Camta2 (NM_001105801) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206506 representing NM_001105801 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATACCAAGGACACCACTGAGGTTGCTGAGAACAGCCACCACCTGAAGATCTTCCTCCCCAAGAAAC TGCTGGAGTGTCTTCCTCGATGCCCACTGCTGCCTCCAGAGCGGCTCCGATGGAATACAAATGAGGAGAT TGCATCCTACCTGATCACCTTTGAGAAACATGATGAGTGGCTGTCTTGTGCCCCAAAGACAAGGCCTCAA AATGGCTCCATTATCCTCTACAATCGTAAGAAGGTGAAATACCGGAAGGATGGTTACCTTTGGAAGAAGC GGAAAGATGGGAAGACTACCCGAGAAGACCACATGAAACTCAAGGTCCAGGGCATGGAGTGTCTCTATGG CTGCTACGTTCACTCTTCCATCGTCCCCACATTCCATCGGCGCTGCTATTGGCTGCTCCAGAACCCTGAC ATCGTCCTTGTGCACTACCTGAATGTCCCAGCCCTGGAGGATTGTGGAAAGGGCTGTAGCCCCATCTTTT GTTCCATTAGTAGCGACCGGCGAGAATGGCTGAAGTGGTCACGTGAGGAGCTATTGGGACAGCTGAAGCC CATGTTTCATGGCATCAAGTGGAGCTGTGGGAATGGGTTGGAGGAATTCTCTGTGGAACAGTTGGTGCAG CAGATCTTGGACACCCATCCAACCAAACCAGCACCCCGAACCCATGCTTGTCTCTGCAGTGGGGGCCTTG GTTCTGGGAGCCTTACCCACAAATGCAGTAGCACGAAACACCGCATCATCTCTCCTAAAGTGGAGCCTCG AGCTTTAACCTTGGCTTCTATCTCCCACTCCAAGCCCCCTGAACCTCCACCACTGATAGCTCCACTTCCT CCCGAGCTCCCCAAGGCACATACCTCCCCATCTTCCTCCTCTTCTTCCTCCTCCTCAGGATTTGCAGAAC CCCTAGAAATCAGACCTAGCCCTCCTACCTCTCGAGGGGGTTCATCTCGAGGAGGCACTGCCATTCTTCT CCTAACGGGACTAGAGCAGCGTGCTGGGGGCCTGACACCCACCAGACACTTGGCTTCACAGGCTGAGCCT AGACCTCCTGTGAGCTTGGCTGTGGTTGTAGGTTCTGAGCCTTCTGCCCCGCCAGCTCCTCCCAGCCCTG CCTTTGACCCTGATCGTTTTCTCAACAGTCCCCAAAGGGGCCAGACATATGGAGGGGGCCAAGGGGTGAA TCCGGACTTCCCTGAGGCAGAGGGCACTCACACTCCCTGCCCTGCCCTAGAACCTGCTGCTGCCTTGGAG CCCCAGGCCGCTGCACGGGGTCTCCCTCCACAGTCGGGAGCAAGTGGGAGAAGAGGAAACAAATTCTTTA TCCAAGATGATGATAGTGGGGAGGAACTCAAGGGTCCAGGAACAGTGCCACCTGTCCCTTCACCCCCTCC CTCATCCCCATCCTCTCCTGCTGCCTTGCAGCCGTCAGGCAGGGCTACAAGAGGAGAAACCTTGTTTGGA GGCTCTGCTGGCAGCACCAGTGAGCTAGAGCCCTTCAGTCTTTCATCATTCCCAGACCTTATGGGAGAAC TCATCACTGATGAAGCCCCAGGTGTCCCTGCCCCAAGCCCCCAGCTTTCTCCTGTGCTTAACACCATCAC AGACTTTTCCCCAGAGTGGTCCTACCCAGAGGGTGGGGTCAAGGTGCTCATCACAGGCCCTTGGACAGAG GCCGCAGAGCATTACTCCTGTGTCTTCGATCACATCGCAGTGCCGGCCTCCCTGGTCCAGCCTGGTGTCT TACGCTGTTACTGTCCTGCCCATGAGGTAGGGCTGGTGTCTTTGCAGGTAGCAGGGCGGGAAGGCCCACT CTCTGCTTCTGTGCTCTTTGAGTATCGAGCTCGCCGGTTCCTGTCTCTGCCTAGTACACAGCTTGACTGG TTGTCACTGGATGACAGCCAGTTCCGGATGTCCATCCTAGAGCGGCTGGAGCAGATGGAGAAGCGGATGG CAGAGATCGCAGCAGCTGGACAGGCACCTGGCCAGGGTCCAGAGGCTCCTCCAATTCAGGATGAAGGCCA GGGCCCTGGCTTCGAAGCACGGGTGGTGGTCTTGGTAGAGAGCATGATCCCACGGTCCACTTGGAGGGGT CCTGAACGTCTGATGCATGGAAGCCCCTTCCGGGGCATGAGCCTTCTGCATCTGGCTGCTGCACAGGGCT ACGCTCGGCTCATTGAGACTCTGAGCCAGTGGCGGAGTGTGGAAACGGGAAGCCTGGACTTAGAGCAAGA GGCTGACCCGCTCAATGTGGACCATTTCTCTTGCACCCCTCTGATGTGGGCGTGTGCTCTGGGACACTTA GAAGCAGCTGTGCTCCTTTTCTGTTGGAACCGACAAGCACTGAGCATTCCTGACTCTCTGGGCCGGCTAC CCCTATCTGTGGCTCATTCTCGGGGTCATGTGCGCCTTGCCCGCTGCCTTGAAGAACTACAGAGACAGGA GCTTTCAGTTGAGCACCCACTCGCTCTATCTCCACAGTCTTCGAGCCCAGACACTGGCCTGAGCAGCGTC