NUP153 (NM_001278210) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HNUP153; N153
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240142 representing NM_001278210 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGGAGCCGGAGGAGTCGGAGGGGGCGGTGGCGGCAAGATCCGGACGCGGCGTTGCCACCAGG GGCCAATTAAGCCTTACCAGCAGGGGCGACAACAGCATCAGGGCATTCTTAGCAGGGTTACAGAATCTGT TAAGAATATTGTGCCAGGGTGGCTACAAAGATACTTCAACAAGAATGAAGATGTATGCAGCTGTTCAACA GACACAAGCGAGGTTCCACGCTGGCCAGAAAATAAAGAGGACCATCTGGTATATGCCGATGAGGAGAGCT CTAATATTACTGATGGGAGAATCACACCTGAGCCAGCAGTCAGTAATACAGAAGAACCTTCAACAACTAG TACTGCTTCAAATTATCCAGATGTGTTAACAAGGCCTTCTCTTCATCGGAGCCATCTGAATTTTTCCATG TTGGAATCCCCTGCATTACACTGTCAGCCATCTACATCCTCGGCATTCCCAATTGGCAGTTCGGGATTTT CCCTTGTAAAGGAAATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACCAGTGGTTT TTCTTCAAGAGCTTCTGATAAAGATATAACTGTTTCAAAGAACACTTCATTGCCACCTCTGTGGTCCCCA GAAGCTGAACGTTCTCACTCACTCTCACAGCACACTGCCACCAGCTCAAAAAAACCAGCATTCAACTTGT CTGCCTTTGGAACACTTTCCCCTTCACTTGGGAATTCTTCAATCCTTAAAACCAGTCAGCTTGGAGATTC TCCTTTTTATCCTGGAAAAACAACATACGGTGGGGCAGCAGCTGCTGTAAGACAGTCTAAACTACGAAAT ACACCTTATCAGGCACCAGTTAGAAGACAAATGAAAGCTAAGCAACTCAGTGCACAATCTTACGGTGTGA CCAGTTCAACAGCTCGGCGAATATTGCAGTCTTTAGAGAAGATGTCAAGCCCTTTAGCGGATGCAAAAAG AATTCCATCCATTGTTTCTTCTCCTCTGAATTCTCCTCTTGATAGGAGTGGGATAGATATCACAGATTTT CAGGCCAAAAGAGAAAAGGTGGATTCTCAATATCCTCCTGTTCAGAGACTTATGACCCCAAAGCCAGTTT CCATAGCAACAAATCGAAGTGTTTATTTTAAACCATCTCTGACTCCTTCTGGTGAATTCAGGAAGACTAA TCAAAGAATAGATAACAAGTGCAGTACTGGATATGAAAAAAATATGACACCCGGACAAAATAGAGAACAA CGAGAAAGTGGCTTTTCATATCCAAATTTCAGTTTGCCTGCAGCCAATGGTTTATCTTCTGGAGTAGGTG GTGGAGGTGGCAAGATGAGACGAGAAAGAACACGCTTTGTTGCTTCTAAACCTCTGGAGGAGGAGGAAAT GGAAGTTCCAGTATTACCGAAAATCTCTCTACCGATCACCAGTTCTTCACTGCCTACCTTTAATTTTAGT TCCCCTGAGATCACAACTTCCTCTCCATCACCCATCAATTCGTCTCAAGCATTAACAAACAAGGTACAAA TGACCTCTCCGAGCAGCACTGGCAGTCCCATGTTTAAATTTTCATCTCCAATCGTAAAATCTACTGAGGC AAATGTACTACCTCCATCATCTATTGGATTTACATTTAGTGTGCCTGTTGCAAAAACAGCAGAACTTTCT GGTTCTAGTAGTACTTTAGAACCAATTATAAGTAGTTCAGGTTTCGCATCGCCGAAGATAGATTCTGTTG CTGCTCAGCCCACCGCAACAAGCCCAGTAGTTTATACAAGACCAGCAATAAGTAGCTTTTCTTCTAGTGG AATTGGGTTTGGGGAGAGTTTAAAAGCTGGGTCATCATGGCAGTGTGATACATGTCTACTCCAGAACAAA GTTACAGACAACAAATGCATAGCCTGTCAAGCAGCAAAATTGTCACCCAGAGATACTGCTAAACAGACTG