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RTN3 (NM_201428) Human Tagged ORF Clone
CAT＃: RG220992
- TrueORF®
RTN3 (tGFP-tagged) - Human reticulon 3 (RTN3), transcript variant 2
ORF Plasmid: DDK
"NM_201428" in other vectors (4)
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CNY 11,120.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ASYIP; HAP; NSPL2; NSPLII; RTN3-A1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220992 representing NM_201428
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCGTCGGCGGCCACTCAGTCCCATTCCATCTCCTCGTCGTCCTTCGGAGCCGAGCCGTCCG CGCCCGGCGGCGGCGGGAGCCCAGGAGCCTGCCCCGCCCTGGGGACGAAGAGCTGCAGCTCCTCCTGTGC GGAGGGATTGAGCTCTCTTTGCTCTGATGAGCCATCTTCAGAAATTATGACTTCTTCCTTTCTTTCATCT TCTGAAATACATAACACTGGCCTTACAATACTACATGGAGAAAAAAGCCATGTGTTAGGGAGCCAGCCTA TTTTAGCCAAAGAAGGAAAAGACCACTTGGATCTTCTAGATATGAAAAAGATGGAAAAGCCTCAGGGGAC CAGCAACAACGTATCAGACTCTTCAGTTTCTCTTGCAGCAGGAGTTCATTGTGACCGTCCTTCTATTCCA GCCAGTTTCCCAGAGCATCCTGCTTTTCTCTCAAAGAAAATTGGTCAAGTGGAAGAGCAAATAGATAAAG AGACCAAGAACCCAAATGGGGTATCAAGTAGGGAGGCTAAAACTGCATTGGATGCTGATGACAGATTCAC TTTGCTGACAGCCCAGAAACCACCTACTGAGTACTCTAAGGTAGAAGGCATTTATACATATTCTTTGTCT CCATCCAAAGTTTCAGGAGATGATGTTATTGAAAAGGATTCCCCTGAATCACCATTTGAAGTAATTATTG ACAAAGCAGCATTTGACAAAGAATTTAAAGACTCATATAAGGAGAGCACAGATGATTTTGGTAGCTGGTC TGTGCACACTGATAAAGAATCATCCGAAGACATTTCAGAGACTAATGACAAGCTTTTTCCACTGAGAAAT AAAGAGGCAGGACGTTACCCAATGTCTGCATTGCTCAGTAGGCAGTTTTCACACACAAATGCAGCACTGG AAGAGGTGTCCAGATGCGTGAATGATATGCATAACTTTACTAACGAAATACTGACTTGGGATCTGGTTCC CCAAGTGAAACAACAGACCGATAAATCTTCTGACTGCATCACAAAAACTACAGGACTTGACATGAGTGAA TATAATTCAGAAATTCCAGTTGTAAATCTTAAAACTAGCACTCATCAGAAAACTCCTGTATGTTCTATTG ATGGGAGCACTCCCATCACTAAATCAACAGGTGATTGGGCAGAAGCATCTCTCCAGCAAGAAAATGCTAT TACTGGAAAACCTGTACCTGACTCTTTGAATTCCACAAAAGAATTCAGTATCAAAGGTGTGCAAGGCAAT ATGCAGAAACAGGATGACACACTTGCAGAATTACCTGGATCTCCACCTGAGAAATGTGACTCTTTGGGTT CTGGAGTGGCCACAGTGAAAGTGGTTTTACCTGATGACCACCTGAAAGATGAAATGGACTGGCAGAGCTC TGCATTGGGAGAAATCACAGAAGCTGATAGTTCTGGTGAGTCTGATGACACAGTAATAGAGGACATCACA GCAGATACATCATTTGAAAATAACAAAATTCAGGCTGAAAAACCTGTTTCCATTCCAAGTGCTGTTGTAA AAACAGGTGAAAGAGAAATCAAAGAGATTCCCAGTTGTGAGAGAGAAGAAAAAACATCTAAAAACTTTGA AGAATTGGTCAGTGACTCTGAGCTGCATCAAGATCAGCCTGATATTCTTGGAAGGAGTCCAGCTAGTGAG GCAGCATGTTCAAAAGTACCCGATACGAATGTCTCCTTAGAAGATGTGAGTGAAGTTGCTCCTGAAAAGC CTATTACTACTGAGAACCCCAAACTTCCTTCAACAGTGTCTCCAAATGTTTTTAATGAGACAGAATTCTC ATTAAATGTGACAACATCTGCCTATTTGGAGTCATTACATGGGAAAAATGTTAAACATATAGATGATTCC TCCCCAGAGGACCTGATAGCAGCCTTTACAGAAACCAGAGATAAAGGAATAGTAGATAGTGAAAGAAATG CTTTTAAAGCAATATCAGAGAAGATGACAGACTTTAAAACAACTCCTCCTGTAGAAGTCTTACATGAAAA TGAGTCCGGTGGTTCTGAAATTAAAGACATTGGAAGCAAATACAGTGAACAAAGCAAAGAAACAAATGGA AGTGAGCCTCTAGGTGTTTTCCCTACCCAAGGTACTCCAGTAGCATCTCTTGACTTAGAACAAGAACAGC TCACAATTAAGGCTCTTAAAGAATTAGGTGAAAGACAGGTTGAGAAGTCAACTTCTGCACAGCGTGACGC