SMARCA6 (HELLS) (NM_001289068) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ICF4; LSH; Nbla10143; PASG; SMARCA6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239634 representing NM_001289068 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGAACAACTGGACACTGCTGTGATTACCCCGGCCATGCTAGAAGAGGAAGAACAGCTTGAAGCTG CTGGACTAGAGAGAGAGCGGAAGATGCTGGAAAAGGCTCGCATGTCTTGGGATAGAGAGTCGACAGAAAT TCGGTACCGTAGACTTCAACATTTGCTTGAAAAAAGCAATATATACTCCAAATTTTTATTGACGAAAATG GAACAGCAACAATTAGAGGAACAGAAGAAGAAAGAAAAATTGGAGAGAAAAAAGGAGTCTTTAAAAGTTA AAAAGGGTAAAAATTCAATTGATGCAAGTGAAGAGAAGCCAGTTATGAGGAAAAAAAGAGGAAGAGAAGA TGAATCATACAATATTTCAGAGGTCATGTCAAAAGAGGAAATTTTGTCTGTGGCTAAAAAAAATAAAAAG GAGAATGAGGATGAAAACTCCTCCTCTACTAATCTCTGTGTGGAAGATCTTCAGAAAAATAAAGATTCGA ATAGTATAATTAAAGATAGATTGTCTGAAACGGTTAGGCAGAATACTAAATTCTTTTTTGACCCAGTCCG GAAGTGTAATGGTCAGCCAGTACCTTTTCAACAACCAAAGCACTTCACTGGAGGAGTGATGCGATGGTAC CAAGTAGAAGGCATGGAATGGCTTAGGATGCTTTGGGAAAATGGAATTAATGGCATTTTAGCAGATGAAA TGGGATTGGGTAAGACAGTTCAGTGCATTGCTACTATTGCATTGATGATTCAGAGAGGAGTACCAGGACC TTTTCTTGTCTGTGGCCCTTTGTCTACACTTCCTAACTGGATGGCTGAATTCAAAAGATTTACACCAGAT ATCCCTACAATGTTATATCATGGAACCCAGGAGGAACGTCAAAAATTGGTAAGAAATATTTACAAACGGA AAGGGACTTTGCAGATTCATCCTGTGGTAATCACGTCATTTGAAATAGCCATGAGAGACCGAAATGCGTT ACAGCATTGCTATTGGAAATACTTAATAGTAGATGAAGGACACAGGATTAAGAATATGAAGTGCCGTCTA ATCAGGGAGTTAAAACGATTCAATGCTGATAACAAACTTCTTTTGACTGGTACTCCCTTGCAAAACAATT TATCAGAACTTTGGTCATTGCTAAACTTTTTGTTGCCAGATGTATTTGATGACTTGAAAAGCTTTGAGTC TTGGTTTGACATCACTAGTCTTTCTGAAACTGCTGAAGATATTATTGCTAAAGAAAGAGAACAGAATGTA TTGCATATGCTGCACCAGATTTTAACACCTTTCTTATTGAGAAGACTGAAGTCTGATGTTGCTCTTGAAG TTCCTCCTAAACGAGAAGTAGTCGTTTATGCTCCACTTTCAAAGAAGCAGGAGATCTTTTATACAGCCAT TGTGAACCGTACAATTGCAAACATGTTTGGATCCAGTGAGAAAGAAACAATTGAGTTAAGTCCTACTGGT CGACCAAAACGACGAACTAGAAAATCAATAAATTACAGCAAAATAGATGATTTCCCTAATGAATTGGAAA AACTGATCAGTCAAATACAGCCAGAGGTGGACCGAGAAAGAGCTGTTGTGGAAGTGAATATCCCTGTAGA ATCTGAAGTTAATCTGAAGCTGCAGAATATAATGATGCTACTTCGTAAATGTTGTAATCATCCATATTTG ATTGAATATCCTATAGACCCTGTTACACAAGAATTTAAGATCGATGAAGAATTGGTAACAAATTCTGGGA AGTTCTTGATTTTGGATCGAATGCTGCCAGAACTAAAAAAAAGAGGTCACAAGGTGCTGCTTTTTTCACA AATGACAAGCATGTTGGACATTTTGATGGATTACTGCCATCTCAGAGATTTCAACTTCAGCAGGCTTGAT GGGTCCATGTCTTACTCAGAGAGAGAAAAAAACATGCACAGCTTCAACACGGATCCAGAGGTGTTTATCT TCTTAGTGAGTACACGAGCTGGTGGCCTGGGCATTAATCTGACTGCAGCAGATACAGTTATCATTTATGA TAGTGATTGGAACCCCCAGTCGGATCTTCAGGCCCAGGATAGATGTCATAGAATTGGTCAGACAAAGCCA GTTGTTGTTTATCGCCTTGTTACAGCAAATACTATCGATCAGAAAATTGTGGAAAGAGCAGCTGCTAAAA GGAAACTGGAAAAGTTGATCATCCATAAAAATCATTTCAAAGGTGGTCAGTCTGGATTAAATCTGTCTAA GAATTTCTTAGATCCTAAGGAATTAATGGAATTATTAAAATCTAGAGATTATGAAAGGGAAATAAAAGGA TCAAGAGAGAAGGTCATTAGTGATAAAGATCTAGAGTTGTTGTTAGATCGAAGTGATCTTATTGATCAAA TGAATGCTTCAGGACCAATTAAAGAGAAGATGGGGATATTCAAGATATTAGAAAATTCTGAAGATTCCAG TCCTGAATGTTTGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239634 representing NM_001289068 Red=Cloning site Green=Tags(s) MVEQLDTAVITPAMLEEEEQLEAAGLERERKMLEKARMSWDRESTEIRYRRLQHLLEKSNIYSKFLLTKM EQQQLEEQKKKEKLERKKESLKVKKGKNSIDASEEKPVMRKKRGREDESYNISEVMSKEEILSVAKKNKK ENEDENSSSTNLCVEDLQKNKDSNSIIKDRLSETVRQNTKFFFDPVRKCNGQPVPFQQPKHFTGGVMRWY QVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPLSTLPNWMAEFKRFTPD IPTMLYHGTQEERQKLVRNIYKRKGTLQIHPVVITSFEIAMRDRNALQHCYWKYLIVDEGHRIKNMKCRL IRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNV LHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLSKKQEIFYTAIVNRTIANMFGSSEKETIELSPTG RPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPYL IEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSRLD GSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKP VVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKG SREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289068
ORF Size	2466 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289068.2
RefSeq Size	3118 bp
RefSeq ORF	2469 bp
Locus ID	3070
UniProt ID	Q9NRZ9
MW	96.1 kDa
Gene Summary	This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]

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