Usp19 (NM_001001516) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205835 representing NM_001001516 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCTGGCACCAGTGCTACAGGCCCAAGGAGGGGGCCTCCGGGATTGGAGGAGGCAACTAGTAAGA AGAAACAGAAGGATCGAGCAAACCAGGAAAGTAAGGATGGAGATCCTAGGAGAGTGTCCATGCCTCGAAA GGAGCCCACCAAAGATGAACTGTTGCTCGATTGGAGACAGAGTTCAGATAAGGTGGTTGTTAAGCTGCGC GTGGGAACAGGTCCCATATGTCTGGAGGAAGTAGATGCTGCTTTCACAGACACGGACTGTGTGGTGAGGC TTCCAGATGGTCGGCAGTGGGGTGGTGTCTTCTTTGCTAAAATACAAAGTTCTTGTACCAAAGTGCAGAC TCGCAAGGGTGGTCTTCTACAGTTGGCACTACCAAAGAAGGTGCCTCTTCTCACGTGGCCCTCTCTCCTG AAGAAACCTCTAGGGACCCAAGAACTGGTGCCAGGATTGCGGTGCCAGGAGAACGGACAAGAACTGTCTC CCATTGCCCTGGAGCCAGGTTCTGAGCCCCGCAGAGCTAAACAGGAAGCTCGAAACCAGAAGAGGGCCCA GGGCCGTGGTGAGGTAGGCTCAGGGGCTAGCCCTGGGGCACAGGCAGGGCCCAGTGCCAAGAGGGCTGTT CATCTCTGCAGAGGGCCAGAAGGTGAAGGGTCCATGGATGGGCCTGGCCCCCAGGGTGATGCCCCATCCT TCCTGTCCGACTCAGCTACGCAGGTTGAGGCTGAGGAGCAGCTCCATGTTCCACCAGTGAACCCTCAAAC AAGTCTCTTGGGCTCAGAGAAGAATTTAGCCCTTTTGACAGTAGAGAAGACAGTGTCCCCCAGGAGTGAC TCAGTCTCCCCAGTCATGATCCGGAACAGAGACCCTGAGAAAGATGACCATTTCAAAGAGGAGATGGCAG TTGGAGCTGATCCTGCAGCCTTAGCGGATGAACCAGAGTCTATGGTGAACCTGGCATTTGTCAAGAATGA TTCGTATGAGAAGGGGCCGGATTCAGTGGTGGTGCACGTGTATGTGAAGGAGAGCCGCAGGGATACCTCT CGAGTACTTTTCCGAGAGCAGGACTTCACGCTGATCTTCCAGACCAGGGATGGAAATTTTCTGAGGCTGC ATCCGGGCTGTGGGCCCCACACCATCTTCCGCTGGCAGGTGAAGCTCAGGAACTTGATTGAGCCAGAGCA ATGTACGTTTTGTTTCACGGCCTCTCGAATCGATATCTGCCTCCGGAAGCGGCAGAGTCAGCGCTGGGGG GGACTGGAGGCCCCTGCTACACGAGTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGTCCAACCCCTTTGG ATTCAACCCCTCCAGGAGGTGGCCCCCTCCCTCTGACTGGCCAGGAGGAAGCCAGGGCTGTAGAGAAGGA AAAACCCAAGGCTCGGTCAGAGGACTCGGGGCTGGATGGTGTGGTGGCTCGCACCCCCTTGGAGCATGTT ACCCCAAAGCCAGAACCGCACTTGGCCTCGCCCAAACCCACATGTATGGTGCCTCCAATGCCTCATAGTC CAGTGAGTGGAGATAGTGTGGAGGAGGATGAAGAGGAAGAGAAGAAGGTTTGTCTCCCAGGCTTTACTGG CCTCGTCAACTTAGGGAACACCTGCTTCATGAATAGTGTCATTCAGTCTCTGTCCAACACTCGGGAGCTT CGTGACTTCTTCCACGACCGATCCTTTGAGGCTGAGATTAACTACAATAACCCATTGGGGACTGGTGGGC GTCTGGCTATTGGCTTCGCTGTGCTGCTCCGGGCCCTGTGGAAGGGCACTCACCAAGCCTTTCAACCCTC CAAGCTAAAGGCCATTGTAGCAAGCAAGGCCAGCCAGTTTACAGGCTACGCACAGCATGATGCCCAAGAG TTCATGGCTTTCTTGTTGGATGGGCTGCATGAAGACCTGAATCGGATCCAAAACAAACCCTACACAGAGA