Vcan (NM_053663) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cspg2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217768 representing NM_053663 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAAATATGAACGGCATCCTATGGATGTGCTCAACCTTACTGTTAACGCATGCACTGCATAAAG CCAAAATGGAAGAAAACCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGATCCTACCTTGTCATTTTTC AACCTTGCCCACCTTACCACCCGATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAAATAGAA GTGGACAAAAATGGAAAAGACATAAAGGAGACTACTGTCCTGGTGGCCCAAGACGGGAACATCAAGATTG GTCAGGACTACAAGGGGCGGGTATCAGTGCCTACGCATCCCGATGACGTAGGCGATGCCTCTCTCACCAT GGTCAAACTCCGTGCTAGTGACGCAGGTGTCTACCGCTGTGATGTCATGTATGGCATTGAAGACACTCAG AACACGATGTCGCTGGCCGTGGACGGTGTCGTGTTTCACTACAGGGCAGCGACCAGCAGATACACTCTGA ACTTCGAGTCTGCTCAACAGGCTTGTTTGGACATCGGGGCGGTCATAGCAACCCCAGAGCAGCTGTTCGC TGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGACCAAACTGTCAGATATCCCATA CGGGCTCCCCGAGAGGGCTGTTATGGAGACATGATGGGGAAGGAAGGGGTCCGGACCTATGGATTCCGCT CTCCCCAGGAAACCTATGATGTGTATTGCTATGTGGATCATCTGGACGGCGATGTGTTCCACATCACTGC TCCCAGTAAATTCACCTTCGAGGAGGCCGAAGCAGAGTGTGCAAACCGGGATGCCAGGCTGGCGACTGTT GGGGAACTTCACGCAGCTTGGAGGAACGGCTTTGACCAGTGCGATTACGGCTGGCTATCGGATGCCAGCG TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACGTCGAATG AGTGATATGGTTGTAAATGGTCATCCAATAGATTCAGAATCTAAAGAAGAGGAACCTTGCAGTGAAGAAA CAGATCCACTGCATGATCTGTTTGCTGAAATTTTACCCGAGTTACCAGATTCCTTCGAAATAGACATATA TCACAGTGAGGAAGATGAAGATGGAGAGGAGGACTGTGTAAATGCAACTGATGTAACAACCACTCCGTCA GTGCAGTACATCACTGGGAAGCCACATGTCACTACAGTGCCCAAAAACCCAGAAGCTGCAGAAGCGAGGC GTGGCCTGTATGAAAGTGTTGCGCCTTCTCAGAATTTCTCAAATACTTCCGCAACTGACACCCATCAGTT TATACCGGCCGAAACAGAATTGTCAACTACCATGCAATTTACCAAATCCAAAGAAGCCACTGAATTGTTA GAAATCACATGGAAACCCGAGACCTACCCTGAAACACCAGAACACTTTTCAAGTGGTGAGCCTGATGTTT TCCCTACACTGCCATCCCATGATGGTAAAACCACCAAATGGTCAGAGTTCATCACAGAGAGCAATCCAAA CACAGAAAATCCAGAACACAAACAACCTAAGCCTATACCTCTGTTTCCTGAAGAGTTTTCAGGAGAGGGT GCCATTGACCAAGCATCCCAGCAAACAATCTTTTCCACGGCTACAGAAGTAGCACTTGGTAAAGAGACAG ACCAAAGTCCTACCATATCTACCTCTAGCATACGTTCAGGTTCTGTGTCAGTACATGCCTTGGAGGAAGA