Vcan (NM_053663) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cspg2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217768 representing NM_053663 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAAATATGAACGGCATCCTATGGATGTGCTCAACCTTACTGTTAACGCATGCACTGCATAAAG CCAAAATGGAAGAAAACCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGATCCTACCTTGTCATTTTTC AACCTTGCCCACCTTACCACCCGATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAAATAGAA GTGGACAAAAATGGAAAAGACATAAAGGAGACTACTGTCCTGGTGGCCCAAGACGGGAACATCAAGATTG GTCAGGACTACAAGGGGCGGGTATCAGTGCCTACGCATCCCGATGACGTAGGCGATGCCTCTCTCACCAT GGTCAAACTCCGTGCTAGTGACGCAGGTGTCTACCGCTGTGATGTCATGTATGGCATTGAAGACACTCAG AACACGATGTCGCTGGCCGTGGACGGTGTCGTGTTTCACTACAGGGCAGCGACCAGCAGATACACTCTGA ACTTCGAGTCTGCTCAACAGGCTTGTTTGGACATCGGGGCGGTCATAGCAACCCCAGAGCAGCTGTTCGC TGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGACCAAACTGTCAGATATCCCATA CGGGCTCCCCGAGAGGGCTGTTATGGAGACATGATGGGGAAGGAAGGGGTCCGGACCTATGGATTCCGCT CTCCCCAGGAAACCTATGATGTGTATTGCTATGTGGATCATCTGGACGGCGATGTGTTCCACATCACTGC TCCCAGTAAATTCACCTTCGAGGAGGCCGAAGCAGAGTGTGCAAACCGGGATGCCAGGCTGGCGACTGTT GGGGAACTTCACGCAGCTTGGAGGAACGGCTTTGACCAGTGCGATTACGGCTGGCTATCGGATGCCAGCG TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACGTCGAATG AGTGATATGGTTGTAAATGGTCATCCAATAGATTCAGAATCTAAAGAAGAGGAACCTTGCAGTGAAGAAA CAGATCCACTGCATGATCTGTTTGCTGAAATTTTACCCGAGTTACCAGATTCCTTCGAAATAGACATATA TCACAGTGAGGAAGATGAAGATGGAGAGGAGGACTGTGTAAATGCAACTGATGTAACAACCACTCCGTCA GTGCAGTACATCACTGGGAAGCCACATGTCACTACAGTGCCCAAAAACCCAGAAGCTGCAGAAGCGAGGC GTGGCCTGTATGAAAGTGTTGCGCCTTCTCAGAATTTCTCAAATACTTCCGCAACTGACACCCATCAGTT TATACCGGCCGAAACAGAATTGTCAACTACCATGCAATTTACCAAATCCAAAGAAGCCACTGAATTGTTA GAAATCACATGGAAACCCGAGACCTACCCTGAAACACCAGAACACTTTTCAAGTGGTGAGCCTGATGTTT TCCCTACACTGCCATCCCATGATGGTAAAACCACCAAATGGTCAGAGTTCATCACAGAGAGCAATCCAAA CACAGAAAATCCAGAACACAAACAACCTAAGCCTATACCTCTGTTTCCTGAAGAGTTTTCAGGAGAGGGT GCCATTGACCAAGCATCCCAGCAAACAATCTTTTCCACGGCTACAGAAGTAGCACTTGGTAAAGAGACAG ACCAAAGTCCTACCATATCTACCTCTAGCATACGTTCAGGTTCTGTGTCAGTACATGCCTTGGAGGAAGA TCCAATTGCTCTAACAGGAATCTCACAGACTGATGAATCCATGTCTACTGTGGAAAGCTGGGTAGAGATG