Ubr2 (NM_001178071) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217688 representing NM_001178071 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGAGATGGAGCCCGAGGTGCAGGCCATCGACCGCAGTTTGCTGGAATGTTCTGCCGAGGAGA TCGCAGGGAAATGGCTGCAAGCAACCGACCTCACCAGAGAAGTATACCAGCATTTAGCCCATTGTGTGCC CAAAATCTACTGCCAGGGCCCCAACCCTTTCCCTCAGAAGGAAGACACACTGGCACAGCACATCTTGCTG GGACCAATGGAGTGGTATATCTGCGGCGAAGACCCTGCGCTGGGGTTTCCAAAGCTGGAGCAGGCGAACA AGCCTTCTCACCTCTGTGGCCGAGTGTTTAAAGTAGGGGAACCTACCTACTCCTGCAGAGACTGTGCAGT CGACCCCACCTGCGTTTTATGCATGCAGTGCTTCTTGGGAAGTATCCACAGAGACCATCGATACAGGATG ACCACATCAGGAGGTGGCGGCTTCTGTGACTGTGGTGACACTGAAGCGTGGAAAGAGGGTCCTTACTGCC AAAGACACGAGCTTAGCAGTTCAGAAGTTGTGGAGGAGGAGGATCCTCTTGTGCATCTGTCAGAAGACGT GATAGCAAGAACGTACAACATTTTTGCTATTATGTTTCGATACGCAGTAGATATGCTGACCTGGGAAAAA GAAAGTGAATTGCCGGAAGACTTAGAGGTGGGAGAGAAGACTGACACCTACTACTGCATGCTGTTTAACG ATGAGGTTCACACCTATGAGCAAGTCATTTATACTCTTCAGAAAGCTGTGAACTGTACTCAGAAAGAAGC CATTGGCTTTGCAACTACAGTCGATCGAGATGGGCGTAGGTCTGTCCGGTATGGAGATTTCCAGTACTGT GATCAAGCAAAGACAGTCATTGTGAGGAACACCAGCAGGCAGACCAAGCCGCTCAAAGTTCAGGTTATGC ACTCCTCCGTGGCCGCTCATCAGAATTTTGGTTTGAAAGCTCTGTCTTGGCTGGGAAGTGTTATTGGATA CTCAGATGGCCTTCGCCGGATTTTGTGTCAAGTTGGATTACAAGAAGGTCCAGATGGTGAAAACTCTTCT CTGGTCGACAGACTGATGCTTAATGATTCCAAATTATGGAAAGGCGCTCGGAGTGTGTACCACCAGCTGT TCATGAGCAGCCTGCTCATGGATCTCAAGTATAAGAAGCTGTTTGCTGTTCGCTTTGCAAAAAACTATGA GCGTTTGCAGAGAGATTATGTGACAGATGACCACGACAGAGAGTTTTCAGTCGCAGACCTCTCGGTTCAG ATATTCACAGTCCCTTCGCTTGCTCGAATGCTCATCACAGAAGAGAACCTGATGACTGTTATCATTAAGG CCTTCATGGATCATTTGAAACACAGAGATGCCCAAGGCAGATTCCAATTTGAACGTTACACTGCCCTCCA AGCCTTCAAGTTCAGGAGAGTTCAGAGCCTTATCTTAGATCTCAAGTATGTGTTGATCAGCAAACCAACG GAGTGGTCAGATGAGCTGAGGCAGAAGTTCTTGCAAGGCTTCGATGCCTTCTTGGAATTACTGAAGTGCA TGCAGGGAATGGATCCAATCACACGGCAGGTAGGACAGCACATTGAAATGGAGCCAGAGTGGGAGGCGGC CTTCACGCTGCAAATGAAACTGACGCACGTGATTTCCATGGTGCAGGATTGGTGTGCTTTGGACGAAAAA GTGTTAATTGAAGCTTACAAGAAATGCCTGGCTGTGCTGATGCAGTGTCATGGGGGATTTACTGATGGGG AACAGCCAATCACACTCAGTATTTGTGGACACTCGGTGGAAACGATCAGATACTGTGTTTCCCAAGAAAA AGTTAGCATTCACCTCCCAATTTCTCGCTTGCTTGCAGGTTTGCATGTGTTATTAAGCAAAAGTGAAGTG GCATATAAATTCCCCGAGCTCCTACCTCTAAGTGAACTGAGCCCACCCATGTTGATAGAGCATCCTCTTA GATGTCTTGTCTTATGTGCCCAAGTGCATGCTGGGATGTGGAGAAGAAATGGCTTCTCTCTAGTAAACCA GATCTATTACTACCATAATGTGAAATGCAGGCGGGAGATGTTCGACAAGGACATAGTGATGCTTCAGACA GGTGTCTCCATGATGGATCCAAACCACTTCCTGATGATCATGCTCAGCCGCTTTGAGCTCTATCAGATCT TCAGCACGCCTGACTACGGCAAGAGATTCAGTTCCGAGGTTACCCATAAGGACGTTGTTCAGCAGAACAA CACTCTGATAGAAGAGATGCTCTACCTCATCATCATGCTCGTCGGAGAAAGATTCAGCCCTGGGGTTGGA CAGGTGGGCGCCACAGATGAAATCAAGCGGGAGATTATCCATCAGCTGAGCATCAAGCCCATGGCGCACA