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METRNL (NM_001004431) Human Tagged ORF Clone
CAT＃: RG210299
- TrueORF®
METRNL (tGFP-tagged) - Human meteorin, glial cell differentiation regulator-like (METRNL)
ORF Plasmid: DDK
"NM_001004431" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210299 representing NM_001004431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCGCGGCGCGGGCGGCCTGGGGGCGCGCGGGGCAGCCGTGGCCGCGACCCCCCGCCCCGGGCC CGCCCCCGCCGCCGCTCCCGCTGCTGCTCCTGCTCCTGGCCGGGCTGCTGGGCGGCGCGGGCGCGCAGTA CTCCAGCGACCGGTGCAGCTGGAAGGGGAGCGGGCTGACGCACGAGGCACACAGGAAGGAGGTGGAGCAG GTGTATCTGCGCTGTGCGGCGGGTGCCGTGGAGTGGATGTACCCAACAGGTGCTCTCATCGTTAACCTGC GGCCCAACACCTTCTCGCCTGCCCGGCACCTGACCGTGTGCATCAGGTCCTTCACGGACTCCTCGGGGGC CAATATTTATTTGGAAAAAACTGGAGAACTGAGACTGCTGGTACCGGACGGGGACGGCAGGCCCGGCCGG GTGCAGTGTTTTGGCCTGGAGCAGGGCGGCCTGTTCGTGGAGGCCACGCCGCAGCAGGATATCGGCCGGA GGACCACAGGCTTCCAGTACGAGCTGGTTAGGAGGCACAGGGCGTCGGACCTGCACGAGCTGTCTGCGCC GTGCCGTCCCTGCAGTGACACCGAGGTGCTCCTAGCCGTCTGCACCAGCGACTTCGCCGTTCGAGGCTCC ATCCAGCAAGTTACCCACGAGCCTGAGCGGCAGGACTCAGCCATCCACCTGCGCGTGAGCAGACTCTATC GGCAGAAAAGCAGGGTCTTCGAGCCGGTGCCCGAGGGTGACGGCCACTGGCAGGGGCGCGTCAGGACGCT GCTGGAGTGTGGCGTGCGGCCGGGGCATGGCGACTTCCTCTTCACTGGCCACATGCACTTCGGGGAGGCG CGGCTCGGCTGTGCCCCACGCTTCAAGGACTTCCAGAGGATGTACAGGGATGCCCAGGAGAGGGGGCTGA ACCCTTGTGAGGTTGGCACGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210299 representing NM_001004431
Red=Cloning site Green=Tags(s) MRGAARAAWGRAGQPWPRPPAPGPPPPPLPLLLLLLAGLLGGAGAQYSSDRCSWKGSGLTHEAHRKEVEQ VYLRCAAGAVEWMYPTGALIVNLRPNTFSPARHLTVCIRSFTDSSGANIYLEKTGELRLLVPDGDGRPGR VQCFGLEQGGLFVEATPQQDIGRRTTGFQYELVRRHRASDLHELSAPCRPCSDTEVLLAVCTSDFAVRGS IQQVTHEPERQDSAIHLRVSRLYRQKSRVFEPVPEGDGHWQGRVRTLLECGVRPGHGDFLFTGHMHFGEA RLGCAPRFKDFQRMYRDAQERGLNPCEVGTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001004431 |
| ORF Size | 933 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001004431.1, NP_001004431.1 |
| RefSeq Size | 1348 bp |
| RefSeq ORF | 936 bp |
| Locus ID | 284207 |
| UniProt ID | Q641Q3 |
| Gene Summary | Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210299 | METRNL (Myc-DDK-tagged)-Human meteorin, glial cell differentiation regulator-like (METRNL) |
CNY 3,600.00 |
|
| RC210299L3 | Lenti-ORF clone of METRNL (Myc-DDK-tagged)-Human meteorin, glial cell differentiation regulator-like (METRNL) |
CNY 5,890.00 |
|
| RC210299L4 | Lenti-ORF clone of METRNL (mGFP-tagged)-Human meteorin, glial cell differentiation regulator-like (METRNL) |
CNY 5,890.00 |
|
| SC106468 | METRNL (untagged)-Human meteorin, glial cell differentiation regulator-like (METRNL) |
CNY 3,600.00 |
