RNF152 (NM_173557) Human Tagged ORF Clone
CAT#: RG211280
- TrueORF®
RNF152 (tGFP-tagged) - Human ring finger protein 152 (RNF152)
ORF Plasmid: DDK
"NM_173557" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG211280 representing NM_173557
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGCTGTCCCAGGACTCTCTGCTGGAATGTCAGATCTGTTTCAATTACTACAGCCCCCGGCGCA GGCCCAAGTTGCTGGACTGCAAGCACACCTGCTGTTCAGTGTGCCTGCAGCAGATGAGGACCAGCCAGAA GGATGTGCGGTGCCCCTGGTGCCGCGGTGTCACCAAGCTGCCTCCCGGCTTCTCCGTGTCGCAGCTCCCG GACGACCCGGAGGTCCTGGCTGTCATCGCCATTCCACACACTTCCGAACACACCCCGGTCTTCATCAAAC TTCCCAGCAATGGGTGCTACATGCTGCCCCTGCCCATCTCCAAGGAGCGTGCGCTGCTGCCCGGAGACAT GGGCTGCCGCCTGCTGCCCGGGAGCCAGCAGAAGTCCGTCACCGTGGTGACCATCCCTGCTGAACAGCAG CCTCTGCAAGGTGGGGCTCCCCAGGAGGCGGTGGAGGAGGAGCAGGACAGGCGGGGCGTGGTGAAAAGCT CCACCTGGTCGGGGGTGTGCACTGTCATCTTGGTGGCTTGCGTCTTGGTCTTCCTCCTCGGCATCGTGCT TCACAACATGTCTTGCATTTCTAAGCGCTTCACTGTGATATCCTGTGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211280 representing NM_173557
Red=Cloning site Green=Tags(s) METLSQDSLLECQICFNYYSPRRRPKLLDCKHTCCSVCLQQMRTSQKDVRCPWCRGVTKLPPGFSVSQLP DDPEVLAVIAIPHTSEHTPVFIKLPSNGCYMLPLPISKERALLPGDMGCRLLPGSQQKSVTVVTIPAEQQ PLQGGAPQEAVEEEQDRRGVVKSSTWSGVCTVILVACVLVFLLGIVLHNMSCISKRFTVISCG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_173557 |
ORF Size | 609 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_173557.3 |
RefSeq Size | 1805 bp |
RefSeq ORF | 612 bp |
Locus ID | 220441 |
UniProt ID | Q8N8N0 |
Protein Families | Transmembrane |
Gene Summary | E3 ubiquitin-protein ligase mediating 'Lys-63'-linked polyubiquitination of RRAGA in response to amino acid starvation. Thereby, regulates mTORC1 signaling and plays a role in the cellular response to amino acid availability (PubMed:25936802). Also mediates 'Lys-48'-linked polyubiquitination of target proteins and their subsequent targeting to the proteasome for degradation. Induces apoptosis when overexpressed (PubMed:21203937).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Protease-activated receptor 2 stabilizes Bcl-xL and regulates EGFR-targeted therapy response in colorectal cancer
,Li, W;Ma, Y;He, L;Li, H;Chu, Y;Jiang, Z;Zhao, X;Nie, Y;Wang, X;Wang, H;,
Cancer letters
,PubMed ID 34098062
[RNF152]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211280 | RNF152 (Myc-DDK-tagged)-Human ring finger protein 152 (RNF152) |
CNY 2,400.00 |
|
RC211280L3 | Lenti ORF clone of Human ring finger protein 152 (RNF152), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC211280L4 | Lenti ORF clone of Human ring finger protein 152 (RNF152), mGFP tagged |
CNY 5,890.00 |
|
SC108339 | RNF152 (untagged)-Human ring finger protein 152 (RNF152) |
CNY 3,990.00 |