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Grik3 (NM_181373) Rat Tagged ORF Clone
CAT＃: RR200120
- TrueORF®
Grik3 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 3 (Grik3), transcript variant 2, (10 ug)
"NM_181373" in other vectors (3)
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CNY 11,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GluK3; gluR-7; GluR7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200120 representing NM_181373
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGCTCCCTGGCGGCGCCTCCGGAGTCTGGTTTGGGAATACTGGGCCGGGTTCCTCGTGTGCGCCT TCTGGATCCCAGACTCGCGCGGGATGCCCCACGTCATCCGGATCGGCGGAATCTTTGAGTACGCGGACGG CCCCAACGCCCAGGTCATGAACGCTGAGGAGCACGCCTTTCGGTTTTCTGCCAATATCATCAACAGGAAC AGAACTCTGCTGCCCAACACGACCCTGACTTACGACATTCAGAGGATTCACTTCCATGACAGTTTTGAGG CCACCAAGAAGGCCTGTGACCAGTTGGCGCTCGGTGTGGTAGCCATCTTTGGGCCATCCCAGGGCTCCTG CACCAATGCCGTCCAGTCCATCTGCAATGCCTTGGAGGTTCCTCACATCCAACTGCGCTGGAAGCACCAC CCCCTGGACAACAAGGACACCTTCTACGTGAACCTCTACCCCGACTACGCCTCTCTCAGCCACGCCATCC TCGACTTGGTCCAGTCCCTCAAGTGGCGGTCAGCCACCGTAGTCTATGATGACAGTACAGGTCTCATCCG GCTGCAGGAGCTCATCATGGCTCCATCTAGGTACAACATCCGCCTGAAGATTCGCCAGCTCCCCATCGAC TCCGATGACTCACGCCCCTTGCTCAAAGAGATGAAGCGGGGCCGGGAGTTCCGTATCATCTTTGACTGCA GTCACACCATGGCAGCCCAGATCCTCAAGCAGGCCATGGCCATGGGCATGATGACGGAATACTACCACTT CATCTTCACCACTCTGGATCTCTATGCGCTAGACCTGGAACCCTACCGCTACTCGGGAGTGAACCTGACT GGGTTCCGCATACTCAACGTGGACAACCCCCATGTCTCAGCCATTGTGGAGAAGTGGTCCATGGAGCGGC TACAGGCAGCTCCCCGGGCAGAGTCAGGCCTGCTGGATGGAGTGATGATGACCGATGCAGCCCTGCTCTA CGATGCGGTCCACATTGTGTCTGTGTGCTACCAGCGAGCGCCGCAGATGACTGTGAACTCCCTACAGTGC CATCGGCACAAGGCCTGGCGCTTCGGTGGCCGCTTCATGAACTTCATCAAGGAGGCTCAATGGGAAGGAT TAACTGGACGGATTGTTTTCAACAAAACTAGTGGCTTGCGGACTGATTTTGATCTGGACATCATCAGCCT CAAGGAAGATGGCCTCGAGAAGGTCGGGGTGTGGAGTCCAGCTGACGGTCTCAATATCACTGAGGTTGCC AAAGGCCGAGGTCCTAATGTCACCGACTCTCTGACCAACAGGTCACTCATCGTCACCACTCTGCTGGAGG AGCCTTTTGTCATGTTCCGCAAGTCTGATAGGACCCTTTACGGCAATGACCGGTTCGAGGGCTACTGCAT CGACTTGCTCAAGGAGCTGGCGCACATCCTGGGCTTCTCCTACGAGATCCGGCTGGTGGAAGACGGCAAG TACGGGGCACAGGACGACAAGGGCCAGTGGAACGGCATGGTCAAGGAACTCATTGACCACAAAGCAGATC TGGCTGTGGCTCCCCTGACCATCACCCATGTCCGAGAGAAGGCCATTGACTTCTCTAAGCCTTTTATGAC CCTCGGAGTGAGCATCTTATATCGAAAACCCAATGGCACCAACCCCAGTGTCTTCTCCTTCCTCAACCCC CTGTCCCCAGACATCTGGATGTACGTGCTACTCGCCTACCTGGGTGTCAGCTGTGTCCTCTTCGTCATTG CCAGATTCAGCCCTTATGAATGGTATGATGCCCACCCCTGCAACCCCGGCTCTGAGGTGGTGGAGAATAA CTTCACGCTGCTCAACAGCTTCTGGTTTGGAATGGGCTCCCTGATGCAACAAGGATCTGAACTGATGCCC AAAGCTCTGTCTACCCGCATCATTGGCGGCATCTGGTGGTTCTTCACCCTTATTATCATCTCCTCCTACA CGGCCAACCTGGCTGCCTTCCTGACCGTGGAGCGCATGGAGTCACCCATCGACTCTGCCGATGACCTGGC CAAGCAGACCAAAATAGAGTACGGTGCTGTCAAGGATGGGGCCACCATGACCTTCTTCAAGAAATCCAAG