TIMP1 (NM_003254) Human Tagged ORF Clone
CAT#: RG201548
- TrueORF®
TIMP1 (tGFP-tagged) - Human TIMP metallopeptidase inhibitor 1 (TIMP1)
ORF Plasmid: DDK
"NM_003254" in other vectors (6)
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CNY 5,200.00
Cited in 4 publications. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CLGI; EPA; EPO; HCI; TIMP; TIMP-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201548 representing NM_003254
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCTTTGAGCCCCTGGCTTCTGGCATCCTGTTGTTGCTGTGGCTGATAGCCCCCAGCAGGGCCT GCACCTGTGTCCCACCCCACCCACAGACGGCCTTCTGCAATTCCGACCTCGTCATCAGGGCCAAGTTCGT GGGGACACCAGAAGTCAACCAGACCACCTTATACCAGCGTTATGAGATCAAGATGACCAAGATGTATAAA GGGTTCCAAGCCTTAGGGGATGCCGCTGACATCCGGTTCGTCTACACCCCCGCCATGGAGAGTGTCTGCG GATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTGGAAAACTGCAGGATGGACTCTT GCACATCACTACCTGCAGTTTCGTGGCTCCCTGGAACAGCCTGAGCTTAGCTCAGCGCCGGGGCTTCACC AAGACCTACACTGTTGGCTGTGAGGAATGCACAGTGTTTCCCTGTTTATCCATCCCCTGCAAACTGCAGA GTGGCACTCATTGCTTGTGGACGGACCAGCTCCTCCAAGGCTCTGAAAAGGGCTTCCAGTCCCGTCACCT TGCCTGCCTGCCTCGGGAGCCAGGGCTGTGCACCTGGCAGTCCCTGCGGTCCCAGATAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201548 representing NM_003254
Red=Cloning site Green=Tags(s) MAPFEPLASGILLLLWLIAPSRACTCVPPHPQTAFCNSDLVIRAKFVGTPEVNQTTLYQRYEIKMTKMYK GFQALGDAADIRFVYTPAMESVCGYFHRSHNRSEEFLIAGKLQDGLLHITTCSFVAPWNSLSLAQRRGFT KTYTVGCEECTVFPCLSIPCKLQSGTHCLWTDQLLQGSEKGFQSRHLACLPREPGLCTWQSLRSQIA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003254 |
ORF Size | 621 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003254.1 |
RefSeq Size | 931 bp |
RefSeq ORF | 624 bp |
Locus ID | 7076 |
UniProt ID | P01033 |
Domains | NTR |
Protein Families | Druggable Genome, Secreted Protein |
Gene Summary | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
---|
Substrate Stiffness-Driven Membrane Tension Modulates Vesicular Trafficking via Caveolin-1
,null,
ACS Nano
,PubMed ID 35255206
[TIMP1]
|
Angiogenic factor-driven inflammation promotes extravasation of human proangiogenic monocytes to tumours
,null,
Nature Communications
,PubMed ID 29367702
[TIMP1]
|
Identification of a cytogenetic and molecular subgroup of acute myeloid leukemias showing sensitivity to L-Asparaginase
,Bertuccio, SN;Serravalle, S;Astolfi, A;Lonetti, A;Indio, V;Leszl, A;Pession, A;Melchionda, F;,
Oncotarget
,PubMed ID 29299118
[TIMP1]
|
Down-regulation of TIMP-1 inhibits cell migration, invasion, and metastatic colonization in lung adenocarcinoma
,Chang, YH;Chiu, YJ;Cheng, HC;Liu, FJ;Lai, WW;Chang, HJ;Liao, PC;,
Tumour Biol.
,PubMed ID 25578494
[TIMP1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201548 | TIMP1 (Myc-DDK-tagged)-Human TIMP metallopeptidase inhibitor 1 (TIMP1) |
CNY 3,600.00 |
|
RC201548L1 | Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC201548L2 | Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), mGFP tagged |
CNY 5,890.00 |
|
RC201548L3 | Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201548L4 | Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), mGFP tagged |
CNY 6,000.00 |
|
SC118082 | TIMP1 (untagged)-Human TIMP metallopeptidase inhibitor 1 (TIMP1) |
CNY 2,400.00 |