KCNH6 (NM_001278920) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ERG-2; ERG2; hERG-2; HERG2; Kv11.2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239652 representing NM_001278920 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCATTCTCAACTTCGAGGACCTGGCCCAGCTCCTGGCCAAGTGCAGCAGCCGCAGCTTGTCCCAGC GCCTGTTGTCCCAGAGCTTCCTGGGCTCCGAGGGCTCTCATGGCAGGCCAGGCGGACCAGGGCCAGGCAC AGGCAGGGGCAAGTACAGGACCATCAGCCAGATCCCACAGTTCACGCTCAACTTCGTGGAGTTCAACTTG GAGAAGCACCGCTCCAGCTCCACCACGGAGATTGAGATCATCGCGCCCCATAAGGTGGTGGAGCGGACAC AGAACGTCACTGAGAAGGTCACCCAGGTCCTGTCCCTGGGCGCGGATGTGCTGCCGGAGTACAAGCTGCA GGCGCCGCGCATCCACCGCTGGACCATCCTGCACTACAGCCCCTTCAAGGCCGTGTGGGACTGGCTCATC CTGCTGCTGGTCATCTACACGGCTGTCTTCACGCCCTACTCAGCCGCCTTCCTGCTCAGCGATCAGGACG AATCACGGCGTGGGGCCTGCAGCTATACCTGCAGTCCCCTCACTGTGGTGGATCTCATCGTGGACATCAT GTTCGTCGTGGACATCGTCATCAACTTCCGCACCACCTATGTCAACACCAATGATGAGGTGGTCAGCCAC CCCCGCCGCATCGCCGTCCACTACTTCAAGGGCTGGTTCCTCATTGACATGGTGGCCGCCATCCCTTTCG ACCTCCTGATCTTCCGCACTGGCTCCGATGAGACCACAACCCTGATTGGGCTATTGAAGACAGCGCGGCT GCTGCGGCTGGTGCGCGTAGCACGGAAGCTGGACCGCTACTCTGAGTATGGGGCGGCTGTGCTCTTCTTG CTCATGTGCACCTTCGCGCTCATAGCGCACTGGCTGGCCTGCATCTGGTACGCCATCGGCAATGTGGAGC GGCCCTACCTAGAACACAAGATCGGCTGGCTGGACAGCCTGGGTGTGCAGCTTGGCAAGCGCTACAACGG CAGCGACCCAGCCTCGGGCCCCTCGGTGCAGGACAAGTATGTCACAGCCCTCTACTTCACCTTCAGCAGC CTCACCAGCGTGGGCTTCGGCAATGTCTCGCCCAACACCAACTCCGAGAAGGTCTTCTCCATCTGCGTCA TGCTCATCGGCTCCCTGATGTACGCCAGCATCTTCGGGAACGTGTCCGCGATCATCCAGCGCCTGTACTC GGGCACCGCGCGCTACCACACGCAGATGCTGCGTGTCAAGGAGTTCATCCGCTTCCACCAGATCCCCAAC CCACTGCGCCAGCGCCTGGAGGAGTATTTCCAGCACGCCTGGTCCTACACCAATGGCATTGACATGAACG CGGTGCTGAAGGGCTTCCCCGAGTGCCTGCAGGCTGACATCTGCCTGCACCTGCACCGCGCACTGCTGCA GCACTGCCCAGCTTTCAGCGGCGCCGGCAAGGGCTGCCTGCGCGCGCTAGCCGTCAAGTTCAAGACCACC CACGCGCCGCCTGGGGACACGCTGGTGCACCTCGGCGACGTGCTCTCCACCCTCTACTTCATCTCCCGAG GCTCCATCGAGATCCTGCGCGACGACGTGGTCGTGGCCATCCTAGGAAAGAATGACATCTTTGGGGAACC CGTCAGCCTCCATGCCCAGCCAGGCAAGTCCAGTGCAGACGTGCGGGCTCTGACCTACTGCGACCTGCAC AAGATCCAGCGGGCAGATCTGCTGGAGGTGCTGGACATGTACCCGGCCTTTGCGGAGAGCTTCTGGAGTA AGCTGGAGGTCACCTTCAACCTGCGGGACGCAGCCGGGGGTCTCCACTCATCCCCCCGACAGGCTCCTGG