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GBA2 (NM_020944) Human Tagged ORF Clone
CAT＃: RG203706
- TrueORF®
GBA2 (tGFP-tagged) - Human glucosidase, beta (bile acid) 2 (GBA2)
ORF Plasmid: DDK
"NM_020944" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AD035; NLGase; SPG46 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203706 representing NM_020944
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACCCAGGATCCAGGGAACATGGGAACCGGCGTCCCAGCCTCGGAGCAGATAAGCTGTGCCAAAG AGGATCCACAAGTTTATTGCCCTGAAGAGACTGGCGGCACCAAGGATGTGCAGGTTACAGACTGTAAGAG TCCCGAAGACAGCCGACCCCCAAAAGAGACGGACTGCTGCAATCCGGAGGACTCTGGGCAGCTGATGGTT TCCTATGAGGGTAAAGCTATGGGCTACCAGGTGCCTCCCTTTGGCTGGCGCATCTGTCTGGCTCATGAGT TTACAGAGAAGAGGAAACCCTTTCAAGCTAACAACGTCTCCCTAAGCAACATGATAAAGCATATAGGCAT GGGCTTGAGGTACCTGCAGTGGTGGTACCGGAAGACCCATGTGGAAAAGAAGACACCTTTCATCGACATG ATCAATTCTGTACCCCTAAGACAGATTTATGGTTGTCCCTTGGGTGGCATCGGGGGAGGCACTATTACCC GTGGCTGGAGAGGCCAGTTCTGTCGTTGGCAGCTTAACCCTGGAATGTATCAGCACCGGACAGTCATCGC TGACCAATTCACAGTGTGCCTGCGTCGGGAAGGGCAGACTGTGTACCAGCAAGTCCTGTCCCTGGAGCGC CCAAGTGTCCTCCGCAGCTGGAACTGGGGCCTGTGTGGGTACTTTGCTTTCTACCATGCCCTCTATCCCC GAGCCTGGACTGTCTATCAGCTTCCTGGCCAGAATGTCACCCTCACCTGCCGTCAGATCACACCCATCTT GCCCCATGACTACCAGGACAGCAGCCTGCCTGTAGGAGTCTTTGTGTGGGATGTGGAAAATGAAGGGGAC GAAGCTCTAGATGTGTCCATCATGTTCTCCATGCGGAATGGACTGGGTGGTGGAGACGATGCCCCAGGGG GTTTGTGGAATGAGCCCTTCTGTCTGGAGCGTAGCGGGGAAACTGTCCGGGGGCTGCTCCTGCATCATCC AACCCTTCCAAACCCCTACACGATGGCTGTGGCTGCACGAGTCACGGCAGCTACCACGGTAACCCACATC ACAGCCTTTGACCCTGACAGCACGGGGCAGCAGGTGTGGCAGGATCTACTTCAGGATGGACAGCTGGACT CTCCCACTGGCCAAAGCACCCCTACGCAGAAAGGAGTAGGCATTGCTGGAGCTGTGTGTGTTTCCAGCAA GTTGCGACCTCGAGGCCAGTGCCGCCTGGAGTTTTCACTGGCTTGGGACATGCCCAGGATCATGTTTGGA GCTAAAGGCCAAGTCCACTACAGGCGGTATACAAGGTTCTTTGGCCAGGATGGAGATGCAGCACCTGCCC TCAGCCACTATGCACTGTGCCGATACGCAGAGTGGGAAGAGAGGATCTCAGCTTGGCAGAGCCCGGTATT GGATGACAGATCACTGCCTGCCTGGTACAAATCTGCGCTGTTCAATGAACTATACTTCCTGGCTGATGGA GGCACAGTGTGGCTGGAAGTTCTTGAGGACTCCCTACCAGAGGAGCTGGGCAGAAACATGTGTCACCTCC GCCCCACCCTACGGGACTACGGTCGATTTGGCTACCTTGAGGGCCAGGAGTACCGCATGTACAACACATA TGATGTCCACTTTTATGCTTCCTTTGCCCTCATCATGCTCTGGCCCAAACTTGAGCTCAGCCTACAGTAT GACATGGCTCTGGCCACTCTCAGGGAGGACCTGACACGGCGACGGTACCTGATGAGTGGGGTGATGGCAC CTGTGAAAAGGAGGAACGTCATCCCCCATGATATTGGGGACCCAGATGATGAACCATGGCTCCGCGTCAA TGCATATTTAATCCATGATACTGCTGATTGGAAGGACCTGAACCTGAAGTTTGTGCTGCAGGTTTATCGG GACTATTACCTCACGGGTGATCAAAACTTCCTGAAGGACATGTGGCCTGTGTGTCTAGCTGTGATGGAAT CTGAAATGAAGTTTGACAAGGACCATGATGGACTCATTGAAAATGGAGGCTATGCAGACCAGACCTATGA TGGATGGGTGACCACAGGCCCCAGTGCTTACTGTGGAGGGCTGTGGCTGGCAGCTGTGGCTGTGATGGTC CAGATGGCTGCTCTGTGTGGGGCACAGGACATCCAGGATAAGTTTTCTTCTATCCTCAGCCGGGGCCAAG AAGCCTATGAGAGACTGCTGTGGAATGGCCGCTATTACAACTATGACAGCAGCTCTCGGCCTCAGTCTCG TAGTGTTATGTCTGACCAGTGTGCTGGACAGTGGTTCCTGAAGGCCTGTGGCCTAGGAGAAGGAGACACT GAGGTGTTTCCTACCCAACATGTGGTCCGTGCTCTCCAAACTATCTTTGAGCTGAACGTCCAGGCCTTTG