PIF1 (NM_001286496) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C15orf20; PIF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239183 representing NM_001286496 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTCGGGCATAGAGGCGGCGGCAGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGTGG AGGAGCTGAGCCCGGGCGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCTGAGCCTGGGTCG CAACGAGCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGCGGGGCGGCCGCGCTGCTTCCCT CTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGCCGGGCGCAGCACCCTGCGGCTCCCCGCCCACG ACACCCCCGGGGCCGGCGCAGTGCAGCTGCTGCTCTCGGACTGCCCCCCAGACCGCCTGCGCCGCTTCCT GCGCACATTGCGCCTCAAGCTGGCTGCGGCCCCGGGTCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTG GGCCCGCGGCCCCGCGACTTCGTCACCATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGCCA CCCGGGTTCCGGACACTACGCTGGTGAAGCGGCCTGTGGAGCCCCAGGCTGGGGCCGAGCCTAGCACAGA AGCCCCAAGGTGGCCCCTGCCTGTGAAGAGGCTGAGCTTGCCCTCCACCAAGCCACAGCTTTCTGAGGAA CAGGCTGCTGTGCTGAGGGCCGTCCTGAAAGGCCAGAGCATCTTCTTCACTGGGAGTGCAGGAACAGGGA AGTCATATCTGCTAAAGCGAATCCTGGGCTCACTGCCCCCCACAGGCACTGTGGCCACTGCCAGCACTGG GGTGGCAGCCTGCCACATCGGGGGCACCACCCTCCATGCCTTTGCAGGCATCGGCTCAGGCCAGGCTCCT CTAGCCCAGTGTGTGGCCCTGGCCCAAAGGCCAGGCGTGCGGCAGGGCTGGCTGAACTGCCAGCGGTTGG TCATTGACGAGATCTCAATGGTGGAGGCAGACCTGTTTGACAAACTGGAGGCCGTGGCCAGAGCTGTCCG GCAGCAGAACAAGCCATTCGGAGGGATCCAGCTCATCATCTGTGGGGACTTTCTGCAGCTGCCACCTGTG ACCAAGGGCTCCCAGCCCCCACGGTTCTGCTTCCAGTCCAAGAGCTGGAAGAGGTGTGTGCCAGTGACCC TGGAGCTGACCAAGGTGTGGAGGCAGGCAGACCAGACCTTCATCTCTCTACTGCAGGCCGTGAGGCTAGG CAGGTGTTCAGATGAGGTGACCCGCCAGCTCCAGGCCACAGCTTCCCACAAGGTGGGGCGAGATGGGATT GTGGCCACGAGGCTCTGCACCCACCAGGATGATGTGGCCCTCACCAACGAGAGGCGGCTTCAGGAGCTGC CAGGTAAGGTACACAGATTTGAGGCTATGGACAGCAACCCTGAGCTGGCCAGTACCCTGGATGCCCAGTG TCCTGTTAGCCAGCTCCTTCAACTAAAGCTGGGGGCCCAGGTGATGCTGGTGAAAAACTTATCGGTGTCT CGGGGCCTGGTGAATGGTGCCCGAGGGGTGGTAGTTGGGTTCGAGGCAGAAGGGAGAGGGCTACCCCAGG TGCGGTTCCTGTGTGGAGTCACTGAGGTCATCCACGCTGACCGCTGGACGGTGCAGGCCACCGGGGGCCA GCTCCTCAGTCGGCAGCAGCTGCCCCTCCAGCTGGCCTGGGCGATGTCCATCCACAAGAGCCAAGGCATG ACCCTGGATTGTGTGGAGATTTCTCTGGGCCGTGTGTTTGCCAGTGGCCAGGCCTATGTGGCCCTTTCTC GGGCCCGCAGCCTGCAGGGCCTACGTGTGCTGGACTTTGACCCCATGGCGGTTCGCTGTGACCCCCGTGT GCTGCACTTCTATGCCACCCTGCGGCGGGGCAGGAGCCTCAGTCTGGAGTCCCCAGATGATGATGAGGCA GCCTCAGACCAGGAGAACATGGACCCAATCCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239183 representing NM_001286496 Red=Cloning site Green=Tags(s) MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFP LRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLL GPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEE QAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAP LAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPV TKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGI VATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVS RGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGM TLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEA ASDQENMDPIL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286496
ORF Size	1923 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286496.1, NP_001273425.1
RefSeq Size	2718 bp
RefSeq ORF	1926 bp
Locus ID	80119
UniProt ID	Q9H611
MW	69.8 kDa
Gene Summary	This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013]

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