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ARHGAP21 (NM_020824) Human Tagged ORF Clone
CAT＃: RG229458
- TrueORF®
ARHGAP21 (tGFP-tagged) - Human Rho GTPase activating protein 21 (ARHGAP21)
ORF Plasmid: DDK
"NM_020824" in other vectors (3)
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CNY 20,900.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARHGAP10 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229458 representing NM_020824
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCCACGCGTCGGACTGGTCTGTCTGAGGGAGATGGTGACAAGCTCAAGGCCTGCGAGGTCTCAA AAAATAAAGATGGAAAAGAACAAAGTGAAACTGTATCACTGTCTGAAGATGAAACATTCTCCTGGCCAGG TCCCAAAACAGTTACGTTGAAAAGAACATCTCAAGGCTTTGGTTTTACATTAAGACATTTTATTGTTTAT CCCCCAGAGTCTGCAATTCAATTTTCATATAAGGATGAAGAAAATGGAAACAGAGGAGGAAAACAAAGAA ACCGCTTGGAACCAATGGATACCATATTTGTTAAGCAAGTTAAAGAAGGAGGACCTGCTTTTGAAGCTGG ATTATGTACAGGTGACCGAATTATAAAAGTCAATGGAGAAAGTGTTATTGGCAAAACCTATTCCCAAGTA ATTGCTTTAATTCAAAACAGTGATACAACATTGGAACTTAGTGTTATGCCAAAAGATGAAGACATTCTCC AAGTGCTACAGTTTACAAAGGATGTCACAGCACTGGCATATTCTCAAGATGCCTACCTGAAAGGCAACGA AGCTTATAGCGGCAATGCCCGCAATATACCTGAACCTCCACCAATCTGCTATCCCTGGCTGCCATCTGCC CCATCAGCCATGGCACAGCCAGTTGAAATATCTCCTCCTGACTCATCATTGAGCAAACAGCAAACCAGTA CACCAGTACTGACACAACCTGGTAGGGCCTATAGAATGGAAATACAAGTGCCTCCATCACCAACAGATGT TGCAAAATCAAACACAGCAGTGTGTGTTTGCAATGAAAGTGTAAGGACTGTCATTGTGCCTTCTGAGAAG GTTGTAGATTTGTTATCCAATAGAAACAACCATACAGGTCCTTCACATAGAACTGAAGAAGTGAGGTATG GCGTGAGTGAGCAGACCTCTTTAAAAACAGTGTCAAGAACCACATCACCACCATTATCAATTCCCACCAC TCATCTAATTCATCAGCCTGCAGGCTCCAGATCACTGGAACCTTCTGGAATTTTACTTAAGTCTGGAAAT TACAGTGGACATTCTGATGGAATCTCAAGCAGCAGATCTCAAGCTGTGGAGGCTCCCTCTGTATCTGTTA ATCACTATTCGCCAAATTCCCATCAGCACATAGACTGGAAAAACTATAAAACTTACAAAGAGTATATTGA TAACAGACGATTGCACATAGGTTGTCGGACAATACAAGAAAGATTAGATAGTTTAAGAGCAGCATCTCAA AGCACGACAGATTATAACCAGGTCGTCCCCAACCGCACTACTTTGCAGGGACGACGTCGAAGCACCTCTC ATGATCGAGTGCCCCAGTCTGTCCAGATACGGCAACGCAGTGTGTCCCAAGAAAGACTGGAAGATTCTGT GCTAATGAAGTATTGTCCAAGAAGTGCATCTCAAGGAGCACTGACGTCTCCATCTGTTAGTTTTAGTAAT CATAGAACTCGTTCATGGGATTATATTGAGGGACAGGATGAAACCTTAGAAAATGTCAATTCTGGAACTC CAATACCTGATTCCAATGGAGAGAAAAAACAGACTTACAAGTGGAGTGGGTTTACTGAACAGGATGATAG ACGAGGTATTTGTGAAAGACCTAGGCAGCAAGAAATTCATAAATCTTTTCGAGGTTCCAATTTTACTGTG GCTCCAAGCGTTGTTAATTCTGATAACAGGCGAATGAGTGGTAGAGGAGTGGGATCTGTGTCGCAGTTTA AAAAAATTCCACCAGATCTAAAAACATTGCAGTCAAACAGAAATTTTCAGACTACTTGTGGAATGTCACT GCCTCGGGGTATTTCACAAGACAGGTCACCTCTTGTGAAAGTCCGAAGTAATTCTCTGAAAGCTCCTTCC ACGCATGTCACAAAACCATCATTTAGCCAGAAATCATTTGTTTCTATCAAAGACCAAAGACCAGTAAATC ACTTGCATCAGAACAGTCTGTTGAATCAGCAGACATGGGTAAGGACTGACAGTGCCCCCGATCAGCAAGT GGAGACTGGGAAATCCCCCTCTTTATCTGGAGCCTCTGCCAAGCCTGCCCCTCAGTCGAGTGAAAACGCT GGTACTTCAGATTTAGAACTACCTGTCAGTCAAAGGAATCAAGATTTAAGTTTACAAGAGGCTGAAACTG AGCAATCAGATACTTTAGATAATAAAGAAGCTGTCATCCTAAGGGAAAAACCTCCATCTGGACGCCAGAC ACCGCAGCCTTTAAGGCATCAGTCTTACATCTTGGCAGTAAATGACCAGGAGACCGGGTCAGACACTACC TGCTGGCTGCCCAATGATGCACGTCGAGAGGTCCACATAAAAAGAATGGAGGAAAGAAAAGCCTCGAGTA CCAGTCCGCCTGGCGATTCTTTGGCTTCCATCCCATTTATAGATGAACCAACTAGCCCTAGCATTGATCA