CAGCCAAGACCACCAAGGTTTCTTTCTCAGTGACAACCAGTCAGATGCAGCCCCTCCCCTGAGCATCTCA GATGCATCTGGCCTCTGGCCTGAGCTACTGCAGGAAATGCCCCCAAGGCACAGCCCCCAAAGCCCTCAGG AAGACCCAGATTGCTGGCCTCTGAAGCTGGGCTCCAGGCTAGAGCAGCTCCAGGCCCAGATGAACAGGCT GGAGTCCCGCGTGTCCTCAGACCTCAGCCGCATCTTGCAGCTCCTCCAGAAGCCCATGCCCCAGGGCCAC GCCAGCTACATTCTGGAAGCCCCTGCCTCCAATGACCTGGCCTTGGTTCCTATAGCCTCGGAGACGACGA GTCCAGGGCCCAGGCTGCCCCAGGGCTTTCTGCCTCCTGCACAGACCCCAAGCTATGGAGACTTGGATGA CTGTAGTCCAAAGCACAGGAACTCCTCCCCCAGGATGCCTCACCTGGCTGTGGCAACGGACAAAACTCTG GCACCATCCTCAGAACAGGAACAGCCTGAGGGGCTCTGGCCACCCCTAGCCTCACCTCTACATCCCCTGG AAGTACAAGGACTCATCTGTGGTCCCTGCTTCTCCTCCCTCCCTGAACACCTTGGCTCTGTTCCCAAGCA GCTGGACTTCCAGAGACATGGCTCAGATCCTGGATTTGCAGGGAGTTGGGGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239652 representing NM_001278920 Red=Cloning site Green=Tags(s) MFILNFEDLAQLLAKCSSRSLSQRLLSQSFLGSEGSHGRPGGPGPGTGRGKYRTISQIPQFTLNFVEFNL EKHRSSSTTEIEIIAPHKVVERTQNVTEKVTQVLSLGADVLPEYKLQAPRIHRWTILHYSPFKAVWDWLI LLLVIYTAVFTPYSAAFLLSDQDESRRGACSYTCSPLTVVDLIVDIMFVVDIVINFRTTYVNTNDEVVSH PRRIAVHYFKGWFLIDMVAAIPFDLLIFRTGSDETTTLIGLLKTARLLRLVRVARKLDRYSEYGAAVLFL LMCTFALIAHWLACIWYAIGNVERPYLEHKIGWLDSLGVQLGKRYNGSDPASGPSVQDKYVTALYFTFSS LTSVGFGNVSPNTNSEKVFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVKEFIRFHQIPN PLRQRLEEYFQHAWSYTNGIDMNAVLKGFPECLQADICLHLHRALLQHCPAFSGAGKGCLRALAVKFKTT HAPPGDTLVHLGDVLSTLYFISRGSIEILRDDVVVAILGKNDIFGEPVSLHAQPGKSSADVRALTYCDLH KIQRADLLEVLDMYPAFAESFWSKLEVTFNLRDAAGGLHSSPRQAPGSQDHQGFFLSDNQSDAAPPLSIS DASGLWPELLQEMPPRHSPQSPQEDPDCWPLKLGSRLEQLQAQMNRLESRVSSDLSRILQLLQKPMPQGH ASYILEAPASNDLALVPIASETTSPGPRLPQGFLPPAQTPSYGDLDDCSPKHRNSSPRMPHLAVATDKTL APSSEQEQPEGLWPPLASPLHPLEVQGLICGPCFSSLPEHLGSVPKQLDFQRHGSDPGFAGSWGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278920
ORF Size	2505 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278920.2
RefSeq Size	3650 bp
RefSeq ORF	2508 bp
Locus ID	81033
UniProt ID	Q9H252
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
MW	93.2 kDa
Gene Summary	Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

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