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DUOX1 (NM_017434) Human Tagged ORF Clone
CAT＃: RG223975
- TrueORF®
DUOX1 (tGFP-tagged) - Human dual oxidase 1 (DUOX1), transcript variant 1
ORF Plasmid: DDK
"NM_017434" in other vectors (6)
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CNY 17,010.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | LNOX1; NOXEF1; THOX1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223975 representing NM_017434
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTCTGCCTGGCTCTAGCATGGACACTTCTGGTTGGGGCATGGACCCCTCTGGGAGCTCAGAACC CCATTTCGTGGGAGGTGCAGCGATTTGATGGGTGGTACAACAACCTCATGGAGCACAGATGGGGCAGCAA AGGCTCCCGGCTGCAGCGCCTGGTCCCAGCCAGCTATGCAGATGGCGTGTACCAGCCCTTGGGAGAACCC CACCTGCCCAACCCCCGAGACCTTAGCAACACCATCTCAAGGGGCCCTGCAGGGCTGGCCTCCCTGAGAA ACCGCACAGTGTTGGGGGTCTTCTTTGGCTATCACGTGCTTTCAGACCTGGTGAGCGTGGAAACTCCCGG CTGCCCCGCCGAGTTCCTCAACATTCGCATCCCGCCCGGAGACCCCATGTTCGACCCCGACCAGCGCGGG GACGTGGTGCTGCCCTTCCAGAGAAGCCGCTGGGACCCCGAGACCGGACGGAGTCCCAGCAATCCCCGGG ACCCGGCCAACCAGGTGACGGGCTGGCTGGACGGCAGCGCCATCTATGGTTCCTCGCATTCCTGGAGCGA CGCGCTGCGGAGCTTCTCCAGGGGACAGCTGGCGTCGGGGCCCGACCCCGCTTTTCCCCGAGACTCGCAG AACCCCCTGCTCATGTGGGCGGCGCCCGACCCCGCCACCGGGCAGAACGGGCCCCGGGGGCTGTACGCCT TCGGGGCAGAGAGAGGGAACCGGGAACCCTTCCTGCAGGCGCTGGGCCTGCTCTGGTTCCGCTACCACAA CCTGTGGGCGCAGAGGCTGGCCCGCCAGCACCCAGACTGGGAGGACGAGGAGCTGTTCCAGCACGCACGC AAGAGGGTCATCGCCACCTACCAGAACATCGCTGTGTATGAGTGGCTGCCCAGCTTCCTGCAGAAAACAC TCCCGGAGTATACAGGATACCGGCCATTTCTGGACCCCAGCATCTCCTCAGAGTTCGTGGCGGCCTCTGA GCAGTTCCTGTCCACCATGGTGCCCCCTGGCGTCTACATGAGAAATGCCAGCTGCCACTTCCAGGGGGTC ATCAATCGGAACTCAAGTGTCTCCAGAGCTCTCCGGGTCTGCAACAGCTACTGGAGCCGTGAGCACCCAA GCCTACAAAGTGCTGAAGATGTGGATGCACTGCTGCTGGGCATGGCCTCCCAGATCGCAGAGCGAGAGGA CCATGTGTTGGTTGAAGATGTGCGGGATTTCTGGCCTGGGCCACTGAAGTTTTCCCGCACAGACCACCTG GCCAGCTGCCTGCAGCGGGGCCGGGATCTGGGCCTGCCCTCTTACACCAAGGCCAGGGCAGCACTGGGCT TGTCTCCCATTACCCGCTGGCAGGACATCAACCCTGCACTCTCCCGGAGCAATGACACTGTACTGGAGGC CACAGCTGCCCTGTACAACCAGGACTTATCCTGGCTAGAGCTGCTCCCTGGGGGACTCCTGGAGAGCCAC CGGGACCCTGGACCTCTGTTCAGCACCATCGTCCTTGAACAATTTGTGCGGCTACGGGATGGTGACCGCT ACTGGTTTGAGAACACCAGGAATGGGCTGTTCTCCAAGAAGGAGATTGAAGAAATCCGAAATACCACCCT GCAGGACGTGCTGGTCGCTGTTATCAACATTGACCCCAGTGCTCTGCAGCCCAATGTCTTTGTCTGGCAT AAAGGAGACCCCTGTCCGCAGCCGAGACAGCTCAGCACTGAAGGCCTGCCAGCGTGTGCTCCCTCTGTTG TTCGTGACTATTTTGAGGGCAGTGGATTTGGCTTCGGGGTCACCATCGGGACCCTCTGTTGCTTCCCTTT GGTGAGCCTGCTCAGTGCCTGGATTGTTGCCCGGCTCCGGATGAGAAATTTCAAGAGGCTCCAGGGCCAG GACCGCCAGAGCATCGTGTCTGAGAAGCTCGTGGGAGGCATGGAAGCTTTGGAATGGCAAGGCCACAAGG AGCCCTGCCGGCCCGTGCTTGTGTACCTGCAGCCCGGGCAGATCCGTGTGGTAGATGGCAGGCTCACCGT GCTCCGCACCATCCAGCTGCAGCCTCCACAGAAGGTCAACTTCGTCCTGTCCAGCAACCGTGGACGCCGC ACTCTGCTGCTCAAGATCCCCAAGGAGTATGACCTGGTGCTGCTGTTTAACTTGGAGGAAGAGCGGCAGG