Hcfc1 (NM_001139507) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1563804
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214974 representing NM_001139507 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGCTGTGTCCCCCGCTAACTTGCCAGCGGTGCTTCTACAGCCCCGCTGGAAACGGGTGGTGG GTTGGTCGGGTCCCGTGCCGCGACCCCGCCACGGCCACCGTGCAGTAGCTATCAAGGAGCTTATAGTGGT GTTTGGCGGCGGCAATGAGGGGATAGTGGACGAACTACACGTGTACAACACTGCGACCAACCAGTGGTTC ATCCCAGCTGTGAGAGGGGATATCCCTCCAGGGTGTGCAGCCTATGGCTTTGTATGTGATGGTACTCGCC TGCTGGTGTTTGGTGGAATGGTAGAGTATGGAAAATACAGCAACGACCTCTATGAGCTCCAGGCAAGTCG CTGGGAATGGAAGAGACTGAAGGCAAAGACACCCAAAAACGGGCCTCCTCCGTGTCCTCGGCTTGGACAC AGTTTCTCCCTTGTGGGCAACAAATGTTACCTGTTTGGGGGTCTGGCCAATGATAGTGAGGACCCCAAGA ACAACATTCCGAGGTACCTGAATGACTTATATATTCTCGAACTACGGCCAGGCTCTGGAGTGGTAGCTTG GGACATTCCCATCACTTATGGTGTCCTTCCTCCACCCCGGGAGTCACATACTGCTGTGGTCTACACTGAA AAAGATAACAAGAAATCCAAGCTGGTGATCTATGGAGGGATGAGTGGCTGCAGGCTAGGGGACCTTTGGA CCCTGGACATTGAGACACTGACATGGAATAAGCCCAGCCTTAGTGGGGTGGCACCCCTTCCTCGCAGCCT CCACTCTGCAACCACCATAGGAAACAAAATGTATGTATTTGGTGGCTGGGTGCCCCTTGTCATGGACGAT GTCAAAGTGGCCACACACGAGAAGGAATGGAAGTGTACCAACACACTGGCTTGTCTCAACCTGGATACCA TGGCCTGGGAAACCATCCTGATGGATACACTGGAGGACAACATTCCTCGAGCTCGAGCAGGCCACTGTGC TGTTGCCATCAATACTCGCCTGTATATTTGGAGTGGCCGTGATGGCTATCGCAAGGCCTGGAACAATCAG GTCTGCTGCAAGGACCTGTGGTATCTGGAGACAGAAAAGCCACCACCCCCAGCCCGAGTTCAACTAGTAC GAGCCAACACCAACTCACTGGAGGTTAGCTGGGGTGCAGTGGCAACAGCCGACAGTTACCTTCTACAACT CCAGAAATATGACATTCCTGCCACAGCTGCTACGGCTACCTCCCCCACTCCCAATCCAGTCCCGTCTGTG CCTGCCAACCCTCCCAAGAGCCCTGCGCCAGCAGCAGCTGCACCTGCTGTACAACCACTGACCCAAGTAG GCATCACACTTGTGCCCCAGGCTGCCGCTGCACCCCCAAGCACAACCACCATCCAGGTCTTGCCGACAGT GCCAGGCAGCTCTATTTCTGTGCCCACTGCAGCCAGGACTCAAGGTGTCCCTGCTGTTCTCAAAGTGACT GGTCCTCAAGCTACAACAGGAACACCACTGGTCACCATGAGACCTGCAAGCCAGGCTGGAAAAGCTCCTG TCACTGTGACTTCCCTGCCTGCCAGTGTGAGAATGGTTGTACCCACTCAGAGTGCCCAGGGCACGGTGAT TGGCAGCAACCCACAGATGAGTGGGATGGCTGCATTGGCTGCTGCTGCTGCTGCCACACAGAAAATCCCT CCTTCCTCAGCACCCACAGTGCTAAGTGTCCCAGCAGGCACCACCATTGTCAAGACAGTGGCTGTGACAC CTGGTACAACCACTCTTCCAGCCACTGTGAAGGTGGCCTCCTCACCTGTTATGGTGAGCAACCCAGCTAC TCGAATGCTAAAGACTGCAGCTGCCCAAGTGGGGACATCTGTGTCCTCTGCTGCCAACACATCTACCCGC CCTATCATCACAGTACATAAATCAGGGACTGTAACAGTGGCCCAGCAAGCCCAGGTGGTGACCACGGTGG TAGGCGGAGTCACCAAGACCATCACTCTAGTGAAGAGCCCCATCTCTGTCCCGGGAGGCAGTGCTCTGAT TTCCAATCTTGGAAAAGTGATGTCAGTGGTCCAGACCAAACCAGTTCAGACTTCAGCAGTGACAGGCCAA GCATCTACAGGTCCTGTGACTCAGATCATCCAGACCAAAGGACCCCTGCCAGCAGGGACTATCCTGAAGT TGGTGACATCAGCAGATGGCAAGCCCACAACCATCATCACCACCACACAGGCTAGTGGGGCAGGGACCAA GCCCACTATCCTGGGCATCAGTAGTGTCTCTCCTAGCACCACCAAACCCGGCACAACTACCATCATTAAG ACCATTCCCATGTCAGCAATTATCACCCAGGCAGGCGCCACAGGTGTTACCAGCAGTCCTGGCATTAAGT CCCCCATCACAATTATCACCACCAAGGTGATGACTTCAGGAACAGGAGCACCTGCCAAAATCATCACTGC TGTCCCCAAGATTGCTACTGGCCATGGGCAACAAGGAGTGACCCAGGTGGTGCTAAAGGGGGCCCCTGGA CAGCCAGGCACCATCCTCCGCACTGTGCCCATGGGCGGCGTTCGCCTGGTCACCCCTGTCACCGTCTCCG CTGTCAAGCCAGCTGTCACCACATTGGTTGTGAAGGGCACCACAGGTGTTACAACACTAGGCACAGTGAC