Crls1 (NM_001014258) Rat Tagged ORF Clone

CAT#: RR208424

  • TrueORF®

Crls1 (Myc-DDK-tagged ORF) - Rat cardiolipin synthase 1 (Crls1), (10 ug)



  "NM_001014258" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms RGD1311037
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR208424 representing NM_001014258
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGCTTGGCGCGTGGCACGCGGCGCGTGGGGGTCCCTTCGCGTGGCCGTCCGGCCTCCAGGGGCGC
GGCTCGGCAGGGGCGGCTCCCGCAGGGCCCTGCTACCACCCGCGGCCTGCTGCCTGGGCTGCCTGGCCGA
GCGCTGGCGGCTGCGTCCGGCCGCGTTCGCCTTGCGGCTGCCAGGCACCAGCCCGCGGACCCACTGCTCC
GGCGCCGGGAAGGCAGCCCCGGAGCCCGCAGCCGGAGGAGATGCCGCCGCGCAGGCCCCTAGTGCCCGGT
GGGTCCGGGCGAGCGCAACCAGCTCGTATGAAAATCCATGGACAATCCCAAATTTATTGTCAATGACAAG
AATTGGCCTGGCCCCGGTGTTGGGCTATCTGATTCTTGAAGAAGATTTTAATGTTGCACTGGGTGTTTTT
GCTTTAGCTGGGCTAACGGATTTGTTGGATGGATTTATTGCTCGAAACTGGGCCAATCAAAAATCAGCTT
TGGGAAGTGCTCTTGATCCACTTGCTGATAAGGTTCTTATCAGCATCTTATATATTAGCCTGACCTATGC
AGATCTTATTCCAGTCCCACTCACTTACATGATAATTTCAAGAGATGTAATGTTGATCGCTGCTGTGTTT
TATGTCAGATACCGAACTCTGCCAACACCGCGAACACTAGCTAAGTACTTCAATCCTTGCTATGCCACTG
CTAGGTTAAAACCAACATTCATCAGCAAGGTCAATACAGCAGTCCAGTTAATTTTGGTGGCAGCTTCTCT
GGCAGCTCCAGTTTTCAATTATGCTGACAGCATTTATCTTCAGATACTATGGTGTTGTACAGCATTCACT
ACAGCTGCGTCCGCATACAGTTATTATCACTATGGTCGGAAAACTGTTCAGGTGATAAAGGGCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR208424 representing NM_001014258
Red=Cloning site Green=Tags(s)

MLAWRVARGAWGSLRVAVRPPGARLGRGGSRRALLPPAACCLGCLAERWRLRPAAFALRLPGTSPRTHCS
GAGKAAPEPAAGGDAAAQAPSARWVRASATSSYENPWTIPNLLSMTRIGLAPVLGYLILEEDFNVALGVF
ALAGLTDLLDGFIARNWANQKSALGSALDPLADKVLISILYISLTYADLIPVPLTYMIISRDVMLIAAVF
YVRYRTLPTPRTLAKYFNPCYATARLKPTFISKVNTAVQLILVAASLAAPVFNYADSIYLQILWCCTAFT
TAASAYSYYHYGRKTVQVIKGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001014258
ORF Size 906 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001014258.1, NP_001014280.1
RefSeq Size 1921 bp
RefSeq ORF 909 bp
Locus ID 366196
UniProt ID Q5U2V5
MW 32.6 kDa
Gene Summary Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol (PG). CL is a key phospholipid in mitochondrial membranes and plays important roles in maintaining the functional integrity and dynamics of mitochondria under both optimal and stress conditions.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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