ATCTCCACCTTTGAGAAGATGTGGGCCTTCATGAGCAGCAAGCCCTCGGCTCTGGTGAAGAACAATGAGG AGGGCATCCAGCGGACACTCACAGCTGACTACGCTCTGCTCATGGAGTCCACGACCATAGAGTACATCAC ACAAAGGAACTGCAATCTCACCCAGATCGGCGGCCTCATCGATTCCAAGGGCTACGGCATCGGCACGCCC ATGGGCTCCCCCTACAGGGACAAAATCACCATCGCCATTCTGCAGCTGCAGGAGGAGGACAAGCTGCACA TCATGAAGGAGAAGTGGTGGCGAGGCAGCGGGTGCCCCGAGGAGGAGAACAAGGAGGCCAGCGCACTGGG CATCCAGAAGATTGGCGGCATCTTCATCGTCCTGGCTGCCGGCTTAGTCCTGTCCGTGTTGGTGGCAGTG GGCGAGTTTATATACAAACTCCGCAAGACAGCGGAACGGGAGCAGGTGAGGCCCTGGAGGAGGCTGCGTT GGACAGGGAAGGAAGCGCTCTTTCTGCAGCACAGTGGCCGACGAGATCCGCTTCTCCCTCACCTGCCAGC GGCGTCTCAAGCACAAGCCACAGCCTCCTATGATGGTCAAGACAGATGCGGTTATCAACATGCACACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200120 representing NM_181373
Red=Cloning site Green=Tags(s) MTAPWRRLRSLVWEYWAGFLVCAFWIPDSRGMPHVIRIGGIFEYADGPNAQVMNAEEHAFRFSANIINRN RTLLPNTTLTYDIQRIHFHDSFEATKKACDQLALGVVAIFGPSQGSCTNAVQSICNALEVPHIQLRWKHH PLDNKDTFYVNLYPDYASLSHAILDLVQSLKWRSATVVYDDSTGLIRLQELIMAPSRYNIRLKIRQLPID SDDSRPLLKEMKRGREFRIIFDCSHTMAAQILKQAMAMGMMTEYYHFIFTTLDLYALDLEPYRYSGVNLT GFRILNVDNPHVSAIVEKWSMERLQAAPRAESGLLDGVMMTDAALLYDAVHIVSVCYQRAPQMTVNSLQC HRHKAWRFGGRFMNFIKEAQWEGLTGRIVFNKTSGLRTDFDLDIISLKEDGLEKVGVWSPADGLNITEVA KGRGPNVTDSLTNRSLIVTTLLEEPFVMFRKSDRTLYGNDRFEGYCIDLLKELAHILGFSYEIRLVEDGK YGAQDDKGQWNGMVKELIDHKADLAVAPLTITHVREKAIDFSKPFMTLGVSILYRKPNGTNPSVFSFLNP LSPDIWMYVLLAYLGVSCVLFVIARFSPYEWYDAHPCNPGSEVVENNFTLLNSFWFGMGSLMQQGSELMP KALSTRIIGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVKDGATMTFFKKSK ISTFEKMWAFMSSKPSALVKNNEEGIQRTLTADYALLMESTTIEYITQRNCNLTQIGGLIDSKGYGIGTP MGSPYRDKITIAILQLQEEDKLHIMKEKWWRGSGCPEEENKEASALGIQKIGGIFIVLAAGLVLSVLVAV GEFIYKLRKTAEREQVRPWRRLRWTGKEALFLQHSGRRDPLLPHLPAASQAQATASYDGQDRCGYQHAHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181373 |
| ORF Size | 2730 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181373.3, NP_852038.2 |
| RefSeq Size | 3634 bp |
| RefSeq ORF | 2733 bp |
| Locus ID | 298521 |
| UniProt ID | P42264 |
| MW | 103.1 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Compared to other kainate or AMPA receptors, this subunit exhibits a lower sensitivity to glutamate, and thus may play a unique role in neurotransmission in the brain. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200120 | Grik3 (untagged ORF) - Rat glutamate receptor, ionotropic, kainate 3 (Grik3), transcript variant 2, (10 ug) |
CNY 11,780.00 |
|
| RR200120L3 | Lenti ORF clone of Grik3 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 3 (Grik3), transcript variant 2, (10 ug) |
CNY 13,400.00 |
|
| RR200120L4 | Lenti ORF clone of Grik3 (mGFP-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 3 (Grik3), transcript variant 2, (10 ug) |
CNY 14,820.00 |
