Lrp4 (NM_031322) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Megf7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201769 representing NM_031322 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGTGGTGGGGCGCGCTGCTGCTCGGCGCCCTGCTCTGCGCACACGGCACAGCCAGCAATCTGG AGTGTGCGTGTGGTCGGAGCCACTTCACATGTGCAGTGAGCGCGCTCGGCGAATGTACCTGCATCCCCGC CCAGTGGCAGTGTGATGGTGACAATGACTGTGGAGACCACAGCGATGAGGATGGCTGTACGCTGCCTACC TGTTCCCCTCTGGACTTCCACTGTGACAACGGCAAGTGCATCCGACGCTCCTGGGTGTGTGATGGGGACA ATGACTGTGAAGATGACTCCGATGAGCAGGACTGTCCCCCCCGGGAGTGTGAGGAGGACGAGTTCCCCTG CCAGAACGGCTACTGCATCCGGAGTCTGTGGCACTGTGATGGGGACAATGACTGTGGTGACAACAGTGAC GAACAGTGCGACATGCGCAAGTGCTCAGACAAGGAATTCCGCTGCAGTGATGGGAGCTGCATTGCTGAGC ATTGGTACTGTGATGGAGACACAGACTGCAAAGACGGCTCTGATGAGGAAAGCTGCCCCTCAGCAGTACC CTCTCCTCCTTGCAACCTGGAAGAGTTCCAGTGTGCCTACGGCCGCCGCATCCTTGACATCTACCACTGT GACGGGGATGATGACTGCGGAGACTGGTCAGACGAGTCCGACTGCTCCTCCCACCAGCCCTGCCGCTCTG GGGAATTCATGTGTGACAGTGGCCTGTGCGTCAACGCAGGCTGGCGCTGTGATGGTGATGCGGACTGCGA TGACCAGTCTGATGAACGTAACTGCACCACCTCCATGTGCACAGCTGAACAGTTTCGCTGTCGGTCAGGC CGCTGTGTCCGTCTGTCCTGGCGCTGTGATGGGGAGGATGACTGCGCGGACAACAGTGATGAAGAAAACT GTGAGAACACAGGAAGCCCCCAATGCGCCTCTGACCAGTTCCTGTGTTGGAATGGGCGTTGTATTGGGCA GAGGAAGCTCTGCAATGGGGTCAATGACTGCGGCGACAACAGTGACGAAAGCCCACAGCAGAACTGTCGG CCCCGGACGGGTGAGGAGAACTGCAATGTTAACAACGGTGGCTGTGCCCAGAAGTGTCAGATGATTCGAG GGGCAGTGCAGTGTACCTGCCACACAGGCTACCGGCTCACAGAGGATGGGAGAACGTGCCAGGATGTGAA TGAATGTGCTGAGGAGGGATACTGCAGCCAGGGCTGCACAAACAGCGAAGGGGCTTTCCAGTGCTGGTGC GAAGCCGGTTATGAGCTGCGGCCCGATCGGCGCAGCTGCAAGGCTCTGGGGCCGGAACCTGTGCTACTGT TTGCCAATCGCATCGACATCCGGCAGGTGCTCCCGCACCGCTCCGAATACACGCTGCTACTGAACAACCT GGAGAACGCCATTGCCCTTGACTTTCATCATCGGCGGGAGCTGGTCTTCTGGTCTGATGTCACCCTGGAC CGCATCCTCCGTGCCAACCTTAATGGCAGCAATGTGGAGGAGGTGGTGTCTACTGGGCTAGAGAGCCCAG GCGGCTTGGCTGTGGATTGGGTCCATGACAAGCTCTACTGGACTGACTCCGGGACGTCGAGGATTGAGGT GGCTAATCTGGACGGGGCCCACCGGAAGGTGCTGCTGTGGCAGAGCCTGGAGAAGCCCAGGGCCATTGCC CTGCACCCCATGGAGGGAACCATCTATTGGACAGACTGGGGCAACACCCCCCGCATCGAGGCCTCCAGCA TGGACGGCTCTGGACGTCGCATCATTGCTGACACTCATCTTTTCTGGCCCAACGGCCTCACCATCGACTA CGCTGGACGCCGTATGTACTGGGTGGACGCTAAGCACCACGTCATCGAGAGAGCCAATCTAGATGGGAGT