TCCAATTGCTCTAACAGGAATCTCACAGACTGATGAATCCATGTCTACTGTGGAAAGCTGGGTAGAGATG ACCCCTAGCCAAACTGTAGAGTTCTCTGGGAGTTCTTCAGCTCCTACTATTGAAGGGTCTGGGGAAGTAG AAGAATATACAAATAAAATTTTCAACACGGTAACTGATTTGCCACAGAGAGAGCCTACAGATACGCTCAT CCCCTTGGATATGAGCAACATCATGATCACAGACCACCACATTTATACTCCTGCAACCACCGCTCCTTTG GATTCACAGCTACCTTCTACAGATGCACGGCCTACACAATTTGGAATTCAAACCACCACCTCTGAGTGGG TTTCCAGTACATCTTTTGAAGGAAGGAAAACAGAGGAAGACAAAGAAAGAGATACAAATGCAGCCCACAC AGGGGAAGTCCAACCAGCGACAGAGAGGTCAGATCGCTTACTCTTAACTTCAGAATTAGAAAGTTCAAAT GTGGCTGCATCTAGTCCTTTGGACACCTGGGAAGGTTTTGTGCCAGAGACAACATCAACAGTGTCCGAAA AGGAAATGGCAAACACAACTCCTGTTTTTACAGAAACAAGTGATGTAGCCAATTTGGAGACACAGTCTTT TGAGCATAGTAGTAGCAGTCAGCCCAGGGTACAGGAAGAGCTGACCACTCTCTCAGGAAAGCCCCCCTTG ATTTTTATGGACCTGGGTTCAGGAGATGCTAGTACTGATATGGAATTCATCACTGCTTCTTCATTTACAT TAGATTTGGAGTCCGATACCAAAGTCAAAAAAGAATTACCCAGTACTCTGTCTCCCAGTGTGGAGACTTC ATCCTCCTCTGAGCCAATAGGATTAGCTCCGAGTACTGTATTGGACATAGAAATTGTGGAAGTTATGAAT CAGACATCCAAGAAGACACTGATTTCTGAACTATCAGGAAAGCCAACCTCCCAAGCAGAAGTAAGGGATT TATATCCAGGTCTGGGAGAAGATTTTAGTGGTGACTCCAGTGAATATCCAACAGTGTCTTCTACAACAAT GAAAGAGGAAACAGTGGGGATGGGAGGCTCTGAGAATGAACGAGTTAAGGATACACAGACTTTATCATCT ATACCACCTACCTCAGACAACATCAACCCAGTGCCTGACTCAAAAGGATTCGGCAGTACTGTGGCCAGCA CCACAGCCTTCCCTTGGGAAGAGTTCATGACCTCAGCTGAGGGCTCAGGTGAGGAGCTGTCCTCAGTCAG GAGCTCTGTTAGTCTAGTGCTTCCACTAGGTGTGGACATTCTTCCTACTACAGAGTCTCCATATTTTGAT CAAGAGTTTGAAGAAGCGGCTGCAGTGACAGAAGCAGGTAAACAATCTGCTTTGCCAATAGCAGTGAGTG GAAATACTGTAGATCTGACAGAAAACAGGGACATAGAGGTAAACAGCACAATGTCAGTAGACCTTCCCCA GACAATGGAGCCAGCCAAATTATGGTCTAAACCAGAAGTCAATCCTGAAAAACAAGAGATTGGAAGCGAA ACAGTGACACAGGATAAAGCTCAGGGACAAAAGTCTTTTGAATCGCTCCACAGTTCTCTTGCACCAGAAC AAACAACTTTGGAAAGCCAATCACTTATTGAAACTGAAGTCCAAACTTCATATTACTCTATGTTAACAAC AATGAAAACATACAATACTAATGAGGAAGTGGAGGAGGAAGGCACGTCCATAGCTCATATGTCTACTCCA GGCCCGGGAATCAAAGGCTTGGAATCATATCCTACCCATCCTGAAGCTACTGGGAAGTCATACTCTTTCT CAGCTTCTGCCTTAGTGACTGAATCTGGACCAGCTAGAAGTGTAGTCATGGATTCCTCAACTCAAGAGGA AGAAAGTATAAAACTCTTCCAAAAGGACATGATACTAACACATAAAGAGTCAAATTCTGATCTCTCGTTC