Cnnm4 (NM_001271050) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217195 representing NM_001271050 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGGGGGGCGGGGGCGGGCGGCGGGACGGCTGGCCGGCCCGAGGGCGCCTCCTCCTGGCCGCGC TGCTGCTGCTGCTATGGACGCGGGCGGCCAGCGGCCAGAGCAGCCCCCAGCAGAGCGTGATCCTAGGCAT GAGGCTGGCGAGCTGCAACAAGTCGTGCGGGATGAACCCGGATGGCATCATCTTCGTGTCCGAGGGCAGC ACGGTGAACTTGAGGCTCTACGGCCACAGACTGGGCGAGATCTCTAGCAACCTGATCTCCTTCACCGAGG TGGACGACGCCGAGACGGTCCACAACTCCACCAACTGCCTGGAGCTCACCAAGGACTTAGTGGTCCAGCG GCTGGTCAACGTGAGCCGCGGGAACACGTCGGGGATGCTAGTGGTGATTACCAAGTTCCTGCGGAGGAGT GAGAACATGAAGCTGTACGCGCTGTGCACCCGGACCCGGGCCGACGGGCCCTGGCTCAAATGGACGGACA AGGACTCCCTGCTCTTCATGGTGGAGGAGCATGGGAGGTTTCTGCCGCTCTGGCTGCACATCCTTCTTGT TTTGGTGCTGTTGGTGCTGTCGGGCATCTTTTCTGGCCTCAACTTGGGGCTTATGGCCCTGGACCCGATG GAGTTGCGCATCGTGCAGAACTGTGGAACTGAGAAGGAGAGAAGGTACGCTCGCAAGATAGAGCCCATCA GGCGCAAGGGCAACTACCTGCTCTGCTCGCTGCTCCTGGGGAATGTGCTAGTCAACACCTCCCTCACCAT CCTTCTAGACAACCTCATCGGTTCTGGCATCATGGCAGTGGCCTCCTCCACCATTGGCATTGTCATCTTT GGGGAGATCTTACCTCAAGCCCTGTGCTCCAGGCATGGGCTGGCTGTGGGTGCCAACACTATTGTTCTCA CCAAAATCTTTATGTTACTCACTTTCCCCCTGAGTTTCCCCATTAGCAAACTCCTGGACTTCGTCTTGGG TCAGGAGATTCGCACTGTTTACAATCGGGAGAAACTGATGGAGATGTTGAAGGTGACAGAGCCCTATAAT GACCTGGTGAAGGAGGAGTTAAACATGATCCAGGGTGCTCTGGAACTAAGGACCAAAACTGTGGAGGATA TCATGACCCAGCTACACGACTGCTTCATGATCCGCAGTGATGCCATTCTGGACTTCAACACCATGTCGGA GATTATGGAGAGTGGCTATACTCGCATTCCTGTGTTTGAAGATGAGCAGTCCAATATCGTAGATATCCTT TACGTCAAAGACCTGGCTTTTGTTGACCCAGATGACTGCACCCCGCTCAAGACGATCACTCGCTTCTACA ACCACCCGGTCCATTTTGTTTTTCATGACACCAAGCTGGATGCCATGCTGGAGGAATTCAAGAAGGGCAA GTCCCACCTGGCCATCGTGCAGAAGGTGAACAATGAGGGCGAGGGCGACCCCTTCTACGAGGTGCTGGGC CTGGTCACCCTGGAGGACGTCATCGAGGAGATCATCAAGTCTGAGATCCTGGATGAGTCGGACACGTACA CTGACAATCGAACCCGGAAACGGGTGTCCATGAAGAACAAGCGTGACTTCTCTGCCTTCAAGGATGCTGA CAATGAACTCAAAGTGAAAATCTCACCTCAGCTTCTTCTGGCCGCTCACCGCTTCCTGGCCACAGAGGTG CCGCAGTTCAGTCCCTCCCTGATGTCAGAGAAGATCCTGCTCCGGCTGCTCAAGTACCCTGACGTCATTC AGGAGCTCAGGTTCGACGAGCACAACAAGCACTGCACCCGCCACTACCTGTACACCCGGAACAAGCCAGC CGACTGCTTCATTCTCATCCTGCAGGGGAAGGTGGAGGTGGAAGCAGGCAAGGAGAACATGAAGTTTGAG ACCGGTGCCTTCTCGTACTATGGGACCATGGCTCTGTCCCTGGCGCCCCCAGACCGGTCCCCAGCCCACC CAACTCCACTCAGCCGTTCAGCCTCCCTCAGTTACCCGGACCGCAACACAGACATGACACCCTCCTCCCT GGCAGGCAGCAACCAGTTTGGCAGCTGCATTCTGGGCCAGTATGTCTCTGACTTCAGTGTGCGGGCGCTC ACAGACCTGCAGTACATTAAGGTCACAAGACAGCAGTACCAGAACGGGCTGCTGGCCTCCCGCATGGACA ACAGCCCCCAGCTCACCTTGGATGGCTGTGCCACCTGCACAGAGAACTTATCCGAGAGGCCTGAGCTGCC CGTGGTGGACGAGACGACGACTCTTCTCAATGAACGCAACTTGTTGCTGCACAGAGCCTCCCAAGAGGGC ACCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217195 representing NM_001271050 Red=Cloning site Green=Tags(s) MAPGGGGGRRDGWPARGRLLLAALLLLLWTRAASGQSSPQQSVILGMRLASCNKSCGMNPDGIIFVSEGS TVNLRLYGHRLGEISSNLISFTEVDDAETVHNSTNCLELTKDLVVQRLVNVSRGNTSGMLVVITKFLRRS ENMKLYALCTRTRADGPWLKWTDKDSLLFMVEEHGRFLPLWLHILLVLVLLVLSGIFSGLNLGLMALDPM ELRIVQNCGTEKERRYARKIEPIRRKGNYLLCSLLLGNVLVNTSLTILLDNLIGSGIMAVASSTIGIVIF GEILPQALCSRHGLAVGANTIVLTKIFMLLTFPLSFPISKLLDFVLGQEIRTVYNREKLMEMLKVTEPYN DLVKEELNMIQGALELRTKTVEDIMTQLHDCFMIRSDAILDFNTMSEIMESGYTRIPVFEDEQSNIVDIL YVKDLAFVDPDDCTPLKTITRFYNHPVHFVFHDTKLDAMLEEFKKGKSHLAIVQKVNNEGEGDPFYEVLG LVTLEDVIEEIIKSEILDESDTYTDNRTRKRVSMKNKRDFSAFKDADNELKVKISPQLLLAAHRFLATEV PQFSPSLMSEKILLRLLKYPDVIQELRFDEHNKHCTRHYLYTRNKPADCFILILQGKVEVEAGKENMKFE TGAFSYYGTMALSLAPPDRSPAHPTPLSRSASLSYPDRNTDMTPSSLAGSNQFGSCILGQYVSDFSVRAL TDLQYIKVTRQQYQNGLLASRMDNSPQLTLDGCATCTENLSERPELPVVDETTTLLNERNLLLHRASQEG TI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271050
ORF Size	2316 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271050.1, NP_001257979.1
RefSeq Size	4599 bp
RefSeq ORF	2319 bp
Locus ID	363216
UniProt ID	P0C588
MW	86.7 kDa
Gene Summary	Probable metal transporter. The interaction with the metal ion chaperone COX11 suggests that it may play a role in sensory neuron functions. May play a role in biomineralization and retinal function.[UniProtKB/Swiss-Prot Function]

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