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ATP7A (NM_000052) Human Tagged ORF Clone
CAT＃: RG224789
- TrueORF®
ATP7A (tGFP-tagged) - Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_000052" in other vectors (3)
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CNY 16,992.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DSMAX; MK; MNK; SMAX3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224789 ORF sequence, codon optimized.
Due to the complexity of NM_000052, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAAGTATGGGTGTGAATTCTGTTACCATTTCTGTTGAGGGTATGACTTGCAATTCCTGTGTTT GGACCATTGAGCAGCAGATTGGAAAAGTGAATGGTGTGCATCACATTAAGGTATCACTGGAAGAAAAAAA TGCAACTATTATTTATGACCCTAAACTACAGACTCCAAAGACCCTACAGGAAGCTATTGATGACATGGGC TTTGATGCTGTTATCCATAATCCTGACCCTCTCCCTGTTTTAACTGACACCTTGTTTCTGACTGTTACGG CGTCACTGACTTTGCCATGGGACCATATCCAAAGCACATTGCTGAAGACCAAGGGTGTGACAGACATTAA AATTTACCCTCAGAAAAGAACTGTAGCAGTGACAATAATCCCTTCTATAGTGAATGCCAATCAGATAAAA GAGCTGGTTCCAGAACTCAGTTTAGATACTGGGACACTGGAGAAAAAGTCAGGAGCTTGTGAAGATCATA GTATGGCTCAAGCTGGTGAAGTCGTGCTGAAGATGAAAGTGGAAGGGATGACCTGCCATTCATGTACTAG CACTATTGAAGGAAAAATTGGGAAACTGCAAGGTGTTCAGCGAATTAAAGTCTCCCTGGACAATCAAGAA GCTACTATTGTTTATCAACCTCATCTTATCTCAGTAGAGGAAATGAAAAAGCAGATTGAAGCTATGGGCT TTCCAGCATTTGTCAAAAAGCAGCCCAAGTACCTCAAATTGGGAGCTATTGATGTAGAACGTCTAAAGAA CACACCAGTTAAATCCTCAGAAGGGTCACAGCAAAGGAGTCCATCATATACCAATGATTCAACAGCCACT TTCATCATTGATGGCATGCATTGTAAATCATGTGTGTCAAATATTGAAAGTACTTTATCTGCACTCCAAT ATGTAAGCAGCATAGTAGTTTCTTTAGAGAATAGGTCTGCCATTGTGAAGTATAATGCAAGCTCAGTCAC TCCAGAATCCCTGAGAAAAGCAATAGAGGCTGTATCACCGGGGCTATATAGAGTTAGTATCACAAGTGAA GTTGAGAGTACCTCAAACTCTCCCTCCAGCTCATCTCTTCAGAAGATTCCTTTGAATGTAGTTAGCCAGC CTCTGACACAAGAAACTGTGATAAACATTGATGGCATGACTTGTAATTCCTGTGTGCAGTCTATTGAGGG TGTCATATCAAAAAAGCCAGGTGTAAAATCCATACGAGTCTCCCTTGCAAATAGCAATGGGACTGTTGAG TATGATCCTCTACTAACCTCTCCAGAAACGTTGAGAGGAGCAATAGAAGACATGGGATTTGATGCTACCT TGTCAGACACGAATGAGCCGTTGGTAGTAATAGCTCAGCCTTCATCGGAAATGCCGCTTTTGACTTCAAC TAATGAATTTTATACTAAAGGGATGACACCAGTTCAAGACAAGGAGGAAGGAAAGAATTCATCTAAGTGT TACATACAGGTCACTGGCATGACTTGCGCTTCCTGTGTAGCAAACATTGAACGGAATTTAAGGCGGGAAG AAGGAATATATTCTATACTTGTGGCCCTGATGGCTGGCAAGGCAGAAGTAAGGTATAATCCTGCTGTTAT ACAACCCCCAATGATAGCAGAGTTCATCCGAGAACTTGGATTTGGAGCCACTGTGATAGAAAATGCTGAT GAAGGAGATGGTGTTTTGGAACTTGTTGTGAGGGGAATGACGTGTGCCTCCTGCGTACATAAAATAGAGT CTAGTCTCACAAAACACAGAGGGATCCTATACTGCTCCGTGGCCCTGGCAACCAACAAAGCACATATTAA ATATGACCCAGAAATTATTGGTCCTAGAGATATTATCCATACAATTGAAAGCTTAGGTTTTGAAGCTTCT TTGGTCAAGAAGGATCGGTCAGCAAGTCACTTAGATCATAAACGAGAAATAAGACAATGGAGACGGTCTT TTCTTGTGAGTCTGTTTTTCTGTATTCCTGTAATGGGGCTGATGATATATATGATGGTTATGGACCACCA