CACCGCAAGGCTGTCATTAGCCAGGGCCTCCCACATCCCTTTGCCATCACAGTGTTTGAAGACAGCTTGT ATTGGACAGACTGGCACACCAAGAGCATCAATAGTGCCAACAAGTTCACTGGGAAGAACCAGGAGATCAT TCGCAACAAGCTCCACTTTCCTATGGACATTCATACCTTGCACCCCCAGCGCCAGCCTGCAGGGAAAAAC CGCTGCGGGGACAACAACGGAGGTTGCACTCACCTGTGTCTACCCAGTGGCCAGAACTACACTTGTGCCT GCCCCACCGGCTTCCGAAAAATCAACAGCCACGCCTGTGCCCAGAGTCTTGACAAGTTCCTGCTTTTTGC CCGAAGGATGGACATCCGTCGGATCAGCTTCGACACAGAGGACCTGTCCGACGATGTCATCCCACTGGCT GACGTGCGCAGTGCTGTGGCCCTTGACTGGGACTCCCGGGATGACCACGTGTACTGGACAGATGTCAGCA CTGATACCATCAGCAGGGCCAAGTGGGATGGAACAGGACAGAAGGTGGTAGTTGACACCAGTTTGGAGAG CCCTGCTGGCCTGGCAATTGATTGGGTCACCAACAAACTCTACTGGACAGATGCAGGTACAGACCGGATT GAAGTGGCCAACACGGACGGCAGCATGAGGACAGTGCTCATCTGGGAGAACCTTGATCGTCCTCGGGACA TCGTGGTAGAACCCATGGGCGGGTACATGTATTGGACTGACTGGGGTGCAAGTCCCAAGATTGAACGAGC TGGCATGGATGCCTCCAACCGACAAGTCATCATCTCTTCGAATCTGACATGGCCTAATGGATTAGCTATT GACTATGGGTCCCAGCGGCTGTACTGGGCTGATGCCGGCATGAAGACTATTGAATTTGCTGGGCTGGATG GCAGCAAGAGGAAGGTACTAATTGGAAGTCAACTCCCCCACCCTTTCGGGCTGACTCTATACGGACAGCG CATCTATTGGACTGACTGGCAGACCAAAAGTATCCAAAGTGCTGACCGGCTGACTGGGCTAGACCGGGAG ACTCTGCAGGAGAACCTGGAGAATCTTATGGACATCCATGTCTTCCACCGCCAGAGGCCTCCAGTGACGA CACCATGTGCTGTGGAGAATGGCGGCTGCAGCCATTTGTGTCTTCGGTCCCCAAGTCCGAGCGGCTTCAG CTGTACCTGCCCCACAGGTATCAACCTGCTGCTTGATGGCAAGACATGCTCACCAGGCATGAACAGTTTC CTCATCTTCGCCAGGAGGATTGATGTTCGCATGGTCTCCTTGGACATCCCTTATTTTGCTGATGTGGTGG TACCCATCAACATGACCATGAAGAACACCATTGCCATTGGAGTGGACCCGCTGGAAGGAAAAGTGTACTG GTCTGACAGCACACTGCACAGGATCAGCCGTGCCAGCCTGGATGGCTCACAGCACGAGGACATCATCACT ACAGGTTTGCAGACCACAGATGGACTTGCAGTGGATGCCATCGGCCGGAAAGTGTATTGGACAGACACGG GAACGAATCGGATTGAAGTTGGCAACCTGGATGGGTCTATGCGGAAAGTGTTGGTGTGGCAGAACCTTGA CAGTCCCCGGGCCATTGTGCTATACCATGAAATGGGGTTCATGTACTGGACAGACTGGGGGGAGAATGCC AAACTAGAGCGTTCCGGGATGGATGGCTCAGACCGTACTGTGCTCATCAACAACAACCTAGGGTGGCCTA ACGGACTGACTGTGGACAAGACCAGCTCCCAACTGCTGTGGGCTGATGCCCATACTGAGAGAATTGAGGT GGCTGACCTGAACGGTGCCAATCGGCATACATTGGTGTCACCAGTGCAGCACCCATATGGCCTCACTCTG CTTGACTCTTACATCTACTGGACTGACTGGCAGACCCGTAGCATCCACCGGGCCGACAAGAGTACTGGCA GCAATGTTATCCTCGTGAGGTCCAACCTGCCAGGCCTCATGGACATCCAAGCTGTTGACCGGGCACAGCC ACTGGGTTTTAATAAGTGTGGTTCGAGAAATGGCGGCTGCTCCCACCTCTGTTTACCTCGGCCCTCTGGT