TCTGGCCTGGGATCAGGAGAGGCTTTGCCTCCTCTCCCTACAACATCAGTGAGTTTGACTGACATGGGAA AGATCAATAGCACATTGTATCCTGAGACATCTCACATGGAAAGCTTAGGAACAAGCATCTTAGGAGATAA CCATGAAAGAATGAAAAATGTGTCAAATGAAGTTAGAACCCTTATTTCCGAAACAGGCAGCATTTCTCAA GACAGTACGGAGGCACCCAATACAACTTTGAGTGATACCAGAACAGAAGAATCTACAACTTCACCTCTTC CTTTCATGAAGCTCATGGACACGGAACATTCTCCAAAGCAGACCCTCAGGTGGGAAGAGGAAATCCAGAC CCATAGGCCACAAACTATGACTGGACAGATTACCAATGACAATTCTTCAGTGTCAGAAGCTGAAGCAGCA GCAACCTCTGCCCCTGCTTTTCTGCCTGAAACCTACAGTGTTGAAATGACCAAAGCCTTTGCTACATCAC TGTCACAAACATCTGACTTGTTTGATGCAAATTCTGGAGAAGGATCTGGAGAAGTGGATGGTCTTGACTT AGTGTACACTTCTAGAACTACTCAGGCAAGCAGTCAAGGAGACAGCATGTTTGCCTCTCATGGATTCATT GAAAAGCATCCAGAGGTGTCAAGGACCGAGACTGGAGCTACTGATGGATCTCCAACAGCTTCAGCGATGT TCCTGCATCAGTCAGAGTACAATGAGAGTTCCCTGTATCCAACCAGCACACTGCCAAGCACAGTGACATA TGAGAGTCCCTCAGAAGGTATTGCAGATGGTTTGCAAGACCATATCAGATTCGAAGTTTCTACCTTGAAA CCTAGCAGAAGGAAAGCCACTGAGAGCGTTATTATAGATTTAGACAAAGAGGATAGTAAGGACTTAGGGT TGGCAATTACTGAGAGTGCTATTGTTGAAATTCTCCCTGAGCTGACATCAGATAGAAATATTATTATAGA CATTGATCACACTAAACCTGTATATGAGTACATCCCTGGAATACAAACAGACCTAGATTCAGACATACCA TTGGGATCACATGGCAGTAGTGAAGAAAGCCTTGAAGTTCAAGAGAAGTATGAAGCAACTATTAACCTGT CTCCAACTGAGGAAGCATTTGACGGCTCTGGTGATGCACTTCCTGCTGGTCACACTCAAGCGATATACAA TGAGTCTGTGACTCCCAGTGATGGAAAGCAGCCAGAGGACATAAGCTTTAGTTTTGCAACTGGAATCCCA GTTTCTAGCACAGAGACAGAATTAAATACTTTCTTCCCTACAGTGTCAACCCTGCACATTCCTAGCAAAC TAACCACCGCTAGTCCAGAGATTGATAAACCCAACATTGAAGCAATATCACTGGATGATATATTTGAATC AAGCACCTTGTCTGATGGCCAAGCTATTGCCGATCAGAGTGAAGTAATATCAACACTAGGCCATTTGGAA AAGACACAGGAAGAGTATGAAGAAAAGAAATATGGAGGTCCTTCTTTTCAGCCAGAATTCTTTTCAGGAG TGGGTGAAGTATTCACAGATGCCCCTGCTTATGTAAGTATTGGTAGGACATACTCTGTAGCTCAGCCTTT AACAGAATTCCCCAATGTGGTAGGACAATCTGACTCCACACATTACACTGAAGCAACATCTGCAGTTTCC TCTGTGACAGAGCTGTCTCCTCAGACACCATCTTCTCCCTCTCCTGTCTACATAGACAGTGGAGTCTCTG AATTCACAGAGGTCCCCCACAAAAGTGCTCAGCCAGCTCCTACTGCCGCTTCATCACAAAAGTTGATAGA GGGTTCTTTTAAGGAAGTGCGTGCTAATATTGAAGCTACCATCAAGTCGCTGGGTGAAAATGATCACGGA ACAGAGTCTCCATCCATGTCTCCTAGCCCTGCTTTAGACATTTCAGAAGACGACAGTAAACCTAAGTTAC