Scn11a (NM_019265) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NaN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212224 representing NM_019265 Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGAGGAGAGGTACTACCCGGTGATCTTCCCGGACGAGCGGAATTTCCGCCCCTTCACTTCCGACTCTC TGGCTGCCATAGAGAAGCGGATTGCTATCCAAAAGGAGAGGAAGAAGTCCAAAGACAAGGCGGCAGCTGA GCCCCAGCCTCGGCCTCAGCTTGACCTAAAGGCCTCCAGGAAGTTACCTAAGCTTTATGGTGACATTCCC CCTGAGCTTGTAGCGAAGCCTCTGGAAGACCTGGACCCATTCTACAAAGACCATAAGACATTCATGGTGT TGAACAAGAAGAGAACAATTTATCGCTTCAGCGCCAAGCGGGCCTTGTTCATTCTGGGGCCTTTTAATCC CCTCAGAAGCTTAATGATTCGTATCTCTGTCCATTCAGTCTTTAGCATGTTCATCATCTGCACGGTGATC ATCAACTGTATGTTCATGGCGAATTCTATGGAGAGAAGTTTCGACAACGACATTCCCGAATACGTCTTCA TTGGGATTTATATTTTAGAAGCTGTGATTAAAATATTGGCAAGAGGCTTCATTGTGGATGAGTTTTCCTT CCTCCGAGATCCGTGGAACTGGCTGGACTTCATTGTCATTGGAACAGCGATCGCAACTTGTTTTCCGGGC AGCCAAGTCAATCTTTCAGCTCTTCGTACCTTCCGAGTGTTCAGAGCTCTGAAGGCGATTTCAGTTATCT CAGGTCTGAAGGTCATCGTAGGTGCCCTGCTGCGCTCGGTGAAGAAGCTGGTAGACGTGATGGTCCTCAC TCTCTTCTGCCTCAGCATCTTTGCCCTGGTCGGTCAGCAGCTGTTCATGGGAATTCTGAACCAGAAGTGT ATTAAGCACAACTGTGGCCCCAACCCTGCATCCAACAAGGATTGCTTTGAAAAGGAAAAAGATAGCGAAG ACTTCATAATGTGTGGTACCTGGCTCGGCAGCAGACCCTGTCCCAATGGTTCTACGTGCGATAAAACCAC ATTGAACCCAGACAATAATTATACAAAGTTTGACAACTTTGGCTGGTCCTTTCTCGCCATGTTCCGGGTT ATGACTCAAGACTCCTGGGAGAGGCTTTACCGACAGATCCTGCGGACCTCTGGGATCTACTTTGTCTTCT TCTTCGTGGTGGTCATCTTCCTGGGCTCCTTCTACCTGCTTAACCTAACCCTGGCTGTTGTCACCATGGC TTATGAAGAACAGAACAGAAATGTAGCTGCTGAGACAGAGGCCAAGGAGAAAATGTTTCAGGAAGCCCAG CAGCTGTTAAGGGAGGAGAAGGAGGCTCTGGTTGCCATGGGAATTGACAGAAGTTCCCTTAATTCCCTTC AAGCTTCATCCTTTTCCCCGAAGAAGAGGAAGTTTTTCGGTAGTAAGACAAGAAAGTCCTTCTTTATGAG AGGGTCCAAGACGGCCCAAGCCTCAGCGTCTGATTCAGAGGACGATGCCTCTAAAAATCCACAGCTCCTT GAGCAGACCAAACGACTGTCCCAGAACTTGCCAGTGGATCTCTTTGATGAGCACGTGGACCCCCTCCACA GGCAGAGAGCGCTGAGCGCTGTCAGTATCTTAACCATCACCATGCAGGAACAAGAAAAATTCCAGGAGCC TTGTTTCCCATGTGGGAAAAATTTGGCCTCTAAGTACCTGGTGTGGGACTGTAGCCCTCAGTGGCTGTGC ATAAAGAAGGTCCTGCGGACCATCATGACGGATCCCTTTACTGAGCTGGCCATCACCATCTGCATCATCA