CAGGAGGGGCCATGGGGGCTGTGAATGGGATGCAGCCCCATGGTGTCCCTGATAAATCCAGTGTGCAGTC TGATGAAGTCTGGGTGGGTGTGGTCTACGGGCTGGCAGCTACCATGATCCAAGAGGGCCTGACTTGGGAG GGCTTCCAGACAGCTGAAGGCTGCTACCGTACCGTGTGGGAGCGCCTGGGTCTGGCCTTCCAGACCCCAG AGGCATACTGCCAGCAGCGAGTGTTCCGCTCACTGGCCTACATGCGGCCACTGAGCATATGGGCCATGCA GCTAGCCCTGCAACAGCAGCAGCACAAAAAGGCCTCCTGGCCAAAAGTCAAACAGGGCACAGGACTAAGG ACAGGGCCTATGTTTGGACCAAAGGAAGCCATGGCAAACCTGAGCCCAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203706 representing NM_020944
Red=Cloning site Green=Tags(s) MGTQDPGNMGTGVPASEQISCAKEDPQVYCPEETGGTKDVQVTDCKSPEDSRPPKETDCCNPEDSGQLMV SYEGKAMGYQVPPFGWRICLAHEFTEKRKPFQANNVSLSNMIKHIGMGLRYLQWWYRKTHVEKKTPFIDM INSVPLRQIYGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHRTVIADQFTVCLRREGQTVYQQVLSLER PSVLRSWNWGLCGYFAFYHALYPRAWTVYQLPGQNVTLTCRQITPILPHDYQDSSLPVGVFVWDVENEGD EALDVSIMFSMRNGLGGGDDAPGGLWNEPFCLERSGETVRGLLLHHPTLPNPYTMAVAARVTAATTVTHI TAFDPDSTGQQVWQDLLQDGQLDSPTGQSTPTQKGVGIAGAVCVSSKLRPRGQCRLEFSLAWDMPRIMFG AKGQVHYRRYTRFFGQDGDAAPALSHYALCRYAEWEERISAWQSPVLDDRSLPAWYKSALFNELYFLADG GTVWLEVLEDSLPEELGRNMCHLRPTLRDYGRFGYLEGQEYRMYNTYDVHFYASFALIMLWPKLELSLQY DMALATLREDLTRRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYR DYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMV QMAALCGAQDIQDKFSSILSRGQEAYERLLWNGRYYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDT EVFPTQHVVRALQTIFELNVQAFAGGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWE GFQTAEGCYRTVWERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQQHKKASWPKVKQGTGLR TGPMFGPKEAMANLSPE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020944 |
| ORF Size | 2781 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020944.3 |
| RefSeq Size | 3639 bp |
| RefSeq ORF | 2784 bp |
| Locus ID | 57704 |
| UniProt ID | Q9HCG7 |
| Domains | DUF608 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides as endogenous compounds. Studies to determine subcellular localization of this protein in the liver indicated that the enzyme was mainly enriched in the microsomal fraction where it appeared to be confined to the endoplasmic reticulum. This putative transmembrane protein is thought to play a role in carbohydrate transport and metabolism. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203706 | GBA2 (Myc-DDK-tagged)-Human glucosidase, beta (bile acid) 2 (GBA2) |
CNY 3,990.00 |
|
| RC203706L1 | Lenti ORF clone of Human glucosidase, beta (bile acid) 2 (GBA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203706L2 | Lenti ORF clone of Human glucosidase, beta (bile acid) 2 (GBA2), mGFP tagged |
CNY 5,890.00 |
|
| RC203706L3 | Lenti ORF clone of Human glucosidase, beta (bile acid) 2 (GBA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203706L4 | Lenti ORF clone of Human glucosidase, beta (bile acid) 2 (GBA2), mGFP tagged |
CNY 5,890.00 |
|
| SC323811 | GBA2 (untagged)-Human glucosidase, beta (bile acid) 2 (GBA2) |
CNY 6,800.00 |
