Sec16a (NM_001276417) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217765 representing NM_001276417 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCACCACCTCAGGCAGTCCCGTCTGGTGTGGCTGGCCCACCCCCAGCTGGGAATCCTCGAAGCT CGTTCTGGGCCAACAGCCCTTACAGGAAGCCAGCCAATAATAATGCACCAGTGGCTCCCATAACACGCCC ACTGCAGCCAGTAACGGATCCGTTTGCTTTTAACAGACAGACTCTTCAAAACACACCAGTGGGCAGTTCA TCCAAAAGCAGCCCACCTAATTTGCCTGGCCCAGCCCTCCCTGTTTTTTCTCAGTGGCCTGGTTTGCCTG TGCCTCCCACAAATGCTGGGGATAGCTCCACAGGACTTCATGAGCCTCTGCCAGGAACTCTGTCACAGCC TAGAGCAGACCCCAGTCTATTTCCTCCTGCATCTACCCCTTCTTCATTGCCTGGGCTGGAGGTGAACAGG AGTGCTGAGGTTGATTCCAGCTCAGGACCTGAAGTCCAGATGCTGCCACATCCACCTCACTACATTCCAG GAGTGGGTCCTGAGCAGCCTCACGGAGGCCATCTGAATGACAGTGGGGCTGGGCCTGACCAATCCATGAA TAGGCACGCCCCACATGATGGTGCTGTGGCCCATGCAGCATCTCCTTTCTTCCCTCAGCCCCAGATGCCA GGTCAGTGGGGGCCTGTGCATGGAGGCCCTCCGCCCTCCTATCATCATCACTCACCCTGCCTGGAGGGCC CTGTTCAGGACATGGGGCCCCAGGCTGCCAGCTTGCCCCATTTCCCCTCTCCATCTAGTCTACACGAGGG ACCTGGCCATGAGTCCCGTGTCCCACAAACGTTTACTCCAGCATCCTTGGCCAGTGGTGAAGGAAATGAG ATAGTCCACCAGCAAAGTAAAAATTACCCATCGAGTAGCTTCCCCCCTAAACACAGTTTTGAGCAGAATT CCAGAGTTGGGAATATGTGGGCAAGCGCGGAGTTCAGGCAGAATCCAGGAGTTAATAAAGAGCATCTGCT AGACCCTGCCCATGTGAATCCCTTCACTCAGGGAAACAGCCCTGAGAACCAGGTGCACCATCCCCCAGTG GCCGCAACTAACCATGCCCTGCAAGAAGCAGCCTCCGGAGCGCTCTCAATGTTTTTTCAAGGGGAGGAGA CAGAGAACGAGGAAAATCTCTCATCTGAAAAAGCAGGCCTTGATGACAGATTAGACTCGGGTAGCTTCTC TTCTACCTCCAGACTGGGCCATCCACCACTCCCTGGAGCTGGTGGTGTCTACCAGGCCGCTCAGGAAGGA GATGTGCAACCTTATTTTTCTCAGTTTGTGGGCATCCGTCATGACAAACAGACCACTATGACTCCTGCTA ATGATGCGTGGGGTGATATTCCGGGTGCTGGGACCCACTGTGCTAGTGGGCCACAGTGTGAGAATGTAGA GAACCTAGAATTTGTTCAGAATCAAGAAGTTTTGCCACGTGAGACCGTGAATGTGGATCCCTTTTCCCCG AGTGATCAGTTTCGATATGGGCCCCTTCCTGGGCCAGCTGCCTCCAGGCCTGTTACTTCGGGCCTCACTA GAGGAGGAGGGCTAAATCTTGAGGCCCCTGACATGCCACTGCACCCTACACGACCTGACAGTGTGTCATC CAGCTACAGCAACCATAGTCACAGAAGTCCTCCTGGGTCAGCCAGACCCCAAGAATTGGTGGGCACATTC ATTCAGCAAGAAGTTGGAAAACTCGAAGATGATACGTCAGGGAGTTTTTTTAAGCAAATTGATTCTTCTC CGGTTGGAGGTGAAACAGATGAGATGACCAGGAGTCAAAATTACTGCAGCAGCCTGTCCCAGCCTTCAAC