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Zfyve27 (NM_199104) Rat Tagged ORF Clone
CAT＃: RR214883
- TrueORF®
Zfyve27 (Myc-DDK-tagged ORF) - Rat zinc finger, FYVE domain containing 27 (Zfyve27), (10 ug)
"NM_199104" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC72597 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214883 representing NM_199104
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCTTCGGATCGGGACCTGAGTGGACCAGAGGCGAGCCCCAGCGTGATGCCTGAGGTTCTCTCTG AGTGTTCACCTGCCCCTACCAAGTCAACAGCGTTTGATCTTTTCAACCTGGTTCTGTCCTACAAGAGGCT GGAGATCTACCTGGAACCCCTGAAGGATGCAGGTGACGGTGTTCGATACTTGCTAAGGTGGCAGATGCCT TTGTGTTCCTTGCTGACTTGCCTGGGCCTCAACATCTTGTTCCTCACTCTGAATGAGGGTGCATGGTACT CCGTGGGTGCCTTGATAATTTCGGTGCCTGCCCTACTGGGCTACCTTCAGGAGGTGTGCCGGGCACAGCT ACCAGAGTCTGAGCTGATGAGGCGGAAGTACCACAGCGTGCGGCAGGAAGACCTGCAGAGAGTTCGCCTT TCCCGCCCTGAGGCTGTAGCTGAGGTGAAAAGCTTCTTGATCCGACTGGAAGCCTTCTTGGCCCGCCTGT GCTATACCTGCGAGTCAGCTTACCGTGTACTTCACTGGGAGAACCCTGTGGTGTCCTCACAGTTCTATGG TGCTCTTCTGGGCATGGTTTGCATGCTCTACCTGCTGCCGCTCTGCTGGGTCCTCGCCCTTTTAAACAGC ACACTCTTTCTGGGAAATGGGGAATTCTTCCGAGTGGTGTCTGAGTACAGGGCTTGTCTGCAGCGGCGGA TGAGCCCCAAGCAGGAAGAGTGCGTCTGTGAGGGCTCGGCACTGCAGGATGCCGGGGGGAGGGCTGTTGT ACTGGACAGCACCCCTGCCCCTACACCCACAGAGGACCTCACGCCAGGCAGTGTGGAGGAAGCTGAGGAG GCTGAGCCAGATGAGGAGTTCAAAGATGCAATAGAGGAGGACGATGAGGGAACCCCATGCCCAGCAGAGG ATGAGCTGACCATGCAGGACAATGGCTTCCTCAGCAAGAATGAGGTACTGCGCAGCAAGGTGTCGAAGCT TACAGAGCGGCTCCGCAAGCGCTACCCGACCAACAACTTCGGGAACTGTGCAGGCTGTGCTGCCACCTTC TCCGTGCTGAAGAAGAGGCGAAGCTGCAGCAACTGTGGGAACAGCTTCTGCTCTCGGTGCTGCTCCTTCA AGGTGCCCAAGTCCTCCATGGGGGCCACAGCTCCTGAAGCCCAGAGAGAGACTGTGTTTGTGTGTGCCTC CTGTAATCAGACCTTGAGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214883 representing NM_199104
Red=Cloning site Green=Tags(s) MQSSDRDLSGPEASPSVMPEVLSECSPAPTKSTAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMP LCSLLTCLGLNILFLTLNEGAWYSVGALIISVPALLGYLQEVCRAQLPESELMRRKYHSVRQEDLQRVRL SRPEAVAEVKSFLIRLEAFLARLCYTCESAYRVLHWENPVVSSQFYGALLGMVCMLYLLPLCWVLALLNS TLFLGNGEFFRVVSEYRACLQRRMSPKQEECVCEGSALQDAGGRAVVLDSTPAPTPTEDLTPGSVEEAEE AEPDEEFKDAIEEDDEGTPCPAEDELTMQDNGFLSKNEVLRSKVSKLTERLRKRYPTNNFGNCAGCAATF SVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199104 |
| ORF Size | 1212 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199104.2, NP_954535.2 |
| RefSeq Size | 5418 bp |
| RefSeq ORF | 1215 bp |
| Locus ID | 309376 |
| UniProt ID | Q6P7B7 |
| MW | 44.9 kDa |
| Gene Summary | Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport (PubMed:17082457). Promotes axonal elongation and contributes to the establishment of neuronal cell polarity. Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner. Contributes to both the formation and stabilization of the tubular ER network. Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections. Acts as an adapter protein that facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214883 | Zfyve27 (untagged ORF) - Rat zinc finger, FYVE domain containing 27 (Zfyve27), (10 ug) |
CNY 3,990.00 |
|
| RR214883L3 | Lenti ORF clone of Zfyve27 (Myc-DDK-tagged ORF) - Rat zinc finger, FYVE domain containing 27 (Zfyve27), (10 ug) |
CNY 6,080.00 |
|
| RR214883L4 | Lenti ORF clone of Zfyve27 (mGFP-tagged ORF) - Rat zinc finger, FYVE domain containing 27 (Zfyve27), (10 ug) |
CNY 6,650.00 |
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