CGCTGGTGGAAAATCTCCGGGGAGCTCTGAAGGAGAGCGGGTTGAGCATCCAGGAGTGGGAGCTGCGGGA GCAGGAGCTGATGAGAGCAGCTGTGACACGGGAGCAGCGGAGGCACCTCCTGGAGACCTTTTTCAGGCAC CTTTTCTCCCAGGTGCTGGACATCAACCAGGCCGACGCAGGGACCCTGCCCCTGGACTCCTCCCAGAAGG TGCGGGAGGCCCTGACCTGTGAGCTGAGCAGGGCCGAGTTTGCCGAGTCCCTGGGCCTCAAGCCCCAGGA CATGTTTGTGGAGTCCATGTTCTCTCTGGCTGACAAGGATGGCAATGGCTACCTGTCCTTCCGAGAGTTC CTGGACATCCTGGTGGTCTTCATGAAAGGCTCTCCTGAGGAAAAGTCTCGCCTTATGTTCCGCATGTACG ACTTTGATGGGAATGGCCTCATTTCCAAGGATGAGTTCATCAGGATGCTGAGATCCTTCATCGAGATCTC CAACAACTGCCTGTCCAAGGCCCAGCTGGCTGAGGTGGTGGAGTCCATGTTCCGGGAGTCGGGATTCCAG GACAAGGAGGAACTGACATGGGAAGATTTTCACTTCATGCTGCGGGACCACAATAGCGAGCTCCGCTTCA CGCAGCTCTGTGTCAAAGGGGTGGAGGTGCCTGAAGTCATCAAGGACCTCTGCCGGCGAGCCTCCTACAT CAGCCAGGATATGATCTGTCCCTCTCCCAGAGTGAGTGCCCGCTGTTCCCGCAGCGACATTGAGACTGAG TTGACACCTCAGAGACTGCAGTGCCCCATGGACACAGACCCTCCCCAGGAGATTCGGCGGAGGTTTGGCA AGAAGGTAACGTCATTCCAGCCCTTGCTGTTCACTGAGGCGCACCGAGAGAAGTTCCAACGCAGCTGTCT CCACCAGACGGTGCAACAGTTCAAGCGCTTCATTGAGAACTACCGGCGCCACATCGGCTGCGTGGCCGTG TTCTACGCCATCGCTGGGGGGCTTTTCCTGGAGAGGGCCTACTACTACGCCTTTGCCGCACATCACACGG GCATCACAGACACCACCCGCGTGGGAATCATCCTGTCGCGGGGCACAGCAGCCAGCATCTCTTTCATGTT CTCCTACATCTTGCTCACCATGTGCCGCAACCTCATCACCTTCCTGCGAGAAACCTTCCTCAACCGCTAC GTGCCCTTCGACGCCGCCGTGGACTTCCATCGCCTCATTGCCTCCACCGCCATCGTCCTCACAGTCTTAC ACAGTGTGGGCCATGTGGTGAATGTGTACCTGTTCTCCATCAGCCCCCTCAGCGTCCTCTCTTGCCTCTT TCCTGGCCTCTTCCATGATGATGGGTCTGAGCTCCCCCAGAAGTATTACTGGTGGTTCTTCCAGACCGTA CCAGGCCTCACGGGGGTTGTGCTGCTCCTGATCCTGGCCATCATGTATGTCTTTGCCTCCCACCACTTCC GCCGCCGCAGTTTCCGGGGCTTCTGGCTGACCCACCACCTCTACATCCTGCTCTATGTCCTGCTCATCAT CCATGGTAGCTTTGCCCTGATCCAGCTGCCCCGTTTCCACATCTTCTTCCTGGTCCCAGCAATCATCTAT GGGGGCGACAAGCTGGTGAGCCTGAGCCGGAAGAAGGTGGAGATCAGCGTGGTGAAGGCGGAGCTGCTGC CCTCAGGAGTGACCCACCTGCGGTTCCAGCGGCCCCAGGGCTTTGAGTACAAGTCAGGGCAGTGGGTGCG GATCGCTTGCCTGGCTCTGGGGACCACCGAGTACCACCCCTTCACACTGACCTCTGCGCCCCATGAGGAC ACGCTTAGCCTGCACATCCGGGCAGCAGGGCCCTGGACCACTCGCCTCAGGGAGATCTACTCAGCCCCGA CGGGTGACAGATGTGCCAGATACCCAAAGCTGTACCTTGATGGACCATTTGGAGAGGGCCACCAGGAGTG GCATAAGTTTGAGGTGTCAGTGTTAGTGGGAGGGGGCATTGGGGTCACCCCTTTTGCCTCCATCCTCAAA GACCTGGTCTTCAAGTCATCCGTCAGCTGCCAAGTGTTCTGTAAGAAGATCTACTTCATCTGGGTGACGC GGACCCAGCGTCAGTTTGAGTGGCTGGCTGACATCATCCGAGAGGTGGAGGAGAATGACCACCAGGACCT GGTGTCTGTGCACATCTACATCACCCAGCTGGCTGAGAAGTTCGACCTCAGGACCACTATGCTGTACATC TGTGAGCGGCACTTCCAGAAGGTTCTGAACCGGAGTCTATTCACAGGCCTGCGCTCCATCACCCACTTTG GCCGTCCCCCCTTTGAGCCCTTCTTCAACTCCCTGCAGGAGGTCCACCCCCAGGTCCGGAAGATCGGGGT GTTTAGCTGTGGCCCCCCTGGCATGACCAAGAATGTGGAAAAGGCCTGTCAGCTCATCAACAGGCAGGAC CGGACTCACTTCTCCCACCATTATGAGAACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223975 representing NM_017434
