Rasgrf2 (NM_053721) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202861 representing NM_053721 Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGCAGAAGAGCGTGCGCTACAACGAGGGGCACGCCCTGTACCTGGCCTTCCTGGCGCGCAAGGAGGGCA CCAAGCGCGGTTTCCTGAGCAAAAAGGCTGCCGAGGCGAGCCGCTGGCACGAGAAGTGGTTCGCTCTCTA TCAGAATGTGCTCTTTTACTTCGAAGGCGAACAGAGCGGCCGCCCAGCGGGCATGTACCTCCTAGAGGGC TGCAGCTGTGAGCGGACGCCCGCCCCACCAAGGACCAACGCAGGACCCGCAGGCGCCCGGGACGCGCTGG ACAAACAGTATTACTTCACTGTCCTTTTTGGTCACGAAGGTCAGAAACCACTGGAGCTGCGCTGTGAGGA GGAGCAAGCAGGGAAAGAGTGGATGGAGGCCATTCACCAAGCCAGTTACGCAGACATTTTGATTGAGAGG GAAGTGTTAATGCAAAAGTACATTCATCTAGTTCAGATTGTGGAGACAGAAAAAATTGCTGCTAACCAAC TCCGACATCAACTCGAAGATCAAGATACCGAAATTGAAAGACTTAAATCAGAGATTGTTGCTCTTAATAA GACCAAGGAACGGATGCGCCCGTATCATACCCATCAAGAGGAGGAGGATCCAGACATCAAGAAGATCAAG AAGGTTCAGAGCTTCATGAGAGGGTGGCTGTGCAGGAGGAAGTGGAAGACCATCGTTCAGGACTACATTT GTTCCCCCCATGCTGAGAGCATGAGGAAGAGGAACCAGATTGTCTTCACGATGGTGGAGGCCGAGTCAGA ATATGTGCACCAGCTCTACATCCTGGTGAACGGTTTCCTCAGGCCTCTGCGCATGGCAGCCAGCTCCAAG AAGCCCCCCATTAGCCATGATGATGTCAGCAGCATTTTCCTCAACAGTGAAACAATCATGTTTCTTCATG AAATATTCCATCAAGGTCTAAAGGCAAGGCTGGCAAATTGGCCCACTTTGGTTTTAGCTGATCTCTTTGA TATTTTGCTGCCTATGCTCAACATTTATCAAGAATTTGTGCGTAATCATCAGTACAGCCTCCAAGTACTT GCCAACTGTAAGCAAAATAGAGATTTTGACAAGCTCTTAAAGCAGTATGAAGGCAACCCTGCCTGTGAGG GAAGGATGCTGGAGACATTCTTGACTTACCCAATGTTTCAGATCCCCAGGTATATCATCACGCTGCACGA ACTCCTGGCTCACACCCCCCATGAACACGTGGAGAGGAAAAGTCTGGAGTTCGCTAAGTCAAAGCTGGAG GAACTGTCCAGAGTGATGCACGACGAAGTCAGTGACACCGAAAACATACGGAAAAATCTTGCCATTGAGA GGATGATCGTGGAGGGCTGCGACATTTTGCTAGACACCAGTCAAACTTTCATCCGACAAGGTTCTCTTAT CCAAGTACCTTCTGTGGAGAGGGGGAAACTCAGTAAAGTCCGCCTGGGCTCCCTGTCTTTGAAAAAGGAA GGCGAGAGACAATGTTTCTTATTCACGAAGCACTTTCTGATCTGTACCCGAAGTTCAGGAGGAAAGCTTC ATTTGCTCAAGACAGGTGGGGTTCTCTCCCTCATTCAGTGTACGTTGATTGAGGAGCCGGACACAAGTGA TGATGACACCAAAGGTCCTGGTCACATGTTCGGACATCTGGATTTTAAAATCGTCGTGGAGCCTCCTGAT GCTGCCCCGTTCACTGTGGTCTTGCTAGCACCTTCGCGCCAGGAGAAGGCTGCCTGGATGAGCGACATCA GCCAGTGCGTGGACAATATCCGATGCAATGGATTGATGACTATAGTCTTCGAAGAGAATTCTAAAGTTAC TGTGCCCCATATGATAAAGTCTGATGCCCGTCTTCATAAAGACGACACTGACATCTGTTTCAGTAAAACA CTCAATTCCTGCAAAGTGCCCCAGATCCGCTACGCCAGCGTGGAGCGCCTCTTGGAGCGGTTGACGGATT TGCGGTTTCTGAGCATCGATTTCCTCAACACCTTCCTGCACACCTACCGCATTTTCACCACGGCTGCCGT AGTGCTGGGGAAGCTCTCCGACATCTACAAGAGGCCTTTCACCTCCATTCCTGTCAGATCACTAGAGTTA TTTTTTGCCACGAGCCAGAACAACAGAGAACATTTGGTCGATGGAAAATCCCCACGTCTTTGTCGCAAGT TCTCTTCCCCACCTCCCCTGGCTGTATCCAGAACGTCCTCCCCAGTGAGGGCCAGAAAGCTGTCTTTGAC ATCTTCCTTGAACTCAAGGATCGGAGCCTTGGACCTGACTACATCCTCATCTTCCAGTAGTCCCACCACC ACTGTCCACAGTCCTGCTGCGTCCCCACCACCTCACACTGCTGTCCCAGAGTCTGCACCAGCCGACCGAG CAGGAGACTCCACAGACATGTCACCTTGTAGATCTCCCTCAACCACTCCCCGGCACCTCCGATATCGCCA GCCTGGAGGACAGGTAGCTGACAGCACCCACTGTGCTGTTTCACCTGCTTCTGCTTTTGCGATCGCCACA GCAGCAGCAGGACATGGGAGTCCGCCAGGATTTAACAACGAGAGGACATGTGACAAGGAGTTCATCATAC GGAGAACAGCCACCAATCGAGTCCTGAATGTCCTCCGCCACTGGGTTTCAAAGCACTCACAGGATTTTGA