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PEG3 (NM_001146187) Human Tagged ORF Clone
CAT＃: RG229078
- TrueORF®
PEG3 (tGFP-tagged) - Human paternally expressed 3 (PEG3), transcript variant 5
ORF Plasmid: DDK
"NM_001146187" in other vectors (2)
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CNY 16,060.00
货期*
详询
规格
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经常一起买 (5)
PEG3 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PW1; ZKSCAN22; ZNF904; ZSCAN24 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229078 representing NM_001146187
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGCGACGACGACATGACCCGGAACAGAAGAG AGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGGTGACCGGGACTGGGACCGGAGGGGCAGAAGCAG AGACATGGAGCCACGAGACCGCTGGTCCCACACCAGGAACCCAAGAAGCAGGATGCCTCCGCGGGATCTT TCCCTTCCTGTGGTGGCGAAAACAAGCTTTGAAATGGACAGAGAGGACGACAGGGACTCCAGGGCTTATG AGTCCCGATCTCAGGATGCTGAATCATACCAAAATGTGGTGGACCTCGCTGAGGACAGGAAACCTCACAA CACAATCCAGGACAACATGGAAAACTACAGGAAGCTGCTCTCCCTCGGAGTGCAGCTTGCTGAAGACGAT GGCCACTCCCACATGACGCAGGGCCACTCATCAAGATCCAAGAGAAGTGCCTACCCAAGCACCAGTCGAG GTCTAAAAACTATGCCTGAAGCCAAAAAATCAACCCACCGGCGGGGGATTTGTGAAGATGAATCTTCCCA CGGAGTGATAATGGAAAAATTCATCAAGGATGTGTCACGCAGTTCCAAATCGGGAAGAGCAAGGGAGTCA AGCGACCGGTCACAGAGATTCCCCAGAATGTCAGATGATAACTGGAAGGACATTTCATTGAACAAGAGGG AGTCAGTGATCCAGCAGCGGGTTTATGAAGGGAATGCATTTAGGGGAGGCTTTAGGTTTAATTCAACCCT TGTTTCCAGAAAGAGAGTTCTTGAAAGAAAGAGGCGCTATCATTTTGACACAGATGGGAAGGGCTCGATT CACGATCAAAAAGGCTGTCCCAGGAAGAAGCCCTTTGAATGTGGTAGTGAGATGAGAAAAGCCATGAGCG TGAGCAGCCTGAGCAGCCTCAGCTCCCCCTCCTTTACCGAGTCACAGCCAATTGATTTTGGGGCAATGCC ATATGTATGTGATGAGTGTGGGAGGTCGTTCAGTGTCATCTCAGAATTTGTTGAGCACCAGATCATGCAT ACTAGAGAGAACCTCTATGAGTATGGTGAGTCCTTTATCCACAGTGTGGCTGTCAGTGAAGTTCAGAAAA GTCAGGTTGGAGGGAAACGTTTTGAATGTAAGGACTGTGGAGAGACCTTCAATAAGAGTGCCGCCTTGGC TGAACATCGGAAGATTCATGCTAGAGGTTATCTTGTGGAATGTAAGAATCAGGAATGTGAGGAAGCCTTC ATGCCTAGCCCCACCTTTAGTGAGCTTCAGAAAATATATGGCAAAGACAAATTCTACGAGTGCAGGGTGT GTAAGGAAACCTTCCTTCATAGTTCTGCCCTGATTGAGCACCAGAAAATCCACTTTGGGGATGACAAAGA TAATGAGCGTGAACATGAACGTGAACGTGAACGTGAGCGCGGGGAAACCTTTAGGCCCAGCCCAGCCCTT AATGAGTTTCAGAAAATGTATGGTAAAGAGAAAATGTACGAATGTAAGGTGTGTGGGGAGACTTTCCTTC ATAGCTCATCCCTGAAAGAACATCAGAAAATCCATACTAGAGGGAACCCATTTGAAAACAAGGGTAAAGT GTGTGAGGAAACCTTTATTCCTGGTCAGTCCCTTAAAAGGCGTCAGAAAACTTACAATAAGGAGAAGCTC TGTGACTTTACAGATGGCCGGGATGCCTTCATGCAAAGCTCAGAGCTCAGTGAGCATCAGAAAATTCATT