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MSI1 (NM_002442) Human Tagged ORF Clone
CAT＃: RG215992
- TrueORF®
MSI1 (tGFP-tagged) - Human musashi homolog 1 (Drosophila) (MSI1)
ORF Plasmid: DDK
"NM_002442" in other vectors (6)
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CNY 7,088.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215992 representing NM_002442
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTGACGCGCCCCAGCCCGGCCTCGCCTCCCCGGACTCGCCGCACGACCCCTGCAAGATGTTCA TCGGGGGACTCAGTTGGCAGACTACGCAGGAAGGGCTGCGCGAATACTTCGGCCAGTTCGGGGAGGTGAA GGAGTGTCTGGTGATGCGGGACCCCCTGACCAAGAGATCCAGGGGTTTCGGCTTCGTCACTTTCATGGAC CAGGCGGGGGTGGATAAAGTGCTGGCGCAATCGCGGCACGAGCTCGACTCCAAAACAATTGACCCTAAGG TGGCCTTCCCTCGGCGAGCACAGCCCAAGATGGTGACTCGAACGAAGAAGATCTTTGTGGGGGGGCTGTC GGTGAACACCACGGTGGAGGACGTGAAGCAATATTTTGAGCAGTTTGGGAAGGTGGACGACGCCATGCTG ATGTTTGACAAAACCACCAACCGGCACCGAGGGTTCGGGTTTGTCACGTTTGAGAGTGAGGACATCGTGG AGAAAGTGTGTGAAATTCATTTTCATGAAATCAACAACAAAATGGTGGAATGTAAGAAAGCTCAGCCAAA GGAGGTGATGTCGCCAACGGGCTCAGCCCGGGGGAGGTCTCGAGTCATGCCCTACGGAATGGACGCCTTC ATGCTGGGCATCGGCATGCTGGGTTACCCAGGTTTCCAAGCCACAACCTACGCCAGCCGGAGTTATACAG GCCTCGCCCCTGGCTACACCTACCAGTTCCCCGAATTCCGTGTAGAGCGGACCCCTCTCCCGAGCGCCCC AGTCCTCCCCGAGCTTACAGCCATTCCTCTCACTGCCTACGGACCAATGGCGGCGGCAGCGGCGGCAGCG GCTGTGGTTCGAGGGACAGGCTCTCACCCCTGGACGATGGCTCCCCCTCCAGGTTCGACTCCCAGCCGCA CAGGGGGCTTCCTGGGGACCACCAGCCCCGGCCCCATGGCCGAGCTCTACGGGGCGGCCAACCAGGACTC GGGGGTCAGCAGTTACATCAGCGCCGCCAGCCCTGCCCCCAGCACCGGCTTCGGCCACAGTCTTGGGGGC CCTTTGATTGCCACAGCCTTCACCAATGGGTACCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215992 representing NM_002442
Red=Cloning site Green=Tags(s) METDAPQPGLASPDSPHDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMD QAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSVNTTVEDVKQYFEQFGKVDDAML MFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVMPYGMDAF MLGIGMLGYPGFQATTYASRSYTGLAPGYTYQFPEFRVERTPLPSAPVLPELTAIPLTAYGPMAAAAAAA AVVRGTGSHPWTMAPPPGSTPSRTGGFLGTTSPGPMAELYGAANQDSGVSSYISAASPAPSTGFGHSLGG PLIATAFTNGYH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002442 |
| ORF Size | 1086 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002442.4 |
| RefSeq Size | 2950 bp |
| RefSeq ORF | 1089 bp |
| Locus ID | 4440 |
| UniProt ID | O43347 |
| Gene Summary | This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Interplay among p21Waf1/Cip1, MUSASHI-1 and Krüppel-like factor 4 in activation of Bmi1-CreER reserve intestinal stem cells after gamma radiation-induced injury
,Orzechowska, E;Katano, T;Bialkowska, A;Yang, V;,
Sci Rep
,PubMed ID 33110120
[MSI1]
|
|
Tumor suppressive microRNA-137 negatively regulates Musashi-1 and colorectal cancer progression
,Smith, AR;Marquez, RT;Tsao, WC;Pathak, S;Roy, A;Ping, J;Wilkerson, B;Lan, L;Meng, W;Neufeld, KL;Sun, XF;Xu, L;,
Oncotarget
,PubMed ID 25940441
[MSI1]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215992 | MSI1 (Myc-DDK-tagged)-Human musashi homolog 1 (Drosophila) (MSI1) |
CNY 5,488.00 |
|
| RC215992L1 | Lenti ORF clone of Human musashi homolog 1 (Drosophila) (MSI1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC215992L2 | Lenti ORF clone of Human musashi homolog 1 (Drosophila) (MSI1), mGFP tagged |
CNY 5,890.00 |
|
| RC215992L3 | Lenti ORF clone of Human musashi homolog 1 (Drosophila) (MSI1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC215992L4 | Lenti ORF clone of Human musashi homolog 1 (Drosophila) (MSI1), mGFP tagged |
CNY 7,888.00 |
|
| SC303202 | MSI1 (untagged)-Human musashi homolog 1 (Drosophila) (MSI1) |
CNY 5,488.00 |
