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PCSK7 (NM_004716) Human Tagged ORF Clone
CAT＃: RG203896
- TrueORF®
PCSK7 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 7 (PCSK7)
ORF Plasmid: DDK
"NM_004716" in other vectors (7)
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CNY 6,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | LPC; PC7; PC8; SPC7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203896 representing NM_004716
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGGGGAGGCAGAAAGTGCCACACTTGGATGCCCCCCTGGGCCTGCCCACCTGCCTCTGGCTGG AATTAGCCGGGCTCTTCTTACTGGTTCCCTGGGTCATGGGCCTGGCAGGGACAGGTGGGCCTGATGGCCA GGGCACAGGGGGGCCGAGCTGGGCTGTGCACCTGGAAAGCCTGGAAGGTGACGGGGAGGAAGAGACTCTG GAGCAGCAGGCGGATGCCTTGGCCCAGGCAGCAGGGCTGGTGAATGCTGGACGCATCGGAGAGCTTCAGG GGCACTACCTCTTTGTCCAGCCTGCTGGGCACAGGCCGGCCCTGGAGGTGGAGGCCATCCGGCAGCAGGT GGAGGCTGTGTTGGCTGGGCATGAAGCTGTGCGCTGGCACTCAGAGCAGAGGCTGCTAAGGCGGGCCAAG CGCAGCGTCCACTTCAACGACCCCAAGTACCCGCAGCAATGGCACCTGAATAACCGACGGAGCCCGGGCA GGGACATCAACGTGACGGGTGTGTGGGAACGCAATGTGACTGGGCGAGGGGTGACGGTGGTGGTAGTGGA TGACGGAGTGGAACACACCATCCAGGACATTGCACCCAACTATAGCCCTGAGGGTAGCTATGACCTCAAC TCTAATGACCCTGACCCCATGCCCCACCCGGATGTGGAGAATGGCAACCACCATGGCACGCGATGTGCAG GAGAGATCGCGGCTGTGCCCAACAACAGCTTCTGTGCCGTGGGCGTGGCCTACGGGAGCCGCATCGCAGG TATCCGGGTACTGGATGGACCTCTCACAGACAGCATGGAGGCAGTGGCGTTCAACAAGCACTATCAGATC AATGACATCTACAGCTGCAGCTGGGGACCAGATGACGATGGGAAGACAGTGGATGGCCCCCATCAGCTTG GAAAGGCTGCCTTACAACATGGGGTGATTGCTGGTCGCCAGGGCTTTGGGAGCATCTTTGTGGTAGCCAG TGGCAACGGAGGCCAACACAACGACAACTGCAACTACGATGGCTACGCCAACTCCATCTACACCGTCACC ATAGGAGCTGTGGATGAGGAGGGACGCATGCCTTTCTATGCAGAAGAATGTGCCTCCATGCTGGCAGTCA CCTTCAGTGGTGGGGACAAGATGCTTCGGAGCATTGTGACCACTGACTGGGACCTTCAGAAGGGCACTGG CTGCACTGAGGGCCACACAGGGACCTCAGCTGCAGCGCCTCTGGCAGCTGGCATGATAGCCTTAATGCTG CAGGTGCGGCCCTGCCTCACGTGGCGTGACGTCCAGCACATCATTGTCTTCACAGCCACCCGGTATGAGG ATCGCCGTGCAGAGTGGGTCACCAACGAGGCAGGCTTCAGCCATAGCCACCAGCACGGTTTCGGCCTCCT CAACGCCTGGAGGCTCGTGAATGCAGCCAAGATCTGGACATCTGTCCCTTACTTAGCATCCTACGTCAGT CCCGTGTTAAAAGAAAACAAGGCGATTCCGCAGTCCCCCCGTTCCCTGGAGGTCCTGTGGAATGTCAGCA GGATGGACCTGGAGATGTCAGGGCTGAAGACCCTGGAGCATGTGGCAGTGACAGTCTCCATCACTCACCC ACGGCGCGGCAGCTTGGAGCTGAAGCTGTTCTGCCCCAGTGGCATGATGTCCCTCATCGGCGCCCCCCGC AGCATGGACTCGGATCCCAACGGCTTCAATGACTGGACCTTCTCCACTGTGCGATGCTGGGGGGAGAGAG CCCGAGGGACCTACAGGCTTGTCATCAGGGATGTCGGGGATGAGTCATTCCAGGTCGGCATCCTCCGGCA ATGGCAGCTGACCCTATATGGCTCTGTGTGGAGTGCAGTAGACATCAGGGACAGACAAAGGCTGTTAGAG AGTGCCATGAGTGGAAAATACCTGCACGATGACTTCGCCCTGCCCTGCCCACCGGGGCTGAAAATTCCTG AGGAAGATGGTTACACCATCACCCCCAACACCCTCAAGACCCTGGTGCTGGTAGGCTGTTTCACCGTCTT CTGGACTGTTTACTACATGCTGGAAGTATATTTGAGCCAGAGGAATGTGGCTTCCAATCAAGTTTGTAGG AGTGGACCCTGCCACTGGCCCCATCGGAGCCGGAAAGCCAAGGAGGAAGGGACAGAGCTAGAATCAGTGC CACTTTGCAGCAGCAAGGATCCAGACGAAGTGGAAACAGAGAGCAGGGGCCCTCCCACCACCTCTGACCT CCTTGCCCCAGACCTGCTGGAGCAAGGGGACTGGAGCCTGTCCCAGAACAAGAGCGCCCTGGACTGCCCT CATCAGCACCTAGACGTACCGCACGGGAAGGAGGAGCAGATCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203896 representing NM_004716