TCCAAGCCCTCCAAAACCTACGGGAGTCTTCCAGACAAGTGCAAATAGTTCTTTTGAGCCAGTAAAATCC CACTTGGTTGGAGTGAAACCAGTTGAAGCAGATCGTGCCAACATGGTGGTTGAAGTGAGAGGGACCCAGA ACTGCCAGAAGCACGGGGCCGCTGTTCTACCACCTGATGCCACCCCAGGAAACCTGGAACAGCCACCAGA CAACATGGAGACCCTGTGTGCACCTCAGGCCTGCCCTTTGCCTCTTAGCACCACTGGAGAGGCTGGGCAA CTGGTTTCACACACAGCAGGGCCACCCTTGGATACTGTGCGCCCCATGCCTGACAAGAGGTCCTCAGCCA GGGCCCAGGGGCCTGTGAAATGTGAGAGCCCAGCCACAACTCTGTGGGCACAAAACGAACTGCCAGATTT TGGGGGCAATGTCCTTCTAGCCCCTGCTGCCCCTGCACTTTATGTTCCTGTAAAACCAAAACCTTCAGAG GTTGTTCATCATCCAGAAAAGGGCATGTCTGGACAGCAGTCATGGAAATCAGGGTCTGTGCCCCCTTTGC AGAACCAAGACCCTCCTGGTGCTTCTGAGAACCTTGAGAACCCTCCCAAGGTAGGAGAAGAGGAGGCCCT TCCATTGCAGGCAAGTTCTGGTTATGCGAGTTTGCTGTCCTCACCACCCACTGAATCTTTACACAATCAA CCTGTCCTGATTGCCCAGCCTGATCAAAGCTATAATTTGGCTCAGCCCATTAATTTTTCTGTGTCCTTAC CGAATCCTAATGAGAAGAATCAGTGCTGGGGAGATGCTGTGGTGGGGGAGAAATCCATGGTGAGCACCCG GGCTCTTGGTGCTGACCATGAAGAACATGCTGCCTTGTCTGCAATTCCAGCCAGTACTGTCACTGGCTCG TCTTTGCCTAACAGTGTCACCCAGAACTGTGCCCCTCGAGGTTCTGGGTCTTCTGAAATGATTGCTAGCC AGCCTGCCAGTTGGTTGGTTCAACAGCTGTCCCCTCAGATTCCTCAAAGTCAGCACCCCAATGCAGAGAA GGGTCCTTCTGAGTTTGTTAATAGCCCTGCTAATGGAAGTGTGATGCTAGCTCCACCTGCAAACAGTGCT GTGATACCTAACAGTAATAAGGGGTATCACTCTAGAAACCCGGAAGAAGCCATCGGTGCCCTAGACTTCA CATTGAGTAGAACTTTGGAAAATCCTGTGAGAATGTGTAGCCCATCTCATTCAAACAGCCCAGCTTCTCA GCAGCCTCTTGCCAATCATCCCAGACAATCTGGGCCTGGGCTGCATAACCAGGATCATTTCTACCAGCAG GTAACAAAAGATGCTCAGGATCACCGTAGCCTAGAAAGAGGCCAGTCAGAGCTGGTGCCTCCTCCGCCAC AGAATTCTCCCCAACAAGTACCCCAAGCTGCGTGTCCAGAGCCTTCAAATCCAGAAAGCCCACCCACACA AGGACAGTCTGAAGGCTTGGTCCAGCCATCAGCAAGTCCAGCTCCAGTCAACACAGGCCAGCAGCCTCAG CCACCGCAGTCCTCCAGTGCATCGGTTACATCTACCAACTCCAACCAGGCAGCTGTGCGGTCAGAACAGC CATGGCTACACCCACCTCCCACTACTTTTGGCCCAGCACCTCAAGACTTGGCATCCTACTACTATTATAG ACCCCCGTATGAAGCCTACCAGTCTCCGTACCCTTCACCGTACCCATCAGATCCTGGAGCAGCCTCTTTA TACTACCAAGATATCTATGGCCTGTATGAGCCTCGTTACAGACCCTATGACAGCTCGGCATCTGCTTATG CCGAGAACCACCGCTACTCTGAACCTGAGCGGCCCAGCTCCCGTGCAAGTCACTATTCTGACCAGCTCAC TCCCAGGCAAGGATATCCTGAAGGATACTACAATTCTAAGAGTGGATGGAGCAGTCACAGTGACTACTAC GCAAATTACTACTCTGGCCAGTATGATTATGGAGACCCAGGCCGCTGGGACCGTTACTATGGGTCTCGAT