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SPTBN1 (NM_178313) Human Tagged ORF Clone
CAT＃: RG212929
- TrueORF®
SPTBN1 (tGFP-tagged) - Human spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant 2
ORF Plasmid: DDK
"NM_178313" in other vectors (2)
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CNY 20,900.00
货期*
12周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | betaSpII; ELF; HEL102; SPTB2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212929 representing NM_178313
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGGAATTGCAGAGGACGTCTAGTATCTCCGGGCCGCTGTCGCCGGCGTACACGGGGCAGGTGCCTTACA ACTACAACCAGCTGGAAGGCAGATTCAAGCAGCTGCAAGATGAGCGTGAAGCCGTGCAGAAGAAGACCTT CACCAAGTGGGTCAATTCCCACCTTGCCCGTGTGTCCTGCCGGATCACAGACCTGTACACTGACCTTCGA GATGGACGGATGCTCATCAAGCTGCTGGAGGTCCTCTCTGGAGAGAGGCTGCCTAAACCCACCAAGGGAC GAATGCGCATCCACTGCTTAGAGAATGTGGACAAGGCCCTTCAGTTCCTGAAGGAGCAGAGAGTCCATCT TGAGAACATGGGGTCCCATGACATCGTGGATGGAAACCACCGGCTGACCCTTGGCCTCATCTGGACCATC ATCCTGCGCTTCCAGATCCAGGATATCAGTGTGGAAACTGAAGACAACAAAGAGAAGAAATCTGCCAAGG ATGCATTGCTGTTGTGGTGCCAGATGAAGACAGCTGGGTACCCCAATGTCAACATTCACAATTTCACCAC TAGCTGGAGGGACGGCATGGCCTTCAATGCACTGATACACAAACACCGGCCTGACCTGATAGATTTTGAC AAACTAAAGAAATCTAACGCACACTACAACCTGCAGAATGCATTTAATCTGGCAGAACAGCACCTCGGCC TCACTAAACTGTTGGACCCCGAAGACATCAGCGTGGACCATCCTGATGAGAAGTCCATAATCACTTATGT GGTGACTTATTACCACTACTTCTCTAAGATGAAGGCCTTAGCTGTTGAAGGAAAACGAATTGGAAAGGTG CTTGACAATGCTATTGAAACAGAAAAAATGATTGAAAAGTATGAATCACTTGCCTCTGACCTTCTGGAAT GGATTGAACAAACCATCATCATTCTGAACAATCGCAAATTTGCCAATTCACTGGTCGGGGTTCAACAGCA GCTTCAGGCATTCAACACTTACCGCACTGTGGAGAAACCACCCAAATTTACTGAGAAGGGGAACTTGGAA GTGCTGCTCTTCACCATTCAGAGCAAGATGAGGGCCAACAACCAGAAGGTCTACATGCCCCGGGAGGGGA AGCTCATCTCTGACATCAACAAGGCCTGGGAAAGACTGGAAAAAGCGGAACACGAAAGAGAACTGGCTTT GCGGAATGAGCTCATAAGACAGGAGAAACTGGAACAGCTCGCCCGCAGATTTGATCGCAAGGCAGCTATG AGGGAGACTTGGCTGAGCGAAAACCAGCGTCTGGTGTCTCAGGACAACTTTGGGTTTGACCTTCCTGCAG TTGAGGCCGCCACAAAAAAGCACGAGGCCATTGAGACAGACATTGCCGCATACGAGGAGCGTGTGCAGGC TGTGGTAGCCGTGGCCAGGGAGCTCGAGGCCGAGAATTACCACGACATCAAGCGCATCACAGCGAGGAAG GACAATGTCATCCGGCTCTGGGAATACCTACTGGAACTGCTCAGGGCCCGGAGACAGCGGCTCGAGATGA ACCTGGGGCTGCAGAAGATATTCCAGGAAATGCTCTACATTATGGACTGGATGGATGAAATGAAGGTGCT AGTATTGTCTCAAGACTATGGCAAACACTTACTTGGTGTGGAAGACCTGTTACAGAAGCACACCCTGGTT GAAGCAGACATTGGCATCCAGGCAGAGCGGGTGAGAGGTGTCAATGCCTCCGCCCAGAAGTTCGCAACAG ACGGGGAAGGTTACAAGCCCTGTGACCCCCAGGTGATCCGAGACCGCGTGGCCCACATGGAGTTCTGTTA