GAATTGAAACACCAAATAAAAGTGGCAAAACAACTCTTTCTGCATCAGGGACAGGCTTTGGAGACAAATT TAAACCAGTGATAGGCACTTGGGATTGTGATACCTGTTTAGTGCAAAATAAACCTGAAGCAATAAAATGT GTAGCCTGTGAAACACCGAAACCTGGAACTTGTGTGAAGCGAGCCCTTACATTGACAGTGGTTTCGGAAA GTGCTGAGACTATGACTGCTTCATCTTCCAGCTGCACTGTAACCACTGGTACCTTAGGATTTGGAGATAA ATTCAAAAGGCCCATTGGATCTTGGGAGTGTTCAGTATGCTGTGTTTCTAATAATGCAGAAGACAATAAG TGTGTGTCCTGTATGTCTGAGAAACCAGGAAGTTCAGTACCTGCTTCAAGTAGCAGCACTGTACCTGTCT CTCTGCCTTCTGGAGGCTCTCTAGGATTGGAAAAGTTCAAGAAACCCGAGGGAAGCTGGGACTGTGAATT GTGCCTAGTGCAGAATAAGGCAGACTCTACCAAATGTTTGGCATGTGAAAGTGCAAAGCCAGGCACAAAA TCTGGGTTTAAAGGCTTTGACACATCTTCCTCATCTTCGAACTCAGCAGCCTCCTCATCCTTCAAATTTG GTGTCTCATCATCCTCTTCTGGGCCTTCTCAGACTTTAACAAGCACTGGAAATTTTAAATTTGGAGATCA GGGAGGATTCAAAATAGGTGTGTCATCCGATTCTGGGTCTATAAACCCCATGAGTGAAGGCTTTAAATTT TCTAAACCAATAGGAGATTTTAAATTTGGAGTTTCATCTGAATCTAAGCCCGAAGAAGTTAAAAAAGATA GTAAGAATGATAATTTTAAGTTTGGACTTTCTTCTGGTTTAAGCAACCCAGTTTCTTTAACTCCATTTCA ATTTGGGGTATCTAATCTTGGACAGGAAGAAAAGAAAGAGGAACTGCCCAAATCTTCCTCTGCAGGTTTT AGCTTTGGTACAGGTGTTATTAACTCCACCCCTGCTCCTGCTAACACCATAGTGACCTCTGAGAACAAGA GCAGCTTCAACCTTGGAACCATAGAAACCAAGAGTGCTTCAGTGGCTCCTTTCACATGTAAGACATCAGA AGCTAAAAAAGAAGAAATGCCTGCCACCAAAGGAGGATTCTCTTTTGGCAACGTGGAGCCTGCCTCTCTG CCATCTGCCTCAGTGTTTGTTTTGGGAAGGACAGAAGAGAAACAGCAAGAGCCTGTCACTTCTACTTCCC TAGTTTTTGGGAAGAAAGCTGACAATGAAGAGCCAAAGTGTCAACCAGTGTTTTCCTTTGGGAATTCAGA GCAAACCAAAGATGAGAATTCTTCAAAGTCCACATTTAGTTTTAGTATGACAAAACCATCTGAGAAGGAA TCTGAACAGCCAGCAAAAGCCACTTTTGCCTTTGGAGCTCAAACTAGTACTACAGCTGATCAAGGTGCAG CAAAGCCAGTTTTTAGTTTCTTGAACAACAGTTCCTCTAGTTCAAGTACACCAGCCACTTCTGCTGGTGG TGGCATATTTGGTAGTTCCACCTCTTCCTCCAATCCACCTGTGGCTACCTTTGTGTTTGGACAGTCCAGC AATCCTGTGAGCAGCTCTGCCTTTGGTAACACTGCTGAATCCAGCACCTCTCAGTCTTTGCTATTTTCTC AAGATAGCAAACTAGCAACCACATCCAGCACAGGTACAGCTGTCACCCCATTTGTCTTTGGTCCAGGAGC CAGCAGTAATAATACTACCACCTCTGGTTTCGGCTTTGGAGCCACAACCACATCTAGCTCTGCAGGATCC TCCTTTGTATTTGGAACTGGACCCTCAGCACCATCTGCCAGTCCAGCATTTGGTGCTAACCAGACCCCAA CATTTGGACAAAGTCAAGGTGCCAGCCAGCCCAATCCCCCAGGCTTTGGATCTATATCATCTTCCACAGC ATTATTTCCCACTGGTTCTCAGCCTGCACCACCTACTTTTGGGACAGTGTCAAGCAGTAGCCAGCCCCCT GTGTTTGGACAGCAACCTAGTCAGTCTGCATTTGGCTCTGGAACAACTCCTAATTCTAGTTCGGCTTTCC AGTTTGGCAGCAGCACTACAAATTTCAACTTCACAAACAACAGTCCATCAGGAGTGTTCACATTTGGTGC