TTCTCCTGTGCCTGCCCCACTGGCATTCAGCTGAAGGGGGATGGGAAGACCTGTGACCCCTCTCCGGAGA CCTACCTGCTCTTCTCTAGCCGGGGCTCCATTCGCCGTATCTCACTGGACACCGATGACCACACAGACGT GCATGTCCCTGTTCCTGGGCTCAACAATGTCATCTCCCTAGACTATGACAGTGTGGATGGAAAGGTCTAT TACACAGACGTGTTCCTGGATGTTATCAGGCGAGCAGACCTGAATGGCAGCAACATGGAGACTGTTATTG GGCATGGGCTGAAGACCACTGATGGGCTGGCAGTGGACTGGGTTGCCAGAAACCTGTACTGGACAGATAC AGGCCGAAATACCATCGAAGCATCTCGGCTAGATGGTTCCTGTCGCAAAGTGCTAATCAACAACAGTCTG GACGAGCCCCGGGCCATTGCTGTTTTCCCCAGAAAGGGGTACCTCTTCTGGACAGACTGGGGCCACATTG CCAAGATTGAACGGGCAAACCTGGATGGTTCTGAGAGGAAGGTCCTCATCAATGCAGACCTAGGCTGGCC CAATGGCCTTACCTTGGACTATGATACCCGCAGGATCTACTGGGTAGATGCACATCTGGATCGGATTGAG AGTGCTGACCTCAATGGGAAGCTGAGGCAGGTCTTGGTCAGCCATGTGTCCCACCCCTTTGCCCTCACAC AGCAGGACAGGTGGATCTACTGGACAGACTGGCAGACCAAATCCATCCAGCGTGTCGACAAGTACTCAGG CCGCAACAAGGAGACAGTGCTGGCCAATGTGGAGGGACTCATGGATATCATCGTGGTGTCCCCTCAACGG CAGACAGGGACCAATGCCTGTGGTGTGAACAATGGTGGCTGTAGCCACCTCTGCTTTGCCAGAGCCTCTG ACTTTGTGTGCGCCTGTCCTGATGAGCCAGACAGTCATCCCTGCTCCCTCGTGCCTGGCCTCATGCCTCC TGCTCCCAGAGCTACCAGCCTGAATGAAAAGAGCCCAGTGCTCCCCAACACATTGCCCACCACCTTACAT TCTTCTACCACCAGGACCCGCACATCTCCAGAGGGGGCAGAAGGAAGATGCTCTGAAAGGGACGCCCAGC TGGGCCTCTGTGCACATTCCAATGAAGCTGTACCTGCTGCTCCAGGTGAAGGACTGCATGTCAGCTACGC CGTTGGTGGACTCCTCAGCGTCCTGCTGATTTTGTTGGTGACTGCAGCTTTGATGCTATACAGACACAGA AAATCCAAATTCACGGATCCTGGAATGGGAAACCTGACCTACAGCAACCCCTCCTACCGAACTTCCACTC AGGAAGTGAAAATTGAAGCAGCTCCCAAACCAGCCATGTATAATCAGCTGTGCTATAAGAAAGAGGGTGG ACCTGACCACAGCTACACCAAGGAGAAGATCAAGATTGTAGAGGGAATTCACCTCCTGGCTGGGCATGAT GCTGAATGGGGTGACCTCAAGCAACTGCGCAGCTCCAGGGGGGGCCTTCTACGGGATCACGTATGCATGA AGACAGACACGGTGTCCATCCAGGCCAGCTCTGGCTCCCTAGATGACACGGAGACAGAGCAGCTGCTGCA GGAAGAGCAGTCTGAGTGTAGCAGTGTTCACACTGCAACCACTCCAGAGAGACGGGGCTCTCTGCCAGAC ACAGGCTGGAAACATGAACGCAAGCTCTCCTCAGAAAGCCAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201769 representing NM_031322 Red=Cloning site Green=Tags(s) MRRWWGALLLGALLCAHGTASNLECACGRSHFTCAVSALGECTCIPAQWQCDGDNDCGDHSDEDGCTLPT CSPLDFHCDNGKCIRRSWVCDGDNDCEDDSDEQDCPPRECEEDEFPCQNGYCIRSLWHCDGDNDCGDNSD EQCDMRKCSDKEFRCSDGSCIAEHWYCDGDTDCKDGSDEESCPSAVPSPPCNLEEFQCAYGRRILDIYHC