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SUN2 (NM_001199579) Human Tagged ORF Clone
CAT＃: RG234801
- TrueORF®
SUN2 (tGFP-tagged) - Homo sapiens Sad1 and UNC84 domain containing 2 (SUN2), transcript variant 1
ORF Plasmid: DDK
"NM_001199579" in other vectors (2)
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CNY 6,370.00
货期*
3周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | UNC84B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234801 representing NM_001199579
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGAAGAAGCCAGCGCCTCACGCGCTACTCCCAGGGTGACGATGACGGCAGCAGCAGCAGCGGAG GGAGCTCGGTGGCTGGGAGTCAGAGCACCCTGTTTAAAGACAGTCCTCTCAGGACCTTGAAGAGGAAATC CAGCAACATGAAGCGCCTGTCCCCAGCGCCACAGCTGGGCCCGTCCTCTGATGCACACACCTCCTACTAC AGTGAGTCGCTGGTCCACGAGTCCTGGTTCCCACCCAGGAGCTCCCTGGAGGAACTGCATGGTGACGCCA ACTGGGGTGAGGACCTGCGGGTGCGGAGGAGGAGAGGCACGGGTGGCTCAGAGAGCAGCAGGGCCAGCGG GCTTGTGGGGCGCAAGGCCACCGAGGACTTCCTGGGCTCTTCCTCGGGCTACTCCTCTGAGGACGACTAC GTGGAAGACAGTGAAGGAAGAGGCTCGAAAGTGACAGAGACTGAGCCAGTGTCCTCGTTCCCCGCAGGCT ACTCGGATGTGGACCAGCAGAGTTCCAGCTCGCGGCTCCGAAGCGCCGTCTCACGGGCGGGCTCCTTACT CTGGATGGTGGCCACTTCGCCAGGCCGGCTCTTCAGACTTCTCTACTGGTGGGCTGGCACCACCTGGTAC CGCCTGACCACAGCTGCCTCCCTCCTTGACGTCTTCGTTTTAACCAGGCGCTTCTCGTCCCTGAAGACGT TCCTCTGGTTCCTGCTGCCGCTGCTCTTGCTGACGTGCCTGACGTATGGTGCTTGGTATTTCTACCCCTA TGGGCTGCAGACATTCCACCCTGCTTTGGTTTCCTGGTGGGCAGCGAAGGACAGCAGGAGGCCGGATGAG GGCTGGGAAGCCAGAGACTCATCGCCACATTTCCAGGCTGAGCAGCGTGTTATGTCCCGGGTACACTCTC TGGAGCGGCGTCTGGAAGCTCTTGCTGCTGAATTTTCCTCCAACTGGCAGAAGGAGGCCATGCGGCTGGA ACGTCTGGAGCTGCGGCAAGGGGCTCCTGGCCAGGGAGGTGGTGGTGGCCTGAGCCACGAGGACACCCTG GCGCTGCTGGAGGGGCTAGTGAGCCGCCGTGAAGCTGCCCTGAAGGAGGATTTCCGCAGGGAAACTGCTG CTCGCATCCAGGAAGAACTGTCTGCCCTGAGAGCAGAGCATCAGCAAGACTCAGAAGACCTCTTCAAGAA GATCGTCCGGGCCTCCCAGGAGTCCGAGGCTCGCATCCAGCAGCTGAAGTCAGAGTGGCAAAGCATGACC CAGGAGTCCTTCCAGGAGAGCTCTGTGAAGGAGCTGAGGCGGCTGGAGGACCAGCTGGCCGGCCTGCAGC AGGAGCTGGCGGCTCTGGCACTGAAGCAGAGCTCGGTGGCGGAAGAAGTGGGCCTGCTGCCCCAGCAGAT CCAGGCCGTGCGGGACGACGTGGAATCTCAGTTCCCGGCCTGGATCAGTCAGTTCCTTGCCCGAGGTGGA GGGGGCCGCGTGGGGCTCCTTCAGAGAGAGGAGATGCAAGCTCAGCTGCGAGAGCTGGAGAGCAAGATCC TCACCCATGTGGCAGAGATGCAGGGCAAGTCGGCCAGGGAAGCCGCGGCCTCCCTGAGCCTGACGCTGCA GAAAGAAGGTGTGATTGGAGTGACAGAGGAGCAGGTGCACCACATCGTGAAGCAGGCCCTGCAGCGCTAC AGTGAGGACCGCATCGGGCTGGCAGACTACGCCCTGGAGTCAGGAGGGGCCAGCGTCATCAGCACCCGAT GTTCTGAGACCTACGAGACCAAGACGGCCCTCCTCAGCCTCTTCGGCATCCCCCTGTGGTACCACTCCCA GTCACCCCGAGTCATCCTCCAGCCAGATGTGCACCCAGGCAACTGCTGGGCCTTCCAGGGGCCACAAGGC TTCGCCGTGGTCCGCCTCTCTGCCCGCATCCGCCCCACAGCCGTTACCTTAGAGCATGTGCCCAAGGCCT TGTCACCCAACAGCACTATCTCCAGTGCCCCCAAGGACTTCGCCATCTTTGGGTTTGACGAAGACCTGCA GCAGGAGGGGACACTCCTTGGCAAGTTCACTTACGATCAGGACGGCGAGCCTATTCAGACGTTTCACTTT CAGGCCCCTACGATGGCCACGTACCAGGTGGTGGAGCTGCGGATCCTGACTAACTGGGGCCACCCCGAGT ACACCTGCATCTACCGCTTCAGAGTGCATGGGGAGCCCGCCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234801 representing NM_001199579
Red=Cloning site Green=Tags(s) MSRRSQRLTRYSQGDDDGSSSSGGSSVAGSQSTLFKDSPLRTLKRKSSNMKRLSPAPQLGPSSDAHTSYY SESLVHESWFPPRSSLEELHGDANWGEDLRVRRRRGTGGSESSRASGLVGRKATEDFLGSSSGYSSEDDY VEDSEGRGSKVTETEPVSSFPAGYSDVDQQSSSSRLRSAVSRAGSLLWMVATSPGRLFRLLYWWAGTTWY RLTTAASLLDVFVLTRRFSSLKTFLWFLLPLLLLTCLTYGAWYFYPYGLQTFHPALVSWWAAKDSRRPDE GWEARDSSPHFQAEQRVMSRVHSLERRLEALAAEFSSNWQKEAMRLERLELRQGAPGQGGGGGLSHEDTL ALLEGLVSRREAALKEDFRRETAARIQEELSALRAEHQQDSEDLFKKIVRASQESEARIQQLKSEWQSMT QESFQESSVKELRRLEDQLAGLQQELAALALKQSSVAEEVGLLPQQIQAVRDDVESQFPAWISQFLARGG GGRVGLLQREEMQAQLRELESKILTHVAEMQGKSAREAAASLSLTLQKEGVIGVTEEQVHHIVKQALQRY SEDRIGLADYALESGGASVISTRCSETYETKTALLSLFGIPLWYHSQSPRVILQPDVHPGNCWAFQGPQG FAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQEGTLLGKFTYDQDGEPIQTFHF QAPTMATYQVVELRILTNWGHPEYTCIYRFRVHGEPAH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199579 |
| ORF Size | 2214 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199579.2 |
| RefSeq Size | 4070 bp |
| RefSeq ORF | 2217 bp |
| Locus ID | 25777 |
| UniProt ID | Q9UH99 |
| Protein Families | Transmembrane |
| Gene Summary | SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM, Nov 2010] |
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