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Polr1b (NM_031773) Rat Tagged ORF Clone
CAT＃: RR202655
- TrueORF®
Polr1b (Myc-DDK-tagged ORF) - Rat polymerase (RNA) I polypeptide B (Polr1b), (10 ug)
"NM_031773" in other vectors (3)
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CNY 18,340.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RPA2; Rpo1-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202655 representing NM_031773
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTCGACAGCCGGTGGCGGAACCTGCCCAGCGGGCCCAGCCTAAAGCATTTAACCGACCCCTCGT ACGCGGTTCCTCCGGAGCAGCAAAAGGCGGCGTTGCAGGACCTGACGCGGGCGCACGTGGACTCCTTCAA CTACGCAGTGCTGGAGGGGCTGAGCCACGCGGTGCAGGCCATACCTCCCTTCGAATTTGCTTTCAAAGAT GAGCGCATATCTCTTACTATTGTGGACGCTGCCATCAGTCCACCGGCAGTGCCCAAAGGGACCATCTGCA AAGAGCTCAACATTTATCCAGCTGAGTGCCGTGGCCGGAGGAGCACGTACCGAGGGAAGCTGACGGCTGA TATCAGCTGGGCCGTGAATGGAGTCCCCAAAGGCATCATTAAACAATTTCTTGGCWATGTTCCCATCATG GTGAAGTCCAAGCTTTGCAACTTATACAACCTTCCTCCTCAAGTCCTCATTGAGCACCACGAGGAGGCAG AGGAAATGGGCGGTTATTTTATAATCAACGGCATTGAGAAAGTCATCCGCATGTTGATTATGCCTCGGAG AAATTTTCCCATCGCAATGATAAGACCGAAATGGAAAAGCAGAGGGCTCGGCTACACTCAGTTCGGGGTT TCCATTCACTGTGTGAGAGAGGAGCACTCTGCTGTCAATATGAACCTTCACTATGTGGAGAACGGCACGG TCATGTTAAACTTTATTTACCGCAAAGAGCTGTTTTTCCTTCCTTTGGGATTTGCACTTAAGGCACTTGT GAGCTTTTCTGACTATCAGATTTTCCAGGAGCTCATCAAAGGCAAAGAGGAGGACTCTTTCTTTAAGAAT TCTGTTTCTCAGATGCTGAGGATTGTAATGGAGGAGGGTTGTCACACACAGAAGCAGGTCCTCGACTATC TGGGCGAACGCTTCAGAGTAAAGCTCAGTCTTCCCGATTGGTACCCTAATGCGGAAGCTGCCGAGTTCCT GTTTAACCAGTGTATCTGCATCCACTTGAAATCCAACACTGACAAGTTTTACCTGCTCTGTCTCATGACC CGGAAGCTCTTTGCTTTAGCCAGAGGAGAGTGCATGGAGGACAATCCTGACAGTTTAGTGAATCAAGAAG TCCTTACCCCTGGGCAGCTCTTCCTGATGTTTCTGAAGGAAAAGATGGAGAATTGGCTACTGTCTATTAA AATAGCTTTAGATAAAAGGGCTCAGAAGACCAATGTTTCCATAAACAATGAAAATTTGATGAAGATTTTT AGTATGGGAACAGAGCTAACAAGACCATTTGAATATCTTCTTGCTACTGGAAATCTGCGTTCTAAAACAG GTCTTGGCTTCATGCAGGATTCTGGCCTGTGTGTTGTGGCTGACAAGCTGAACTTCATTCGCTATCTCTC CCATTTCCGCTGTGTGCACAGAGGGGCTGACTTTGCCAAGATGAGGACCACCACCGTGCGCAAGCTGCTG CCAGAATCCTGGGGCTTCCTCTGCCCTGTGCACACCCCAGACGGGGCACCGTGTGGGCTGCTGAACCACC TGACTGCTGTGTGTGAGGTTGTTACCAAGTTTGTGTACACAGCATCTATTCCAGCCTTGCTCTGTGGCTT AGGAGTCACTCCTGTTGATGCAGCACCATGTCGACCGTATAGTGACTGCTACCCTGTCCTGCTGGATGGC GTCATGGTGGGCTGGGTGGATAAGGAGCTGGCTCCTGAAGTGGCAGACACTCTCCGTCGATTTAAGGTGT TGAGAGAAAGGAGATGTTCCTCCCTGGATGGAGGTGGCCCTGATTCCCATGACAGGAAAGCCAAGCCTGT ACCCAGGGCTGTTCCTCTTCACCACTCCCTGCAGGCTGGTGAGGCCTGTGCAGAACCTGGAGCTGGGCAA AGAAGAGCTCGTTGGAACTATGGAGCAGCTCTTCATGAACATTGCCATCTTCGAGGACGAGGTTTTTGGT GGAGTTTCCACACACCAGGAGCTCTTCCCTCACAGCCTGCTGAGGTGATCGCCAACTTCATCCCCTTCTC TGATCACAACCAGAGTCCTCGGAACATGTACCAGTGCCAGATGGGTAAGCAGACCATGGGCTTCCCGCTG CTCACCTACCAAGACCGATCAGATAATAAACTCTATCGTCTCCAGACACCCCAGAGCCCTCTAGTGAGAC CGTGCATGTATGATCATTATGACATGGACAACTATCCCATCGGGACAAACGCCATTGTGGCTGTGATCTC CTACACTGGCTATGATATGGAGGACGCCATGATTGTAAACAAGGCCTCCTGGGAACGAGGCTTTGCTCAT GGAAGTGTCTACAAGTCTGAGTTCATAGACCTCTCTGAGAAATTTAAGCAAGGGGATGATAGTCTGGTAT TTGGGGTCAAACCTGGTGACCCACGGGTTATGCAGAAGCTGGACAATGATGGCTTGCCATTCATAGGAGC AAAGCTGGAGTTTGGTGATCCTTACTACGGCTACCTAAACCTTAACACCGGAGAAGGCTTCGTGGTTTAC