TCAATACCGTTTTCTTAGCCGTGGAGCACCACAACATGGATGACAACTTAAAGACCATACTGAAAATAGG AAACTGGGTTTTCACGGGAATTTTCATAGCGGAAATGTGTCTCAAGATCATCGCGCTCGACCCTTACCAC TACTTCCGGCACGGCTGGAATGTTTTTGACAGCATCGTGGCCCTCCTGAGTCTCGCTGATGTGCTCTACA ACACACTGTCTGATAACAATAGGTCTTTCTTGGCTTCCCTCAGAGTGCTGAGGGTCTTCAAGTTAGCCAA ATCCTGGCCCACGTTAAACACTCTCATTAAGATCATCGGCCACTCCGTGGGCGCGCTTGGAAACCTGACT GTGGTCCTGACTATCGTGGTCTTCATCTTTTCTGTGGTGGGCATGCGGCTCTTCGGCACCAAGTTTAACA AGACCGCCTACGCCACCCAGGAGCGGCCCAGGCGGCGCTGGCACATGGATAATTTCTACCACTCCTTCCT GGTGGTGTTCCGCATCCTCTGTGGGGAATGGATCGAGAACATGTGGGGCTGCATGCAGGATATGGACGGC TCCCCGTTGTGCATCATTGTCTTTGTCCTGATAATGGTGATCGGGAAGCTTGTGGTGCTTAACCTCTTCA TTGCCTTGCTGCTCAATTCCTTCAGCAATGAGGAGAAGGATGGGAGCCTGGAAGGAGAGACCAGGAAAAC CAAAGTGCAGCTAGCCCTGGATCGGTTCCGCCGGGCCTTCTCCTTCATGCTGCACGCTCTTCAGAGTTTT TGTTGCAAGAAATGCAGGAGGAAAAACTCGCCAAAGCCAAAAGAGACAACAGAAAGCTTTGCTGGTGAGA ATAAAGACTCAATCCTCCCGGATGCGAGGCCCTGGAAGGAGTATGATACAGACATGGCTTTGTACACTGG ACAGGCCGGGGCTCCGCTGGCCCCACTCGCAGAGGTAGAGGACGATGTGGAATATTGTGGTGAAGGCGGT GCCCTACCCACCTCACAACATAGTGCTGGAGTTCAGGCCGGTGACCTCCCTCCAGAGACCAAGCAGCTCA CTAGCCCGGATGACCAAGGGGTTGAAATGGAAGTATTTTCTGAAGAAGATCTGCATTTAAGCATACAGAG TCCTCGAAAGAAGTCTGACGCAGTGAGCATGCTCTCGGAATGCAGCACAATTGACCTGAATGATATCTTT AGAAATTTACAGAAAACAGTTTCCCCCAAAAAGCAGCCAGATAGATGCTTTCCCAAGGGCCTTAGTTGTC ACTTTCTATGCCACAAAACAGACAAGAGAAAGTCCCCCTGGGTCCTGTGGTGGAACATTCGGAAAACCTG CTACCAAATCGTGAAGCACAGCTGGTTTGAGAGTTTCATAATCTTTGTTATTCTGCTGAGCAGTGGAGCG CTGATATTTGAAGATGTCAATCTCCCCAGCCGGCCCCAAGTTGAGAAATTACTAAGGTGTACCGATAATA TTTTCACATTTATTTTCCTCCTGGAAATGATCCTGAAGTGGGTGGCCTTTGGATTCCGGAGGTATTTCAC CAGTGCCTGGTGCTGGCTTGATTTCCTCATTGTGGTGGTGTCTGTGCTCAGTCTCATGAATCTACCAAGC TTGAAGTCCTTCCGGACTCTGCGGGCCCTGAGACCTCTGCGGGCGCTGTCCCAGTTTGAAGGAATGAAGG TTGTCGTCTACGCCCTGATCAGCGCCATACCTGCCATTCTCAATGTCTTGCTGGTCTGCCTCATTTTCTG GCTCGTATTTTGTATCTTGGGAGTAAATTTATTTTCTGGGAAGTTTGGAAGGTGCATTAACGGGACAGAC ATAAATATGTATTTGGATTTTACCGAAGTTCCGAACCGAAGCCAATGTAACATTAGTAATTACTCGTGGA AGGTCCCGCAGGTCAACTTTGACAACGTGGGGAATGCCTATCTCGCCCTGCTGCAAGTGGCAACCTATAA