NPY4R (NM_005972) Human Tagged ORF Clone

CAT#: RG219358

  • TrueORF®

PPYR1 (tGFP-tagged) - Human pancreatic polypeptide receptor 1 (PPYR1)

ORF Plasmid: DDK tGFP



  "NM_005972" in other vectors (6)

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CNY 7,088.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-NPY4R Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms NPY4-R; PP1; PPYR1; Y4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG219358 representing NM_005972
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACACCTCTCACCTCCTGGCCTTGCTGCTCCCAAAATCTCCACAAGGTGAAAACAGAAGCAAACCCC
TGGGCACCCCATACAACTTCTCTGAACATTGCCAGGATTCCGTGGACGTGATGGTCTTCATCGTCACTTC
CTACAGCATTGAGACTGTCGTGGGGGTCCTGGGTAACCTCTGCCTGATGTGTGTGACTGTGAGGCAGAAG
GAGAAAGCCAACGTGACCAACCTGCTTATCGCCAACCTGGCCTTCTCTGACTTCCTCATGTGCCTCCTCT
GCCAGCCGCTGACCTCCGTCTACACCATCATGGACTACTGGATCTTTGGAGAGACCCTCTGCAAGATGTC
GGCCTTCATCCAGTGCATGTCGGTGACGGTCTCCATCCTCTCGCTCGTCCTCGTGGCCCTGGAGAGGCAT
CAGCTCATCATCAACCCAACAGGCTGGAAGCCCAGCATCTCACAGGCCTACCTGGGGATTGTGCTCATCT
GGGTCATTGCCTGTGTCCTCTCCCTGCCCTTCCTGGCCAACAGCATCCTGGAGAATGTCTTCCACAAGAA
CCACTCCAAGGCTCTGGAGTTCCTGGCGGATAAGGTGGTCTGTACCGAGTCCTGGCCACTGGCTCACCAC
CGCACCATCTACACCACCTTCCTGCTCCTCTTCCAGTACTGCCTCCCACTGGGCTTCATCTTGGTCTGTT
ATGCACGCATCTACCGGCGCCTGCAGAGGCAGGGGCGCGTGTTTCACAAGGGCACCTACAGCTTGCGAGC
TGGGCACATGAAGCAGGTCAATGTGGTGCTGGTGGTGATGGTGGTGGCCTTTGCCGTGCTCTGGCTGCCT
CTGCATGTGTTCAACAGCCTGGAAGACTGGCACCATGAGGCCATCCCCATCTGCCATGGGAACCTCATCT
TCTTAGTGTGCCACTTGCTTGCCATGGCCTCCACCTGTGTCAACCCATTCATCTATGGCTTTCTCAACAC
CAACTTCAAGAAGGAGATCAAGGCCCTGGTGCTGACTTGCCAGCAGAGCGCCCCCCTGGAGGAGTCAGAG
CATCTGCCCCTGTCCACAGTACATACGGAAGTCTCCAAAGGGTCCCTGAGGCTAAGTGGCAGGTCCAATC
CCATT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG219358 representing NM_005972
Red=Cloning site Green=Tags(s)

MNTSHLLALLLPKSPQGENRSKPLGTPYNFSEHCQDSVDVMVFIVTSYSIETVVGVLGNLCLMCVTVRQK
EKANVTNLLIANLAFSDFLMCLLCQPLTSVYTIMDYWIFGETLCKMSAFIQCMSVTVSILSLVLVALERH
QLIINPTGWKPSISQAYLGIVLIWVIACVLSLPFLANSILENVFHKNHSKALEFLADKVVCTESWPLAHH
RTIYTTFLLLFQYCLPLGFILVCYARIYRRLQRQGRVFHKGTYSLRAGHMKQVNVVLVVMVVAFAVLWLP
LHVFNSLEDWHHEAIPICHGNLIFLVCHLLAMASTCVNPFIYGFLNTNFKKEIKALVLTCQQSAPLEESE
HLPLSTVHTEVSKGSLRLSGRSNPI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005972
ORF Size 1125 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005972.3, NP_005963.2
RefSeq Size 1956 bp
RefSeq ORF 1128 bp
Locus ID 5540
UniProt ID P50391
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Gene Summary Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PP, PP (2-36) and [Ile-31, Gln-34] PP > [Pro-34] PYY > PYY and [Leu-31, Pro-34] NPY > NPY > PYY (3-36) and NPY (2-36) > PP (13-36) > PP (31-36) > NPY free acid.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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