ACCCCTAGCCAAACTGTAGAGTTCTCTGGGAGTTCTTCAGCTCCTACTATTGAAGGGTCTGGGGAAGTAG AAGAATATACAAATAAAATTTTCAACACGGTAACTGATTTGCCACAGAGAGAGCCTACAGATACGCTCAT CCCCTTGGATATGAGCAACATCATGATCACAGACCACCACATTTATACTCCTGCAACCACCGCTCCTTTG GATTCACAGCTACCTTCTACAGATGCACGGCCTACACAATTTGGAATTCAAACCACCACCTCTGAGTGGG TTTCCAGTACATCTTTTGAAGGAAGGAAAACAGAGGAAGACAAAGAAAGAGATACAAATGCAGCCCACAC AGGGGAAGTCCAACCAGCGACAGAGAGGTCAGATCGCTTACTCTTAACTTCAGAATTAGAAAGTTCAAAT GTGGCTGCATCTAGTCCTTTGGACACCTGGGAAGGTTTTGTGCCAGAGACAACATCAACAGTGTCCGAAA AGGAAATGGCAAACACAACTCCTGTTTTTACAGAAACAAGTGATGTAGCCAATTTGGAGACACAGTCTTT TGAGCATAGTAGTAGCAGTCAGCCCAGGGTACAGGAAGAGCTGACCACTCTCTCAGGAAAGCCCCCCTTG ATTTTTATGGACCTGGGTTCAGGAGATGCTAGTACTGATATGGAATTCATCACTGCTTCTTCATTTACAT TAGATTTGGAGTCCGATACCAAAGTCAAAAAAGAATTACCCAGTACTCTGTCTCCCAGTGTGGAGACTTC ATCCTCCTCTGAGCCAATAGGATTAGCTCCGAGTACTGTATTGGACATAGAAATTGTGGAAGTTATGAAT CAGACATCCAAGAAGACACTGATTTCTGAACTATCAGGAAAGCCAACCTCCCAAGCAGAAGTAAGGGATT TATATCCAGGTCTGGGAGAAGATTTTAGTGGTGACTCCAGTGAATATCCAACAGTGTCTTCTACAACAAT GAAAGAGGAAACAGTGGGGATGGGAGGCTCTGAGAATGAACGAGTTAAGGATACACAGACTTTATCATCT ATACCACCTACCTCAGACAACATCAACCCAGTGCCTGACTCAAAAGGATTCGGCAGTACTGTGGCCAGCA CCACAGCCTTCCCTTGGGAAGAGTTCATGACCTCAGCTGAGGGCTCAGGTGAGGAGCTGTCCTCAGTCAG GAGCTCTGTTAGTCTAGTGCTTCCACTAGGTGTGGACATTCTTCCTACTACAGAGTCTCCATATTTTGAT CAAGAGTTTGAAGAAGCGGCTGCAGTGACAGAAGCAGGTAAACAATCTGCTTTGCCAATAGCAGTGAGTG GAAATACTGTAGATCTGACAGAAAACAGGGACATAGAGGTAAACAGCACAATGTCAGTAGACCTTCCCCA GACAATGGAGCCAGCCAAATTATGGTCTAAACCAGAAGTCAATCCTGAAAAACAAGAGATTGGAAGCGAA ACAGTGACACAGGATAAAGCTCAGGGACAAAAGTCTTTTGAATCGCTCCACAGTTCTCTTGCACCAGAAC AAACAACTTTGGAAAGCCAATCACTTATTGAAACTGAAGTCCAAACTTCATATTACTCTATGTTAACAAC AATGAAAACATACAATACTAATGAGGAAGTGGAGGAGGAAGGCACGTCCATAGCTCATATGTCTACTCCA GGCCCGGGAATCAAAGGCTTGGAATCATATCCTACCCATCCTGAAGCTACTGGGAAGTCATACTCTTTCT CAGCTTCTGCCTTAGTGACTGAATCTGGACCAGCTAGAAGTGTAGTCATGGATTCCTCAACTCAAGAGGA AGAAAGTATAAAACTCTTCCAAAAGGACATGATACTAACACATAAAGAGTCAAATTCTGATCTCTCGTTC TCTGGCCTGGGATCAGGAGAGGCTTTGCCTCCTCTCCCTACAACATCAGTGAGTTTGACTGACATGGGAA AGATCAATAGCACATTGTATCCTGAGACATCTCACATGGAAAGCTTAGGAACAAGCATCTTAGGAGATAA