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Bcl2 (NM_016993) Rat Tagged ORF Clone
CAT＃: RR214182
- TrueORF®
Bcl2 (Myc-DDK-tagged ORF) - Rat B-cell CLL/lymphoma 2 (Bcl2), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_016993" in other vectors (3)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Bcl-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214182 representing NM_016993
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAAGCCGGGAGAACAGGGTATGATAACCGGGAGATCGTGATGAAGTACATCCATTATAAGCTGT CACAGAGGGGCTACGAGTGGGATACTGGAGATGAAGACTCCGCGCCCCTGAGGGCTGCCCCCACCCCTGG CATCTTCTCCTTCCAGCCTGAGAGCAACCGAACGCCCGCTGTGCACCGAGACACGGCTGCCAGGACGTCG CCTCTACGGCCCCTTGTCGCCAACGCTGGGCCTGCGCTCAGCCCTGTGCCACCTGTGGTCCACCTGACCC TCCGCCGGGCTGGGGATGACTTCTCTCGTCGCTACCGTCGCGACTTTGCAGAGATGTCCAGTCAGCTGCA CCTGACGCCCTTCACCGCGAGGGGACGCTTTGCCACGGTGGTGGAGGAACTCTTCAGGGATGGGGTGAAC TGGGGGAGGATTGTGGCCTTCTTTGAGTTCGGTGGGGTCATGTGTGTGGGGAGCGTCAACAGGGAGATGT CACCCCTGGTGGACAACATCGCTCTGTGGATGACTGAGTACCTGAACCGGCATCTGCACACCTGGATCCA GGATAACGGAGGCTGGGATGCCTTTGTGGAACTATATGGCCCCAGCATGCGACCTCTGTTTGATTTCTCC TGGCTGTCTCTGAAGACGCTGCTCAGCCTGGCCCTGGTGGGGGCCTGCATCACTCTGGGTGCATACCTGG GCCACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214182 representing NM_016993
Red=Cloning site Green=Tags(s) MAQAGRTGYDNREIVMKYIHYKLSQRGYEWDTGDEDSAPLRAAPTPGIFSFQPESNRTPAVHRDTAARTS PLRPLVANAGPALSPVPPVVHLTLRRAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVN WGRIVAFFEFGGVMCVGSVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFS WLSLKTLLSLALVGACITLGAYLGHK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016993 |
| ORF Size | 708 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016993.1, NP_058689.1 |
| RefSeq Size | 1179 bp |
| RefSeq ORF | 711 bp |
| Locus ID | 24224 |
| UniProt ID | P49950 |
| MW | 26.6 kDa |
| Gene Summary | an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD, Feb 2006] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A natural BH3 mimetic induces autophagy in apoptosis-resistant prostate cancer via modulating Bcl-2-Beclin1 interaction at endoplasmic reticulum
,null,
Cell Death and Differentiation
,PubMed ID 20577262
[Bcl2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214182 | Bcl2 (untagged ORF) - Rat B-cell CLL/lymphoma 2 (Bcl2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 3,990.00 |
|
| RR214182L3 | Lenti ORF clone of Bcl2 (Myc-DDK-tagged ORF) - Rat B-cell CLL/lymphoma 2 (Bcl2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,080.00 |
|
| RR214182L4 | Lenti ORF clone of Bcl2 (mGFP-tagged ORF) - Rat B-cell CLL/lymphoma 2 (Bcl2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,650.00 |
