RICTOR (NM_001285440) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AVO3; hAVO3; PIA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240129 representing NM_001285440 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAATCATAGCAACATTCCGATCATGGGCAGGTATTATTAATTTATGTAAACCTGGAAATTCTGGGA TCCAGTCTCTAATAGGAGTACTTTGCATACCAAATATGGAAATAAGGCGAGGTCTACTTGAAGTGCTTTA TGATATATTTCGTCTTCCTCTACCTGTTGTGACTGAGGAGTTCATAGAAGCACTACTCAGTGTAGATCCA GGGAGGTTCCAAGACAGTTGGAGGCTTTCAGATGGCTTTGTGGCAGCTGAGGCAAAAACTATTCTTCCTC ATCGTGCCAGATCCAGGCCAGACCTCATGGATAATTATTTGGCACTGATACTCTCTGCATTTATTCGTAA TGGACTTTTAGAGGGTCTAGTTGAAGTGATAACAAACAGTGATGATCATATCTCAGTTAGAGCTACCATC CTTTTAGGAGAGCTTTTACATATGGCAAACACAATTCTTCCTCATTCACATAGCCATCATTTACACTGCT TGCCAACCCTAATGAATATGGCTGCATCCTTTGATATCCCCAAGGAAAAGAGACTGCGAGCCAGTGCAGC CTTGAACTGTTTAAAACGCTTCCATGAAATGAAGAAACGAGGACCTAAGCCTTATAGTCTTCATTTAGAC CACATTATTCAGAAAGCAATTGCAACACACCAGAAACGGGATCAGTATCTCCGAGTTCAGAAAGATATAT TTATCCTTAAGGATACAGAGGAAGCTCTTTTAATTAACCTTAGAGATAGCCAAGTCCTTCAACATAAAGA GAATCTTGAATGGAATTGGAATCTTATAGGGACCATTCTTAAGTGGCCAAATGTAAATCTAAGAAACTAT AAAGATGAACAGTTACACAGGTTTGTACGAAGACTACTTTATTTTTACAAGCCCAGCAGTAAATTATATG CCAACCTGGATCTGGATTTTGCCAAGGCCAAACAGCTCACGGTTGTAGGTTGCCAGTTTACAGAATTTCT TCTTGAATCTGAAGAGGATGGGCAAGGCTACTTAGAAGATCTAGTAAAGGATATTGTTCAGTGGCTCAAT GCTTCATCTGGAATGAAACCCGAAAGAAGTCTTCAAAATAATGGTTTATTGACCACCCTTAGTCAACACT ACTTTTTATTTATTGGAACACTTTCTTGCCACCCTCATGGAGTTAAAATGCTGGAAAAATGCAGTGTATT TCAGTGTCTCCTTAATCTTTGCTCCTTGAAAAACCAAGATCACTTGCTAAAACTTACTGTTTCTAGCTTG GACTATAGCAGAGATGGATTGGCTAGAGTCATCCTTTCCAAAATTTTAACTGCAGCTACTGATGCCTGCA GACTCTATGCAACAAAACATTTAAGGGTATTATTGAGAGCTAATGTTGAATTCTTTAATAATTGGGGAAT TGAGTTGTTAGTGACCCAGCTACATGATAAAAACAAAACGATTTCCTCTGAAGCTCTTGATATCCTCGAT GAAGCATGTGAAGACAAGGCCAATCTTCATGCTCTCATTCAGATGAAACCAGCGTTATCCCACCTTGGAG ACAAGGGTTTGCTTCTCCTGCTGAGATTTCTCTCCATTCCAAAAGGATTTTCCTATCTGAATGAAAGAGG TTATGTAGCAAAACAATTGGAAAAGTGGCACAGGGAATACAACTCCAAATATGTTGACTTGATTGAGGAA CAACTCAATGAAGCACTTACTACTTACCGGAAGCCTGTTGATGGTGATAACTATGTTCGTCGGAGTAACC AAAGATTACAGCGTCCTCACGTCTACCTGCCTATACACCTTTATGGACAACTAGTACACCATAAAACAGG CTGCCATTTGTTGGAAGTACAGAATATTATTACAGAACTCTGTCGTAATGTTCGTACACCAGATTTGGAT AAGTGGGAAGAAATTAAAAAACTGAAAGCATCTCTTTGGGCCTTGGGAAATATCGGCTCATCAAATTGGG GTCTCAATTTGCTACAGGAAGAAAACGTGATTCCAGATATACTAAAACTTGCAAAACAGTGTGAAGTTCT TTCCATCAGAGGGACCTGTGTATATGTACTTGGGCTCATAGCTAAAACCAAACAAGGCTGTGATATTCTA AAATGTCACAACTGGGATGCTGTGAGGCATAGTCGCAAACATCTGTGGCCAGTGGTTCCAGATGATGTGG AACAACTCTGTAATGAACTTTCATCTATCCCAAGCACTCTAAGTTTGAACTCGGAGTCAACCAGCTCTAG ACATAATAGTGAAAGTGAATCTGTGCCATCGAGTATGTTCATATTGGAGGATGACCGGTTTGGCAGCAGC TCTACTAGTACATTTTTCCTTGATATCAATGAAGATACAGAGCCAACATTTTATGACCGATCTGGACCCA TAAAGGATAAAAATTCATTCCCTTTCTTTGCTTCTAGTAAACTTGTGAAGAATCGTATCTTAAATTCGCT TACTTTGCCTAACAAAAAACATCGTAGTAGCAGTGATCCAAAAGGAGGGAAATTATCATCTGAAAGTAAG ACAAGCAACAGGCGAATCAGAACACTTACGGAGCCCAGTGTTGATTTTAATCATAGTGATGATTTTACAC CCATATCCACTGTACAGAAAACATTACAATTAGAGACTTCATTTATGGGGAATAAGCACATTGAAGACAC TGGTAGTACACCAAGCATTGGAGAAAATGACTTAAAATTCACCAAGAATTTTGGTACAGAGAATCACAGA GAAAATACAAGCCGAGAGAGGTTAGTAGTAGAAAGTTCAACGAGCTCACATATGAAGATACGTAGCCAAA GTTTCAATACAGACACTACAACAAGTGGCATAAGTTCAATGAGCTCAAGTCCTTCACGAGAGACAGTAGG