TCAAGAGCTTTGCCAGCTGGCGGCTGAGCGCAGGGCCCGTCTGGAAGAGTCCCGCCGCCTCTGGAAGTTC TTCTGGGAGATGGCAGAAGAGGAAGGCTGGATACGGGAGAAGGAGAAGATCCTGTCCTCGGACGATTACG GGAAAGACCTGACCAGCGTCATGCGCCTGCTCAGCAAGCACCGGGCGTTCGAGGACGAGATGAGCGGCCG CAGTGGCCACTTTGAGCAGGCCATCAAGGAAGGCGAAGACATGATCGCGGAGGAGCACTTCGGGTCGGAG AAGATCCGTGAGAGGATCATTTACATCCGGGAGCAGTGGGCCAACCTAGAGCAGCTCTCGGCCATTCGGA AGAAGCGCCTGGAGGAGGCCTCCCTGCTGCACCAGTTCCAGGCAGATGCTGATGACATTGATGCCTGGAT GCTGGACATCCTCAAGATTGTCTCCAGCAGCGACGTGGGCCACGATGAGTATTCCACACAGTCTCTGGTC AAGAAACACAAGGACGTGGCGGAAGAGATCGCCAATTACAGGCCCACCCTTGACACGCTGCACGAACAAG CCAGCGCCCTCCCCCAGGAGCATGCCGAGTCTCCAGACGTGAGGGGCAGGCTGTCGGGCATCGAGGAGCG GTATAAGGAGGTGGCAGAGCTGACGCGGCTGCGGAAGCAGGCACTCCAGGACACTCTGGCCCTGTACAAG ATGTTCAGCGAGGCTGATGCCTGTGAGCTCTGGATCGACGAGAAGGAGCAGTGGCTCAACAACATGCAGA TCCCAGAGAAGCTGGAGGATCTGGAGGTCATCCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAA CCAGGCTTCCCGGGTTGCAGTGGTGAACCAGATTGCACGCCAGCTGATGCACAGCGGCCACCCAAGTGAG AAGGAAATCAAAGCCCAGCAGGACAAACTCAACACAAGGTGGAGCCAGTTCAGAGAACTGGTTGACAGGA AGAAGGATGCCCTCCTGTCTGCCCTGAGCATCCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTG GATTCGGGAAAAGACCAAGGTCATCGAGTCCACCCAGGACCTGGGCAATGACCTGGCTGGCGTCATGGCC CTGCAGCGCAAGCTGACCGGCATGGAGCGGGACTTGGTGGCCATTGAGGCAAAGCTGAGTGACCTGCAGA AGGAGGCGGAGAAGCTGGAGTCCGAGCACCCCGACCAGGCCCAGGCCATCCTGTCTCGGCTGGCCGAGAT CAGCGACGTGTGGGAGGAGATGAAGACCACCCTGAAAAACCGAGAGGCCTCCCTGGGAGAGGCCAGCAAG CTGCAGCAGTTCCTACGGGACTTGGACGACTTCCAGTCCTGGCTCTCTAGGACCCAGACAGCGATCGCCT CGGAGGACATGCCAAACACCCTGACCGAGGCTGAGAAGCTGCTCACGCAGCACGAGAACATCAAGAACGA GATCGACAACTACGAGGAGGACTACCAGAAGATGAGGGACATGGGCGAGATGGTCACCCAGGGGCAGACC GATGCCCAGTACATGTTTCTGCGGCAGCGGCTGCAGGCCCTGGACACTGGATGGAACGAGCTCCACAAGA TGTGGGAGAACAGACAAAATCTCCTATCCCAGTCACATGCCTACCAGCAGTTCCTCAGAGACACGAAGCA AGCCGAAGCCTTTCTTAACAACCAGGAGTATGTTCTGGCTCACACTGAAATGCCTACCACCTTGGAAGGA GCTGAAGCAGCAATTAAAAAGCAAGAGGACTTCATGACCACCATGGACGCCAATGAGGAGAAGATCAATG CTGTGGTGGAGACTGGCCGGAGGCTGGTGAGCGATGGGAACATCAACTCAGATCGCATCCAGGAGAAGGT GGACTCTATTGATGACAGACATAGGAAGAATCGTGAGACAGCCAGTGAACTTTTGATGAGGTTGAAGGAC AACAGGGATCTACAGAAATTCCTGCAAGATTGTCAAGAGCTGTCTCTCTGGATCAATGAGAAGATGCTCA CAGCCCAGGACATGTCTTACGATGAAGCCAGAAATCTGCACAGTAAATGGTTGAAGCATCAAGCATTTAT GGCAGAACTTGCATCCAACAAAGAATGGCTTGACAAAATCGAGAAGGAAGGAATGCAGCTCATTTCAGAA AAGCCTGAGACGGAAGCTGTGGTGAAGGAGAAACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAAT CCACTACCCAGACAAAGGCCCAGCGGCTCTTTGATGCAAACAAGGCCGAACTTTTCACCCAGAGCTGTGC AGATCTAGACAAATGGCTGCACGGCCTGGAGAGTCAGATTCAGTCTGATGACTATGGCAAAGACCTGACC AGTGTCAATATCCTGCTGAAAAAGCAACAGATGCTGGAGAATCAGATGGAAGTGCGGAAGAAGGAGATCG AAGAGCTCCAAAGCCAAGCCCAGGCCCTGAGTCAGGAAGGGAAGAGCACCGACGAGGTAGACAGCAAGCG CCTCACCGTGCAGACCAAGTTCATGGAGTTGCTGGAGCCCTTGAACGAGAGGAAGCATAACCTGCTGGCC TCCAAAGAGATCCATCAGTTCAACAGGGATGTGGAGGACGAGATCTTGTGGGTTGGAGAGAGGATGCCTT TGGCAACTTCCACGGATCATGGCCACAACCTCCAGACTGTGCAGCTGTTAATAAAGAAAAATCAGACCCT CCAGAAAGAAATCCAGGGGCACCAGCCTCGCATTGACGACATCTTTGAGAGGAGCCAAAACATCGTCACT GACAGCAGCAGCCTCAGCGCTGAGGCCATCAGACAGAGGCTTGCCGACCTGAAGCAGCTGTGGGGTCTCC TCATTGAGGAGACAGAGAAACGCCACAGGCGGCTGGAGGAGGCGCACAGGGCCCAGCAGTACTACTTTGA CGCTGCTGAGGCCGAAGCCTGGATGAGCGAGCAGGAGCTGTACATGATGTCAGAGGAGAAGGCCAAGGAT GAGCAGAGTGCTGTCTCCATGTTGAAGAAGCACCAGATCTTAGAACAAGCTGTGGAGGACTATGCAGAGA CCGTGCATCAGCTCTCCAAGACCAGCCGGGCCCTGGTGGCCGACAGCCATCCTGAAAGTGAGCGCATTAG CATGCGGCAGTCCAAAGTGGATAAACTGTACGCTGGTCTGAAAGACCTTGCTGAAGAGAGAAGAGGCAAG CTGGATGAGAGACACAGGTTATTCCAGCTCAACCGGGAGGTGGACGACCTGGAGCAGTGGATCGCTGAGA GGGAGGTGGTCGCAGGGTCCCATGAACTGGGACAGGACTATGAGCATGTCACGATGTTACAAGAACGATT CCGGGAGTTTGCCCGAGACACCGGGAACATTGGGCAGGAGCGCGTGGACACGGTCAATCACCTGGCAGAT GAGCTCATCAACTCTGGACATTCAGATGCCGCCACCATCGCTGAATGGAAGGATGGCCTCAATGAAGCCT GGGCCGACCTCCTGGAGCTCATTGACACAAGAACACAGATTCTTGCCGCTTCCTATGAACTGCACAAGTT TTACCACGATGCCAAGGAGATCTTTGGGCGTATACAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGG AGAGATCAGAACACAGTGGAGACCTTACAGAGAATGCACACTACATTTGAGCATGACATCCAGGCTCTGG GCACACAGGTGAGGCAGCTGCAGGAGGATGCAGCCCGCCTCCAGGCGGCCTATGCGGGTGACAAGGCCGA CGATATCCAGAAGCGCGAGAACGAGGTCCTGGAAGCCTGGAAGTCCCTCCTGGACGCCTGTGAGAGCCGC AGGGTGCGGCTGGTGGACACAGGGGACAAGTTCCGCTTCTTCAGCATGGTGCGCGACCTCATGCTCTGGA TGGAGGATGTCATCCGGCAGATCGAGGCCCAGGAGAAGCCAAGGGATGTATCATCTGTTGAACTCTTAAT GAATAATCATCAAGGCATCAAAGCTGAAATTGATGCACGTAATGACAGTTTCACAACCTGCATTGAACTT GGGAAATCCCTGTTGGCGAGAAAACACTATGCATCTGAGGAGATCAAGGAAAAATTACTGCAGTTGACGG AAAAGAGGAAAGAAATGATCGACAAGTGGGAAGACCGATGGGAATGGTTAAGACTGATTCTGGAGGTCCA TCAGTTCTCAAGAGACGCCAGTGTGGCCGAGGCCTGGCTGCTTGGACAGGAGCCGTACCTATCCAGCCGA GAGATAGGCCAGAGCGTGGACGAGGTGGAGAAGCTCATCAAGCGCCACGAGGCATTTGAAAAGTCTGCAG CAACCTGGGATGAGAGGTTCTCTGCCCTGGAAAGGCTGACTACATTGGAGTTACTGGAAGTGCGCAGACA GCAAGAGGAAGAGGAGAGGAAGAGGCGGCCGCCTTCTCCCGAGCCGAGCACGAAGGTTTCAGAGGAAGCC GAGTCCCAGCAGCAGTGGGATACTTCAAAAGGAGAACAAGTTTCCCAAAACGGTTTGCCAGCTGAACAGG GATCTCCACGGGTTAGTTACCGCTCTCAAACCTACCAAAACTACAAAAACTTTAATAGCAGACGGACAGC CAGTGACCAGCCATGGTCTGGACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212929 representing NM_178313
Red=Cloning site Green=Tags(s) MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLR DGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTI ILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFD KLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKV LDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLE VLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAM RETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARK DNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLV EADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKF FWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSE KIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLV KKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYK MFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSE KEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMA LQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASK LQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQT DAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEG AEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKD NRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISE KPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLT SVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLA SKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVT DSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKD EQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGK LDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLAD ELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELG RDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESR RVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIEL GKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSR EIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEA ESQQQWDTSKGEQVSQNGLPAEQGSPRVSYRSQTYQNYKNFNSRRTASDQPWSGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178313 |
| ORF Size | 6465 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178313.2, NP_842565.2 |
| RefSeq Size | 9091 bp |
| RefSeq ORF | 6468 bp |
| Locus ID | 6711 |
| UniProt ID | Q01082 |
| Gene Summary | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
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