CCATGAAAGAATGAAAAATGTGTCAAATGAAGTTAGAACCCTTATTTCCGAAACAGGCAGCATTTCTCAA GACAGTACGGAGGCACCCAATACAACTTTGAGTGATACCAGAACAGAAGAATCTACAACTTCACCTCTTC CTTTCATGAAGCTCATGGACACGGAACATTCTCCAAAGCAGACCCTCAGGTGGGAAGAGGAAATCCAGAC CCATAGGCCACAAACTATGACTGGACAGATTACCAATGACAATTCTTCAGTGTCAGAAGCTGAAGCAGCA GCAACCTCTGCCCCTGCTTTTCTGCCTGAAACCTACAGTGTTGAAATGACCAAAGCCTTTGCTACATCAC TGTCACAAACATCTGACTTGTTTGATGCAAATTCTGGAGAAGGATCTGGAGAAGTGGATGGTCTTGACTT AGTGTACACTTCTAGAACTACTCAGGCAAGCAGTCAAGGAGACAGCATGTTTGCCTCTCATGGATTCATT GAAAAGCATCCAGAGGTGTCAAGGACCGAGACTGGAGCTACTGATGGATCTCCAACAGCTTCAGCGATGT TCCTGCATCAGTCAGAGTACAATGAGAGTTCCCTGTATCCAACCAGCACACTGCCAAGCACAGTGACATA TGAGAGTCCCTCAGAAGGTATTGCAGATGGTTTGCAAGACCATATCAGATTCGAAGTTTCTACCTTGAAA CCTAGCAGAAGGAAAGCCACTGAGAGCGTTATTATAGATTTAGACAAAGAGGATAGTAAGGACTTAGGGT TGGCAATTACTGAGAGTGCTATTGTTGAAATTCTCCCTGAGCTGACATCAGATAGAAATATTATTATAGA CATTGATCACACTAAACCTGTATATGAGTACATCCCTGGAATACAAACAGACCTAGATTCAGACATACCA TTGGGATCACATGGCAGTAGTGAAGAAAGCCTTGAAGTTCAAGAGAAGTATGAAGCAACTATTAACCTGT CTCCAACTGAGGAAGCATTTGACGGCTCTGGTGATGCACTTCCTGCTGGTCACACTCAAGCGATATACAA TGAGTCTGTGACTCCCAGTGATGGAAAGCAGCCAGAGGACATAAGCTTTAGTTTTGCAACTGGAATCCCA GTTTCTAGCACAGAGACAGAATTAAATACTTTCTTCCCTACAGTGTCAACCCTGCACATTCCTAGCAAAC TAACCACCGCTAGTCCAGAGATTGATAAACCCAACATTGAAGCAATATCACTGGATGATATATTTGAATC AAGCACCTTGTCTGATGGCCAAGCTATTGCCGATCAGAGTGAAGTAATATCAACACTAGGCCATTTGGAA AAGACACAGGAAGAGTATGAAGAAAAGAAATATGGAGGTCCTTCTTTTCAGCCAGAATTCTTTTCAGGAG TGGGTGAAGTATTCACAGATGCCCCTGCTTATGTAAGTATTGGTAGGACATACTCTGTAGCTCAGCCTTT AACAGAATTCCCCAATGTGGTAGGACAATCTGACTCCACACATTACACTGAAGCAACATCTGCAGTTTCC TCTGTGACAGAGCTGTCTCCTCAGACACCATCTTCTCCCTCTCCTGTCTACATAGACAGTGGAGTCTCTG AATTCACAGAGGTCCCCCACAAAAGTGCTCAGCCAGCTCCTACTGCCGCTTCATCACAAAAGTTGATAGA GGGTTCTTTTAAGGAAGTGCGTGCTAATATTGAAGCTACCATCAAGTCGCTGGGTGAAAATGATCACGGA ACAGAGTCTCCATCCATGTCTCCTAGCCCTGCTTTAGACATTTCAGAAGACGACAGTAAACCTAAGTTAC TAGAAGACCTGGAAACTTCTCCCACAAAAACCGAGACTTCCCAAGATTCCCCAAACAAAGCAAATGATCA AATTCCAGGAAAAACAGCTGGAATCCTTGCTGGTATCAAAACAACAGAGAGTGGACCAGTCGTTACAGCT GCAGATGACATGGAGCTAGGAGATGCCACACAGCGGCCACATTCTGCTTCTGCTCCTGCAGCTTTCAGGG TTGAGACAAGTATGGTACCTCAGCCCATCCCACAGGAGCCTGAGAGGCCAACCTTTCCTTCCCTGGAAAT