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ATG14L (ATG14) (NM_014924) Human Tagged ORF Clone
CAT＃: RG220325
- TrueORF®
ATG14 (tGFP-tagged) - Human ATG14 autophagy related 14 homolog (S. cerevisiae) (ATG14)
ORF Plasmid: DDK
"NM_014924" in other vectors (6)
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CNY 7,088.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ATG14L; BARKOR; KIAA0831 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220325 representing NM_014924
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTCCCAGTGGGAAGGGAGCCCGGGCGCTGGAGGCTCCTGGCTGCGGGCCCCGGCCGCTCGCCC GGGACCTGGTGGACTCCGTGGACGATGCGGAGGGGCTGTACGTGGCTGTGGAGCGCTGCCCGCTGTGCAA CACTACCCGCCGGCGGCTGACCTGCGCCAAATGCGTTCAGAGCGGCGATTTCGTCTACTTCGACGGCCGC GACCGGGAGAGGTTTATCGACAAGAAGGAAAGGTTAAGCCGACTTAAGAGCAAGCAAGAAGAATTTCAGA AAGAAGTGTTAAAAGCTATGGAAGGAAAATGGATAACAGATCAGTTGAGATGGAAAATAATGTCCTGCAA GATGAGGATTGAACAGTTAAAACAAACAATATGTAAAGGAAATGAAGAAATGGAGAAAAATTCTGAAGGC CTTCTCAAAACCAAGGAAAAGAATCAGAAGCTTTACAGTCGAGCACAACGGCACCAAGAGAAAAAGGAGA AGATTCAGAGGCATAATCGCAAACTTGGTGACCTGGTAGAAAAAAAGACCATTGACTTAAGAAGTCATTA TGAGCGTCTGGCAAATCTTCGACGATCCCATATATTAGAGCTCACCTCTGTCATTTTTCCAATCGAGGAA GTAAAGACGGGTGTGAGAGACCCCGCAGATGTGTCTTCAGAGAGTGACAGTGCCATGACCTCCAGCACTG TGAGCAAGCTTGCTGAAGCCCGGAGGACAACTTACCTCTCAGGACGATGGGTCTGTGACGATCACAACGG AGACACCAGCATTAGCATTACAGGGCCTTGGATTAGCCTCCCTAACAATGGGGACTACTCTGCCTACTAC AGCTGGGTGGAGGAGAAGAAAACAACCCAGGGGCCTGACATGGAGCAGAGTAACCCTGCCTACACCATCA GTGCTGCGCTGTGCTATGCAACTCAGCTGGTCAACATTCTGTCTCATATACTTGATGTAAATCTTCCCAA AAAGCTCTGCAACAGTGAATTTTGTGGCGAAAATCTAAGCAAGCAGAAATTTACTCGAGCAGTGAAGAAA CTGAATGCAAATATTCTTTACCTTTGTTTTTCTCAGCATGTAAATTTAGATCAATTACAACCACTGCATA CCCTCAGGAATCTAATGTACCTGGTCAGTCCAAGCTCTGAACACCTAGGCAGGTCAGGGCCCTTTGAAGT ACGAGCAGACCTTGAGGAGTCCATGGAATTTGTGGATCCCGGAGTTGCTGGAGAATCAGATGAGAGCGGA GATGAGCGCGTCAGCGATGAAGAAACCGACCTGGGCACAGACTGGGAGAACTTGCCTAGTCCCCGGTTTT GTGATATCCCTTCCCAGTCTGTGGAAGTCTCCCAGAGTCAGAGCACCCAGGCGTCCCCACCCATCGCGAG CAGCAGTGCAGGTGGGATGATCTCCTCTGCAGCAGCCTCGGTGACCTCCTGGTTTAAAGCTTACACTGGA CACCGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220325 representing NM_014924
Red=Cloning site Green=Tags(s) MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGR DRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEG LLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEE VKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYY SWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKK LNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESG DERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTG HR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014924 |
| ORF Size | 1476 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014924.5 |
| RefSeq Size | 4742 bp |
| RefSeq ORF | 1479 bp |
| Locus ID | 22863 |
| UniProt ID | Q6ZNE5 |
| Gene Summary | Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604). Modulates the hepatic lipid metabolism (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220325 | ATG14 (Myc-DDK-tagged)-Human ATG14 autophagy related 14 homolog (S. cerevisiae) (ATG14) |
CNY 5,488.00 |
|
| RC220325L1 | Lenti-ORF clone of ATG14 (Myc-DDK-tagged)-Human ATG14 autophagy related 14 homolog (S. cerevisiae) (ATG14) |
CNY 7,888.00 |
|
| RC220325L2 | Lenti-ORF clone of ATG14 (mGFP-tagged)-Human ATG14 autophagy related 14 homolog (S. cerevisiae) (ATG14) |
CNY 5,890.00 |
|
| RC220325L3 | Lenti-ORF clone of ATG14 (Myc-DDK-tagged)-Human ATG14 autophagy related 14 homolog (S. cerevisiae) (ATG14) |
CNY 5,890.00 |
|
| RC220325L4 | Lenti-ORF clone of ATG14 (mGFP-tagged)-Human ATG14 autophagy related 14 homolog (S. cerevisiae) (ATG14) |
CNY 5,890.00 |
|
| SC313704 | ATG14 (untagged)-Human ATG14 autophagy related 14 homolog (S. cerevisiae) (ATG14) |
CNY 7,220.00 |