CTTTGCAACTCTTCACCATAATCAAAACATGAGTAAAGAAGAAATGATCAACCTTCATTCTTCTATGTTC CTGGAGCGCCAGATTCTTCCAGGATTGTCTGTTATGAATTTGCTGTCCTTTTTATTGTGTGTACCTGTAC AGTTTTTCGGAGGCTGGTACTTCTACATTCAGGCTTATAAAGCACTGAAGCATAAGACAGCAAATATGGA CGTACTGATTGTGCTGGCAACCACCATTGCATTTGCCTACTCTTTGATTATTCTTCTAGTTGCAATGTAT GAGAGAGCCAAAGTGAACCCTATTACTTTCTTTGACACACCCCCTATGCTGTTTGTGTTTATTGCACTAG GCCGATGGCTGGAACATATAGCAAAGGGCAAAACATCAGAGGCTCTTGCAAAGTTAATTTCACTACAAGC TACAGAAGCAACTATTGTAACTCTTGATTCTGATAATATCCTCCTCAGTGAAGAACAAGTGGATGTGGAA CTTGTACAACGTGGAGATATCATTAAAGTAGTTCCAGGAGGCAAATTTCCAGTGGATGGTCGTGTTATTG AAGGACATTCTATGGTAGATGAGTCCCTCATCACAGGGGAGGCAATGCCTGTGGCTAAGAAACCTGGCAG CACAGTGATTGCTGGTTCCATTAACCAGAACGGGTCACTGCTTATCTGCGCAACACATGTTGGAGCAGAC ACAACCCTTTCTCAAATTGTCAAACTTGTGGAAGAGGCACAAACATCAAAGGCTCCTATCCAGCAGTTTG CAGACAAACTCAGTGGCTATTTTGTTCCTTTTATTGTTTTTGTTTCCATTGCCACCCTCTTGGTATGGAT TGTAATTGGATTTCTGAATTTTGAAATTGTGGAAACCTACTTTCCTGGCTACAATAGAAGTATCTCCCGA ACAGAAACGATAATACGATTTGCTTTCCAAGCCTCTATCACAGTTCTGTGTATTGCATGTCCCTGTTCAC TGGGACTGGCCACTCCAACTGCTGTGATGGTGGGTACAGGAGTAGGTGCTCAAAATGGCATACTAATAAA AGGTGGAGAGCCATTGGAGATGGCTCATAAGGTAAAGGTAGTGGTATTTGATAAGACTGGAACCATTACT CACGGAACCCCAGTGGTGAATCAAGTAAAGGTTCTAACTGAAAGTAACAGAATATCACACCATAAAATCT TGGCCATTGTGGGAACTGCTGAAAGTAACAGTGAACACCCTCTAGGAACAGCCATAACCAAATATTGCAA ACAGGAGCTGGACACTGAAACCTTGGGTACCTGCATAGATTTCCAGGTTGTGCCAGGCTGTGGTATTAGC TGTAAAGTCACCAATATTGAAGGCTTGCTACATAAGAATAACTGGAATATAGAGGACAATAATATTAAAA ATGCATCCCTGGTTCAAATTGATGCCAGTAATGAACAGTCATCAACTTCGTCTTCCATGATTATTGATGC CCAGATCTCAAATGCTCTTAATGCTCAGCAGTATAAAGTCCTCATTGGTAACCGGGAGTGGATGATTAGA AATGGTCTTGTCATTAATAACGATGTAAATGATTTCATGACTGAACATGAGAGAAAAGGTCGGACTGCTG TATTAGTAGCAGTTGATGATGAGCTGTGTGGCTTGATAGCCATTGCAGACACAGTGAAGCCTGAAGCAGA ACTGGCTATCCATATTCTGAAATCTATGGGCTTAGAAGTAGTTCTGATGACTGGAGACAACAGTAAAACA GCTAGATCTATTGCTTCTCAGGTTGGCATTACTAAGGTGTTTGCTGAAGTTCTACCTTCTCACAAGGTTG CTAAAGTGAAGCAACTTCAAGAGGAGGGGAAACGGGTAGCAATGGTGGGAGATGGAATCAATGACTCCCC AGCTCTGGCAATGGCTAATGTGGGAATTGCTATTGGCACAGGCACAGATGTAGCCATTGAAGCAGCTGAT GTGGTTTTGATAAGGAATGATCTTCTGGATGTAGTGGCAAGTATTGACTTATCAAGAGAGACAGTCAAGA GGATTCGGATAAATTTTGTCTTTGCTCTAATTTATAATCTGGTTGGAATTCCCATAGCTGCTGGAGTTTT TATGCCCATTGGTTTGGTTTTGCAGCCCTGGATGGGATCTGCAGCAATGGCTGCTTCATCTGTTTCTGTA GTACTTTCTTCTCTCTTCCTTAAACTTTACAGGAAACCAACTTACGAGAGTTATGAACTGCCTGCCCGGA GCCAGATAGGACAGAAGAGTCCTTCAGAAATCAGCGTTCATGTTGGAATAGATGATACCTCAAGGAATTC TCCTAAACTGGGTTTGCTGGACCGGATTGTTAATTATAGCAGAGCCTCTATAAACTCACTACTGTCTGAT AAACGCTCCCTAAACAGTGTTGTTACCAGTGAACCTGACAAGCACTCACTCCTGGTGGGAGACTTCAGGG AAGATGATGACACTGCATTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224789 representing NM_000052