Red=Cloning site Green=Tags(s) MPKGRQKVPHLDAPLGLPTCLWLELAGLFLLVPWVMGLAGTGGPDGQGTGGPSWAVHLESLEGDGEEETL EQQADALAQAAGLVNAGRIGELQGHYLFVQPAGHRPALEVEAIRQQVEAVLAGHEAVRWHSEQRLLRRAK RSVHFNDPKYPQQWHLNNRRSPGRDINVTGVWERNVTGRGVTVVVVDDGVEHTIQDIAPNYSPEGSYDLN SNDPDPMPHPDVENGNHHGTRCAGEIAAVPNNSFCAVGVAYGSRIAGIRVLDGPLTDSMEAVAFNKHYQI NDIYSCSWGPDDDGKTVDGPHQLGKAALQHGVIAGRQGFGSIFVVASGNGGQHNDNCNYDGYANSIYTVT IGAVDEEGRMPFYAEECASMLAVTFSGGDKMLRSIVTTDWDLQKGTGCTEGHTGTSAAAPLAAGMIALML QVRPCLTWRDVQHIIVFTATRYEDRRAEWVTNEAGFSHSHQHGFGLLNAWRLVNAAKIWTSVPYLASYVS PVLKENKAIPQSPRSLEVLWNVSRMDLEMSGLKTLEHVAVTVSITHPRRGSLELKLFCPSGMMSLIGAPR SMDSDPNGFNDWTFSTVRCWGERARGTYRLVIRDVGDESFQVGILRQWQLTLYGSVWSAVDIRDRQRLLE SAMSGKYLHDDFALPCPPGLKIPEEDGYTITPNTLKTLVLVGCFTVFWTVYYMLEVYLSQRNVASNQVCR SGPCHWPHRSRKAKEEGTELESVPLCSSKDPDEVETESRGPPTTSDLLAPDLLEQGDWSLSQNKSALDCP HQHLDVPHGKEEQIC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004716 |
| ORF Size | 2355 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004716.4 |
| RefSeq Size | 3497 bp |
| RefSeq ORF | 2358 bp |
| Locus ID | 9159 |
| UniProt ID | Q16549 |
| Domains | Peptidase_S8, P_proprotein |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Gene Summary | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It can process proalbumin and is thought to be responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene has been implicated in the transcriptional regulation of housekeeping genes and plays a role in the regulation of iron metabolism. A t(11;14)(q23;q32) chromosome translocation associated with B-cell lymphoma occurs between this gene and its inverted counterpart. [provided by RefSeq, Feb 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203896 | PCSK7 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 7 (PCSK7) |
CNY 5,752.00 |
|
| RC203896L1 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 7 (PCSK7), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC203896L2 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 7 (PCSK7), mGFP tagged |
CNY 7,700.00 |
|
| RC203896L3 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 7 (PCSK7), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC203896L4 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 7 (PCSK7), mGFP tagged |
CNY 7,700.00 |
|
| SC117224 | PCSK7 (untagged)-Human proprotein convertase subtilisin/kexin type 7 (PCSK7) |
CNY 2,950.00 |
|
| SC323814 | PCSK7 (untagged)-Human proprotein convertase subtilisin/kexin type 7 (PCSK7) |
CNY 2,950.00 |