TGTAGATGCTACAACTATGGACACAGACTGTGGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAG ACAAGCCACTATTTGACGCCACAGTCTAACCATCTGTCTCTCTCCAAATCAAATTCGGTGTCCCTGGTGC CTCCAGGTTCTTCTCATACGCTTCCTAGAAGAGCACAGTCCCTTAAAGCACCCTCTATTGCTACAATTAA AAGTCTAGCAGATTGTAACTTTAGTTACACAAGTTCTAGAGATGCTTTTGGCTATGCTACACTGAAAAGA CTACAGCAACAAAGAATGCATCCATCCTTATCTCACTCTGAAGCTTTGGCATCTCCAGCAAAAGATGTGC TATTTACTGATACCATCACCATGAAGGCCAACAGTTTTGAGTCCAGATTAACACCAAGCAGGTTCATGAA AGCCTTAAGTTATGCATCATTAGATAAAGAAGATTTATTGAGTCCTATTAATCAAAATACCCTGCAACGA TCTTCCTCAGTGCGGTCCATGGTGTCCAGTGCCACATATGGGGGTTCAGATGATTACATTGGTCTTGCTC TCCCGGTGGATATAAATGATATATTCCAGGTAAAGGATATTCCCTATTTTCAGACAAAAAACATACCACC ACATGATGATCGAGGTGCAAGAGCATTTGCCCATGATGCAGGAGAAAGTACAGAAGACACTGGACTACAG GAACATACAGATGATAACTGCCTTTATTGTGTCTGTATTGAAATTCTGGGTTTCCAGCCCAGCAACCAAC TGAGTGCAATATGTAGTCATTCAGACTTTCAAGATATTCCATATTCTGATTGGTGTGAGCAGACTATCCA TAATCCTTTAGAAGTGGTTCCCTCTAAGTTTTCGGGGATTTCTGGATGCAGTGATGGGGTGTCTCAAGAA GGCTCAGCTAGCAGCACCAAAAGCACAGAATTGTTACTAGGTGTTAAAACAATTCCAGATGATACACCAA TGTGCCGTATACTCCTTCGCAAAGAAGTTCTAAGATTAGTCATTAATTTGAGTAGTTCAGTTTCAACTAA ATGTCATGAGACTGGGCTTTTAACAATTAAGGAGAAGTATCCTCAAACATTTGATGACATATGCCTTTAC TCTGAGGTTTCCCATTTGCTGTCACACTGCACATTCAGACTTCCGTGTCGGAGGTTCATACAAGAATTAT TTCAAGATGTACAGTTTCTACAAATGCATGAAGAAGCAGAGGCTGTGTTGGCAACACCACCAAAGCAACC TATAGTTGATACATCTGCTGAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240129 representing NM_001285440 Red=Cloning site Green=Tags(s) MGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEALLSVDP GRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATI LLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPYSLHLD HIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLEWNWNLIGTILKWPNVNLRNY KDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLN ASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSSL DYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILD EACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHREYNSKYVDLIEE QLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVRTPDLD KWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQGCDIL KCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTSSRHNSESESVPSSMFILEDDRFGSS STSTFFLDINEDTEPTFYDRSGPIKDKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESK TSNRRIRTLTEPSVDFNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHR ENTSRERLVVESSTSSHMKIRSQSFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVSTKTIK TSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGYATLKR LQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINQNTLQR SSSVRSMVSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGESTEDTGLQ EHTDDNCLYCVCIEILGFQPSNQLSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQE GSASSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLY SEVSHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKQPIVDTSAES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285440
ORF Size	4155 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285440.1, NP_001272369.1
RefSeq Size	9441 bp
RefSeq ORF	4158 bp
Locus ID	253260
UniProt ID	Q6R327
Protein Pathways	mTOR signaling pathway
MW	156.3 kDa
Gene Summary	RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]

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