TAACCATGAAACACACACATCATTATTTGAAGAATCTATATTGGCAACATCTGAAAAACAAGTGTCCCAA AGAATTCTTGATTACAGTAACCAGGCAACAGTCAGTACTCTGGATCTAAATACTGAACACTCAATACCAC CATTTTCCATTCTGGACAATTCTAACGAAACTGCTTTCCTGATTGGCATTAGTGAAGAGACCGTGGAAGG CACAGCAGTTTATCTACCAGGACCTGATCTCTGCAAAACAAACCCATGCCTCAATGGAGGCACCTGCTAT CCTACTGAGACTTCCTATGTGTGCACCTGTGCACCTGGCTACAGTGGAGACCAGTGTGAACTGGATTTTG ATGAATGTCACTCTAACCCTTGTCGGAATGGAGCCACCTGTGTGGACGGTCTGAATACATTTAGATGCCT CTGCCTTCCGAGTTATGTCGGTGCACTCTGCGAACAAGACACTGAGACATGCGACTATGGCTGGCACAAA TTCCAAGGGCAATGCTACAAGTACTTTGCTCATCGCCGTACATGGGATGCTGCTGAAAGGGAGTGTCGCC TGCAGGGTGCCCACCTCACAAGCATCCTTTCTCATGAGGAACAAATGTTTGTGAATCGTGTGGGCCATGA TTACCAGTGGATTGGCCTCAATGACAAGATGTTTGAACATGACTTCCGCTGGACTGACGGCAGCGCACTG CAATATGAGAACTGGAGACCCAACCAGCCAGACAGCTTCTTTTCTGCTGGAGAAGACTGCGTTGTGATCA TTTGGCATGAGAATGGCCAGTGGAATGACGTCCCCTGCAACTACCACCTCACCTACACCTGCAAGAAGGG AACAGTTGCTTGCGGCCAACCCCCTGTTGTAGAAAATGCCAAGACCTTTGGAAAGATGAAACCACGTTAT GAAATCAACTCCTTGATTAGATACCACTGCAAAGATGGTTTCATTCAGCGTCACCTTCCAACTATCCGGT GCCTAGGAAATGGGAGATGGGCAATGCCTAAAATAACCTGCATGAACCCATCTGCATACCAAAGGACTTA TTCTAAGAAATACTTAAAAAATTCCTCATCAGTCAAGGACAATTCTATAAATACGTCAAAACATGAGCAT CGCTGGAGCCGGAGGTGGCAGGAAACGAGGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217768 representing NM_053663 Red=Cloning site Green=Tags(s) MLINMNGILWMCSTLLLTHALHKAKMEENPPVKGSLSGKVILPCHFSTLPTLPPDYNTSEFLRIKWSKIE VDKNGKDIKETTVLVAQDGNIKIGQDYKGRVSVPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQ NTMSLAVDGVVFHYRAATSRYTLNFESAQQACLDIGAVIATPEQLFAAYEDGFEQCDAGWLSDQTVRYPI RAPREGCYGDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECANRDARLATV GELHAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCFPLPDSRFDAYCFKRRM SDMVVNGHPIDSESKEEEPCSEETDPLHDLFAEILPELPDSFEIDIYHSEEDEDGEEDCVNATDVTTTPS VQYITGKPHVTTVPKNPEAAEARRGLYESVAPSQNFSNTSATDTHQFIPAETELSTTMQFTKSKEATELL EITWKPETYPETPEHFSSGEPDVFPTLPSHDGKTTKWSEFITESNPNTENPEHKQPKPIPLFPEEFSGEG AIDQASQQTIFSTATEVALGKETDQSPTISTSSIRSGSVSVHALEEDPIALTGISQTDESMSTVESWVEM TPSQTVEFSGSSSAPTIEGSGEVEEYTNKIFNTVTDLPQREPTDTLIPLDMSNIMITDHHIYTPATTAPL DSQLPSTDARPTQFGIQTTTSEWVSSTSFEGRKTEEDKERDTNAAHTGEVQPATERSDRLLLTSELESSN