Red=Cloning site Green=Tags(s) MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEEKNATIIYDPKLQTPKTLQEAIDDMG FDAVIHNPDPLPVLTDTLFLTVTASLTLPWDHIQSTLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIK ELVPELSLDTGTLEKKSGACEDHSMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQE ATIVYQPHLISVEEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTNDSTAT FIIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKAIEAVSPGLYRVSITSE VESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCVQSIEGVISKKPGVKSIRVSLANSNGTVE YDPLLTSPETLRGAIEDMGFDATLSDTNEPLVVIAQPSSEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKC YIQVTGMTCASCVANIERNLRREEGIYSILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENAD EGDGVLELVVRGMTCASCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIESLGFEAS LVKKDRSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLHHNQNMSKEEMINLHSSMF LERQILPGLSVMNLLSFLLCVPVQFFGGWYFYIQAYKALKHKTANMDVLIVLATTIAFAYSLIILLVAMY ERAKVNPITFFDTPPMLFVFIALGRWLEHIAKGKTSEALAKLISLQATEATIVTLDSDNILLSEEQVDVE LVQRGDIIKVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLICATHVGAD TTLSQIVKLVEEAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISR TETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVVFDKTGTIT HGTPVVNQVKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQELDTETLGTCIDFQVVPGCGIS CKVTNIEGLLHKNNWNIEDNNIKNASLVQIDASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIR NGLVINNDVNDFMTEHERKGRTAVLVAVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKT ARSIASQVGITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAAD VVLIRNDLLDVVASIDLSRETVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAMAASSVSV VLSSLFLKLYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGLLDRIVNYSRASINSLLSD KRSLNSVVTSEPDKHSLLVGDFREDDDTAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000052 |
| ORF Size | 4500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000052.5 |
| RefSeq Size | 8492 bp |
| RefSeq ORF | 4503 bp |
| Locus ID | 538 |
| UniProt ID | Q04656 |
| Domains | E1-E2_ATPase, Hydrolase, HMA |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed. [provided by RefSeq, Aug 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224789 | ATP7A (Myc-DDK-tagged)-Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A). Note: ORF is codon optimized |
CNY 15,392.00 |
|
| RC224789L3 | Lenti ORF clone of Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A), Myc-DDK-tagged. Note: ORF is codon optimized |
CNY 16,910.00 |
|
| SC308719 | ATP7A (untagged)-Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A) |
CNY 15,408.00 |