AGGCACTGTCTCCACCAGCCTTGCCGGAGCTGGGGCACATAGCACCAGTGCTTCTCTGGCTACACCTATC ACTACTTTGGGCACCATTGCTACTCTCTCAAGTCAGGTGATCAACCCTACTGCTATCACAGTGTCAGCTG CACAGACTACACTAACAGCTGCTGGTGGACTCACCACACCTACAATCACAATGCAGCCTGTCTCCCAGCC TACCCAGGTGACTCTGATCACAGCACCTAGCGGGGTTGAGGCCCAACCTGTACATGACCTTCCTGTGTCC ATTTTGGCCTCACCTACTACAGAGCAACCCACAGCAACAGTCACTATCGCTGACTCAGGCCAGGGTGATG TACAACCTGGCACTGTGACACTGGTGTGTTCCAACCCACCCTGTGAAACCCATGAAACAGGCACCACCAA CACAGCTACCACCACTGTTGTGGCTAACCTTGGGGGACATCCTCAGCCTACCCAGGTGCAGTTTGTTTGT GACAGACAGGAGGCAGCTGCTTCACTTGTGACCTCAGCTGTGGGACAACAGAATGGTAATGTGGTCCGTG TCTGTTCAAACCCCCCCTGTGAAACCCATGAGACAGGCACCACCAACACTGCCACAACAGCCACCTCAAA CATGGCTGGGCAGCATGGCTGCTCGAATCCTCCCTGCGAGACTCATGAGACAGGTACCACCAGCACTGCC ACTACAGCAATGTCCAGCATGGGCACTGGGCAGCAGCGAGACGCTCGTCGTGCCACTAACACCCCCACTG TAGTGCGGATCACTGTGGCTCCTGGGGCATTGGAAAGAGCCCAGGGTACTGTGAAGCCTCCGTGCCAAAC CCAGCAGACCAACATGACCAGCACCACCATGACTGTGCAGGCCACTGGAGCTCCATGCTCAGCTGGCCCA CTGCTCAGGCCAAGTGTGGCACTGGAGACTGGGAGCCATAGCCCTGCCTTCGTGCAACTAGCCCTTCCAA GTGTCAGAGTTGGGCTAAGTGGCCCCAGCAGCAAGGACGTGCCCACAGGGCGCCAACCAGAGACATATCA TACTTACACAACCAATACCCCAACCACAGCCCGTTCTATCATGGTTGCTGGGGAGCTTGGTACAGCTCGG GTGGTCCCCACATCTCAATATGATTGCCTCCAGGCAAGCTCTCCCAGTAGCACCATGACTATGACAGCCC TAGAGGCCCTGCTGTGCCCTTCAGCTACTGTGACCCAAGTCTGCTCCAACCCACCATGTGAGACCCATGA GACGGGTACCACCAACACCGCCACTACCTCCAATGCGGGCAGTGCTCAGCGAGTATGCTCTAACCCACCT TGTGAGACTCATGAGACGGGCACCACACACACAGCTACCACTGCCACATCAAATGGAGGCGCAGGCCAGC CTGAGGGTGGACAACAGCCTGCCAGTGGCCATCCCTGCGAGACACACCAGACCACTTCCACTGGCACCAC TATGTCAGTCAGTGTGGGTGCCCTGATTCCTGATGCCACTCCCTCTCATGGGACCCTGGAGTCTGGTTTA GAGGTGGTAGCAGTGCCCACTGTCACCTCCCAGGCTGGTGCCACATTGCTGGCCTCTTTCTCAACACAGA GGGTATGCTCCAATCCTCCTTGCGAGACCCACGAGACAGGTACAACGCACACAGCCACCACTGTCACCTC TAACATGAGCTCAAACCAAGACCCTCCACCAGCTGCCAGTGACCAAGGAGAAGTGGCGAGTACCCAAGGT GACAGCACAAATATCACCAGTGCCAGTGCTATCACTACAACTGTGTCTTCTACACTGCCAAGAGCAGTGA CCACTGTGACACAGTCTACACCAGTCCCAGGTCCTTCTGTGCCGCCCCCAGAGGAACTCCAGGTCTCACC AGGGCCTCGCCAGCAGCTGCCTCCAAGGCAACTCCTGCAGTCTGCCTCCACACCCCTGATGGGGGAGTCC GCCGAGGTCCTGTCAGCCTCCCAGACCCCTGAGCTCCAGGCCGCCGTGGATCTGAGCAGCACTGGGGACC CATCTTCAGTCCAGGAGCCTACCACCTCTGCTGTTGTGGCCACTGTGGTGGTCCAACCACCCCAACCCAC ACAGTCTGAAGTAGACCAGTTATCACTTCCCCAAGAGCTGATGGCTGAAGCCCAGGCGGGCACCACAACC CTTATGGTAACAGGGCTCACTCCAGAGGAGCTGGCAGTGACTGCTGCTGCTGAAGCAGCTGCTCAAGCTG CAGCCACTGAAGAAGCTCAAGCCTTGGCCATCCAGGCTGTGCTCCAGGCTGCACAGCAGGCTGTCATGGG CACTGGGGAGCCCATGGATACATCTGAAGCAGCAGCAGCAGTGACACAAGCAGAACTGGGTCATCTTTCA GCTGAAGGCCAAGAGGGTCAGGCTACCACCATACCCATTGTGCTGACACAGCAGGAGCTTGCAGCTCTTG TACAGCAGCAGCAGCAGCTCCAGGAGGCTCAAGCCCAAGCCCAGCAACAGCACCACCTTCCCACTGAGGC TCTGGCCCCAGCTGACAGTCTCAATGACCCATCCATCGAGAGCAACTGCCTCAATGAGTTAGCTAGTGCT GTCCCCAGCACTGTGGCCTTGCTACCCTCAACAGCTACTGAGAGTTTGGCTCCATCTAACACATTTGTGG CTCCTCAGCCTGTTGTTGTAGCCAGTCCAGCAAAGATGCAGGCTGCAGCAACCCTTACTGAAGTGGCAAA TGGCATTGAGTCCCTGGGTGTGAAACCGGACTTGCCACCCCCACCCAGCAAAGCCCCTATAAAAAAGGAG