Stag2 (NM_001173507) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1562042
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217554 representing NM_001173507 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAGCAGCTCCAGAAATACCAACTGATTTTAATCTACTGCAGGAGTCAGAAACACATTTTTCTTCTG ACACAGATTTTGAAGACATTGAAGGAAAAAATCAGAAGCAAGGCAAAGGCAAAACTTGTAAAAAAGGCAA GAAGGGTCCAGCAGAGAAGGGTAAAAGTGGAAATGGAGGAGGAAAACCTCCTTCTGGTCCAAACCGAATG AATGGTCATCATCAGCAGAATGGAGTTGAAAACATGATGTTGTTTGAAGTTGTAAAAATGGGCAAGAGTG CTATGCAGTCGGTGGTAGATGATTGGATAGAATCATACAAGCATGACCGAGATATAGCACTTCTTGACCT TATCAACTTTTTTATTCAGTGTTCAGGCTGCAAAGGAGTCGTCACAGCAGAGATGTTTAGACATATGCAG AACTCTGAGATAATTCGAAAAATGACAGAAGAATTCGACGAGGATAGTGGGGATTACCCACTAACCATGG CTGGTCCTCAGTGGAAGAAGTTCAAGTCCAGCTTCTGTGAGTTCATTGGTGTATTAGTACGGCAATGTCA ATATAGTATCATATATGATGAGTACATGATGGACACTGTCATTTCACTTCTTACTGGATTGTCTGACTCC CAAGTCAGAGCATTTCGACATACAAGCACCCTGGCAGCTATGAAGTTGATGACTGCTTTGGTAAATGTGG CACTAAATCTTAGCATTAATATGGATAACACACAAAGACAGTATGAGGCAGAACGGAATAAAATGATTGG AAAACGAGCCAATGAAAGGCTAGAACTCCTGTTACAAAAGCGTAAAGAGCTTCAGGAAAATCAAGATGAA ATAGAAAATATGATGAATGCAATATTTAAAGGAGTGTTTGTACATAGATACAGGGATGCAATAGCTGAAA TCAGAGCTATATGCATTGAAGAGATTGGCATTTGGATGAAGATGTATAGTGATGCCTTCCTTAATGACAG CTATTTAAAATATGTTGGTTGGACTATGCATGATAAGCAAGGTGAGGTAAGACTCAAGTGTCTTACTGCT TTACAAGGGCTTTACTATAACAAAGAGCTTAACTCCAAACTGGAACTGTTTACCAGTCGGTTCAAGGATA GAATTGTGTCTATGACCCTTGACAAAGAATATGATGTTGCAGTACAAGCAATAAAGTTACTCACTCTTGT TTTACAGAGTAGTGAAGAAGTTCTAACTGCAGAAGACTGTGAAAATGTCTATCATCTGGTTTATTCAGCT CACCGGCCAGTAGCAGTTGCAGCTGGAGAATTTCTCTACAAAAAGCTCTTCAGCCGTAGAGATCCAGAAG AGGATGGAATAATGAAAAGAAGGGGAAGACAAGGTCCAAATGCCAATCTTGTCAAGACTTTGGTGTTTTT CTTCTTGGAAAGTGAGTTACATGAACATGCAGCATACCTTGTGGATAGCATGTGGGATTGTGCTACTGAG CTACTGAAAGACTGGGAATGTATGAATAGCTTGTTATTAGAGGAGCCTCTCAGTGGAGAGGAAGCTCTAA CAGACAGACAAGAGAGTGCTTTGATTGAAATAATGCTTTGTACTATTAGACAAGCAGCTGAATGTCATCC GCCTGTGGGAAGAGGGACAGGAAAAAGGGTGCTAACAGCAAAGGAGAAGAAGACACAGTTGGATGACAGA ACAAGAATCACTGAGCTGTTTGCTGTGGCCCTTCCACAATTATTAGCAAAATACTCTGTAGATGCAGAAA AGGTGACCAATTTGTTGCAGTTACCTCAATACTTTGACTTGGAGATCTACACCACTGGACGATTGGAAAA GCACTTGGATGCCTTATTGCGTCAGATCCGAAATATTGTAGAGAAGCACACAGACACAGATGTTTTGGAA GCATGTTCTAAAACTTACCATGCATTATGTAATGAAGAGTTCACGATCTTCAACAGAGTAGATATTTCAA GAAGTCAGCTGATAGATGAACTGGCAGATAAATTTAACCGGCTTCTGGAAGATTTTCTGCAAGAGGGAGA AGAACCTGACGAAGATGATGCATATCAGGTATTGTCAACATTGAAGAGGATCACTGCTTTTCATAATGCC CATGATCTTTCAAAGTGGGATTTGTTTGCTTGTAATTACAAACTCTTGAAAACCGGAATTGAAAATGGAG ATATGCCTGAGCAGATCGTTATTCACGCACTGCAGTGTGCTCACTATGTAATCCTTTGGCAGCTTGCTAA GATCACTGAAAGCACTTCTACAAAGGAGGACTTGCTGCGTTTAAAGAAACAGATGAGGGTATTTTGCCAG ATATGTCAACATTACTTAACCAACGTGAATACTACTGTTAAGGAACAGGCCTTCACTATTCTGTGTGATA TTTTGATGATCTTCAGTCACCAGATTATGTCTGGAGGGCGTGACATGTTAGAGCCATTAGTTTATACTCC TGATTCTTCATTGCAGTCTGAGTTGCTCAGCTTTATTTTGGATCATGTCTTCATTGAACAGGATGATGAT AGTAATAGTGCAGATGGTCAGCAGGAGGATGAAGCCAGTAAAATTGAAGCTTTGCATAAGAGGAGAAATT