GGGCTGGCTGGAAATCATGAATGCTGCTGTCGATTCCAGAGAGAAAGACGAGCAGCCGGACTTTGAGGCG AACCTCTACGCGTATCTCTACTTTGTGGTTTTTATCATCTTCGGCTCCTTCTTTACCCTGAACCTCTTTA TCGGTGTTATTATTGACAACTTCAATCAGCAGCAGAAAAAGTTAGGTGGCCAAGACATTTTTATGACAGA AGAACAGAAGAAATATTACAATGCAATGAAAAAGTTAGGAACCAAGAAACCTCAAAAGCCCATCCCAAGG CCCCTGAACAAATGTCAAGCCTTTGTGTTCGACCTGGTCACAAGCCAGGTCTTTGACGTCATCATTCTGG GTCTTATTGTCTTAAATATGATTATCATGATGGCTGAATCTGCCGACCAGCCCAAAGATGTGAAGAAAAC CTTTGATATCCTCAACATAGCCTTCGTGGTCATCTTTACCATAGAGTGTCTCATCAAAGTCTTTGCTTTG AGGCAACACTACTTCACCAATGGCTGGAACTTATTTGATTGTGTGGTCGTGGTTCTTTCTATCATTAGTA CCCTGGTTTCCCGCTTGGAGGACAGTGACATTTCTTTCCCGCCCACGCTCTTCAGAGTCGTCCGCTTGGC TCGGATTGGTCGAATCCTCAGGCTGGTCCGGGCTGCCCGGGGAATCAGGACCCTCCTCTTTGCTTTGATG ATGTCTCTCCCCTCTCTCTTCAACATCGGTCTGCTGCTCTTCCTGGTGATGTTCATTTACGCCATCTTTG GGATGAGCTGGTTTTCCAAAGTGAAGAAGGGCTCCGGGATCGACGACATCTTCAACTTCGAGACCTTTAC GGGCAGCATGCTGTGCCTCTTCCAGATAACCACTTCGGCTGGCTGGGATACCCTCCTCAACCCCATGCTG GAGGCAAAAGAACACTGCAACTCCTCCTCCCAAGACAGCTGTCAGCAGCCGCAGATAGCCGTCGTCTACT TCGTCAGTTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATCGCTGTGATCCTCGAGAACTT CAACACAGCCACGGAGGAGAGCGAGGACCCTCTGGGAGAGGACGACTTTGAAATCTTCTATGAGGTCTGG GAGAAGTTTGACCCCGAGGCGTCGCAGTTCATCCAGTATTCGGCCCTCTCTGACTTTGCGGACGCCCTGC CGGAGCCGTTGCGTGTGGCCAAGCCGAATAAGTTTCAGTTTCTAGTGATGGACTTGCCCATGGTGATGGG CGACCGCCTCCATTGCATGGATGTTCTCTTTGCTTTCACTACCAGGGTCCTCGGGGACTCCAGCGGCTTG GATACCATGAAAACCATGATGGAGGAGAAGTTTATGGAGGCCAACCCTTTTAAGAAGCTCTACGAGCCCA TAGTCACCACCACCAAGAGGAAGGAGGAGGAGCAAGGCGCCGCCGTCATCCAGAGGGCCTACCGGAAACA CATGGAGAAGATGGTCAAACTGAGGCTGAAGGACAGGTCAAGTTCATCGCACCAGGTGTTTTGCAATGGA GACTTGTCCAGCTTGGATGTGGCCAAGGTCAAGGTTCACAATGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212224 representing NM_019265 Red=Cloning site Green=Tags(s) MEERYYPVIFPDERNFRPFTSDSLAAIEKRIAIQKERKKSKDKAAAEPQPRPQLDLKASRKLPKLYGDIP PELVAKPLEDLDPFYKDHKTFMVLNKKRTIYRFSAKRALFILGPFNPLRSLMIRISVHSVFSMFIICTVI INCMFMANSMERSFDNDIPEYVFIGIYILEAVIKILARGFIVDEFSFLRDPWNWLDFIVIGTAIATCFPG SQVNLSALRTFRVFRALKAISVISGLKVIVGALLRSVKKLVDVMVLTLFCLSIFALVGQQLFMGILNQKC