGTAGAGTGTTCGAAGTTTGGACAA ATTAGGAAACTCCTTCTCTTTGATAGGCACCCAGATGGTGTGGCCTCTGTGTCCTTTCGG GATCCAGAGGAAGCTGATTATTGTATTCAGACTCTCGATGGAAGATGGTTTGGTGGCCGT CAAATCACTGCCCAGGCATGGGATGGGACTACAGATTATCAGGTGGAGGAAACCTCAAGA GAAAGGGAGGAAAGGCTGAGAGGATGGGAGGCTTTCCTCAATGCTCCTGAGGCCAACAGA GGCCTTAGGCGTTCAGATTCTGTCTCTGCTTCCGAAAGGGCAGGGCCTTCTAGAGCAAGG CATTTTTCAGAGCACCCCAGCACATCTAAAATGAATGCTCAAGAAACTGCAACTGGAATG GCGTTTGAAGAACCTATAGATGAGAAGAAGTTTGAAAAGACAGAAGATGGGGGAGAATTT GAAGAAGGTGCTTCTGAAAACAATGCTAAGGAAAGTAGCCCCGAAAAAGAGGCTGAAGAA GGCTGCCCTGAAAAAGAATCTGAAGAGGGCTGCCCCAAAAGAGGGTTTGAAGGCAGCTGC TCCCAAAAAGAGTCTGAAGAAGGCAATCCCGTAAGAGGATCTGAAGAGGATAGTCCTAAA AAAGAGTCTAAAAAGAAGACACTCAAAAATGATTGTGAAGAGAATGGCCTTGCAAAGGAA TCTGAAGATGACCTCAACAAGGAGTCTGAAGAGGAGGTTGGCCCCACAAAAGAGTCCGAA GAAGATGACTCAGAGAAAGAGTCTGATGAAGACTGCTCTGAAAAACAGTCTGAAGATGGC TCCGAAAGAGAATTTGAAGAAAATGGTCTCGAGAAAGATTTGGATGAGGAAGGTTCTGAA AAGGAGCTTCATGAAAATGTTCTTGACAAAGAGTTAGAAGAAAATGACTCTGAAAACTCC GAATTTGAAGATGACGGCTCTGAAAAAGTGTTAGATGAGGAAGGCTCTGAGAGAGAGTTT GACGAAGATTCAGATGAAAAGGAAGAAGAGGAGGATACATATGAAAAAGTATTTGATGAT GAGTCTGATGAGAAAGAGGATGAAGAATATGCAGATGAAAAGGGGCTTGAAGCTGCTGAT AAAAAGGCGGAAGAAGGTGATGCAGATGAAAAGCTGTTTGAAGAGTCAGATGACAAGGAA GATGAAGATGCAGATGGAAAGGAAGTTGAAGATGCTGACGAAAAGTTGTTCGAAGATGAT GATTCCAATGAGAAGTTGTTTGATGAGGAGGAAGATTCCAGTGAGAAGTTGTTTGACGAT TCTGATGAGAGGGGGACTTTGGGTGGTTTTGGGAGTGTTGAAGAAGGGCCCCTATCCACT GGCAGCAGCTTTATTCTCAGTAGCGATGATGATGACGATGATATTTAA Clone variation with respect to NM_014500.4 1725 c=>t >OriGene 5' read for NM_014500 unedited
TATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAAACATGAGCGGCACCAACT TGGATGGGAACGATGAGTTTGATGAGCAGTTGCGAATGCAAGAATTGTACGGAGACGGCA AGGATGGTGACACCCAGACCGATGCCGGCGGAGAACCCGATTCTCTCGGGCAGCAGCCGA CGGACACTCCCTACGAGTGGGACCTGGACAAAAAGGCTTGGTTCCCCAAGATTACTGAAG ATTTCATTGCTACATATCAGGCCAATTATGGCTTCTCTAACGATGGCGCATCTAGTTCTA CCGCAAATGTTGAAGATGTCCATGCTAGGACTGCAGAGGAACCTCCACAAGAAAAAGCCC CGGAACCCACTGATGCCAGAAAGAAGGGAGAAAAAAGAAAGGCTGAGTCAGGATGGTTTC ATGTTGAAGAAGACAGAAATACAAATGTATACGTGTCTGGTTTGCCTCCAGATATTACAG TGGATGAATTTATACAACTTATGTCCAAGTTTGGCATTATTATGAGAGATCCTCAGACAG AAGAATTTAAGGTCAAACTTTACAAAGATAATCAAGGAAATCTTAAAGGAGACGGTCTTT GCTGTTATTTGAAAAGAGAATCTGTGGAACTTGCATTANAACTTTTGGATGAAGATGAAA TTAGAGGCTANCAATTACATGGTTGAGGTGGNCAAAGTTTCAACTGAAGGGAGAATATTG ATGCCTCNAAGAGAAGAANGAAGTGCAAGACTATAGNAGAAAGCTGTCTATGAAACAAAA GCAGTTGATGGNAGACCTGAAAGCGAGCCGGACCATCCGGNATGCGCATGAGACGATTGN CATATCAGAAATTGGTTCATCTTAGGATTGAGGAAAACCCCTGGTCTGAATGAACAAAAA AACCTTGAAGNAAAGGTNCAAGTTGGACAATTGGAACTTCCTCTTGATAGCCC >OriGene 3' read for NM_014500 unedited