GCGAGCTGGTGAAGTCTCTGCCCGAAGACGAGAACAAGGAGACCGGCATGGAGAGTGTCATCGAGACCGT AGCACATTTCAAGAAACCTGGTCTGACAGGACGAGGCATGTACGAGCTGAAGCCAGAGTGTGCCAAAGAG TTCAACCTGTATTTTTATCATTTCTCCAGGGCAGAGCAGTCCAAGGCGGAGGAAGCTCAGCGGAAGTTGA AAAGAGAAAATAAAGAAGATACAGCACTTCCTCCTCCGGCTTTGCCACCGTTCTGCCCTCTGTTCGGGAG CCTGGTTAACATCTTGCAGTGTGATGTCATGCTGTCCATCATGGGAACAATACTGCAGTGGGCTGTAGAG CGCCATGGGTCTGCCTGGTCAGAGTCTATGCTGCAAAGGGTGCTGCATTTGATTGGGATGGCGCTCCAGG AAGAGAAGCACCACTTGGAGAATGCTGTGGAAGGGCACGTGCAGACGTTCACCTTCACGCAGAAGATTTC AAAACCTGGCGATGCACCGAATAACTCCCCAAGCATCCTCGCTATGCTAGAGACCTTGCAGAACGCTCCC TCCCTGGAGGTCCACAAGGACATGATTAGGTGGTTGCTAAAGATGTTTAATACAATTAAGAAGATAAGAG AGAGTTCATCCAGCAGCCCTGTGGCAGAGGCAGAAGGAGCCATAATGGAAGAGAGCTCAAGAGACAAAGA CAAAGCAGAGAGAAAAAGGAAAGCGGAGATCGCCAGACTGCGCCGAGAGAAGATCATGGCTCAGATGTCC GAGATGCAGAGGCACTTCATTGACGAGAACAAAGAGCTCTTCCAGCAGACCATAGAGCTGGACGCCTCTG CCTCTGCCGCTCTCGACAACAGCCCTCTGGTTCCAGACGCGGCTCTGACAGCATTGGGTCCGGCACAGAC ACAGGTCCCTGAGCCCAGACAGTTTGTCACCTGTATACTGTGTCAGGAGGAGCAGGAGGTCACTGTGGAA AGCAGAGCAATGGTCTTGGCAGCGTTTGTTCAGAGGTCAACTGTGCTGTCAAAAGACAGATCGAAAGTCA TTGAGGACCCAGAAAAATATGACCCACTATTCATGCACCCTGATCTGTCCTGTGGAACACACACTGGCAG CTGTGGGCACGCCATGCACGCCCATTGTTGGCAAAGGTATTTTGATTCCGTTCAAGCTAAGGAGCAGCGA AGGCAGCAGCGACTGCGCCTGCACACTAGCTACGATGTAGAGAACGGCGAGTTCCTCTGCCCGCTCTGTG AGTGCCTGAGCAACACCGTGATCCCCCTGCTGCTTCCTCCCAGGAGCATCCTCAGCAGGAGGTTAAATTT TTCAGACCAACCAGATCTGGCACAGTGGACCAGAACAATAGCCCAGCAGATAAAGGTGATCCAGATGCTA AGGAAAGGAGACAGTGCTGCTGACAAGCCCTCTTCAGAGGACACAGAAGACATGAATATAATTCCCATCC CAGAAGGCTTCAGGCCTGATTTTTATCCTCGGAACCCATATTCCGATAGCATAAAGGAGATGCTAACAAC GTTTGGAACAGCTACTTACAAGGTGGGACTAAAGGTTCATCCCAATGAAGATGACCCCCGTGTACCCATC ATGTGCTGGGGGACCTGTGCATACACTATCCAGAGCATAGAAAGAATTCTGAGTGAGGAGGAAAAGCCCG TGTTCGGACCTCTGCCCTGCAGACTGGACGACTGTCTCAGGTCCTTAACACGCTTTGCAGCAGCACATTG GACAGTGGCGTTACTTCCCGTGGTACAAGGACACTTCTGTAAACTCTTTGCATCCTTGGTGCCTAGTGAC GGCTCTGAAGACCTCCCGTGCATATTAGACATCGACATGTTTCATTTGCTGGTGGGCCTGGTGCTCTCCT TCCCGGCTCTGCAGTGTCAGGATTTCTCAGGAAGCAGCCTGGCCACGGGAGACCTGCACATCTTCCACCT GGTTACTCTGGCACACATCGTACAGATCTTACTTACCTCATGTACAGAAGAGAATGGCATGGATCAAGAG AATCCCACTGGGGAAGAAGAACTCGCCATTCTCTCTTTGCACAAAACACTTCACCGGTGTACTGGAAGTG CCTTGGACGAAGCCCCATCCGGCTGGCACCTGTGGAGGAGCGTGCGGGCTGGCATCCTGCCTTTCCTCAA GTGCTCTGCTCTGTTCTTCCACTATTTAAATGGAGTCCCGGCCCCACCAGACCTGCAAGTTTCTGGAGCA AGCCATTTTGAACACTTATGTAACTACCTTTCCCTACCAACCAACCTCATCCGCCTTTTTCAAGAAAACA GTGACATCATGAACTCTCTGATTGAAAGTTGGTGCCAGAACAGCGAAGTTAAAAGGTATCTAAACGGCGA GAGAGGAGCGATAAGCTACCCGAGAGGAGCCAACAAACTGATAGACCTTCCGGAGGACTACAGCAGCCTC ATCAACCAAGCTTCCAATTTCTCGTGCCCCAAATCAGGTGGTGACAAGAGCAGAGCCCCTACCCTGTGCC TCGTATGTGGGAGTCTCCTCTGCTCTCAGAGTTACTGCTGCCAAGCCGAGCTGGAGGGCGAGGATGTCGG