VAASSPLDTWEGFVPETTSTVSEKEMANTTPVFTETSDVANLETQSFEHSSSSQPRVQEELTTLSGKPPL IFMDLGSGDASTDMEFITASSFTLDLESDTKVKKELPSTLSPSVETSSSSEPIGLAPSTVLDIEIVEVMN QTSKKTLISELSGKPTSQAEVRDLYPGLGEDFSGDSSEYPTVSSTTMKEETVGMGGSENERVKDTQTLSS IPPTSDNINPVPDSKGFGSTVASTTAFPWEEFMTSAEGSGEELSSVRSSVSLVLPLGVDILPTTESPYFD QEFEEAAAVTEAGKQSALPIAVSGNTVDLTENRDIEVNSTMSVDLPQTMEPAKLWSKPEVNPEKQEIGSE TVTQDKAQGQKSFESLHSSLAPEQTTLESQSLIETEVQTSYYSMLTTMKTYNTNEEVEEEGTSIAHMSTP GPGIKGLESYPTHPEATGKSYSFSASALVTESGPARSVVMDSSTQEEESIKLFQKDMILTHKESNSDLSF SGLGSGEALPPLPTTSVSLTDMGKINSTLYPETSHMESLGTSILGDNHERMKNVSNEVRTLISETGSISQ DSTEAPNTTLSDTRTEESTTSPLPFMKLMDTEHSPKQTLRWEEEIQTHRPQTMTGQITNDNSSVSEAEAA ATSAPAFLPETYSVEMTKAFATSLSQTSDLFDANSGEGSGEVDGLDLVYTSRTTQASSQGDSMFASHGFI EKHPEVSRTETGATDGSPTASAMFLHQSEYNESSLYPTSTLPSTVTYESPSEGIADGLQDHIRFEVSTLK PSRRKATESVIIDLDKEDSKDLGLAITESAIVEILPELTSDRNIIIDIDHTKPVYEYIPGIQTDLDSDIP LGSHGSSEESLEVQEKYEATINLSPTEEAFDGSGDALPAGHTQAIYNESVTPSDGKQPEDISFSFATGIP VSSTETELNTFFPTVSTLHIPSKLTTASPEIDKPNIEAISLDDIFESSTLSDGQAIADQSEVISTLGHLE KTQEEYEEKKYGGPSFQPEFFSGVGEVFTDAPAYVSIGRTYSVAQPLTEFPNVVGQSDSTHYTEATSAVS SVTELSPQTPSSPSPVYIDSGVSEFTEVPHKSAQPAPTAASSQKLIEGSFKEVRANIEATIKSLGENDHG TESPSMSPSPALDISEDDSKPKLLEDLETSPTKTETSQDSPNKANDQIPGKTAGILAGIKTTESGPVVTA ADDMELGDATQRPHSASAPAAFRVETSMVPQPIPQEPERPTFPSLEINHETHTSLFEESILATSEKQVSQ RILDYSNQATVSTLDLNTEHSIPPFSILDNSNETAFLIGISEETVEGTAVYLPGPDLCKTNPCLNGGTCY PTETSYVCTCAPGYSGDQCELDFDECHSNPCRNGATCVDGLNTFRCLCLPSYVGALCEQDTETCDYGWHK FQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSAL QYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRY EINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCMNPSAYQRTYSKKYLKNSSSVKDNSINTSKHEH RWSRRWQETRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053663
ORF Size	7173 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053663.1, NP_446115.1
RefSeq Size	9470 bp
RefSeq ORF	7176 bp
Locus ID	114122
MW	261.8 kDa
Gene Summary	extracellular matrix protein secreted by many cell types; part of the large chondroitin sulfate proteoglycan (CSPG) family [RGD, Feb 2006]

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