CTGTGGACTCAGATGGGCGACCTGACGAGGTGGTGGCTGAAGAAGCATGGCAGCGGCACAAGATGAGGAA CGACTCATTCATTGTGGACCTGTTTCAGGGCCAGTACAAGTCAAAGCTGGTGTGCCCTGTGTGTGCCAAG GTCTCCATCACTTTTGACCCGTTCCTTTATCTGCCGGTACCCTTGCCACAAAAGCAAAAGGTTCTCCCCA TATATTATTTTGCCAGAGAGCCCCATAGCAAACCCATCAAGTTCCTGGTGAGCGTCAGCAAGGAGAACTC CAGCGCAAGCGAAGTTTTGGAGTCCCTCTCTCAGAGTGTCCACGTGAAGCCTGAGAGCCTGCGCCTGGCT GAGGTAATTAAGAATCGTTTCCACCGTGTTTTTTTGCCCTCCCACTCACTGGATGCTGTGTCGCCAACGG ACGTGCTCCTGTGCTTTGAGCTGCTATCCCCAGAATTGGCTAAGGAGAGGGTAGTGGTGCTAGAGGTGCA GCAGCGCCCCCAGGTACCCAGCATCCCTATCTCCAAGTGTGCAGCCTGCCAGCGGAAGCAGCAATCAGAA GATGAAAAACTGAAGCGTTGTACCCGTTGCTACCGTGTGGGCTACTGCAACCAGTTCTGTCAGAAAACCC ATTGGCCTGACCACAAAGGCCTCTGCCGCCCCGAGAACATTGGCTACCCCTTCCTGGTCAGTGTACCTGC TTCACGCCTCACTTATGCCCGTCTTGCTCAGCTACTAGAAGGTTATGCCCGGTACTCTGTGAGCGTATTC CAACCACCCTTCCAGCCTGGCCGAATGGCTTTGGAATCACAGAGCCCTGGCTGTACCACGTTGCTTTCAA CCAGCTCTCTGGAGGCTGGGGATAGTGAAAGAGAACCCATTCAGCCTTCTGAGCTCCAGTTGGTGACTCC TGTGGCTGAGGGGGATACCGGGGCTCACCGCATGTGGCCACCTGCTGATCGGGGTCCTGTGCCTAGCACC AGTGGAATTTCTTCCGAGATGCTGGCCAGTGGGCCTATGGAAGGGTGTTCCTTGCTTGCTGGTGAGAGGG TGTCTCGGCCTGAAGCTGCTGTGCCTGGGTACCAGCACTCACGTGAGTCTGTGAGCGCCCACACGCCCCA GTTCTTCATCTATAAAATTGATGCGTCAAGCCGTGAGCAGCGGCTGGAGGACAAAGGGGACACTCCACTG GAGCTAGGTGATGACTGTAGCCTGGCTCTGGTGTGGCGGAACAACGAACGCCTTCAGGAGTTTGTGCTGG TGGCCTCCAAGGAGCTGGAGTGTGCTGAAGACCCAGGCTCCGCTGGTGAGGCTGCCCGTGCTGGCCACTT CACCCTGGACCAGTGCCTCAATCTCTTTACACGGCCTGAAGTGCTGGCACCTGAGGAAGCCTGGTACTGC CCACAGTGCAAACAGCATCGAGAGGCCTCCAAGCAGCTGCTGCTGTGGCGCCTACCAAATGTGCTCATCG TGCAGCTCAAGCGTTTCTCCTTTCGTAGCTTCATTTGGCGTGACAAGATCAATGACTTGGTGGAGTTTCC AGTTCGGAACCTGGACTTGAGCAAGTTCTGTATTGGTCAGAAAGAGGAGCAGCTGCCTAGCTATGACCTG TATGCAGTCATCAACCACTACGGAGGCATGATCGGCGGCCACTACACTGCCTGTGCACGGCTGCCCAGTG ACCGTAGTAGCCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACGGTGACAACAGTAGACGA GAGCCAGGTGGTGACACGCTATGCCTATGTGCTCTTCTACCGTCGGCGGAACTCTCCTGTGGAGAGGCCC CCCAGGGCAGCTCACGCTGAACACCACCCAGACCTAGGCCCTGCAGCCGAGGCTGCTGCCAGCCAGGCTT CCCGGATTTGGCAGGAGCTCGAGGCCGAGGAGGAGATGGTGCCCGAGGGGCCTGGGCCTCTGGGTCCTTG GGGGCCCCAAGACTGGGTGGGGCCCCCGCCACGTGGCCCTACCACATCAGACGAGGGCTGCCTCCGATAC