Pumilio 2 (PUM2) (NM_001282790) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PUMH2; PUML2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239854 representing NM_001282790 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAGCTTTTGCCTACCAAAAAGT TTTGGGAACCTGATGATTCAACAAAAGATGGACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGA GACTGCATGGGGAGCTTCTCACCATTCAATGTCCCAGCCTATTATGGTACAGAGAAGATCTGGACAGGGT TTTCATGGAAACAGTGAAGTAAATGCAATACTGTCTCCGCGATCAGAAAGTGGAGGCCTTGGTGTGAGCA TGGTAGAATATGTATTAAGTTCTTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAAATTTTGG CACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAG GACCAAAACAGAGATCTTAAACAAGGAGATGATGATGATTCTAAAATAAATGGCAGAGGTTTGCCAAATG GAATGGATGCCGATTGCAAAGATTTTAATCGTACTCCTGGAAGTCGTCAAGCCTCTCCAACTGAAGTAGT TGAGCGCTTGGGCCCCAATACTAATCCCTCAGAAGGACTGGGGCCTCTTCCTAATCCTACAGCTAATAAA CCACTTGTTGAAGAATTTTCAAATCCTGAAACTCAGAATCTGGATGCCATGGAACAAGTTGGTCTGGAAT CCTTACAGTTTGACTATCCTGGTAATCAGGTACCAATGGACTCTTCAGGAGCTACTGTAGGCCTTTTTGA CTACAATTCCCAGCAGCAGCTCTTTCAGAGGACTAATGCACTAACAGTTCAACAGTTAACTGCAGCTCAA CAGCAGCAATATGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTC CAGCTGCATTTGTGCCAAATCCATACATTATTAGTGCTGCTCCTCCAGGGACCGATCCGTATACTGCAGC AGGATTGGCTGCAGCAGCTACATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGCGTTCCATGG GGGGTGTATCCAGCCAACTTATTTCAGCAGCAAGCTGCAGCTGCGGCAAATAACACAGCCAGTCAGCAAG CAGCATCACAAGCTCAGCCTGGACAGCAACAGGTTCTCCGTGCTGGAGCAGGTCAGCGTCCTCTTACTCC CAATCAGGGTCAGCAAGGGCAGCAAGCAGAATCACTTGCGGCAGCTGCAGCAGCAAATCCAACATTGGCT TTTGGTCAGGGTCTTGCTACTGGCATGCCAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGA CTGGTGCCTTAGTGGTTGGCCCTGGAGCAAGGACTGGCCTTGGAGCTCCAGTTCGGTTAATGGCTCCAAC ACCTGTTTTAATTAGTTCAGCAGCAGCACAAGCTGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAGTAGC CTTACAGGCAGCACAAATGGTCTGTTTCGGCCAATTGGCACTCAGCCACCACAGCAGCAGCAACAGCAGC CAAGCACTAATCTGCAATCTAATTCATTTTATGGAAGCAGTTCTTTGACTAATAGCTCCCAGAGTAGTTC TTTATTTTCTCATGGACCTGGTCAACCTGGAAGTACATCTCTTGGCTTTGGAAGTGGTAACTCTTTGGGT GCTGCTATAGGCTCAGCCCTCAGTGGATTTGGTTCATCAGGAGGACTGACAAATGGTAGTGGTCGATATA TCTCTGCAGCACCTGGAGCAGAAGCAAAATATCGAAGTGCTTCAAGCACTTCCAGTCTATTTAGCTCCAG CAGCCAGCTCTTTCCTCCTTCCCGGCTTCGGTATAATAGGTCTGATATTATGCCTTCTGGCCGCAGTAGA TTATTGGAAGATTTCAGAAACAACCGCTTCCCAAACCTTCAGCTTAGAGACTTGATTGGACATATAGTTG AGTTTTCTCAAGACCAGCATGGTTCTAGATTCATACAGCAAAAACTAGAGAGAGCTACTCCAGCTGAGCG ACAGATGGTATTTAATGAAATTCTGCAAGCAGCCTATCAATTAATGACTGATGTTTTTGGCAACTATGTT ATACAGAAGTTTTTTGAGTTTGGGAGTCTGGATCAAAAATTAGCCCTGGCTACTCGTATTCGTGGTCATG TTCTACCCTTAGCCTTGCAGATGTATGGCTGCCGCGTTATTCAGAAAGCATTAGAATCTATTTCTTCTGA CCAGCAGAGTGAAATGGTAAAGGAGCTGGATGGTCATGTGCTCAAATGTGTGAAAGATCAGAATGGAAAC CATGTTGTACAAAAATGTATCGAATGTGTTCAGCCACAGTCACTACAGTTCATCATTGATGCTTTCAAGG GACAAGTATTTGTGCTTTCAACTCATCCTTATGGCTGCAGAGTAATTCAGCGCATCCTAGAGCATTGCAC TGCAGAACAGACCTTACCTATCTTAGAAGAACTCCACCAACATACAGAGCAGTTGGTACAGGATCAGTAT GGCAATTATGTTATTCAGCATGTACTGGAACACGGTCGACCTGAAGACAAGAGCAAAATTGTTTCCGAAA TCAGGGGAAAGGTTTTAGCCCTGAGTCAACACAAATTTGCCAGCAATGTAGTAGAAAAGTGTGTTACTCA TGCCTCCCGTGCTGAGAGAGCTTTACTGATTGACGAGGTTTGCTGCCAGAATGATGGTCCTCACAGTGCC TTATACACCATGATGAAGGACCAGTATGCCAATTACGTGGTTCAAAAGATGATTGATATGGCTGAACCTG CTCAGAGAAAGATAATCATGCACAAGATTCGACCTCACATTACTACTTTGCGCAAATACACATACGGGAA GCATATACTGGCCAAGTTGGAAAAGTATTATTTGAAGAATAGCCCGGACCTAGGACCTATTGGAGGACCA CCAAATGGAATGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239854 representing NM_001282790 Red=Cloning site Green=Tags(s) MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGASHHSMSQPIMVQRRSGQG FHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGNFGTRDAETDGPEKGDQKGKASPFEE DQNRDLKQGDDDDSKINGRGLPNGMDADCKDFNRTPGSRQASPTEVVERLGPNTNPSEGLGPLPNPTANK PLVEEFSNPETQNLDAMEQVGLESLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTNALTVQQLTAAQ QQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATLAGPAVVPPQYYGVPW GVYPANLFQQQAAAAANNTASQQAASQAQPGQQQVLRAGAGQRPLTPNQGQQGQQAESLAAAAAANPTLA FGQGLATGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTPVLISSAAAQAAAAAAAGGTASS LTGSTNGLFRPIGTQPPQQQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSTSLGFGSGNSLG AAIGSALSGFGSSGGLTNGSGRYISAAPGAEAKYRSASSTSSLFSSSSQLFPPSRLRYNRSDIMPSGRSR LLEDFRNNRFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAAYQLMTDVFGNYV IQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVLKCVKDQNGN HVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQY GNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSA LYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYYLKNSPDLGPIGGP PNGML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282790
ORF Size	2955 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282790.2
RefSeq Size	5877 bp
RefSeq ORF	2958 bp
Locus ID	23369
MW	106.5 kDa
Gene Summary	This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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