TAGAAGACCTGGAAACTTCTCCCACAAAAACCGAGACTTCCCAAGATTCCCCAAACAAAGCAAATGATCA AATTCCAGGAAAAACAGCTGGAATCCTTGCTGGTATCAAAACAACAGAGAGTGGACCAGTCGTTACAGCT GCAGATGACATGGAGCTAGGAGATGCCACACAGCGGCCACATTCTGCTTCTGCTCCTGCAGCTTTCAGGG TTGAGACAAGTATGGTACCTCAGCCCATCCCACAGGAGCCTGAGAGGCCAACCTTTCCTTCCCTGGAAAT TAACCATGAAACACACACATCATTATTTGAAGAATCTATATTGGCAACATCTGAAAAACAAGTGTCCCAA AGAATTCTTGATTACAGTAACCAGGCAACAGTCAGTACTCTGGATCTAAATACTGAACACTCAATACCAC CATTTTCCATTCTGGACAATTCTAACGAAACTGCTTTCCTGATTGGCATTAGTGAAGAGACCGTGGAAGG CACAGCAGTTTATCTACCAGGACCTGATCTCTGCAAAACAAACCCATGCCTCAATGGAGGCACCTGCTAT CCTACTGAGACTTCCTATGTGTGCACCTGTGCACCTGGCTACAGTGGAGACCAGTGTGAACTGGATTTTG ATGAATGTCACTCTAACCCTTGTCGGAATGGAGCCACCTGTGTGGACGGTCTGAATACATTTAGATGCCT CTGCCTTCCGAGTTATGTCGGTGCACTCTGCGAACAAGACACTGAGACATGCGACTATGGCTGGCACAAA TTCCAAGGGCAATGCTACAAGTACTTTGCTCATCGCCGTACATGGGATGCTGCTGAAAGGGAGTGTCGCC TGCAGGGTGCCCACCTCACAAGCATCCTTTCTCATGAGGAACAAATGTTTGTGAATCGTGTGGGCCATGA TTACCAGTGGATTGGCCTCAATGACAAGATGTTTGAACATGACTTCCGCTGGACTGACGGCAGCGCACTG CAATATGAGAACTGGAGACCCAACCAGCCAGACAGCTTCTTTTCTGCTGGAGAAGACTGCGTTGTGATCA TTTGGCATGAGAATGGCCAGTGGAATGACGTCCCCTGCAACTACCACCTCACCTACACCTGCAAGAAGGG AACAGTTGCTTGCGGCCAACCCCCTGTTGTAGAAAATGCCAAGACCTTTGGAAAGATGAAACCACGTTAT GAAATCAACTCCTTGATTAGATACCACTGCAAAGATGGTTTCATTCAGCGTCACCTTCCAACTATCCGGT GCCTAGGAAATGGGAGATGGGCAATGCCTAAAATAACCTGCATGAACCCATCTGCATACCAAAGGACTTA TTCTAAGAAATACTTAAAAAATTCCTCATCAGTCAAGGACAATTCTATAAATACGTCAAAACATGAGCAT CGCTGGAGCCGGAGGTGGCAGGAAACGAGGCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053663
Insert Size	7176 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053663.1, NP_446115.1
RefSeq Size	9470 bp
RefSeq ORF	7176 bp
Locus ID	114122
Gene Summary	extracellular matrix protein secreted by many cell types; part of the large chondroitin sulfate proteoglycan (CSPG) family [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2, also referred to as V1), compared to isoform 1.

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