DGDDDCGDWSDESDCSSHQPCRSGEFMCDSGLCVNAGWRCDGDADCDDQSDERNCTTSMCTAEQFRCRSG RCVRLSWRCDGEDDCADNSDEENCENTGSPQCASDQFLCWNGRCIGQRKLCNGVNDCGDNSDESPQQNCR PRTGEENCNVNNGGCAQKCQMIRGAVQCTCHTGYRLTEDGRTCQDVNECAEEGYCSQGCTNSEGAFQCWC EAGYELRPDRRSCKALGPEPVLLFANRIDIRQVLPHRSEYTLLLNNLENAIALDFHHRRELVFWSDVTLD RILRANLNGSNVEEVVSTGLESPGGLAVDWVHDKLYWTDSGTSRIEVANLDGAHRKVLLWQSLEKPRAIA LHPMEGTIYWTDWGNTPRIEASSMDGSGRRIIADTHLFWPNGLTIDYAGRRMYWVDAKHHVIERANLDGS HRKAVISQGLPHPFAITVFEDSLYWTDWHTKSINSANKFTGKNQEIIRNKLHFPMDIHTLHPQRQPAGKN RCGDNNGGCTHLCLPSGQNYTCACPTGFRKINSHACAQSLDKFLLFARRMDIRRISFDTEDLSDDVIPLA DVRSAVALDWDSRDDHVYWTDVSTDTISRAKWDGTGQKVVVDTSLESPAGLAIDWVTNKLYWTDAGTDRI EVANTDGSMRTVLIWENLDRPRDIVVEPMGGYMYWTDWGASPKIERAGMDASNRQVIISSNLTWPNGLAI DYGSQRLYWADAGMKTIEFAGLDGSKRKVLIGSQLPHPFGLTLYGQRIYWTDWQTKSIQSADRLTGLDRE TLQENLENLMDIHVFHRQRPPVTTPCAVENGGCSHLCLRSPSPSGFSCTCPTGINLLLDGKTCSPGMNSF LIFARRIDVRMVSLDIPYFADVVVPINMTMKNTIAIGVDPLEGKVYWSDSTLHRISRASLDGSQHEDIIT TGLQTTDGLAVDAIGRKVYWTDTGTNRIEVGNLDGSMRKVLVWQNLDSPRAIVLYHEMGFMYWTDWGENA KLERSGMDGSDRTVLINNNLGWPNGLTVDKTSSQLLWADAHTERIEVADLNGANRHTLVSPVQHPYGLTL LDSYIYWTDWQTRSIHRADKSTGSNVILVRSNLPGLMDIQAVDRAQPLGFNKCGSRNGGCSHLCLPRPSG FSCACPTGIQLKGDGKTCDPSPETYLLFSSRGSIRRISLDTDDHTDVHVPVPGLNNVISLDYDSVDGKVY YTDVFLDVIRRADLNGSNMETVIGHGLKTTDGLAVDWVARNLYWTDTGRNTIEASRLDGSCRKVLINNSL DEPRAIAVFPRKGYLFWTDWGHIAKIERANLDGSERKVLINADLGWPNGLTLDYDTRRIYWVDAHLDRIE SADLNGKLRQVLVSHVSHPFALTQQDRWIYWTDWQTKSIQRVDKYSGRNKETVLANVEGLMDIIVVSPQR QTGTNACGVNNGGCSHLCFARASDFVCACPDEPDSHPCSLVPGLMPPAPRATSLNEKSPVLPNTLPTTLH SSTTRTRTSPEGAEGRCSERDAQLGLCAHSNEAVPAAPGEGLHVSYAVGGLLSVLLILLVTAALMLYRHR KSKFTDPGMGNLTYSNPSYRTSTQEVKIEAAPKPAMYNQLCYKKEGGPDHSYTKEKIKIVEGIHLLAGHD AEWGDLKQLRSSRGGLLRDHVCMKTDTVSIQASSGSLDDTETEQLLQEEQSECSSVHTATTPERRGSLPD TGWKHERKLSSESQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031322
ORF Size	5715 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031322.3, NP_112612.2
RefSeq Size	7784 bp
RefSeq ORF	5718 bp
Locus ID	83469
MW	211.9 kDa
Gene Summary	mouse homolog has similarity to the low density lipoprotein receptor family, however, it does not bind very low density lipoprotein [RGD, Feb 2006]

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