CTCGAAAGAACCTCTTTGAAGGCAGAGGGTATGAGAAATCTGTCATTCATAGTGGGCCATTCACTGAATC TCAGAAGAGTCATACTATAACAAGACCTCTTGAAAGTGATGAGGACGAAAAGGCGTTCACCATTAGCTCT AACCCCTATGAAAACCAGAAGATTCCCACTAAGGAAAATGTCTACGAGGCAAAATCATATGAGAGGTCTG TTATTCATAGCTTAGCCTCTGTGGAAGCTCAGAAAAGTCACAGTGTAGCAGGGCCCAGTAAACCAAAAGT AATGGCAGAGTCTACCATTCAGAGCTTCGATGCTATCAACCATCAGAGAGTTCGTGCTGGAGGGAACACC TCTGAAGGAAGGGAATACAGTAGGTCTGTTATCCATAGCTTAGTGGCTTCCAAACCTCCAAGAAGTCACA ATGGAAATGAATTGGTGGAATCTAATGAGAAGGGAGAATCCTCCATTTATATCTCAGACCTTAATGATAA GCGACAGAAGATTCCTGCCAGAGAGAACCCTTGTGAAGGGGGCAGTAAGAATCGCAACTATGAAGACTCT GTCATACAGAGTGTATTCCGTGCCAAACCTCAGAAAAGTGTTCCTGGAGAGGGATCTGGTGAGTTTAAGA AGGATGGCGAATTCTCTGTTCCCAGCTCAAATGTCCGTGAATACCAGAAGGCTCGTGCTAAAAAGAAATA CATTGAGCATAGGAGCAATGAGACCTCTGTAATTCACTCTCTGCCTTTTGGTGAACAAACATTTCGCCCT CGAGGGATGCTCTATGAATGTCAGGAGTGTGGGGAGTGCTTTGCTCATAGCTCTGACCTCACTGAGCACC AGAAGATTCATGATAGGGAGAAGCCCTCTGGAAGCAGAAACTATGAATGGTCTGTCATTCGCAGCTTGGC CCCTACTGACCCTCAAACAAGTTACGCCCAAGAGCAGTATGCTAAAGAGCAAGCGCGGAACAAATGTAAG GACTTCAGACAATTTTTTGCTACCAGCGAAGACCTCAACACAAACCAGAAAATCTATGACCAAGAGAAGT CTCATGGCGAGGAGTCTCAAGGCGAGAATACTGATGGGGAGGAGACCCACAGCGAGGAGACCCATGGTCA GGAGACAATTGAAGACCCTGTCATTCAAGGCTCAGACATGGAAGACCCTCAGAAGGATGACCCTGATGAC AAAATCTATGAATGTGAGGACTGTGGCCTGGGCTTTGTGGATCTCACAGACCTCACAGACCATCAGAAAG TCCACAGCAGGAAGTGCCTGGTTGACAGTCGGGAGTACACACATTCTGTAATTCACACCCATTCCATCAG CGAGTATCAGAGAGATTACACTGGAGAGCAGCTGTATGAATGTCCAAAGTGTGGGGAATCTTTTATTCAT AGCTCATTCCTTTTCGAGCATCAGAGAATCCATGAACAAGACCAGTTGTATTCCATGAAGGGGTGTGATG ATGGTTTTATTGCCCTCTTGCCCATGAAGCCACGGAGGAATCGTGCTGCAGAGAGGAATCCTGCTCTTGC TGGGTCGGCCATTCGATGCCTTTTGTGTGGACAAGGCTTCATTCATAGCTCTGCCCTTAATGAGCATATG AGACTTCATAGGGAAGATGATTTACTGGAGCAGAGCCAGATGGCTGAGGAAGCTATCATTCCAGGCTTAG CCCTCACTGAGTTTCAGAGAAGTCAGACCGAAGAGAGACTCTTTGAATGTGCAGTCTGTGGAGAATCTTT CGTCAACCCAGCAGAACTTGCAGATCACGTAACTGTTCATAAGAATGAGCCCTATGAGTACGGGTCCTCC TATACTCACACCTCATTTCTTACTGAGCCCCTCAAAGGAGCTATACCATTCTATGAATGCAAGGATTGTG GTAAGTCCTTTATTCATAGCACAGTCCTCACTAAACATAAGGAGCTTCATCTGGAAGAAGAAGAAGAAGA TGAAGCAGCAGCAGCTGCAGCAGCAGCAGCCCAGGAAGTTGAAGCCAATGTCCATGTTCCACAAGTAGTT CTGAGGATTCAGGGCTTAAACGTAGAGGCTGCTGAGCCAGAAGTGGAGGCTGCCGAGCCAGAAGTGGAGG CTGCTGAGCCAGAAGTGGAGGCTGCTGAGCCAAACGGAGAGGCTGAAGGGCCAGATGGAGAGGCTGCAGA GCCCATTGGAGAGGCTGGACAGCCAAATGGAGAGGCCGAGCAGCCAAATGGGGATGCTGATGAGCCAGAT GGTGCAGGTATTGAAGACCCAGAAGAAAGAGCTGAAGAGCCAGAGGGAAAAGCTGAAGAGCCAGAGGGAG ATGCCGACGAGCCTGACGGTGTGGGAATTGAAGACCCAGAAGAAGGTGAAGATCAAGAGATTCAGGTAGA