TGATATTGCACATATCCCTGCCTCTGCTGTTATATCAGCCTCTACCTCTCAGGTCCCCTCCATAGCAACA GTTCCTCCTTGCCTCACAACTTCAGCTCCATTAATTCGCCGTCAGCTCTCACATGACCACGAATCTGTTG GCCCTCCTAGCCTGGATGCTCAGCCCAACTCAAAGACAGAAAGATCAAAATCATATGATGAGGGTCTGGA TGATTACAGAGAAGATGCAAAATTGTCCTTTAAGCACGTATCTAGTCTGAAGGGAATCAAGATCGCAGAC AGCCAAAAGTCATCAGAAGACTCTGGGTCCAGAAAAGATTCTTCCTCAGAGGTCTTCAGTGATGCTGCCA AGGAAGGGTGGCTTCATTTCCGACCCCTTGTCACCGATAAGGGCAAGCGAGTTGGTGGAAGTATTCGGCC ATGGAAACAGATGTATGTTGTCCTTCGGGGTCATTCACTTTACCTGTACAAAGATAAAAGAGAGCAGACG ACTCCGTCTGAGGAAGAGCAGCCCATCAGTGTTAATGCTTGCTTGATAGACATCTCTTACAGTGAGACCA AGAGGAAAAATGTGTTTCGACTCACCACGTCCGACTGTGAATGCCTGTTTCAGGCTGAAGACAGAGATGA TATGCTAGCTTGGATCAAGACGATCCAGGAGAGCAGCAACCTAAACGAAGAGGACACTGGAGTCACTAAC AGGGATCTAATTAGTCGAAGAATAAAAGAATACAACAATCTGATGAGCAAAGCAGAACAGTTGCCAAAAA CACCTCGCCAGAGTCTCAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCC ACACTCTCCGAAGGAAGAGTCGGAAAGGAAACTTCTCAGTAAAGATGATACCAGTCCCCCAAAAGACAAA GGCACATGGAGAAAAGGCATTCCAAGTATCATGAGAAAGACATTTGAGAAAAAGCCAACTGCTACAGGAA CTTTCGGCGTCCGACTAGATGACTGCCCACCAGCTCATACTAATCGGTATATTCCATTAATAGTTGACAT ATGTTGCAAATTAGTTGAAGAAAGAGGTCTTGAATATACAGGTATTTATAGAGTTCCTGGAAATAATGCA GCCATCTCAAGTATGCAAGAAGAACTCAACAAGGGAATGGCTGATATTGATATACAAGATGATAAATGGC GAGATTTGAATGTGATAAGCAGTTTACTAAAATCCTTCTTCAGAAAACTCCCTGAGCCTCTCTTCACAAA TGATAAATATGCTGATTTTATTGAAGCCAATCGTAAAGAAGATCCTCTAGATCGTCTGAAAACATTAAAA AGACTAATTCACGATTTGCCTGAACATCATTATGAAACACTTAAGTTCCTTTCAGCTCATCTGAAGACAG TGGCAGAAAATTCAGAAAAAAATAAGATGGAACCAAGAAACCTAGCAATAGTGTTTGGTCCCACCCTTGT TCGAACATCAGAAGACAACATGACCCACATGGTCACCCACATGCCTGACCAGTACAAGATTGTAGAAACG CTCATCCAGCACCATGACTGGTTTTTCACAGAAGAAGGTGCTGAAGAGCCTCTTACAACAGTGCAGGAGG AAAGCACAGTAGACTCCCAGCCAGTGCCAAACATAGATCATTTACTCACCAACATTGGAAGGACAGGAGT CTCCCCAGGAGATGTATCAGATTCAGCTACTAGTGACTCAACAAAATCTAAGGGTTCTTGGGGATCTGGA AAGGATCAGTATAGCAGGGAACTGCTTGTGTCCTCCATCTTTGCAGCTGCTAGTCGCAAGAGGAAGAAGC CGAAAGAAAAAGCACAGCCTAGCAGCTCAGAAGATGAACTGGACAATGTATTTTTTAAGAAAGAAAATGT GGAACAGTGTCACAATGATACTAAAGAGGAGTCCAAAAAAGAAAGTGAGACACTGGGCAGAAAACAGAAG ATCATCATTGCCAAAGAAAACAGCACTAGGAAAGACCCCAGCACGACAAAAGATGAAAAGATATCACTAG GAAAAGAGAGCACGCCTTCTGAAGAACCCTCACCACCACACAACTCAAAACACAACAAGTCACCAACTCT CAGCTGTCGCTTTGCCATCCTGAAAGAGAGCCCCAGGTCACTTCTGGCACAGAAGTCCTCCCACCTTGAA GAGACAGGCTCTGACTCTGGCACTTTGCTCAGCACGTCTTCCCAGGCCTCCCTGGCAAGGTTTTCCATGA AGAAATCAACCAGTCCAGAAACGAAACATAGCGAGTTTTTGGCCAACGTCAGCACCATCACCTCAGATTA TTCCACCACATCGTCTGCTACATACTTGACTAGCCTGGACTCCAGTCGACTGAGCCCTGAGGTGCAATCC GTGGCAGAGAGCAAGGGGGACGAGGCAGATGACGAGAGAAGCGAACTCATCAGTGAAGGGCGGCCTGTGG AAACCGACAGCGAGAGCGAGTTTCCCGTGTTCCCCACAGCCTTGACTTCAGAGAGGCTTTTCCGAGGAAA ACTGCAAGAAGTGACTAAGAGCAGCCGGAGAAATTCTGAAGGAAGTGAATTAAGTTGCACCGAGGGAAGT TTAACATCAAGTTTAGATAGCCGGAGACAGCTCTTCAGTTCCCATAAACTCATCGAATGTGATACTCTTT CCAGGAAAAAATCAGCTAGATTCAAGTCAGATAGTGGAAGTCTAGGAGATGCCAAGAATGAGAAAGAAGC ACCTTCGTTAACTAAAGTGTTTGATGTTATGAAAAAAGGAAAGTCAACTGGGAGTTTACTGACACCCACC AGAGGCGAATCCGAAAAACAGGAACCCACATGGAAAACGAAAATAGCAGATCGGTTAAAACTGAGACCCA GAGCCCCTGCGGATGACATGTTTGGAGTAGGGAATCACAAAGTGAATGCCGAGACTGCTAAAAGGAAAAG CATCCGGCGCAGACATACACTAGGAGGGCACAGAGATGCTACCGAAATCAGCGTTTTGAATTTTTGGAAA