Cad (NM_001105710) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214754 representing NM_001105710 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCTGGTGTTGGAGGACGGGTCGGTGCTGCAGGGCCGGCCCTTCGGGGCCGCTGTGTCGACTG CTGGGGAAGTGGTGTTTCAGACCGGCATGGTCGGCTACCCGGAGGCCCTCACTGACCCTTCCTACAAAGC TCAGATCCTAGTGCTAACGTACCCTCTCATCGGCAACTACGGTATTCCCTCAGATGAAGAGGACGAGTTC GGCCTGAGCAAGTGGTTTGAATCCTCGGAGATCCATGTGGCAGGTCTGGTGGTTGGGGAATGCTGTCCCA CGCCCAGCCACTGGAGTGCCACCTGCACCCTGCATGAGTGGCTGCAACAGCATGGCATCCCCGGCCTGCA AGGAGTGGACACTCGGGAGCTGACTAAGAAGTTGCGGGAACAAGGGTCTCTTTTGGGGAAGCTGGTCCAG AAAGGGACAGAGCCTTCAGCCCTGCCATTTGTGGACCCCAATGCCAGGCCCCTGGCACCAGAGGTCTCTA TTAAGACCCCACGGGTATTCAATGCGGGGGGTGCCCCTCGGATCTTTGCTGTAGACTGTGGCCTCAAGTA CAATCAGATCCGATGTCTCTGCCAGCTTGGGGCTGAGGTTACCGTGGTACCCTGGAACCATGAATTAGAC AGTCGGAAATATGACGGTCTCTTCCTAAGTAATGGACCTGGTGATCCTGCCTCCTATCCTGGTGTGGTGT CCACACTGAATCGAGTCTTATCTGAGCCTAATCCCCGACCTGTGTTTGGAATCTGCCTTGGACACCAGCT ATTGGCTTTAGCCATTGGGGCCAAAACTTACAAGATGAGATACGGAAACCGAGGCCACAACCAGCCCTGT TTACTGGTGGGTACTGGGCGCTGCTTTCTGACCTCCCAGAATCACGGGTTTGCCGTGGACGCGGATTCGC TGCCAGCAGGCTGGGCTCCTCTATTTACCAACGCCAATGACTGTTCCAACGAAGGCATTGTACACGACAA TCTGCCCTTCTTCAGCGTCCAGTTTCACCCCGAGCACCGGGCTGGCCCTTCAGATATGGAGCTACTCTTC GATGTGTTTCTAGAAACCGTGAGAGATACTGTAGCTGGGAACGCCGAGGGCCGGACAGTAAGAGAACGTT TGGCTCAGCGCCTCTGTCCCCCTGAGCTTCCCATTCCTGGTTCTGGGCTCCCACCACCACGAAAAGTTCT GATCCTGGGCTCGGGGGGCCTCTCCATTGGCCAGGCTGGAGAATTTGACTACTCGGGCTCTCAGGCAATT AAAGCCCTAAAGGAGGAGAACATCCAAACATTACTGATCAACCCCAACATTGCCACAGTGCAGACTTCTC AGGGGCTGGCAGACAAGGTCTATTTCCTTCCCATAACACCTCACTACGTAACCCAGGTGATTCGTAATGA ACGCCCAGATGGTATATTACTGACTTTCGGGGGTCAGACAGCCCTGAACTGCGGTGTGGAGCTGACCAAA GCTGGGGTACTAGCTCGGTATGGGGTCCGGGTCTTGGGTACACCTGTGGAGACCATTGAACTGACTGAGG ACCGGCGAGCCTTTGCTGCCAGAATGGCTGAGATTGGAGAGCACGTTGCCCCCAGTGAAGCTGCAAATTC TCTTGAACAGGCTCAGGCAGCTGCCGAGCGGCTAGGCTACCCTGTGCTGGTGCGAGCAGCCTTTGCCCTG GGTGGTCTTGGTTCTGGCTTTGCTTCCACCAAAGAGGAACTCTCAGCTCTTGTGGCTCCAGCCTTTGCCC ATACCAGCCAGGTGCTGATAGACAAGTCTCTGAAGGGCTGGAAGGAGATTGAATATGAGGTGGTGAGAGA TGCCTATGGCAACTGCGTAACGGTATGTAACATGGAGAACTTAGACCCACTGGGCATCCATACGGGGGAG TCCATAGTGGTGGCTCCAAGCCAGACACTGAATGACAGAGAGTACCAGCTTCTGCGGCGGACAGCTATCA AGGTCACCCAGCACCTGGGGATTGTTGGGGAGTGCAATGTGCAGTATGCCTTGAACCCCGAGTCTGAGCA GTATTACATCATTGAAGTAAATGCCAGGCTCTCCCGAAGCTCTGCCCTGGCCAGTAAGGCCACAGGCTAT CCACTAGCCTATGTGGCAGCCAAGCTAGCATTGGGCATTCCCCTGCCAGAGCTCAGAAACTCTGTTACGG GGGGAACAGCAGCCTTTGAGCCCAGCCTGGACTACTGCGTGGTAAAGATTCCTCGCTGGGACCTCAGCAA