Jag1 (NM_019147) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203938 representing NM_019147 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGTCCCCACGGACGCGCGGCCGGCCGGGGCGCCCCTTGAGCCTTCTGCTCGCCCTGCTCTGTGCCC TGCGAGCCAAGGTGTGCGGGGCCTCGGGTCAATTTGAGCTGGAGATCCTGTCCATGCAGAACGTGAACGG AGAGTTACAGAATGGGAACTGTTGCGGCGGAGCCCGGAACCCTGGCGACCGCAAGTGCACCCGCGACGAG TGTGACACGTACTTCAAAGTGTGCCTCAAGGAGTATCAGTCCCGCGTCACGGCCGGGGGACCCTGCAGCT TCGGCTCAGGGTCTACGCCTGTCATCGGGGGCAATACCTTCAATCTCAAGGCCAGCCGCGGCAACGACCG TAATCGCATCGTACTGCCTTTCAGTTTCGCCTGGCCGAGGTCCTACACTTTGCTGGTGGAGGCATGGGAT TCCAGTAATGACACTATTCAACCTGATAGCATAATTGAAAAGGCTTCTCACTCAGGCATGATAAACCCCA GCCGGCAGTGGCAGACACTGAAACAGAACACAGGGATTGCCCACTTTGAGTATCAGATCCGAGTGACCTG TGATGACCACTACTATGGCTTTGGCTGCAACAAGTTCTGTCGGCCCAGAGATGACTTCTTTGGACATTAT GCCTGCGACCAGAATGGCAACAAAACCTGCATGGAAGGCTGGATGGGTCCTGAGTGTAACAAAGCAATAT GCCGACAGGGCTGCAGTCCCAAGCATGGCTCCTGCAAACTCCCAGGTGACTGCAGGTGCCAGTATGGTTG GCAGGGCCTGTACTGTGATAAGTGCATCCCGCACCCAGGATGTGTCCACGGCACCTGCAATGAACCCTGG CAGTGCCTCTGTGAAACCAACTGGGGTGGACAGCTCTGTGACAAAGATCTGAATTACTGTGGGACTCATC AGCCCTGTCTCAACCGGGGAACATGTAGCAACACTGGCCCTGACAAATACCAGTGTTCCTGCCCCGAGGG CTACTCAGGACCGAACTGTGAAATTGCTGAGCATGCCTGCCTCTCTGATCCCTGCCACAACAGAGGCAGC TGCAAGGAGACCTCCTCGGGCTTTGAGTGTGAGTGTTCTCCAGGCTGGACTGGACCCACGTGTTCCACAA ACATCGATGACTGTTCTCCAAATAACTGTTCCCATGGAGGCACCTGCCAGGATCTAGTGAATGGATTCAA GTGCGTGTGCCCACCCCAGTGGACTGGCAAGACTTGTCAGTTAGATGCAAATGAGTGCGAGGCCAAGCCT TGTGTAAATGCCAGATCCTGTAAGAATCTGATTGCCAGCTACTACTGTGATTGTCTTCCTGGCTGGATGG GTCAGAATTGTGACATAAATATCAATGACTGCCTTGGCCAGTGTCAGAATGATGCCTCCTGTCGGGATTT GGTTAATGGTTATCGCTGTATCTGTCCACCTGGCTATGCGGGCGATCACTGTGAAAGAGACATCGATGAA TGTGCTAGCAACCCCTGCTTGAATGGGGGTCACTGTCAGAATGAAATTAACAGATTCCAGTGTCTGTGTC CCACTGGTTTCTCTGGAAACCTCTGTCAGCTGGACATCGATTACTGCGAGCCCAACCCTTGCCAGAATGG CGCCCAATGCTACAATCGTGCCAGTGACTATTTCTGCAAGTGCCCTGAGGACTACGAGGGCAAGAACTGC TCACACCTGAAAGACCACTGCCGCACCACTCCCTGTGAAGTGATTGACAGCTGCACCGTGGCCATGGCCT CCAACGATACTCCTGAAGGGGTGCGGTATATCTCTTCCAACGTCTGTGGTCCCCATGGGAAGTGCAAGAG CGAGTCAGGAGGCAAATTCACCTGTGACTGTAACAAAGGCTTCACCGGCACCTACTGCCATGAAAATATC AACGACTGTGAGGGCAACCCCTGTACGAACGGTGGCACGTGCATCGACGGCGTTAACTCCTACAAGTGTA TCTGTAGTGACGGCTGGGAGGGCGCGCACTGTGAGAACAACATAAATGACTGTAGCCAGAACCCTTGCCA CTATGGGGGTACATGTCGAGACCTGGTCAATGACTTTTACTGTGACTGCAAAAATGGGTGGAAAGGGAAA ACCTGCCACTCGCGTGACAGCCAGTGTGACGAAGCCACGTGTAATAACGGTGGTACCTGCTATGATGAAG TGGATACGTTTAAGTGCATGTGTCCTGGTGGCTGGGAAGGAACAACCTGTAATATAGCTAGAAACAGTAG CTGCCTGCCGAACCCCTGTCATAATGGAGGCACCTGCGTGGTCAATGGAGACTCCTTCACCTGTGTCTGC AAAGAAGGCTGGGAGGGGCCGATTTGTACTCAAAATACCAACGACTGCAGTCCTCACCCTTGTTACAACA GCGGAACCTGTGTGGATGGAGATAACTGGTACCGGTGCGAATGTGCCCCTGGTTTTGCTGGGCCAGACTG CAGGATAAACATCAATGAGTGTCAGTCTTCCCCTTGTGCCTTTGGGGCCACCTGTGTGGATGAGATCAAT GGCTACCAGTGTATCTGCCCTCCAGGACATAGTGGTGCCAAGTGCCATGAAGTTTCAGGGAGATCTTGCA