AGAACCATACTATGACTGCCATGAATGCACAGAAACCTTCACTTCCAGCACAGCATTCAGTGAACACCTG AAAACTCATGCCAGCATGATCATATTTGAGCCTGCAAATGCCTTTGGGGAGTGCTCAGGCTACATCGAAC GTGCCAGCACCAGCACAGGTGGTGCCAATCAAGCTGATGAGAAGTACTTCAAATGTGACGTCTGTGGGCA GCTCTTCAATGACCGCCTGTCCCTCGCCAGACACCAGAATACCCACACTGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229078 representing NM_001146187
Red=Cloning site Green=Tags(s) MYQPEDDNNSDVTSDDDMTRNRRESSPPHSVHSFSGDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDL SLPVVAKTSFEMDREDDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLGVQLAEDD GHSHMTQGHSSRSKRSAYPSTSRGLKTMPEAKKSTHRRGICEDESSHGVIMEKFIKDVSRSSKSGRARES SDRSQRFPRMSDDNWKDISLNKRESVIQQRVYEGNAFRGGFRFNSTLVSRKRVLERKRRYHFDTDGKGSI HDQKGCPRKKPFECGSEMRKAMSVSSLSSLSSPSFTESQPIDFGAMPYVCDECGRSFSVISEFVEHQIMH TRENLYEYGESFIHSVAVSEVQKSQVGGKRFECKDCGETFNKSAALAEHRKIHARGYLVECKNQECEEAF MPSPTFSELQKIYGKDKFYECRVCKETFLHSSALIEHQKIHFGDDKDNEREHERERERERGETFRPSPAL NEFQKMYGKEKMYECKVCGETFLHSSSLKEHQKIHTRGNPFENKGKVCEETFIPGQSLKRRQKTYNKEKL CDFTDGRDAFMQSSELSEHQKIHSRKNLFEGRGYEKSVIHSGPFTESQKSHTITRPLESDEDEKAFTISS NPYENQKIPTKENVYEAKSYERSVIHSLASVEAQKSHSVAGPSKPKVMAESTIQSFDAINHQRVRAGGNT SEGREYSRSVIHSLVASKPPRSHNGNELVESNEKGESSIYISDLNDKRQKIPARENPCEGGSKNRNYEDS VIQSVFRAKPQKSVPGEGSGEFKKDGEFSVPSSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRP RGMLYECQECGECFAHSSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCK DFRQFFATSEDLNTNQKIYDQEKSHGEESQGENTDGEETHSEETHGQETIEDPVIQGSDMEDPQKDDPDD KIYECEDCGLGFVDLTDLTDHQKVHSRKCLVDSREYTHSVIHTHSISEYQRDYTGEQLYECPKCGESFIH SSFLFEHQRIHEQDQLYSMKGCDDGFIALLPMKPRRNRAAERNPALAGSAIRCLLCGQGFIHSSALNEHM RLHREDDLLEQSQMAEEAIIPGLALTEFQRSQTEERLFECAVCGESFVNPAELADHVTVHKNEPYEYGSS YTHTSFLTEPLKGAIPFYECKDCGKSFIHSTVLTKHKELHLEEEEEDEAAAAAAAAAQEVEANVHVPQVV LRIQGLNVEAAEPEVEAAEPEVEAAEPEVEAAEPNGEAEGPDGEAAEPIGEAGQPNGEAEQPNGDADEPD GAGIEDPEERAEEPEGKAEEPEGDADEPDGVGIEDPEEGEDQEIQVEEPYYDCHECTETFTSSTAFSEHL KTHASMIIFEPANAFGECSGYIERASTSTGGANQADEKYFKCDVCGQLFNDRLSLARHQNTHTG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146187 |
| ORF Size | 4392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146187.1, NP_001139659.1 |
| RefSeq Size | 8381 bp |
| RefSeq ORF | 4395 bp |
| Locus ID | 5178 |
| UniProt ID | Q9GZU2 |
| Protein Families | Transcription Factors |
| Gene Summary | In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009] |
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