GGCCTGCACAGCACACACCTACTCCTGCGGCTCTGGGGCCGGCATCTTCCTGAGAGTACGGGAATGTCAG GTGCTATTTTTAGCTGGCAAAACCAAAGGCTGTTTTTATTCTCCTCCTTACCTTGATGACTACGGGGAGA CTGACCAGGGACTCAGACGAGGAAATCCTTTACATTTATGCCAAGAGCGGTTTCGAAAGATCCAGAAGCT CTGGCAGCAGCACAGCATCACCGAGGAGATCGGACACGCGCAGGAGGCCAACCAGACCCTGGTGGGCATC GACTGGCAGCATTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217688 representing NM_001178071 Red=Cloning site Green=Tags(s) MASEMEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHCVPKIYCQGPNPFPQKEDTLAQHILL GPMEWYICGEDPALGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMQCFLGSIHRDHRYRM TTSGGGGFCDCGDTEAWKEGPYCQRHELSSSEVVEEEDPLVHLSEDVIARTYNIFAIMFRYAVDMLTWEK ESELPEDLEVGEKTDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYC DQAKTVIVRNTSRQTKPLKVQVMHSSVAAHQNFGLKALSWLGSVIGYSDGLRRILCQVGLQEGPDGENSS LVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYERLQRDYVTDDHDREFSVADLSVQ IFTVPSLARMLITEENLMTVIIKAFMDHLKHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPT EWSDELRQKFLQGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMVQDWCALDEK VLIEAYKKCLAVLMQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPISRLLAGLHVLLSKSEV AYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQT GVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFSPGVG QVGATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIETVAHFKKPGLTGRGMYELKPECAKE FNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFGSLVNILQCDVMLSIMGTILQWAVE RHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPNNSPSILAMLETLQNAP SLEVHKDMIRWLLKMFNTIKKIRESSSSSPVAEAEGAIMEESSRDKDKAERKRKAEIARLRREKIMAQMS EMQRHFIDENKELFQQTIELDASASAALDNSPLVPDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVE SRAMVLAAFVQRSTVLSKDRSKVIEDPEKYDPLFMHPDLSCGTHTGSCGHAMHAHCWQRYFDSVQAKEQR RQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRTIAQQIKVIQML RKGDSAADKPSSEDTEDMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTATYKVGLKVHPNEDDPRVPI MCWGTCAYTIQSIERILSEEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSD GSEDLPCILDIDMFHLLVGLVLSFPALQCQDFSGSSLATGDLHIFHLVTLAHIVQILLTSCTEENGMDQE NPTGEEELAILSLHKTLHRCTGSALDEAPSGWHLWRSVRAGILPFLKCSALFFHYLNGVPAPPDLQVSGA SHFEHLCNYLSLPTNLIRLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSL INQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQ VLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGI DWQHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001178071
ORF Size	5265 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001178071.1, NP_001171542.1
RefSeq Size	5268 bp
RefSeq ORF	5268 bp
Locus ID	363188
MW	199 kDa

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