ACTCAATAATGAACTCAAAATGAATGTTCTGAACTTGCTAGAAGAAGTTCTACGAGACCCGGACCTTCTT CCTCAAGAAAGGAAAGCCACGGCGAACATCCTGAGGGCTCTTTCACAAGATGATCAAGATGACATCCACC TAAAATTAGAGGATATAATTCAAATGACAGACTGTCCAAAGGCCGAGTGCTTTGAGACATTGTCAGCCAT GGAGCTGGCTGAGCAGATCACTCTCCTGGACCACATTGTTTTCAGAAGCATCCCCTATGAAGAGTTTCTT GGGCAGGGCTGGATGAAGCTGGATAAAAACGAAAGGACTCCTTATATCATGAAAACCAGCCAGCACTTCA ATGAGATGAGTAACCTGGTGGCCTCCCAGATAATGAATTATGCTGACATCAGTTCCCGTGCCAATGCCAT TGAGAAGTGGGTAGCAGTGGCGGACATCTGCCGATGTCTACACAACTACAATGGTGTGCTGGAGATCACC TCGGCCTTAAACAGAAGTGCCATCTACAGGCTGAAGAAAACCTGGACCAAGGTGTCCAAGCAGACAAAAG CTCTGATGGACAAACTTCAGAAGACTGTCTCGTCTGAAGGAAGATTTAAAAACCTCAGAGAAACTCTCAA AAACTGTAACCCCCCAGCTGTCCCTTACCTTGGGATGTACTTGACAGACCTGGCATTCATTGAAGAAGGG ACACCGAACTTTACCGAAGAAGGCCTTGTCAATTTCTCCAAAATGCGAATGATATCACACATCATTCGTG AGATACGCCAGTTCCAGCAGACCGCTTACCGCATAGACCAGCAGCCAAAGGTCATACAATACCTGCTTGA CAAAGCCCTTGTCATAGATGAAGATACACTCTATGAGCTGTCACTCAAAATTGAACCTCGACTCCCTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202861 representing NM_053721 Red=Cloning site Green=Tags(s) MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKAAEASRWHEKWFALYQNVLFYFEGEQSGRPAGMYLLEG CSCERTPAPPRTNAGPAGARDALDKQYYFTVLFGHEGQKPLELRCEEEQAGKEWMEAIHQASYADILIER EVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIVALNKTKERMRPYHTHQEEEDPDIKKIK KVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSK KPPISHDDVSSIFLNSETIMFLHEIFHQGLKARLANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVL ANCKQNRDFDKLLKQYEGNPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLE ELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKE GERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIQCTLIEEPDTSDDDTKGPGHMFGHLDFKIVVEPPD AAPFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKT LNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLEL FFATSQNNREHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSSLNSRIGALDLTTSSSSSSPTT TVHSPAASPPPHTAVPESAPADRAGDSTDMSPCRSPSTTPRHLRYRQPGGQVADSTHCAVSPASAFAIAT AAAGHGSPPGFNNERTCDKEFIIRRTATNRVLNVLRHWVSKHSQDFELNNELKMNVLNLLEEVLRDPDLL PQERKATANILRALSQDDQDDIHLKLEDIIQMTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFL GQGWMKLDKNERTPYIMKTSQHFNEMSNLVASQIMNYADISSRANAIEKWVAVADICRCLHNYNGVLEIT SALNRSAIYRLKKTWTKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEG TPNFTEEGLVNFSKMRMISHIIREIRQFQQTAYRIDQQPKVIQYLLDKALVIDEDTLYELSLKIEPRLPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	AscI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053721
ORF Size	3570 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053721.1, NP_446173.1
RefSeq Size	3573 bp
RefSeq ORF	3573 bp
Locus ID	114513
UniProt ID	Q99JE4
MW	135.8 kDa
Gene Summary	human homolog is involved in stimulating the conversion of the GDP-bound form of Ras into the active form [RGD, Feb 2006]

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