GTTCCTGCGTGTCAGTACCAAGATTGGGAGCTGCATGAAGAGTGTTGGTGAAGTCATGGGCATCGGGCGT TCCTTTGAGGAGGCCTTCCAAAAGGCCCTGCGCATGGTAGACGAGAACTGTGTAGGCTTCGACCATACAG TGAAGCCAGTCAGCGACGTGGAGCTGGAGACGCCGACGGATAAGCGGATCTTTGTGGTGGCTGCTGCTCT GTGGGCTGGCTACTCTGTGGAGCGCCTCTACGAGCTCACCCGCATCGACTGCTGGTTCCTGCACCGGATG AAGCGCATTGTGACCCACGCCAAGTTGCTGGAACAACACCGTGGACGGCCCTTACCCCAAGACCTGCTGC ACCAGGCCAAGTGCCTCGGCTTCTCAGACAAGCAAATTGCTCTTGCAGTCCTGAGCACAGAGCTGGTTGT TCGGAAGCTGCGTCAGGAACTGGGGATCTGCCCAGCAGTGAAGCAGATCGACACAGTTGCAGCTGAGTGG CCAGCCCAGACAAATTACCTGTACCTGACATACTGGGGCAACACCCATGACCTCGACTTTCGGACACCTC ACGTCCTGGTCCTTGGCTCTGGCGTCTACCGGATCGGCTCCAGCGTTGAGTTTGATTGGTGTGCTGTGGG CTGCATCCAGCAGCTCCGGAAGATGGGTTATAAGACCATCATGGTGAACTACAACCCAGAGACAGTCAGC ACCGACTATGATATGTGTGACCGACTCTACTTTGATGAGATCTCTTTTGAGGTGGTGATGGACATCTATG AGTTAGAAAACCCCGAAGGTGTGATTCTGTCCATGGGCGGGCAGCTGCCCAACAACATGGCTATGGCTTT GCATCGGCAGCAGTGCCGAGTGCTGGGCACCTCCCCCGAAGCCATCGACTCAGCCGAGAACCGGTTCAAG TTCTCCCGGCTCCTAGATACCATTGGTATCAGCCAGCCTCAGTGGCGAGAGCTCAGTGACCTCGAGTCCG CTCGCCAGTTCTGCCAGACCGTTGGGTACCCCTGCGTAGTGCGTCCCTCCTATGTGCTGAGTGGTGCTGC TATGAACGTGGCCTACACTGATGGGGACCTGGAGCGCTTCCTGAGCAGTGCAGCTGCTGTCTCTAAGGAG CACCCTGTGGTCATCTCCAAATTCATTCAGGAGGCAAAGGAGATTGATGTAGATGCTGTGGCCTGTGACG GCATCGTGTCGGCTATTGCCATCTCTGAGCACGTGGAGAATGCAGGTGTGCATTCAGGGGATGCCACGCT GGTGACCCCCCCACAAGACATCACCCCCAAAACTCTGGAGCGGATCAAAGCCATTGTGCATGCTGTGGGC CAGGAGCTACAGGTCACGGGGCCCTTCAATCTGCAGCTCATTGCCAAGGACGACCAGCTGAAGGTCATTG AATGTAACGTGCGGGTGTCGCGCTCCTTCCCGTTCGTGTCCAAGACACTTGGTGTCGACCTCGTAGCCTT GGCCACAAGGATCATCATGGGGGAGAAAGTAGAGTCCGTGGGGCTCATGACTGGCTCTGGAGTCGTGGGA GTAAAGGTACCTCAGTTCTCCTTCTCACGCTTGGCCGGTGCTGACGTGGTCCTGGGTGTGGAGATGACCA GTACTGGAGAGGTGGCTGGCTTCGGAGAGAGCCGTTGTGAAGCCTACCTCAAAGCCATGCTCAGCACTGG CTTTAAGATCCCAAAGAAGAACATCCTGCTGACCATTGGCAGCTACAAGAATAAAAGTGAGCTGCTCCCA ACTGTGCGGTTGCTGGAGAGCCTGGGCTACAGTCTGTATGCCAGCTTGGGAACTGCCGACTTCTACACCG AGCACGGCGTCAAGGTAACAGCTGTGGACTGGCACTTTGAAGAGGCTGTGGATGGTGAGTGCCCACCACA ACGGAGCATCTTGGATCAGCTGGCCGAGAATCACTTTGAGCTGGTGATTAACCTGTCGATGCGCGGGGCT GGGGGTCGGCGTCTTTCCTCCTTCGTTACCAAGGGCTACCGCACCCGGCGTCTGGCCGCGGATTTCTCCG TGCCTCTCATCATTGATATCAAGTGCACCAAACTCTTCGTGGAGGCCCTAGGCCAGATCGGGCCAGCCCC TCCTTTGAAAGTCCACGTTGACTGCATGACCTCCCAGAAGCTAGTGCGCCTGCCTGGATTGATTGATGTC CACGTGCACCTTCGGGAACCAGGTGGGACTCACAAAGAGGACTTTGCCTCAGGCACAGCTGCCGCCTTGG CTGGGGGCGTCACCATGGTTTGTGCTATGCCTAATACCCGGCCCCCCATCATTGATGCCCCAGCTCTGGC