TACTTGCAGCATTTTGTAAGCTAATTGTATATACAGTGGTGGAGATGAACACAGCTGCGGATATCTTCAA GCAGTATATGAAGTATTATAACGATTATGGCGATATCATCAAAGAAACAATGAGTAAAACAAGGCAGATT GACAAAATTCAGTGTGCCAAGACTCTTATTCTCAGTTTGCAGCAGCTTTTTAATGAGATGATACAAGAAA ATGGCTATAACTTTGATAGATCATCCTCAACATTTAGCGGCATAAAAGAACTTGCTCGACGTTTTGCTTT AACTTTTGGACTTGATCAGTTGAAAACCAGAGAAGCCATTGCCATGCTGCACAAAGATGGCATAGAATTT GCTTTTAAGGAGCCTAATCCACAAGGGGAGAGCCATCCACCTTTAAATTTGGCATTTCTTGATATTCTCA GTGAATTTTCTTCTAAACTGCTCCGACAAGATAAAAGAACAGTGTATGTTTATTTGGAAAAGTTCATGAC CTTTCAGATGTCACTCCGAAGAGAAGATGTATGGCTTCCTCTGATGTCTTACCGAAATTCTTTGCTAGCT GGTGGTGATGATGACACCATGTCAGTCATTAGTGGAATGAGCAGTCGGGGATCAACTGTACGGAGTAAAA AATCAAAACCATCTACAGGGAAACGGAAAGTGCTTGAAGGCATGCAACTAGCACTCCCTGAAGAAAGCAG TAGCAGTGACAGTATGTGGTTGAGCAGAGAGCAGACACTGCACACCCCTGTTATGATGCAGACACCGCAG CTCACCTCCACTATTATGAGAGAACCGAAAAGGTTACGGCCTGAAGATAGCTTCATGAGTGTCTATCCGA TGCAGACAGAACACCATCAAACTCCTCTGGATTACAATCAGCGTGGTACAAGCATAATGGAGGATGACGA AGAGCCGATTGTTGAAGATGTTATGATGTCCTCAGAAGGAAGGATTGAGGATCTTAATGAGGGAATGGAC TTTGACACCATGGATATAGACCTGCCACCATCAAAGAATAGAAGAGAGAGAACAGAACTGAAGCCTGATT TCTTTGATCCTGCTTCAATTATGGATGAATCAGTTCTTGGCGTGTCAATGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217554 representing NM_001173507 Red=Cloning site Green=Tags(s) MIAAPEIPTDFNLLQESETHFSSDTDFEDIEGKNQKQGKGKTCKKGKKGPAEKGKSGNGGGKPPSGPNRM NGHHQQNGVENMMLFEVVKMGKSAMQSVVDDWIESYKHDRDIALLDLINFFIQCSGCKGVVTAEMFRHMQ NSEIIRKMTEEFDEDSGDYPLTMAGPQWKKFKSSFCEFIGVLVRQCQYSIIYDEYMMDTVISLLTGLSDS QVRAFRHTSTLAAMKLMTALVNVALNLSINMDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDE IENMMNAIFKGVFVHRYRDAIAEIRAICIEEIGIWMKMYSDAFLNDSYLKYVGWTMHDKQGEVRLKCLTA LQGLYYNKELNSKLELFTSRFKDRIVSMTLDKEYDVAVQAIKLLTLVLQSSEEVLTAEDCENVYHLVYSA HRPVAVAAGEFLYKKLFSRRDPEEDGIMKRRGRQGPNANLVKTLVFFFLESELHEHAAYLVDSMWDCATE LLKDWECMNSLLLEEPLSGEEALTDRQESALIEIMLCTIRQAAECHPPVGRGTGKRVLTAKEKKTQLDDR TRITELFAVALPQLLAKYSVDAEKVTNLLQLPQYFDLEIYTTGRLEKHLDALLRQIRNIVEKHTDTDVLE ACSKTYHALCNEEFTIFNRVDISRSQLIDELADKFNRLLEDFLQEGEEPDEDDAYQVLSTLKRITAFHNA HDLSKWDLFACNYKLLKTGIENGDMPEQIVIHALQCAHYVILWQLAKITESTSTKEDLLRLKKQMRVFCQ ICQHYLTNVNTTVKEQAFTILCDILMIFSHQIMSGGRDMLEPLVYTPDSSLQSELLSFILDHVFIEQDDD SNSADGQQEDEASKIEALHKRRNLLAAFCKLIVYTVVEMNTAADIFKQYMKYYNDYGDIIKETMSKTRQI DKIQCAKTLILSLQQLFNEMIQENGYNFDRSSSTFSGIKELARRFALTFGLDQLKTREAIAMLHKDGIEF AFKEPNPQGESHPPLNLAFLDILSEFSSKLLRQDKRTVYVYLEKFMTFQMSLRREDVWLPLMSYRNSLLA GGDDDTMSVISGMSSRGSTVRSKKSKPSTGKRKVLEGMQLALPEESSSSDSMWLSREQTLHTPVMMQTPQ LTSTIMREPKRLRPEDSFMSVYPMQTEHHQTPLDYNQRGTSIMEDDEEPIVEDVMMSSEGRIEDLNEGMD FDTMDIDLPPSKNRRERTELKPDFFDPASIMDESVLGVSMF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001173507
ORF Size	3693 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001173507.1, NP_001166978.1
RefSeq Size	6122 bp
RefSeq ORF	3696 bp
Locus ID	313304
MW	141.3 kDa

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