TCACCATGGGGAGAGTGATACTTGATGGGGCCAAGTGGGATGACGACTGTAACACCTGCCAGTGCCTGAA TGGACGGGTGGCCTGCTCCAAGGTCTGGTGTGGCCCGAGACCTTGCCGGCTCCACAAAGGGCACGGCGAG TGTCCCAATGGGCAGAGCTGCATCCCAGTCCTGGATGACCAGTGTTTCGTGCGCCCCTGCACTGGTGCTG GCGAGTGTCGGTCCTCCAGCCTCCAGCCAGTGAAGACCAAGTGTACATCGGACTCTTATTACCAGGACAA TTGTGCGAACATCACTTTCACCTTTAACAAGGAGATGATGTCTCCAGGTCTCACCACTGAGCACATTTGC AGCGAATTGAGGAATCTGAATATTCTGAAGAATGTTTCTGCTGAGTATTCAATCTACATAGCCTGTGAGC CTTCCTTATCAGCAAACAATGAAATACACGTGGCCATTTCTGCCGAAGACATCCGGGATGATGGAAACCC TGTCAAGGAAATTACTGATAAAATAATAGATCTCGTTAGTAAACGGGATGGGAACAGCTCACTTATCGCT GCTGTTGCAGAAGTCAGAGTTCAGAGGCGCCCTCTGAAAAACAGAACAGATTTCCTGGTTCCTCTGCTGA GCTCTGTCTTAACAGTGGCTTGGGTCTGTTGCTTGGTGACAGCCTTCTACTGGTGTGTGCGGAAGCGGCG GCGGAAGCCCAGCAGCCACACCCACTCTGCCCCTGAGGACAACACCACCAACAATGTGCGCGAACAGCTG AACCAAATCAAAAACCCCATCGAGAAACACGGAGCCAACACAGTCCCCATCAAGGATTATGAGAACAAAA ACTCCAAAATGTCAAAAATCAGGACACACAACTCGGAAGTGGAAGAGGATGATATGGATAAGCACCAGCA GAAAGTCCGGTTTGCCAAACAGCCCGTGTACACGCTGGTAGACAGAGAGGAGAAGGTCCCCCAGCGCACG CCGACAAAACACCCGAACTGGACAAACAAACAGGACAACAGAGACTTGGAAAGTGCCCAGAGCTTGAACC GGATGGAGTACATCGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203938 representing NM_019147 Red=Cloning site Green=Tags(s) MRSPRTRGRPGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDE CDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWD SSNDTIQPDSIIEKASHSGMINPSRQWQTLKQNTGIAHFEYQIRVTCDDHYYGFGCNKFCRPRDDFFGHY ACDQNGNKTCMEGWMGPECNKAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGTCNEPW QCLCETNWGGQLCDKDLNYCGTHQPCLNRGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGS CKETSSGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKP CVNARSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDE CASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNC SHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSESGGKFTCDCNKGFTGTYCHENI NDCEGNPCTNGGTCIDGVNSYKCICSDGWEGAHCENNINDCSQNPCHYGGTCRDLVNDFYCDCKNGWKGK TCHSRDSQCDEATCNNGGTCYDEVDTFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGDSFTCVC KEGWEGPICTQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEIN GYQCICPPGHSGAKCHEVSGRSCITMGRVILDGAKWDDDCNTCQCLNGRVACSKVWCGPRPCRLHKGHGE CPNGQSCIPVLDDQCFVRPCTGAGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHIC SELRNLNILKNVSAEYSIYIACEPSLSANNEIHVAISAEDIRDDGNPVKEITDKIIDLVSKRDGNSSLIA AVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWVCCLVTAFYWCVRKRRRKPSSHTHSAPEDNTTNNVREQL NQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKVRFAKQPVYTLVDREEKVPQRT PTKHPNWTNKQDNRDLESAQSLNRMEYIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019147
ORF Size	3657 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019147.1, NP_062020.1
RefSeq Size	5575 bp
RefSeq ORF	3660 bp
Locus ID	29146
UniProt ID	Q63722
MW	134.3 kDa
Gene Summary	ligand responsible for activating Notch1 [RGD, Feb 2006]

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