TTAGGGACCCTCGCACCTGGGACCGGAGGTACTGGTATGACTCTGACCATGACCCATACAGGAAGGACAA CTATGCTTACAGTGACAGGCCTGAGAAATGTGATGATCACTGGAGGTATGACCCTCGCTTCACTGGGAGC TTCGATGATGACACTGAGCTCCACAGGGACCCCTATGGAGAAGAAGTAGACAGACGCAGCATCCACAGCG AGCACTCAGCCCGGAGCCTGCGCAGTACTCACAGCCTGCCCAGCCGCCGCAGCAGCCTCAGCTCCCATTC ACACCAGAGTCAGATTTACAGAAGCCACCATGTGACTGGCGGTTCCTTTGAGGCCCCCCATGCCCCAGGC TCATTTCATGGTGATTATGCCTATGGTACCTATGCCAGCAATTTCAGCGGTGCCCATGGTTTTCCAGAGT ACAGCTACCCTGCAGACACCTCCTGGCCTGCTGCGGAGCAAGTTCCATCAAGACCGACCTCTCCTGAGAA GTTTACAGTGCCTCACGTCTGTGCCAGGTTTGGTCCTGGTGGTCAGCTCCTCAAAGTGATACCCAACCTG CCTTCAGAAGGACAGCCCGCATTGGTGGAGATCCACAGCTTAGAGACCTTGCTGCAGCACACACCTGAGC AGGAAGAAATGCGCTCCTTCCCAGGACCTCTCGGCAAAGATGACACTCACAAAGTAGATGTTATTAACTT TGCACAAAACAAAGCGACAAAATGTTTGCAGAATGAAAGTTTAATTGACAAAGAGTCTGCAAGTCTTCTT TGGAACTTCATTATTCTGTTATGCAGACAGAATGGGACCGTCGTGGGAACAGACATCGCAGAGCTCTTGT TACGGGACCATAGAACTGTGTGGCTTCCTGGGAAGTCACCCAACGAGGCAAACCTGATAGATTTTACCAA TGAGGCTGTGGAGCAAGTGGAAGAGGAGGAGTCAGGGGAGGCCCAGCTCTCATTTCTCACAGACAGTCAG ACAGTGACCAGCAGTGTGCTGGAGAAGGAGACTGAGCGGTTCCGTGAACTCTTGCTGTACGGTCGGAAGA AGGATGCTCTGGAGTCTGCCATGAAAAATGGCTTATGGGGTCACGCCCTGCTGCTTGCAAGTAAGATGGA CAGCCGGACACATGCCCGTGTCATGACCAGGTTTGCCAACAGTCTTCCGATCAATGACCCCCTGCAGACT GTCTACCAGCTCATGTCTGGACGGATGCCTGCTGCATCCACGTGTTGTGGAGATGAGAAATGGGGAGATT GGAGGCCACATCTTGCAATGGTTTTGTCCAACCTGAACAACAACATGGATGTGGAATCTCGGACCATGGC TACCATGGGTGATACCCTAGCATCAAAAGGCCTGCTGGATGCCGCACATTTTTGCTACCTCATGGCCCAG GTTGGATTTGGGGTTTATACAAAGAAAACTACCAAACTTGTTTTGATAGGCTCAAATCACAGTTTGCCAT TTTTAAAGTTTGCCACTAATGAAGCCATTCAAAGGACGGAGGCTTATGAGTACGCTCAGTCCCTTGGGGC GCATACCTGTTCCTTACCTAACTTCCAGGTGTTTAAATTCATCTACTTGTGCCGCCTGGCTGAAATGGGA CTCGCTACACAGGCCTTCCACTACTGTGAAGTAATAGCCAAGAGTGTCCTGACACAGCCTACTGCATACT CCCCAGTGCTGATTAGCCAGTTGATTCAGATGGCTTCCCAGTTACGCCTCTTCGATCCTCAACTGAAGGA GAAGCCAGAGGAGGAGTCCTTTGTGGAGCCTGCCTGGTTGGTGCAGCTGCAGCACGTGGAGAGGCAGATC CAGGAGGGCACTGTGCTGTGGAACCAGGATGGAACTGAACCCCAGCAGTGTCACATCACATCGGGCTCTG AGGTGGAGCAGCCAGATGGCCCTGGACTCAACCAGCAGGCAGGGCCAAGGGCCGACAACCCTCTCCTGAT