AAATTCTAGCACACCTGCAGCCTCAGCCCAGCCTTCAGGCTCGGGGGGCTTTCCATTTAACCAGTCTCCA GCAGCATTTACAGTGGGGTCAAATGGGAAAAATGTGTTCTCTTCTTCTGGAACTTCATTCTCTGGTCGCA AGATAAAGACTGCTGTTAGACGCAGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240142 representing NM_001278210 Red=Cloning site Green=Tags(s) MASGAGGVGGGGGGKIRTRRCHQGPIKPYQQGRQQHQGILSRVTESVKNIVPGWLQRYFNKNEDVCSCST DTSEVPRWPENKEDHLVYADEESSNITDGRITPEPAVSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFSM LESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSLPPLWSP EAERSHSLSQHTATSSKKPAFNLSAFGTLSPSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQSKLRN TPYQAPVRRQMKAKQLSAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSIVSSPLNSPLDRSGIDITDF QAKREKVDSQYPPVQRLMTPKPVSIATNRSVYFKPSLTPSGEFRKTNQRIDNKCSTGYEKNMTPGQNREQ RESGFSYPNFSLPAANGLSSGVGGGGGKMRRERTRFVASKPLEEEEMEVPVLPKISLPITSSSLPTFNFS SPEITTSSPSPINSSQALTNKVQMTSPSSTGSPMFKFSSPIVKSTEANVLPPSSIGFTFSVPVAKTAELS GSSSTLEPIISSSGFASPKIDSVAAQPTATSPVVYTRPAISSFSSSGIGFGESLKAGSSWQCDTCLLQNK VTDNKCIACQAAKLSPRDTAKQTGIETPNKSGKTTLSASGTGFGDKFKPVIGTWDCDTCLVQNKPEAIKC VACETPKPGTCVKRALTLTVVSESAETMTASSSSCTVTTGTLGFGDKFKRPIGSWECSVCCVSNNAEDNK CVSCMSEKPGSSVPASSSSTVPVSLPSGGSLGLEKFKKPEGSWDCELCLVQNKADSTKCLACESAKPGTK SGFKGFDTSSSSSNSAASSSFKFGVSSSSSGPSQTLTSTGNFKFGDQGGFKIGVSSDSGSINPMSEGFKF SKPIGDFKFGVSSESKPEEVKKDSKNDNFKFGLSSGLSNPVSLTPFQFGVSNLGQEEKKEELPKSSSAGF SFGTGVINSTPAPANTIVTSENKSSFNLGTIETKSASVAPFTCKTSEAKKEEMPATKGGFSFGNVEPASL PSASVFVLGRTEEKQQEPVTSTSLVFGKKADNEEPKCQPVFSFGNSEQTKDENSSKSTFSFSMTKPSEKE SEQPAKATFAFGAQTSTTADQGAAKPVFSFLNNSSSSSSTPATSAGGGIFGSSTSSSNPPVATFVFGQSS NPVSSSAFGNTAESSTSQSLLFSQDSKLATTSSTGTAVTPFVFGPGASSNNTTTSGFGFGATTTSSSAGS SFVFGTGPSAPSASPAFGANQTPTFGQSQGASQPNPPGFGSISSSTALFPTGSQPAPPTFGTVSSSSQPP VFGQQPSQSAFGSGTTPNSSSAFQFGSSTTNFNFTNNSPSGVFTFGANSSTPAASAQPSGSGGFPFNQSP AAFTVGSNGKNVFSSSGTSFSGRKIKTAVRRRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278210
ORF Size	4299 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278210.1, NP_001265139.1
RefSeq Size	5811 bp
RefSeq ORF	4302 bp
Locus ID	9972
UniProt ID	P49790
Protein Families	Druggable Genome, Stem cell - Pluripotency
MW	149.8 kDa
Gene Summary	Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

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