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Chrna7 (NM_012832) Rat Tagged ORF Clone
CAT＃: RR202164
- TrueORF®
Chrna7 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 7 (Chrna7), (10 ug)
"NM_012832" in other vectors (3)
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CNY 6,460.00
Product images
CNY 300.00
CNY 11,000.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BTX; nAChRa7; NARAD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202164 representing NM_012832
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCGGCGGGCGGGGAGGCATCTGGCTGGCTCTGGCCGCGGCGCTGCTGCACGTGTCCCTGCAAGGCG AGTTCCAGAGGAGGCTGTACAAGGAGCTGGTCAAGAACTACAACCCGCTGGAGAGGCCGGTGGCCAACGA CTCGCAGCCGCTCACCGTGTACTTCTCCCTGAGTCTCCTGCAGATCATGGATGTGGATGAGAAGAACCAA GTTTTAACCACCAACATTTGGCTACAAATGTCTTGGACAGATCACTATTTGCAGTGGAACATGTCTGAGT ACCCCGGAGTGAAGAATGTTCGTTTTCCAGATGGCCAGATTTGGAAACCAGACATTCTCCTCTATAACAG TGCTGATGAGCGCTTTGATGCCACGTTCCACACCAATGTTTTGGTGAATGCATCTGGGCATTGCCAGTAT CTCCCTCCAGGCATATTCAAGAGCTCCTGCTACATTGACGTTCGCTGGTTCCCTTTTGATGTGCAGCAGT GCAAACTGAAGTTTGGGTCCTGGTCCTATGGAGGGTGGTCACTGGACCTGCAAATGCAAGAGGCAGATAT CAGCAGCTATATCCCCAACGGAGAATGGGATCTCATGGGAATCCCTGGCAAAAGGAATGAGAAGTTCTAT GAGTGCTGCAAAGAGCCATACCCAGATGTCACCTACACAGTAACCATGCGCCGTAGGACACTCTACTATG GCCTCAATCTGCTCATCCCTTGTGTACTCATTTCAGCCCTGGCTCTGCTGGTATTCTTGCTGCCTGCAGA CTCTGGAGAGAAAATCTCTCTTGGAATAACTGTCTTACTTTCTCTGACTGTCTTCATGCTGCTTGTGGCT GAGATCATGCCAGCAACATCTGATTCTGTGCCCTTGATAGCACAATACTTCGCCAGCACCATGATCATCG TGGGCCTCTCTGTAGTGGTGACAGTGATTGTGCTGAGATATCACCACCATGACCCTGATGGTGGCAAAAT GCCTAAGTGGACCAGAATCATTCTCCTGAACTGGTGTGCATGGTTTCTGCGCATGAAGAGGCCCGGAGAG GACAAGGTGCGGCCAGCTTGTCAGCACAAGCCTCGGCGCTGCAGCCTGGCCAGTGTGGAGCTGAGTGCAG GTGCTGGGCCACCCACCAGCAATGGCAACCTGCTCTACATTGGCTTCCGAGGCCTGGAGGGCATGCACTG TGCCCCAACTCCAGACTCTGGGGTCGTATGTGGCCGTTTGGCCTGCTCCCCAACACATGATGAGCACCTC ATGCACGGTGCACACCCCTCTGATGGGGACCCCGACCTGGCCAAGATCCTGGAGGAGGTCCGCTACATCG CCAACCGCTTCCGCTGCCAGGACGAGAGTGAGGTGATCTGCAGTGAATGGAAGTTTGCAGCCTGCGTGGT GGACCGCTTGTGCCTCATGGCCTTTTCGGTCTTTACCATCATCTGTACCATCGGCATCCTCATGTCAGCT CCAAACTTTGTGGAGGCTGTGTCCAAAGACTTTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202164 representing NM_012832
Red=Cloning site Green=Tags(s) MCGGRGGIWLALAAALLHVSLQGEFQRRLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQ VLTTNIWLQMSWTDHYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNASGHCQY LPPGIFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQMQEADISSYIPNGEWDLMGIPGKRNEKFY ECCKEPYPDVTYTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVA EIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLRYHHHDPDGGKMPKWTRIILLNWCAWFLRMKRPGE DKVRPACQHKPRRCSLASVELSAGAGPPTSNGNLLYIGFRGLEGMHCAPTPDSGVVCGRLACSPTHDEHL MHGAHPSDGDPDLAKILEEVRYIANRFRCQDESEVICSEWKFAACVVDRLCLMAFSVFTIICTIGILMSA PNFVEAVSKDFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012832 |
| ORF Size | 1506 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012832.3, NP_036964.3 |
| RefSeq Size | 1509 bp |
| RefSeq ORF | 1509 bp |
| Locus ID | 25302 |
| UniProt ID | Q05941 |
| MW | 56.5 kDa |
| Gene Summary | subunit of nicotinic acetylcholline receptor; may mediate nicotine induced increase in tyrosine hydroxylase expression; may play a role in neuroprotection [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202164 | Chrna7 (untagged ORF) - Rat cholinergic receptor, nicotinic, alpha 7 (Chrna7), (10 ug) |
CNY 6,460.00 |
|
| RR202164L3 | Lenti ORF clone of Chrna7 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 7 (Chrna7), (10 ug) |
CNY 8,360.00 |
|
| RR202164L4 | Lenti ORF clone of Chrna7 (mGFP-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 7 (Chrna7), (10 ug) |
CNY 9,120.00 |
