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RNF8 (NM_003958) Human Tagged ORF Clone
CAT＃: RG201402
- TrueORF®
RNF8 (tGFP-tagged) - Human ring finger protein 8 (RNF8), transcript variant 1
ORF Plasmid: DDK
"NM_003958" in other vectors (6)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | hRNF8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201402 representing NM_003958
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGCCCGGCTTCTTCGTCACAGGAGACCGCGCCGGTGGCCGGAGCTGGTGCCTGCGGCGGGTGG GGATGAGCGCCGGGTGGCTGCTGCTGGAAGATGGGTGCGAGGTGACTGTAGGACGAGGATTTGGTGTCAC ATACCAACTGGTATCAAAAATCTGCCCCCTGATGATTTCTCGAAACCACTGTGTTTTGAAGCAGAATCCT GAGGGCCAATGGACAATTATGGACAACAAGAGTCTAAATGGTGTTTGGCTGAACAGAGCGCGTCTGGAAC CTTTAAGGGTCTATTCCATTCATCAGGGAGACTACATCCAACTTGGAGTGCCTCTGGAAAATAAGGAGAA TGCGGAGTATGAATATGAAGTTACTGAAGAAGACTGGGAGACAATATATCCTTGTCTTTCCCCAAAGAAT GACCAAATGATAGAAAAAAATAAGGAATTGAGAACTAAAAGGAAATTCAGTTTGGATGAATTAGCAGGTC CTGGAGCTGAAGGCCCCTCAAATTTGAAATCCAAAATAAATAAAGTGTCTTGTGAATCTGGTCAGCCAGT GAAATCACAGGGGAAAGGTGAAGTGGCCAGTACACCCTCTGACAATTTGGATCCTAAGTTGACTGCCCTT GAGCCAAGTAAGACCACAGGGGCTCCCATTTACCCTGGCTTCCCCAAAGTCACAGAGGTTCATCATGAGC AGAAAGCCTCAAACTCTTCAGCATCTCAGAGAAGCTTACAGATGTTTAAGGTGACCATGTCCAGGATTCT GAGGCTCAAAATACAGATGCAGGAAAAACATGAAGCCGTTATGAATGTGAAAAAGCAGACCCAAAAGGGG AACTCAAAGAAAGTTGTGCAAATGGAGCAGGAACTTCAGGACTTACAGTCCCAGCTGTGTGCAGAGCAGG CTCAGCAGCAGGCAAGAGTGGAGCAACTAGAGAAGACTTTCCAGGAAGAGGAACAGCATCTTCAGGGTTT GGAGATAGCCCAAGGAGAAAAGGACCTGAAGCAACAGCTGGCCCAGGCTCTGCAGGAGCATTGGGCTCTA ATGGAAGAGCTAAATCGCAGCAAGAAGGACTTTGAAGCAATCATTCAAGCCAAGAACAAAGAATTAGAGC AGACCAAGGAAGAGAAGGAGAAGATGCAAGCACAGAAGGAAGAAGTTCTTAGCCACATGAATGATGTGCT AGAGAATGAGCTCCAATGTATTATTTGTTCAGAATACTTCATTGAGGCTGTCACCTTGAACTGTGCCCAC AGTTTCTGCTCCTACTGTATCAATGAATGGATGAAGCGGAAGATAGAATGCCCCATTTGTCGGAAGGACA TTAAGTCCAAAACATACTCTTTGGTTCTGGACAATTGCATTAATAAGATGGTAAATAATCTGAGCTCAGA AGTGAAAGAACGACGAATTGTTCTCATTAGGGAACGAAAAGCAAAGAGATTGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201402 representing NM_003958
Red=Cloning site Green=Tags(s) MGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQLVSKICPLMISRNHCVLKQNP EGQWTIMDNKSLNGVWLNRARLEPLRVYSIHQGDYIQLGVPLENKENAEYEYEVTEEDWETIYPCLSPKN DQMIEKNKELRTKRKFSLDELAGPGAEGPSNLKSKINKVSCESGQPVKSQGKGEVASTPSDNLDPKLTAL EPSKTTGAPIYPGFPKVTEVHHEQKASNSSASQRSLQMFKVTMSRILRLKIQMQEKHEAVMNVKKQTQKG NSKKVVQMEQELQDLQSQLCAEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEKDLKQQLAQALQEHWAL MEELNRSKKDFEAIIQAKNKELEQTKEEKEKMQAQKEEVLSHMNDVLENELQCIICSEYFIEAVTLNCAH SFCSYCINEWMKRKIECPICRKDIKSKTYSLVLDNCINKMVNNLSSEVKERRIVLIRERKAKRLF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003958 |
| ORF Size | 1455 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003958.2, NP_003949.1 |
| RefSeq Size | 5600 bp |
| RefSeq ORF | 1458 bp |
| Locus ID | 9025 |
| UniProt ID | O76064 |
| Domains | FHA, RING |
| Gene Summary | The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201402 | RNF8 (Myc-DDK-tagged)-Human ring finger protein 8 (RNF8), transcript variant 1 |
CNY 5,488.00 |
|
| RC201402L1 | Lenti ORF clone of Human ring finger protein 8 (RNF8), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201402L2 | Lenti ORF clone of Human ring finger protein 8 (RNF8), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201402L3 | Lenti ORF clone of Human ring finger protein 8 (RNF8), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201402L4 | Lenti ORF clone of Human ring finger protein 8 (RNF8), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC110018 | RNF8 (untagged)-Human ring finger protein 8 (RNF8), transcript variant 1 |
CNY 5,488.00 |