AACCAGTGGTTTGATGTGGGGGTCATTAAAGGTACCAGTGTAATGGTGACACACTATTTTCTGCCACCAG ATGATGCTGTTCAGTCAGATGATGACTCAGGCACCGTTCCAGACTATAACCAGCTAAAGAAGCAGGAGCT GCAGCCAGGCACAGCTTATAAATTTCGAGTTGCTGGAATCAATGCTTGTGGCCGGGGCCCCTTCAGTGAG ATCTCAGCCTTTAAGACTTGTCTGCCTGGTTTTCCAGGGGCTCCTTGTGCTATTAAAATCAGCAAGAGCC CAGATGGTGCTCACCTCACCTGGGAGCCACCCTCTGTGACCTCCGGCAAGATTATCGAGTACTCTGTGTA CCTGGCCATCCAGAGCTCACAGGCCAGTGGTGAGCCCAAGAGCTCTACCCCAGCCCAGCTGGCCTTCATG CGAGTATACTGTGGGCCTAGCCCTTCCTGCCTTGTGCAGTCCTCCAGCCTCTCCAACGCCCACATTGACT ATACTACAAAGCCTGCCATCATCTTCCGCATTGCTGCCCGCAATGAAAAGGGCTACGGCCCTGCCACACA AGTGAGGTGGTTACAAGAAACCAGTAAAGACAGCTCTGGCACCAAGCCAGCTAGCAAGCGGCCCATGTCG TCTCCAGAAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214974 representing NM_001139507 Red=Cloning site Green=Tags(s) MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWF IPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGH SFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTE KDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDD VKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQ VCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSV PANPPKSPAPAAAAPAVQPLTQVGITLVPQAAAAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVT GPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIP PSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTR PIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQ ASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIK TIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPG QPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPI TTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVS ILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVC DRQEAAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTA TTAMSSMGTGQQRDARRATNTPTVVRITVAPGALERAQGTVKPPCQTQQTNMTSTTMTVQATGAPCSAGP LLRPSVALETGSHSPAFVQLALPSVRVGLSGPSSKDVPTGRQPETYHTYTTNTPTTARSIMVAGELGTAR VVPTSQYDCLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPP CETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGALIPDATPSHGTLESGL EVVAVPTVTSQAGATLLASFSTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQG DSTNITSASAITTTVSSTLPRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTPLMGES AEVLSASQTPELQAAVDLSSTGDPSSVQEPTTSAVVATVVVQPPQPTQSEVDQLSLPQELMAEAQAGTTT LMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELGHLS AEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASA VPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPIKKE NQWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSE ISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFM RVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMS SPEM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001139507
ORF Size	6102 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001139507.1, NP_001132979.1
RefSeq Size	9128 bp
RefSeq ORF	6105 bp
Locus ID	363519
MW	209.2 kDa

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