Pax3 (NM_053710) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216768 representing NM_053710 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACGCTGGCCGGCGCTGTGCCCAGGATGATGCGGCCCGGCCCGGGGCAGAATTACCCACGCAGCG GCTTCCCACTGGAAGTGTCTACACCCCTCGGCCAGGGCCGAGTCAATCAGCTCGGAGGAGTATTTATCAA CGGCAGGCCTCTGCCCAACCACATCCGCCACAAGATCGTGGAGATGGCCCACCACGGCATTCGGCCTTGC GTCATCTCTCGCCAACTTCGCGTGTCCCACGGCTGTGTCTCTAAGATCTTATGCAGGTACCAGGAGACAG GCTCCATCCGACCTGGCGCCATTGGCGGCAGCAAGCCCAAGCAGGTGACAACGCCTGACGTGGAGAAGAA AATTGAGGAATACAAAAGAGAGAACCCAGGCATGTTTAGCTGGGAAATCAGAGACAAATTACTCAAGGAC GCTGTCTGTGATCGGAACACTGTGCCCTCAGTGAGTTCCATCAGTCGCATCCTGAGGAGTAAATTTGGAA AAGGAGAAGAGGAGGAGGCGGATTTAGAAAGGAAAGAAGCCGAGGAAAGCGAGAAAAAGGCTAAACACAG CATCGATGGCATCCTAAGTGAGCGAGCCTCCGCACCTCAATCAGATGAAGGCTCGGACATTGACTCAGAA CCTGATTTACCGCTGAAGAGGAAGCAGCGCAGGAGTAGAACCACCTTCACCGCAGAGCAGCTGGAGGAAC TGGAGCGCGCTTTCGAGAGAACCCACTATCCAGACATTTACACCAGGGAGGAGCTGGCCCAGAGGGCGAA GCTTACTGAGGCCCGAGTGCAGGTCTGGTTTAGCAACCGCCGTGCAAGATGGAGGAAGCAAGCTGGAGCC AATCAACTGATGGCTTTCAACCATCTCATTCCGGGGGGATTCCCTCCCACCGCCATGCCGACCCTGCCAA CATACCAGCTGTCGGAGACCTCTTACCAGCCCACGTCTATTCCACAAGCCGTATCAGATCCCAGTAGTAC TGTTCACAGACCTCAGCCGCTTCCTCCGAGCACTGTCCACCAAAGCACCATTCCTTCGAATGCAGACAGC AGCTCTGCCTACTGCCTTCCCAGCACCAGGCATGGATTTTCAAGCTACACCGACAGCTTCGTGCCTCCAT CGGGGCCCTCCAACCCCATGAACCCCGCCATTGGCAATGGCCTTTCACCTCAGGTAATGGGACTTCTGAC CAACCACGGTGGGGTACCGCACCAGCCTCAGACCGACTATGCTCTCTCCCCTCTCACTGGGGGCCTGGAA CCCACGACCACGGTGTCAGCCAGCTGCAGTCAGAGATTGGAGCACATGAAGAATGTGGACAGTCTGCCCA CATCTCAGCCCTACTGTCCCCCCACCTATAGTACCACAGGCTACAGCATGGACCCTGTCACGGGCTACCA GTATGGGCAGTATGGACAAAGTGCCTTTCATTATCTCAAGCCAGATATTGCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053710
Insert Size	1455 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053710.1, NP_446162.1
RefSeq Size	2197 bp
RefSeq ORF	1455 bp
Locus ID	114502
Gene Summary	may act as a transcription factor and play a role in Schwann cell development; may be regulated by transcriptional modulator Sox10 [RGD, Feb 2006]

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