TTTGTCCTGGGTACCGTGGCGGCTTTGGTGGCCCTTGTGCTCAACGTATTCTATCCTCTGGTATCTCAGA GTCGCTGGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205835 representing NM_001001516 Red=Cloning site Green=Tags(s) MSAGTSATGPRRGPPGLEEATSKKKQKDRANQESKDGDPRRVSMPRKEPTKDELLLDWRQSSDKVVVKLR VGTGPICLEEVDAAFTDTDCVVRLPDGRQWGGVFFAKIQSSCTKVQTRKGGLLQLALPKKVPLLTWPSLL KKPLGTQELVPGLRCQENGQELSPIALEPGSEPRRAKQEARNQKRAQGRGEVGSGASPGAQAGPSAKRAV HLCRGPEGEGSMDGPGPQGDAPSFLSDSATQVEAEEQLHVPPVNPQTSLLGSEKNLALLTVEKTVSPRSD SVSPVMIRNRDPEKDDHFKEEMAVGADPAALADEPESMVNLAFVKNDSYEKGPDSVVVHVYVKESRRDTS RVLFREQDFTLIFQTRDGNFLRLHPGCGPHTIFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRWG GLEAPATRVGGAKVAVPTGPTPLDSTPPGGGPLPLTGQEEARAVEKEKPKARSEDSGLDGVVARTPLEHV TPKPEPHLASPKPTCMVPPMPHSPVSGDSVEEDEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSNTREL RDFFHDRSFEAEINYNNPLGTGGRLAIGFAVLLRALWKGTHQAFQPSKLKAIVASKASQFTGYAQHDAQE FMAFLLDGLHEDLNRIQNKPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPVCAK VSITFDPFLYLPVPLPQKQKVLPIYYFAREPHSKPIKFLVSVSKENSSASEVLESLSQSVHVKPESLRLA EVIKNRFHRVFLPSHSLDAVSPTDVLLCFELLSPELAKERVVVLEVQQRPQVPSIPISKCAACQRKQQSE DEKLKRCTRCYRVGYCNQFCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYSVSVF QPPFQPGRMALESQSPGCTTLLSTSSLEAGDSEREPIQPSELQLVTPVAEGDTGAHRMWPPADRGPVPST SGISSEMLASGPMEGCSLLAGERVSRPEAAVPGYQHSRESVSAHTPQFFIYKIDASSREQRLEDKGDTPL ELGDDCSLALVWRNNERLQEFVLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEEAWYC PQCKQHREASKQLLLWRLPNVLIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPSYDL YAVINHYGGMIGGHYTACARLPSDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSPVERP PRAAHAEHHPDLGPAAEAAASQASRIWQELEAEEEMVPEGPGPLGPWGPQDWVGPPPRGPTTSDEGCLRY FVLGTVAALVALVLNVFYPLVSQSRWR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001001516
ORF Size	4071 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001001516.2, NP_001001516.1
RefSeq Size	4292 bp
RefSeq ORF	4074 bp
Locus ID	361190
UniProt ID	Q6J1Y9
MW	150.3 kDa
Gene Summary	Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing thier ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked ubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]

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