Cdca8 (NM_001025050) Rat Tagged ORF Clone
CAT#: RR208073
- TrueORF®
Cdca8 (Myc-DDK-tagged ORF) - Rat cell division cycle associated 8 (Cdca8), (10 ug)
"NM_001025050" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208073 representing NM_001025050
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCTAAGAAACGCAGCAGCCGCGGGACCAGGACCAACACACTGCGGAGCCGGAAGCTCGCCTCCT TCCTAAAGGACTTCGACCGCGAGGTGCAAGTTCGAACCAAGCAAATTGAGTCCGACAGGCAGACCCTCCT CAAGGAGGTGGAAAATCTCTACAACATCGAGGTCCTTCGGCTCCCCAAGGCGCTGCAAGTCATGAAGTGG CTTGACTACTTTGCCCTAGGAGGAAACAGACAGGCCCTGGAAGAGGCAGCAACAGCTGATCGAGACATCA CAGAGATAAACAATTTAACAGCTGAAGCTATTCAGACACCTTTAAAATCTGTTAAAAAGCGAAAGGTAAT CGAGGTGGATGAAGCCATAAAGGAAGAGGAAGAAGATGAAGAAGAAGAAGGAGGAGGAGGAGGAGGAAGA AAGAGCCATAAGAATCTTCGATCTGCGAGAGTCAAAAGATGCCCTCCATCCAAGAAGAGAACCCAGTCTA TACAAGGAAGAAGCAGAAGCAAAAGGTTAAGCCATGACTTTGTGACCCCAGCTATGAGCAGGCTGGAGCC ATCTCTGGTGAAACCAACCCCAGGCATGACACCTAGGTTTGATTCCAGGGTCTTCAAAACTCCAGGTCTA CGAACCCCAGCGGCCAAGGAGCAGGTTTACAACATCTCTATCAACGGCAGCCCTCTCGCAGACAGCAAAG AGATCTCCCTCAGTGTGCCCATAGGTGGCGGTGCGAGCTTGCGGCTATTGGCCAGTGACTTGCAGAGGGT CGATATTGCTCAGCTGAATCCAGAGGCCCTGGGAAACATTAAGAAACTCTCGAGTCGCCTTGCTCAGATC TGCAGCAGCATCCGGACAGGCCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208073 representing NM_001025050
Red=Cloning site Green=Tags(s) MAPKKRSSRGTRTNTLRSRKLASFLKDFDREVQVRTKQIESDRQTLLKEVENLYNIEVLRLPKALQVMKW LDYFALGGNRQALEEAATADRDITEINNLTAEAIQTPLKSVKKRKVIEVDEAIKEEEEDEEEEGGGGGGR KSHKNLRSARVKRCPPSKKRTQSIQGRSRSKRLSHDFVTPAMSRLEPSLVKPTPGMTPRFDSRVFKTPGL RTPAAKEQVYNISINGSPLADSKEISLSVPIGGGASLRLLASDLQRVDIAQLNPEALGNIKKLSSRLAQI CSSIRTGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001025050 |
ORF Size | 864 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025050.1, NP_001020221.1 |
RefSeq Size | 1547 bp |
RefSeq ORF | 867 bp |
Locus ID | 500545 |
UniProt ID | Q6AXW0 |
MW | 32.1 kDa |
Gene Summary | Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208073 | Cdca8 (untagged ORF) - Rat cell division cycle associated 8 (Cdca8), (10 ug) |
CNY 3,990.00 |
|
RR208073L3 | Lenti ORF clone of Cdca8 (Myc-DDK-tagged ORF) - Rat cell division cycle associated 8 (Cdca8), (10 ug) |
CNY 6,080.00 |
|
RR208073L4 | Lenti ORF clone of Cdca8 (mGFP-tagged ORF) - Rat cell division cycle associated 8 (Cdca8), (10 ug) |
CNY 6,650.00 |