GCTTGCCCAGAAGCTGGCAGAGGCTGGCGCCCGCTGTGACTTTACCCTCTTCCTCGGAGCCTCATCTGAA AATGCAGGGACTCTGGGTGCTGTGGCTGGGTCTGCAGCAGGCCTGAAGCTCTACCTCAATGAGACCTTCT CTGAGCTACGGCTGGACAGTGTGGCCCAGTGGATGGAGCATTTTGAAACCTGGCCTTCCCACCTCCCCAT TGTGGCCCACGCAGAGCGGCAGAGTGTCGCTGCGGTCCTCATGGTGGCTCAGCTGACACGGCGTTCAGTG CACATATGTCATGTGGCTCGGAAGGAAGAGATCCTGCTGATTAAAACTGCAAAGGCACAGGGGCTGCCAG TGACGTGTGAGGTCGCACCTCACCACCTGTTCCTAAACCGGGAAGACTTGGAGCGCCTGGGACCTGGGAA GGGAGAGGTCCGCCCTGAGCTGGGTTCCCGAGAGGATATGGAAGCCCTGTGGGAGAATATGGCGGTCATC GACTGTTTTGCCTCAGACCACGCTCCCCATACCTTGGAAGAGAAGTGTGGGCCTAAGCCTCCACCAGGCT TTCCAGGACTAGAGACCATGTTGCCGCTGCTACTGACAGCTGTGAGTGAAGGCCGCCTCAGTCTGGATGA CCTGCTACAGCGATTGCACCACAACCCTCGGCGGATCTTCCACCTGCCTCTCCAGGAGGACACCTATGTA GAGGTGGATCTGGAGCATGAGTGGACGGTCCCCAGCCACATGCCCTTCTCTAAAGCCCACTGGACCCCAT TTGAAGGTCAGAAGGTGAAGGGCACCGTGCGCCGTGTGGTCTTGAGAGGGGAGGTCGCCTATATTGATGG ACAGGTGCTGGTACCCCCGGGCTATGGACAAGATGTACGGAAGTGGCCTCAGGGGGCTGTTCCTCAGCCT CCTCCTCCAACTCCTACTACCACTGAAATAACCACGACACCTGAGAGACCACGCCGAGTCATCCCAGGCC TTCCTGATGGCCGATTCCACCTGCCACCTCGAATCCACCGGGCCTCTGACCCAGGGCTGCCAGCTGAGGA ACCCAAAGAGAAGCCACCCAGGAAGGTAGCAGAATCAGAGCTGATAGGAACCCCTGATGGCCCCTGCTAC CCTGTACCACCAGTACCTAGACAGGCATCACCACAGAATCTAGGATCTTCTGGCCTGCTGCACCCCCAGA TGTCACCTTTACTGCACTCCCTAGTGGGCCAACACATCCTGTCTGTCAAGCAGTTCACTAAGGATCAGAT GTCTCATCTGTTCAACGTGGCGCACACACTACGGATGATGGTGCAGAAAGAACGGAGCCTCGACATCCTA AAGGGGAAGGTCATGGCCTCTATGTTCTACGAGGTGAGCACCCGCACCAGTAGCTCCTTCGCAGCAGCCA TGGCCCGGCTCGGGGGTGCCGTTCTCAGCTTCTCTGAAGCCACGTCCTCCGTCCAGAAGGGCGAATCCCT CGCCGACTCGGTGCAGACCATGAGTTGCTATGCTGATGTTGTTGTACTCCGTCACCCTCAGCCTGGAGCT GTGGAGCTGGCGGCCAAGCACTGTCGAAGACCAGTGATCAATGCTGGAGATGGAGTTGGGGAGCACCCCA CTCAGGCCCTGCTGGACATCTTCACCATTCGAGAAGAGCTTGGGACTGTCAATGGCATGACGATCACTAT GGTAGGCGACCTGAAGCATGGACGCACAGTGCACTCCCTGGCCTGCCTGCTCACCCAGTACCGTGTGAGC CTACGCTACGTGGCACCTCCCAGCCTGCGCATGCCATCCAGCGTGTGGGACTTTGTGGCCTCCCGAGGCA CCAAGCAGGAGGAGTTCGAGAGCATCGAGGAGGCGCTGCCAGACACTGACGTGCTCTACATGACTCGGAT CCAGAAAGAACGATTTGGCTCTTCCCAGGAATATGAAGCTTGCTTTGGTCAGTTCATCCTCACTCCTCAC ATCATGACCCGGGCCAAGAAAAAGATGGTGGTGATGCACCCAATGCCTCGTGTCAATGAGATAAGCGTGG AGGTGGACTCGGACCCCAGAGCAGCCTATTTCCGCCAAGCTGAGAACGGCATGTACATCCGCATGGCTCT GCTTGCCACCGTGCTAGGCCGTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214754 representing NM_001105710 Red=Cloning site Green=Tags(s) MAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEF GLSKWFESSEIHVAGLVVGECCPTPSHWSATCTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQ KGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRIFAVDCGLKYNQIRCLCQLGAEVTVVPWNHELD SRKYDGLFLSNGPGDPASYPGVVSTLNRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPC LLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDNLPFFSVQFHPEHRAGPSDMELLF DVFLETVRDTVAGNAEGRTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAI KALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGILLTFGGQTALNCGVELTK AGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFAL GGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGE SIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGY PLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGR SFEEAFQKALRMVDENCVGFDHTVKPVSDVELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRM KRIVTHAKLLEQHRGRPLPQDLLHQAKCLGFSDKQIALAVLSTELVVRKLRQELGICPAVKQIDTVAAEW PAQTNYLYLTYWGNTHDLDFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVS TDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFK FSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKE HPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVG QELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVESVGLMTGSGVVG VKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLP TVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGA GGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDV HVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSE NAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPSHLPIVAHAERQSVAAVLMVAQLTRRSV HICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVI DCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYV EVDLEHEWTVPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQP PPPTPTTTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKPPRKVAESELIGTPDGPCY PVPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDIL KGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGA VELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVS LRYVAPPSLRMPSSVWDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSSQEYEACFGQFILTPH IMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001105710
ORF Size	6675 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105710.2, NP_001099180.2
RefSeq Size	7126 bp
RefSeq ORF	6678 bp
Locus ID	24240
MW	243.5 kDa
Gene Summary	important enzyme in the pathway of pyrimidine biosynthesis; may be regulated by the MAPK pathway [RGD, Feb 2006]

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