GCCAAGCACTGAGCCTTTGGTGCACAGCGTGCAGCTGCTGCCCACAGCTCCTCAGACATTGCCTGATGGC CAGCCTGCTCACCTTGCCAGGGTGCCCATGTTCCCAGTACCAATGTCTCGTCCCCCCCTGGAGCTGAGTG CTGCCTATGGACCCCCGGGGTCTGTACTTGGCTTCCCAGAATCCTCCAGATCTGATCCTGCAGTGCTGCA TCCTGGGCAGGCCCTACCACCCGCTACACCAGGTCTCCAGGAAAGTGGGCCACCACTCCAGGAGGCCAGA AGCCCAGATCCAGAAATGGTGCCACAGGGCTCACCTGTCAGACACTCCCCTCCTGAGCCCCGTGAAGAGG AGTTTGGCAAGAACGTCGCTGACCTGGGCTCCTCTAGAACAGCACAGGACTCGGAAACCTCCCCAGTGTG GGATCTTGGCAGCTCCAGTCTGACACGTGCCCCATCTTTGACACCTGATTCTGAAGGGAAGAAACCTGCA CAAGCTGTCAAAAAGGAGCCCAAGGAGCCCAAGAAGACCGAGTCCTGGTTCTCTCGCTGGCTGCCTGGGA AGAAAAGGACAGAAGCTTATCTACCAGATGACAAGAACAAATCAATTGTTTGGGATGAAAAGAAAAACCA GTGGGTGAATCTGAATGAACCGGAGGAGGAGAAGAAGGCTCCACCCCCACCTCCAACATCGTTCCCCAGG GTTCCCCAGGCGGCTCCCACTGGGCCTGCAGGACCACCCATGGCCTCCGTGAATGTGTTTTCTAGAAAAG CAGGTGGGTCCAGAGCTCGCTATGTGGATGTTCTCAACCCAAGTGGAACACAGCGGAGTGAACCAGCTCT TGCTCCTGCGGATTTCTTTGCTCCTCTTGCCCCGCTGCCAATCCCTTCTAACTTATTTGTACCAAACCCA GATGCAGAAGAGTCACAACCTGCAGATGGGACTGGCTGTAGGGGACAGGCACCAGCTGGGACTCAATCTA AGGCAGAGCCCACCCTGGAAGCCAAGGTGGTAAGCTCTACAGTATCAGCCCCTGGGCCTGAACTCTCACC CTCCAAACCAGCAGATGGCTCCCAGGGAGGAGAGGCTCCTGGAGACCACTGTCCTGCAGGGGCCACTCCT GGTGGGTCTGTGCCCTTTTACAACCCTGCGCAGTTGGTGCAGGCGTCTGTCACCTCGGGGACTTCAAGGC CAGGAAGGATTGGCCAAAGAAAATATGCGGCACTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217765 representing NM_001276417 Red=Cloning site Green=Tags(s) MQPPPQAVPSGVAGPPPAGNPRSSFWANSPYRKPANNNAPVAPITRPLQPVTDPFAFNRQTLQNTPVGSS SKSSPPNLPGPALPVFSQWPGLPVPPTNAGDSSTGLHEPLPGTLSQPRADPSLFPPASTPSSLPGLEVNR SAEVDSSSGPEVQMLPHPPHYIPGVGPEQPHGGHLNDSGAGPDQSMNRHAPHDGAVAHAASPFFPQPQMP GQWGPVHGGPPPSYHHHSPCLEGPVQDMGPQAASLPHFPSPSSLHEGPGHESRVPQTFTPASLASGEGNE IVHQQSKNYPSSSFPPKHSFEQNSRVGNMWASAEFRQNPGVNKEHLLDPAHVNPFTQGNSPENQVHHPPV AATNHALQEAASGALSMFFQGEETENEENLSSEKAGLDDRLDSGSFSSTSRLGHPPLPGAGGVYQAAQEG DVQPYFSQFVGIRHDKQTTMTPANDAWGDIPGAGTHCASGPQCENVENLEFVQNQEVLPRETVNVDPFSP SDQFRYGPLPGPAASRPVTSGLTRGGGLNLEAPDMPLHPTRPDSVSSSYSNHSHRSPPGSARPQELVGTF IQQEVGKLEDDTSGSFFKQIDSSPVGGETDEMTRSQNYCSSLSQPSTPSPPKPTGVFQTSANSSFEPVKS