TATAAGAGTAAAGAAAACTGTGTTGTGGACAACATCAAAGTGTGCAGTAATGACACAGGAAGTGGGAAGT TCAAGTGCGTCTGCGTCACCGTCCGAGTCCCCCGGAACCCAACTATTGGAGATAAGTTTGCCAGCCGTCA CGGACAGAAGGGCATTTTGAGCAGATTGTGGCCAGCTGAGGACATGCCTTTCACAGAGAGTGGGATGATG CCGGACATTCTGTTTAATCCTCATGGGTTTCCCTCCCGTATGACCATAGGTATGTTAATCGAGAGTATGG CTGGGAAGTCAGCAGCTTTGCATGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCCGAGGAGAACTC TGCCCTAGAGTACTTTGGTGAGATGTTAAAGGCTGCCGGCTACAACTTCTATGGCACGGAGAGATTGTAC AGCGGCATCAGCGGGATGGAGCTGGAGGCTGACATTTTCATTGGTGTGGTTTATTACCAGCGCCTACGAC ACATGGTGTCAGACAAATTTCAAGTCAGAACAACTGGAGCCAGGGACAAAGTCACCAACCAGCCCATTGG AGGCAGGAACGTCCAGGGTGGGATCCGATTTGGGGAGATGGAGCGGGATGCTCTGTTGGCGCACGGCACA TCTTTCCTTCTGCATGACCGCCTCTTCAACTGCTCCGACCGCTCTGTGGCCCACGTATGCGTGAAGTGTG GCAGTTTGCTTTCTCCGCTGCTCGAGAAGCCTCCCCCATCTTGGTCTGCGATGCGTAACAGAAAATACAA CTGCACCGTCTGCGGCCGCAGTGACTCCATCGACACTGTCTCTGTGCCGTATGTTTTCCGGTACTTTGTA GCTGAGCTGGCTGCCATGAACATCAAAGTGAAACTGGACGTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202655 representing NM_031773
Red=Cloning site Green=Tags(s) MDVDSRWRNLPSGPSLKHLTDPSYAVPPEQQKAALQDLTRAHVDSFNYAVLEGLSHAVQAIPPFEFAFKD ERISLTIVDAAISPPAVPKGTICKELNIYPAECRGRRSTYRGKLTADISWAVNGVPKGIIKQFLGXVPIM VKSKLCNLYNLPPQVLIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKSRGLGYTQFGV SIHCVREEHSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDSFFKN SVSQMLRIVMEEGCHTQKQVLDYLGERFRVKLSLPDWYPNAEAAEFLFNQCICIHLKSNTDKFYLLCLMT RKLFALARGECMEDNPDSLVNQEVLTPGQLFLMFLKEKMENWLLSIKIALDKRAQKTNVSINNENLMKIF SMGTELTRPFEYLLATGNLRSKTGLGFMQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRKLL PESWGFLCPVHTPDGAPCGLLNHLTAVCEVVTKFVYTASIPALLCGLGVTPVDAAPCRPYSDCYPVLLDG VMVGWVDKELAPEVADTLRRFKVLRERRCSSLDGGGPDSHDRKAKPVPRAVPLHHSLQAGEACAEPGAGQ RRARWNYGAALHEHCHLRGRGFWWSFHTPGALPSQPAEVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPL LTYQDRSDNKLYRLQTPQSPLVRPCMYDHYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAH GSVYKSEFIDLSEKFKQGDDSLVFGVKPGDPRVMQKLDNDGLPFIGAKLEFGDPYYGYLNLNTGEGFVVY YKSKENCVVDNIKVCSNDTGSGKFKCVCVTVRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMM PDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLY SGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPIGGRNVQGGIRFGEMERDALLAHGT SFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTVCGRSDSIDTVSVPYVFRYFV AELAAMNIKVKLDVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031773 |
| ORF Size | 3405 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031773.1, NP_113961.1 |
| RefSeq Size | 3769 bp |
| RefSeq ORF | 3408 bp |
| Locus ID | 83582 |
| UniProt ID | O54888 |
| MW | 127.7 kDa |
| Gene Summary | mouse homolog is the second largest subunit of RNA polymerase I [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202655 | Polr1b (untagged ORF) - Rat polymerase (RNA) I polypeptide B (Polr1b), (10 ug) |
CNY 18,340.00 |
|
| RR202655L3 | Lenti ORF clone of Polr1b (Myc-DDK-tagged ORF) - Rat polymerase (RNA) I polypeptide B (Polr1b), (10 ug) |
CNY 19,760.00 |
|
| RR202655L4 | Lenti ORF clone of Polr1b (mGFP-tagged ORF) - Rat polymerase (RNA) I polypeptide B (Polr1b), (10 ug) |
CNY 21,760.00 |