GTGCATGAGCAGAGCGGGGAGAGAGAATCTGAACTTTCAGCTGTAAACCGGTTAAAACCAAAATGCTCAG CCCAGGACCTTTCCATCTCAGACTGGCTGGCCAGGGAACGCCTACGCACCAGTACCTCTGACCTTAGCAG AGGAGAAATCGGAGATCCCCAGACAGAGAACCCAAGCACACGAGAAATAGCCACGACCGACACACCTTTG TCTCTTCATTGCAACACAGGCAGTTCTTCCAGCACCTTGGCTTCAACAAACAGGCCCCTTCTTTCCATAC CACCACAGTCACCTGACCAAATAAACGGAGAAAGCTTCCAGAACGTGAGCAAAAATGCTAGTTCTGCAGC GAATGCCCAACCTCATAAACTGTCTGAAACCCCAGGCAGTAAAGCAGAGTTTCATCCCTGTCTT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229458 representing NM_020824
Red=Cloning site Green=Tags(s) MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVY PPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQV IALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSA PSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEK VVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGN YSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQ STTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSN HRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTV APSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPS THVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENA GTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTT CWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIAT VPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIAD SQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQT TPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTN RDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDK GTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNA AISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLK RLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVET LIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSG KDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQK IIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLE ETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQS VAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGS LTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPT RGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWK VHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPL SLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020824 |
| ORF Size | 5874 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020824.4 |
| RefSeq Size | 7185 bp |
| RefSeq ORF | 5877 bp |
| Locus ID | 57584 |
| UniProt ID | Q5T5U3 |
| Domains | RhoGAP, PDZ, PH |
| Gene Summary | ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3 complex (MIM 604221) and F-actin dynamics at the Golgi through control of CDC42 activity (Dubois et al., 2005 [PubMed 15793564]).[supplied by OMIM, Mar 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229458 | ARHGAP21 (Myc-DDK-tagged)-Human Rho GTPase activating protein 21 (ARHGAP21) |
CNY 18,990.00 |
|
| SC309106 | ARHGAP21 (untagged)-Human Rho GTPase activating protein 21 (ARHGAP21) |
CNY 37,810.00 |
|
| SC328093 | ARHGAP21 (untagged)-Human Rho GTPase activating protein 21 (ARHGAP21) |
CNY 37,810.00 |
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