HLVGVKPVEADRANMVVEVRGTQNCQKHGAAVLPPDATPGNLEQPPDNMETLCAPQACPLPLSTTGEAGQ LVSHTAGPPLDTVRPMPDKRSSARAQGPVKCESPATTLWAQNELPDFGGNVLLAPAAPALYVPVKPKPSE VVHHPEKGMSGQQSWKSGSVPPLQNQDPPGASENLENPPKVGEEEALPLQASSGYASLLSSPPTESLHNQ PVLIAQPDQSYNLAQPINFSVSLPNPNEKNQCWGDAVVGEKSMVSTRALGADHEEHAALSAIPASTVTGS SLPNSVTQNCAPRGSGSSEMIASQPASWLVQQLSPQIPQSQHPNAEKGPSEFVNSPANGSVMLAPPANSA VIPNSNKGYHSRNPEEAIGALDFTLSRTLENPVRMCSPSHSNSPASQQPLANHPRQSGPGLHNQDHFYQQ VTKDAQDHRSLERGQSELVPPPPQNSPQQVPQAACPEPSNPESPPTQGQSEGLVQPSASPAPVNTGQQPQ PPQSSSASVTSTNSNQAAVRSEQPWLHPPPTTFGPAPQDLASYYYYRPPYEAYQSPYPSPYPSDPGAASL YYQDIYGLYEPRYRPYDSSASAYAENHRYSEPERPSSRASHYSDQLTPRQGYPEGYYNSKSGWSSHSDYY ANYYSGQYDYGDPGRWDRYYGSRFRDPRTWDRRYWYDSDHDPYRKDNYAYSDRPEKCDDHWRYDPRFTGS FDDDTELHRDPYGEEVDRRSIHSEHSARSLRSTHSLPSRRSSLSSHSHQSQIYRSHHVTGGSFEAPHAPG SFHGDYAYGTYASNFSGAHGFPEYSYPADTSWPAAEQVPSRPTSPEKFTVPHVCARFGPGGQLLKVIPNL PSEGQPALVEIHSLETLLQHTPEQEEMRSFPGPLGKDDTHKVDVINFAQNKATKCLQNESLIDKESASLL WNFIILLCRQNGTVVGTDIAELLLRDHRTVWLPGKSPNEANLIDFTNEAVEQVEEEESGEAQLSFLTDSQ TVTSSVLEKETERFRELLLYGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRFANSLPINDPLQT VYQLMSGRMPAASTCCGDEKWGDWRPHLAMVLSNLNNNMDVESRTMATMGDTLASKGLLDAAHFCYLMAQ VGFGVYTKKTTKLVLIGSNHSLPFLKFATNEAIQRTEAYEYAQSLGAHTCSLPNFQVFKFIYLCRLAEMG LATQAFHYCEVIAKSVLTQPTAYSPVLISQLIQMASQLRLFDPQLKEKPEEESFVEPAWLVQLQHVERQI QEGTVLWNQDGTEPQQCHITSGSEVEQPDGPGLNQQAGPRADNPLLMPSTEPLVHSVQLLPTAPQTLPDG QPAHLARVPMFPVPMSRPPLELSAAYGPPGSVLGFPESSRSDPAVLHPGQALPPATPGLQESGPPLQEAR SPDPEMVPQGSPVRHSPPEPREEEFGKNVADLGSSRTAQDSETSPVWDLGSSSLTRAPSLTPDSEGKKPA QAVKKEPKEPKKTESWFSRWLPGKKRTEAYLPDDKNKSIVWDEKKNQWVNLNEPEEEKKAPPPPPTSFPR VPQAAPTGPAGPPMASVNVFSRKAGGSRARYVDVLNPSGTQRSEPALAPADFFAPLAPLPIPSNLFVPNP DAEESQPADGTGCRGQAPAGTQSKAEPTLEAKVVSSTVSAPGPELSPSKPADGSQGGEAPGDHCPAGATP GGSVPFYNPAQLVQASVTSGTSRPGRIGQRKYAALN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276417
ORF Size	7038 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276417.1, NP_001263346.1
RefSeq Size	8889 bp
RefSeq ORF	7041 bp
Locus ID	100360302
MW	253.3 kDa

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