GGGTTTCCTCCCCTTTTCTTTTATTGTCGCAACCCCACATACTTTACTCACTCCCCTCAA AATATTATTGTGCATGCATCTATCAAATTTGACACAAGCACAAGTCTCCAGTTTACTTGC ATTGTAACTATGACATTGTAACTAAACAATTAATTAACGTATATTCAAAACCATTATGTA CAGGTAACAATGAAACACTTGAATGGTCTAATGTGATGGCATACTACCACTATGCACATG TCGCATGATAACGACTAGCTAGTTGATCTGATCCCGTGCAATGCACTATGGGCGACATGT CGACACGTGGAGGCACCTCTAAGCTTCACATCCCCATGCGTTCATCCGTGACCATATATT TCGCCCCGTCCATTGTATCATCCCGATTCCTTGATAATGCAGCTCTGTCCGACCATGTGT CATCCCGGTGCCTCTTGCTTGTAACACTCCCGGAGAAACGTCGCTTACGGCGTTTCTGTC CGCCGAGACTTGTCCCCGCGTCGTCGGTCGACTGCGACTGTTCGCGCCTATTCCGCCTCA ACCGTCAGTTCGGCGGCCGACTGTTTGTATGCGTATCACTATGTTCACCTTCACCATCGC GCCGGCCCCCCTATCTCCGACATAATATTATTTAACTTAGATTAATAGTCCGTCGCACCG TATGAAGTCTGTGATTGTTGGTCGCCTTTTGCGCCATCCCGTCTCGCGCAGCCGTATCCC ATTCCATATTACGTTTTATTTTTTGTTCCTTTCGTCCGTCCATGTGATCCCCCGCTTCCC GCCCTTTTCTTCCCTCTTTTCCGTTATCCGTTCGTCCCGTCTTTCCATGTGTATCCCGTG CCGCGCTCTTCGCTCTTCCCATTTTATTCGTTCCCGCTCCCCCGCCCCTTACGCATACCC GTATGTCCGCTCTCCCTNCTCCGAGTCCGTGTTCGTCTCCGACCCCGCAGTGTTTTGCAC CCCTTCGCTCCCGTCGTTCCCCGATCCCTCGTTGTATCAACCGAACCGTGCTNCCACCTT TGATCCGCTTCGCATTGTCTCCTGTTCTTTGCCGCCTCGCGCGACGTCCTCTGTTCACGC GTGGTCGCTCNATACGTCGCCGCCCGTCTCTGCGATGTCGGTATCTCACCATCCCTCTCC ATTATAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014500 |
| Insert Size | 2840 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014500.3, NP_055315.2 |
| RefSeq Size | 2776 bp |
| RefSeq ORF | 2268 bp |
| Locus ID | 27336 |
| UniProt ID | O43719 |
| Domains | RRM |
| Protein Families | Transcription Factors |
| Gene Summary | The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Sep 2009] Transcript Variant: This variant (2) is missing a 5' non-coding exon, and therefore, has a different 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The pre-mRNA splicing and transcription factor Tat-SF1 is a functional partner of the spliceosome SF3b1 subunit via a U2AF homology motif interface
,null,
The Journal of Biological Chemistry
,PubMed ID 30567737
[HTATSF1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200601 | HTATSF1 (Myc-DDK-tagged)-Human HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2 |
CNY 5,800.00 |
|
| RC200601L3 | Lenti-ORF clone of HTATSF1 (Myc-DDK-tagged)-Human HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2 |
CNY 7,700.00 |
|
| RC200601L4 | Lenti-ORF clone of HTATSF1 (mGFP-tagged)-Human HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2 |
CNY 7,700.00 |
|
| RG200601 | HTATSF1 (tGFP-tagged) - Human HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2 |
CNY 6,370.00 |
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