TCTTCACCCTCGGAGCTGTCAGATGGTACTTTCTCTGTGACATCAGCTTACTCAAGTGCCCCAGATGGCA GTCCTCCCCCTGCTCCCATGCTGGCCTCTGAAATTTCTATGGAGACCATCCCAGGCCAGCTTTCTTTTGG TGCCCCTGAGACACCCTTACTCCTCATGGACTACGAAGCCACTAACTCCAAAGAATCTGCTCCCTCCCCT TCTGGCCTCCCTCTAGCCCAGGATGACAGGGCTGCTCCAGAGGATGCTGACAGCCCACCAGCTGTGGATG TGATCCCGGTGGACATGATCTCACTGGCCAAGCAGATCATCGAAGCCACACCGGAACGGATTAAACGAGA GGACTTCTCAGAGTTACCTGAAGCTGGAGCCTCACCAAGGGAGCACACAGGCACTGTGGGGCTCAGTGAG ACCATGTCCTGGCTAGCCAGTTACCTGGAGAATGTGGACCATTTCCCCAGCTCAGCCTTTCCTAGTGAAC TGCCCTTTGAACGAGGTCGCCCAGCTATCCCTCCAGCACCTTCCTGGGCAGAGTTCCTCTCTGCATCTAC CAGTGGCAAGATGGAAAGTGACTTTGCCCTCCTGACACTCTCAGATCATGAGCAGCGGGAACTGTATGAG GCAGCACGAGTCATCCAGACGGCTTTTCGAAAGTATAAGGGTCGGAGGTTGAAGGAACAGCAGGAGGTAG CCGCAGCGGTGATTCAGCGTTGTTATCGGAAGTACAAGCAGCTGACCTGGATTGCACTTAAGTTTGCACT CTATAAGAAAATGACTCAGGCAGCCATCCTGATTCAGAGCAAGTTCCGAAGCTACTATGAACAGAAGAGG TTTCAGCAGAGCCGCCGAGCAGCTGTGCTTATCCAGCAGCACTACCGCTCCTACCGCCGCAGGCCGGGGC CTCCTCACCGGCCCTCAGGCCCCCTTCCTTCTCGAAACAAGGGCACCTTTCTCACGAAGAAGCAGGACCA GGCAGCCCGGAAAATAATGAGGTTCCTGCGGCGCTGCCGACACAGAATGAGGGAACTGAAGCAGAACCAG GAGCTGGAGGGGCTGCCCCAGCCAGGACTGGCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206506 representing NM_001105801 Red=Cloning site Green=Tags(s) MNTKDTTEVAENSHHLKIFLPKKLLECLPRCPLLPPERLRWNTNEEIASYLITFEKHDEWLSCAPKTRPQ NGSIILYNRKKVKYRKDGYLWKKRKDGKTTREDHMKLKVQGMECLYGCYVHSSIVPTFHRRCYWLLQNPD IVLVHYLNVPALEDCGKGCSPIFCSISSDRREWLKWSREELLGQLKPMFHGIKWSCGNGLEEFSVEQLVQ QILDTHPTKPAPRTHACLCSGGLGSGSLTHKCSSTKHRIISPKVEPRALTLASISHSKPPEPPPLIAPLP PELPKAHTSPSSSSSSSSSGFAEPLEIRPSPPTSRGGSSRGGTAILLLTGLEQRAGGLTPTRHLASQAEP RPPVSLAVVVGSEPSAPPAPPSPAFDPDRFLNSPQRGQTYGGGQGVNPDFPEAEGTHTPCPALEPAAALE PQAAARGLPPQSGASGRRGNKFFIQDDDSGEELKGPGTVPPVPSPPPSSPSSPAALQPSGRATRGETLFG GSAGSTSELEPFSLSSFPDLMGELITDEAPGVPAPSPQLSPVLNTITDFSPEWSYPEGGVKVLITGPWTE AAEHYSCVFDHIAVPASLVQPGVLRCYCPAHEVGLVSLQVAGREGPLSASVLFEYRARRFLSLPSTQLDW LSLDDSQFRMSILERLEQMEKRMAEIAAAGQAPGQGPEAPPIQDEGQGPGFEARVVVLVESMIPRSTWRG PERLMHGSPFRGMSLLHLAAAQGYARLIETLSQWRSVETGSLDLEQEADPLNVDHFSCTPLMWACALGHL EAAVLLFCWNRQALSIPDSLGRLPLSVAHSRGHVRLARCLEELQRQELSVEHPLALSPQSSSPDTGLSSV SSPSELSDGTFSVTSAYSSAPDGSPPPAPMLASEISMETIPGQLSFGAPETPLLLMDYEATNSKESAPSP SGLPLAQDDRAAPEDADSPPAVDVIPVDMISLAKQIIEATPERIKREDFSELPEAGASPREHTGTVGLSE TMSWLASYLENVDHFPSSAFPSELPFERGRPAIPPAPSWAEFLSASTSGKMESDFALLTLSDHEQRELYE AARVIQTAFRKYKGRRLKEQQEVAAAVIQRCYRKYKQLTWIALKFALYKKMTQAAILIQSKFRSYYEQKR FQQSRRAAVLIQQHYRSYRRRPGPPHRPSGPLPSRNKGTFLTKKQDQAARKIMRFLRRCRHRMRELKQNQ ELEGLPQPGLAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001105801
ORF Size	3606 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105801.2, NP_001099271.2
RefSeq Size	4438 bp
RefSeq ORF	3609 bp
Locus ID	287462
MW	131.7 kDa

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