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Eif4e (NM_053974) Rat Tagged ORF Clone
CAT＃: RR212121
- TrueORF®
Eif4e (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 4E (Eif4e), (10 ug)
"NM_053974" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC93265 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212121 representing NM_053974
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACTGTGGAACCGGAAACCACCCCTACCACTAATCCCCCACCTGCGGAAGAGGAAAAAACAGAGT CTAATCAGGAGGTTGCTAATCCAGAGCACTATATTAAACACCCTCTACAGAACAGGTGGGCACTCTGGTT TTTTAAAAATGATAAAAGCAAAACTTGGCAAGCAAACCTTCGGTTGATCTCTAAGTTTGATACTGTTGAA GACTTTTGGGCTCTATACAACCATATCCAGTTGTCTAGTAATTTAATGCCTGGCTGTGACTACTCACTTT TTAAGGATGGTATTGAGCCTATGTGGGAAGATGAGAAAAACAAACGGGGAGGACGATGGCTAATTACATT GAACAAGCAGCAGAGACGAAGTGACCTCGATCGCTTTTGGCTAGAGACACTGCTGTGCCTTATTGGAGAA TCTTTTGATGACTACAGTGATGACGTGTGTGGAGCTGTTGTCAATGTTAGAGCTAAAGGTGATAAGATAG CAATATGGACGACTGAATGTGAAAACAGAGATGCAGTCACACACATAGGGAGGGTATACAAGGAAAGGTT AGGACTTCCTCCGAAGATAGTGATTGGTTATCAGTCCCACGCAGACACAGCTACAAAGAGCGGCTCCACC ACTAAAAATAGGTTTGTTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212121 representing NM_053974
Red=Cloning site Green=Tags(s) MATVEPETTPTTNPPPAEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVE DFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGE SFDDYSDDVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGST TKNRFVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053974 |
| ORF Size | 651 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053974.2, NP_446426.1 |
| RefSeq Size | 2395 bp |
| RefSeq ORF | 654 bp |
| Locus ID | 117045 |
| UniProt ID | P63074 |
| MW | 25.1 kDa |
| Gene Summary | binds to the mRNA 7-methylguanosine cap and mediates mRNA binding to the 40S ribosome in the rate limiting step in translational initiation [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212121 | Eif4e (untagged ORF) - Rat eukaryotic translation initiation factor 4E (Eif4e), (10 ug) |
CNY 3,990.00 |
|
| RR212121L3 | Lenti ORF clone of Eif4e (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 4E (Eif4e), (10 ug) |
CNY 6,080.00 |
|
| RR212121L4 | Lenti ORF clone of Eif4e (mGFP-tagged ORF) - Rat eukaryotic translation initiation factor 4E (Eif4e), (10 ug) |
CNY 6,650.00 |