Red=Cloning site Green=Tags(s) MGFCLALAWTLLVGAWTPLGAQNPISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLGEP HLPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPMFDPDQRG DVVLPFQRSRWDPETGRSPSNPRDPANQVTGWLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQ NPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHAR KRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSEFVAASEQFLSTMVPPGVYMRNASCHFQGV INRNSSVSRALRVCNSYWSREHPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHL ASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESH RDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWH KGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQ DRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRR TLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRH LFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREF LDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQ DKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETE LTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAV FYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRY VPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTV PGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIY GGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHED TLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILK DLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYI CERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQD RTHFSHHYENF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017434 |
| ORF Size | 4653 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017434.5 |
| RefSeq Size | 5693 bp |
| RefSeq ORF | 4656 bp |
| Locus ID | 53905 |
| UniProt ID | Q9NRD9 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes proteins encoded by this gene and the similar DUOX2 gene. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. This protein generates hydrogen peroxide and thereby plays a role in the activity of thyroid peroxidase, lactoperoxidase, and in lactoperoxidase-mediated antimicrobial defense at mucosal surfaces. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223975 | DUOX1 (Myc-DDK-tagged)-Human dual oxidase 1 (DUOX1), transcript variant 1 |
CNY 15,920.00 |
|
| RC223975L1 | Lenti-ORF clone of DUOX1 (Myc-DDK-tagged)-Human dual oxidase 1 (DUOX1), transcript variant 1 |
CNY 18,320.00 |
|
| RC223975L2 | Lenti-ORF clone of DUOX1 (mGFP-tagged)-Human dual oxidase 1 (DUOX1), transcript variant 1 |
CNY 17,390.00 |
|
| RC223975L3 | Lenti-ORF clone of DUOX1 (Myc-DDK-tagged)-Human dual oxidase 1 (DUOX1), transcript variant 1 |
CNY 17,390.00 |
|
| RC223975L4 | Lenti-ORF clone of DUOX1 (mGFP-tagged)-Human dual oxidase 1 (DUOX1), transcript variant 1 |
CNY 17,390.00 |
|
| SC304464 | DUOX1 (untagged)-Human dual oxidase 1 (DUOX1), transcript variant 1 |
CNY 15,928.00 |
