Aldh7a1 (NM_001271105) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	antiquitin
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216874 representing NM_001271105 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCGTCTGGCTCGCCCGCTCTGCGTGCAGACTGTGAAGGCCAGCAAACTTTCCCGCCTTTGGAGTA GGCCTGCCGCCCTTATGTCTACTCTGCTGATCCATCATCCCCAGTATGCCTGGCTGCAAGACCTGGGTCT CCGCGAAGATAACGAGGGCGTGTTTAATGGAAGCTGGGGCGGCCGGGGAGAGGTAATTACGACCTATTGT CCTGCTAACAATGAGCCAATAGCAAGAGTCCGACAGGCCAGCATGAAAGACTATGAAGAGACTATAGGGA AAGCCAAGAAAGCTTGGAACATCTGGGCAGATATTCCTGCCCCAAAGCGAGGAGAAATAGTCAGAAAGAT TGGCGATGCCTTGCGGGAGAAGATCCAATTACTGGGAAGACTGGTGTCTTTGGAGATGGGGAAAATCTTA GTGGAAGGAATAGGGGAGGTTCAGGAGTACGTGGACGTCTGCGATTATGCTGCCGGCTTGTCGAGGATGA TCGGGGGACCCACCTTGCCCTCTGAAAGACCCGGCCATGCTCTCATGGAACAGTGGAATCCGTTAGGCCT GGTGGGAATCATCACTGCCTTCAACTTCCCTGTGGCTGTGTTTGGCTGGAACAATGCCATAGCTCTGATC ACAGGCAACGTCTGCCTTTGGAAAGGAGCACCAACAACTTCCCTCGTTAGTATAGCTGTCACAAAGATCA TAGCCAAGGTTTTGGAGGACAACCTGCTGCCTGGTGCCATTTGCTCCCTGACCTGTGGTGGAGCAGATAT GGGCACAGCAATGGCCAGAGATGAGCGTGTGAACCTGCTGTCGTTCACTGGGAGCACTCAGGTGGGGAAG CAGGTGGCCCTCATGGTGCAGGAGAGGTTTGGGAAAAGCTTGTTGGAGCTCGGAGGAAACAATGCCATCA TTGCTTTCGAGGACGCCGACCTCAGCTTGGTTCTTCCCTCCGCTCTGTTTGCCGCCGTGGGAACAGCCGG CCAGAGGTGTACCACTGTGAGGCGACTGTTTTTGCACGAAAGCATCCATGATGAAGTTGTAGACAGACTG AAAAATGCCTACTCACAGATCCGTGTCGGGAACCCCTGGGACCCCAATATCCTCTATGGACCGCTCCACA CCAAACAGGCGGTGAGCATGTTTGTGCAAGCCGTGGAAGAAGCAAAGAAAGAAGGAGGCACGGTGGTCTA TGGGGGCAAGGTCATGGACCACCCTGGCAATTATGTGGAACCCACCATTGTGACTGGTCTTGTCCATGAT GCGCCCATTGTTCACAAGGAGACTTTTGCCCCAATTCTCTATGTCTTCAAATTCAAGAATGAAGAAGAGG TCTTTGAATGGAACAATGAAGTAAAACAGGGACTTTCGAGTAGTATCTTTACCAAGGATCTGGGCAGAAT CTTCCGCTGGCTTGGACCTAAGGGTTCAGACTGTGGCATTGTGAACGTCAATATTCCTACCAGCGGGGCT GAGATCGGCGGTGCGTTTGGAGGCGAGAAGCACACGGGCGGTGGCAGGGAATCTGGCAGCGATGCCTGGA AGCAGTACATGCGGAGATCTACGTGTACCATCAACTACAGCACGGCCCTCCCTCTGGCTCAAGGTATCAA GTTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216874 representing NM_001271105 Red=Cloning site Green=Tags(s) MLRLARPLCVQTVKASKLSRLWSRPAALMSTLLIHHPQYAWLQDLGLREDNEGVFNGSWGGRGEVITTYC PANNEPIARVRQASMKDYEETIGKAKKAWNIWADIPAPKRGEIVRKIGDALREKIQLLGRLVSLEMGKIL VEGIGEVQEYVDVCDYAAGLSRMIGGPTLPSERPGHALMEQWNPLGLVGIITAFNFPVAVFGWNNAIALI TGNVCLWKGAPTTSLVSIAVTKIIAKVLEDNLLPGAICSLTCGGADMGTAMARDERVNLLSFTGSTQVGK QVALMVQERFGKSLLELGGNNAIIAFEDADLSLVLPSALFAAVGTAGQRCTTVRRLFLHESIHDEVVDRL KNAYSQIRVGNPWDPNILYGPLHTKQAVSMFVQAVEEAKKEGGTVVYGGKVMDHPGNYVEPTIVTGLVHD APIVHKETFAPILYVFKFKNEEEVFEWNNEVKQGLSSSIFTKDLGRIFRWLGPKGSDCGIVNVNIPTSGA EIGGAFGGEKHTGGGRESGSDAWKQYMRRSTCTINYSTALPLAQGIKFQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271105
ORF Size	1617 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271105.1, NP_001258034.1
RefSeq Size	1894 bp
RefSeq ORF	1620 bp
Locus ID	291450
UniProt ID	Q64057
MW	58.7 kDa
Gene Summary	Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism (By similarity).[UniProtKB/Swiss-Prot Function]

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