AGAATTGCCTTCTGAAGAAGTACTGAAGCAAACTTTCACATTTGCTCCAGAATCTTGGCCACAGAGATCA TATGACATCCTAGAACGTAATGTCAAGAATGGATCTGATCTTGGGATTTCCCAGAAGCCCATCACTATCA GAGAAACTACTAGGGTAGATGCTGTTTCCAGCCTTAGCAAGACTGAATTGGTAAAAAAGCATGTCCTAGC AAGACTTCTGACAGACTTCTCAGTGCACGATCTGATTTTCTGGAGAGATGTGAAGAAGACTGGGTTTGTC TTTGGCACCACGCTGATCATGCTGCTTTCCCTGGCAGCTTTCAGTGTCATCAGTGTGGTTTCTTACCTCA TCCTGGCTCTTCTCTCTGTCACCATCAGCTTCAGGATCTACAAGTCCGTCATCCAAGCTGTACAGAAGTC AGAAGAAGGCCATCCATTCAAAGCCTACCTGGACGTAGACATTACTCTGTCCTCAGAAGCTTTCCATAAT TACATGAATGCTGCCATGGTGCACATCAACAGGGCCCTGAAACTCATTATTCGTCTCTTTCTGGTAGAAG ATCTGGTTGACTCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAACGG AATCACCCTTCTAATTCTTGCTGAACTGCTCATTTTCAGTGTCCCGATTGTCTATGAGAAGTACAAGACC CAGATTGATCACTATGTTGGCATCGCCCGAGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAAC TCCCTGGAATCGCCAAAAAAAAGGCAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220992 representing NM_201428
Red=Cloning site Green=Tags(s) MAEPSAATQSHSISSSSFGAEPSAPGGGGSPGACPALGTKSCSSSCAEGLSSLCSDEPSSEIMTSSFLSS SEIHNTGLTILHGEKSHVLGSQPILAKEGKDHLDLLDMKKMEKPQGTSNNVSDSSVSLAAGVHCDRPSIP ASFPEHPAFLSKKIGQVEEQIDKETKNPNGVSSREAKTALDADDRFTLLTAQKPPTEYSKVEGIYTYSLS PSKVSGDDVIEKDSPESPFEVIIDKAAFDKEFKDSYKESTDDFGSWSVHTDKESSEDISETNDKLFPLRN KEAGRYPMSALLSRQFSHTNAALEEVSRCVNDMHNFTNEILTWDLVPQVKQQTDKSSDCITKTTGLDMSE YNSEIPVVNLKTSTHQKTPVCSIDGSTPITKSTGDWAEASLQQENAITGKPVPDSLNSTKEFSIKGVQGN MQKQDDTLAELPGSPPEKCDSLGSGVATVKVVLPDDHLKDEMDWQSSALGEITEADSSGESDDTVIEDIT ADTSFENNKIQAEKPVSIPSAVVKTGEREIKEIPSCEREEKTSKNFEELVSDSELHQDQPDILGRSPASE AACSKVPDTNVSLEDVSEVAPEKPITTENPKLPSTVSPNVFNETEFSLNVTTSAYLESLHGKNVKHIDDS SPEDLIAAFTETRDKGIVDSERNAFKAISEKMTDFKTTPPVEVLHENESGGSEIKDIGSKYSEQSKETNG SEPLGVFPTQGTPVASLDLEQEQLTIKALKELGERQVEKSTSAQRDAELPSEEVLKQTFTFAPESWPQRS YDILERNVKNGSDLGISQKPITIRETTRVDAVSSLSKTELVKKHVLARLLTDFSVHDLIFWRDVKKTGFV FGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHN YMNAAMVHINRALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKT QIDHYVGIARDQTKSIVEKIQAKLPGIAKKKAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_201428 |
| ORF Size | 3039 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_201428.3 |
| RefSeq Size | 4919 bp |
| RefSeq ORF | 3042 bp |
| Locus ID | 10313 |
| UniProt ID | O95197 |
| Protein Families | Transmembrane |
| Gene Summary | This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12. [provided by RefSeq, May 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220992 | RTN3 (Myc-DDK-tagged)-Human reticulon 3 (RTN3), transcript variant 2 |
CNY 10,070.00 |
|
| RC220992L3 | Lenti-ORF clone of RTN3 (Myc-DDK-tagged)-Human reticulon 3 (RTN3), transcript variant 2 |
CNY 11,970.00 |
|
| RC220992L4 | Lenti-ORF clone of RTN3 (mGFP-tagged)-Human reticulon 3 (RTN3), transcript variant 2 |
CNY 11,970.00 |
|
| SC315070 | RTN3 (untagged)-Human reticulon 3 (RTN3), transcript variant 2 |
CNY 19,570.00 |