IKHNCGPNPASNKDCFEKEKDSEDFIMCGTWLGSRPCPNGSTCDKTTLNPDNNYTKFDNFGWSFLAMFRV MTQDSWERLYRQILRTSGIYFVFFFVVVIFLGSFYLLNLTLAVVTMAYEEQNRNVAAETEAKEKMFQEAQ QLLREEKEALVAMGIDRSSLNSLQASSFSPKKRKFFGSKTRKSFFMRGSKTAQASASDSEDDASKNPQLL EQTKRLSQNLPVDLFDEHVDPLHRQRALSAVSILTITMQEQEKFQEPCFPCGKNLASKYLVWDCSPQWLC IKKVLRTIMTDPFTELAITICIIINTVFLAVEHHNMDDNLKTILKIGNWVFTGIFIAEMCLKIIALDPYH YFRHGWNVFDSIVALLSLADVLYNTLSDNNRSFLASLRVLRVFKLAKSWPTLNTLIKIIGHSVGALGNLT VVLTIVVFIFSVVGMRLFGTKFNKTAYATQERPRRRWHMDNFYHSFLVVFRILCGEWIENMWGCMQDMDG SPLCIIVFVLIMVIGKLVVLNLFIALLLNSFSNEEKDGSLEGETRKTKVQLALDRFRRAFSFMLHALQSF CCKKCRRKNSPKPKETTESFAGENKDSILPDARPWKEYDTDMALYTGQAGAPLAPLAEVEDDVEYCGEGG ALPTSQHSAGVQAGDLPPETKQLTSPDDQGVEMEVFSEEDLHLSIQSPRKKSDAVSMLSECSTIDLNDIF RNLQKTVSPKKQPDRCFPKGLSCHFLCHKTDKRKSPWVLWWNIRKTCYQIVKHSWFESFIIFVILLSSGA LIFEDVNLPSRPQVEKLLRCTDNIFTFIFLLEMILKWVAFGFRRYFTSAWCWLDFLIVVVSVLSLMNLPS LKSFRTLRALRPLRALSQFEGMKVVVYALISAIPAILNVLLVCLIFWLVFCILGVNLFSGKFGRCINGTD INMYLDFTEVPNRSQCNISNYSWKVPQVNFDNVGNAYLALLQVATYKGWLEIMNAAVDSREKDEQPDFEA NLYAYLYFVVFIIFGSFFTLNLFIGVIIDNFNQQQKKLGGQDIFMTEEQKKYYNAMKKLGTKKPQKPIPR PLNKCQAFVFDLVTSQVFDVIILGLIVLNMIIMMAESADQPKDVKKTFDILNIAFVVIFTIECLIKVFAL RQHYFTNGWNLFDCVVVVLSIISTLVSRLEDSDISFPPTLFRVVRLARIGRILRLVRAARGIRTLLFALM MSLPSLFNIGLLLFLVMFIYAIFGMSWFSKVKKGSGIDDIFNFETFTGSMLCLFQITTSAGWDTLLNPML EAKEHCNSSSQDSCQQPQIAVVYFVSYIIISFLIVVNMYIAVILENFNTATEESEDPLGEDDFEIFYEVW EKFDPEASQFIQYSALSDFADALPEPLRVAKPNKFQFLVMDLPMVMGDRLHCMDVLFAFTTRVLGDSSGL DTMKTMMEEKFMEANPFKKLYEPIVTTTKRKEEEQGAAVIQRAYRKHMEKMVKLRLKDRSSSSHQVFCNG DLSSLDVAKVKVHND SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	AscI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019265
ORF Size	5295 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019265.2, NP_062138.1
RefSeq Size	5905 bp
RefSeq ORF	5298 bp
Locus ID	29701
UniProt ID	O88457
MW	201.8 kDa
Gene Summary	Na channel alpha-subunit; may be involved in generation of electrical activity in peripheral sensory neurons [RGD, Feb 2006]

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