Panx1 (NM_001270548) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	px1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216461 representing NM_001270548 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATCGCCCACCTGGCCACGGAGTACGTGTTCTCGGACTTCTTGCTGAAGGAGCCCACCGAGCCCA AGTTCAAGGGGCTGCGACTGGAGCTGGCAGTGGACAAGATGGTCACGTGCATTGCCGTGGGTCTGCCTCT GCTGCTCATCTCGCTGGCCTTCGCTCAGGAGATCTCCATCGGTACCCAGATAAGCTGCTTCTCCCCAAGT TCCTTCTCCTGGCGCCAGGCCGCCTTCGTGGACTCTTACTGCTGGGCCGCTGTACAGCAGAAAAACTCCC TGCAGAGTGAGTCTGGAAACCTCCCGCTGTGGCTGCACAAGGTAATAATGAGTCTGTGGGAGATATCCGA AAGCCACTTCAAGTACCCGATCGTGGAGCAGTACTTGAAGACGAAGAAGAACTCCAGTCACCTAATCATG AAATACATTAGCTGCCGGCTGGTGACCTTTGCGGTGGTACTGCTGGCTTGCATCTACTTGAGCTACTACT TCAGCCTCTCCTCGCTCTCCGACGAGTTTCTGTGTAGCATCAAGTCGGGCGTCCTGAGGAACGACAGCAC CATCCCCGATAGCTTCCAGTGCAAGCTCATCGCGGTCGGCATCTTCCAGCTGCTCAGCCTCATTAACCTC CTAGTGTACGCCCTGCTGGTCCCCGTGGTCATCTACACGCTCTTCGTCCCGTTCCGGCAGAAGACGGACG TCCTCAAGGTATACGAGATCCTGCCCACCTTCGATGTTCTACATTTCAAGTCTGAAGGCTACAACGACTT GAGCCTCTACAACCTTTTTCTGGAAGAGAACATAAGTGAGCTCAAATCGTACAAGTGTCTTAAGGTGCTG GAGAACATTAAGAGCAATGGGCAGGGCATCGACCCCATGTTACTTCTGACAAACCTAGGCATGATTAAGA TGGACGTCATTGATGGAAAAGTCCCCATGTCCCTACAGACCAAGGGAGAGGACCAGGGCAGCCAGAGAAT GGACTTCAAAGATTTGGACCTGAGCAGCGAGACGGCAGCGAACAATGGGGAGAAGAACTCTCGCCAGCGA CTTCTGAATTCATCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216461 representing NM_001270548 Red=Cloning site Green=Tags(s) MAIAHLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISIGTQISCFSPS SFSWRQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKVIMSLWEISESHFKYPIVEQYLKTKKNSSHLIM KYISCRLVTFAVVLLACIYLSYYFSLSSLSDEFLCSIKSGVLRNDSTIPDSFQCKLIAVGIFQLLSLINL LVYALLVPVVIYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISELKSYKCLKVL ENIKSNGQGIDPMLLLTNLGMIKMDVIDGKVPMSLQTKGEDQGSQRMDFKDLDLSSETAANNGEKNSRQR LLNSSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270548
ORF Size	1068 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270548.1, NP_001257477.1
RefSeq Size	2246 bp
RefSeq ORF	1071 bp
Locus ID	315435
MW	40.2 kDa
Gene Summary	The protein encoded by this gene is a plasma membrane protein that is a structural component of gap junctions. The encoded protein acts as a homodimer or as a heterodimer with other isoforms or proteins. Two additional variants have been found, and the isoforms expressed from them are found in the cytoplasm. It is thought that these two isoforms could attenuate the actions of the membrane-bound protein. [provided by RefSeq, Jul 2012]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.