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CNOT10 (NM_001256741) Human Tagged ORF Clone
CAT＃: RG234770
- TrueORF®
CNOT10 (tGFP-tagged) - Homo sapiens CCR4-NOT transcription complex, subunit 10 (CNOT10), transcript variant 2
ORF Plasmid: DDK
"NM_001256741" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234770 representing NM_001256741
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGACAAGCCTGCAGATCAGGGAGCAGAGAAACATGAAGGCACAGGTCAGTCCTCTGGGATCA CTGATCAAGAGAAGGAGTTATCCACCAATGCTTTCCAAGCTTTCACATCTGGAAATTATGATGCCTGTCT ACAACACCTTGCCTGTCTACAAGATATAAACAAAGATGATTATAAAATAATTTTGAATACAGCAGTAGCT GAGTTTTTTAAAAGTAACCAAACAACAACAGATAATTTGAGACAAACACTTAACCAGCTGAAGAATCAGG TCCACTCAGCTGTTGAAGAAATGGATGGATTAGATGATGTTGAAAACAGCATGTTGTACTATAATCAAGC AGTCATTCTTTATCATCTGCGGCAGTATACAGAAGCCATATCAGTTGGTGAAAAACTTTATCAGTTCATA GAGCCTTTTGAAGAAAAATTTGCCCAAGCAGTGTGTTTTTTGCTTGTAGACCTGTATATATTAACCTACC AAGCTGAGAAAGCTTTGCATCTTCTTGCTGTCCTAGAAAAAATGATTTCACAGGGTAACAATAACAAAAA TGGAAAGAATGAGACTGGTAATAACAACAACAAAGATGGATCTAATCATAAAGCTGAAAGTGGAGCTCTA ATAGAAGCTGCAAAATCAAAGATACATCAGTACAAAGTACGAGCTTATATCCAAATGAAGTCTCTGAAAG CATGTAAAAGGGAAATCAAGTCAGTCATGAATACAGCTGGAAATTCCGCACCCTCTCTCTTTCTTAAAAG CAATTTTGAGTACTTAAGAGGTAATTATCGAAAAGCCGTGAAGCTATTAAATAGTTCAAACATTGCTGAG CATCCAGGATTCATGAAAACAGGTGAATGCTTGAGATGCATGTTCTGGAATAACCTTGGTTGCATCCATT TTGCCATGAGCAAGCACAATTTGGGAATATTCTACTTTAAAAAGGCTCTGCAAGAGAATGACAATGTCTG TGCACAGCTCAGTGCAGGTAGCACTGATCCAGGTAAAAAATTTTCAGGAAGACCCATGTGTACGTTACTA ACCAATAAGAGATATGAGTTGCTGTATAACTGTGGAATTCAGCTTCTTCACATTGGAAGGCCTCTTGCTG CCTTCGAATGTCTGATTGAAGCTGTTCAGGTTTATCATGCAAATCCTCGCCTCTGGCTACGGCTGGCTGA ATGCTGCATTGCTGCCAATAAGGGGACTTCTGAACAAGAAACTAAAGGCCTTCCCAGCAAAAAAGGAATT GTACAGTCTATTGTTGGTCAAGGCTATCATCGTAAAATAGTTTTGGCATCACAGTCTATACAGAATACTG TTTATAATGATGGGCAGTCTTCGGCCATTCCTGTAGCCAGTATGGAGTTTGCAGCCATATGTCTCAGAAA TGCCTTGTTGCTGCTACCTGAAGAACAGCAAGATCCAAAGCAGGAAAATGGGGCTAAAAATAGTAATCAA TTAGGTGGGAACACAGAGAGCAGCGAAAGCAGTGAAACTTGCAGGTGCTCCATACTTGCTTGCAGTGCCT ACGTGGCTCTGGCTTTGGGTGATAACCTCATGGCTTTGAATCATGCAGATAAACTTCTTCAGCAGCCCAA GCTGTCAGGATCTCTTAAGTTTTTGGGACATTTATATGCTGCAGAAGCCCTCATCTCTCTCGACAGAATA TCTGATGCCATTACTCACTTGAACCCGGAGAATGTCACTGATGTCTCCTTAGGGATCTCTTCAAATGAGC AGGACCAAGGATCAGACAAAGGTGAAAATGAAGCAATGGAATCCTCTGGTAAGCGGGCCCCTCAGTGCTA CCCCAGTTCCGTCAACTCTGCCAGGACTGTGATGCTGTTCAACCTTGGCAGCGCTTACTGCCTGAGGAGC GAATATGACAAAGCCCGAAAGTGTCTCCACCAGGCGGCTTCAATGATCCATCCTAAAGAGGTGCCCCCTG AGGCCATCTTGCTGGCAGTCTACCTTGAACTGCAGAATGGTAATACTCAGCTGGCCTTACAGATCATCAA AAGGAATCAGCTGCTCCCTGCAGTGAAAACACACTCTGAAGTGAGAAAGAAGCCAGTGTTTCAGCCTGTC CACCCGATCCAGCCCATCCAAATGCCGGCTTTCACCACTGTGCAGAGAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234770 representing NM_001256741
Red=Cloning site Green=Tags(s) MAADKPADQGAEKHEGTGQSSGITDQEKELSTNAFQAFTSGNYDACLQHLACLQDINKDDYKIILNTAVA EFFKSNQTTTDNLRQTLNQLKNQVHSAVEEMDGLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFI EPFEEKFAQAVCFLLVDLYILTYQAEKALHLLAVLEKMISQGNNNKNGKNETGNNNNKDGSNHKAESGAL IEAAKSKIHQYKVRAYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSSNIAE HPGFMKTGECLRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGSTDPGKKFSGRPMCTLL TNKRYELLYNCGIQLLHIGRPLAAFECLIEAVQVYHANPRLWLRLAECCIAANKGTSEQETKGLPSKKGI VQSIVGQGYHRKIVLASQSIQNTVYNDGQSSAIPVASMEFAAICLRNALLLLPEEQQDPKQENGAKNSNQ LGGNTESSESSETCRCSILACSAYVALALGDNLMALNHADKLLQQPKLSGSLKFLGHLYAAEALISLDRI SDAITHLNPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRAPQCYPSSVNSARTVMLFNLGSAYCLRS EYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLALQIIKRNQLLPAVKTHSEVRKKPVFQPV HPIQPIQMPAFTTVQRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256741 |
| ORF Size | 2151 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256741.2 |
| RefSeq Size | 2748 bp |
| RefSeq ORF | 2154 bp |
| Locus ID | 25904 |
| UniProt ID | Q9H9A5 |
| Protein Pathways | RNA degradation |
| Gene Summary | Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.[UniProtKB/Swiss-Prot Function] |
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